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Detection of Iron Nanoparticles using Nuclear Magnetic Resonance Relaxometry and Inverse Laplace Transform

  • Kim, Seong Min
    • Journal of Biosystems Engineering
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    • v.39 no.4
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    • pp.345-351
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    • 2014
  • Purpose: Rapid detection of bacteria is very important in agricultural and food industries to prevent many foodborne illnesses. The objective of this study was to develop a portable nuclear magnetic resonance (NMR)-based system to detect foodborne pathogens (E. coli). This study was focused on developing a method to detect low concentrations of magnetic nanoparticles using NMR techniques. Methods: NMR relaxometry was performed to examine the NMR properties of iron nanoparticle mixtures with different concentrations by using a 1 T permanent magnet magnetic resonance imaging system. Exponential curve fitting (ECF) and inverse Laplace transform (ILT) methods were used to estimate the NMR relaxation time constants, $T_1$ and $T_2$, of guar gum solutions with different iron nanoparticle concentrations (0, $10^{-3}$, $10^{-4}$, $10^{-5}$, $10^{-6}$, and $10^{-7}M$). Results: The ECF and ILT methods did not show much difference in these values. Analysis of the NMR relaxation data showed that the ILT method is comparable to the classical ECF method and is more sensitive to the presence of iron nanoparticles. This study also showed that the spin-spin relaxation time constants acquired by a Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence are more useful for determining the concentration of iron nanoparticle solutions comparwith the spin-lattice relaxation time constants acquired by an inversion recovery pulse sequence. Conclusions: We conclude that NMR relaxometry that utilizes CPMG pulse sequence and ILT analysis is more suitable for detecting foodborne pathogens bound to magnetic nanoparticles in agricultural and food products than using inversion recovery pulse sequence and ECF analysis.

Immediate effect of Nd:YAG laser monotherapy on subgingival periodontal pathogens: a pilot clinical study

  • McCawley, Thomas K.;McCawley, Mark N.;Rams, Thomas E.
    • Journal of Periodontal and Implant Science
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    • v.52 no.1
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    • pp.77-87
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    • 2022
  • Purpose: This pilot study assessed the immediate in vivo effect of high peak pulse power neodymium-doped yttrium aluminum garnet (Nd:YAG) laser monotherapy on selected red/orange complex periodontal pathogens in deep human periodontal pockets. Methods: Twelve adults with severe periodontitis were treated with the Laser-Assisted New Attachment Procedure (LANAP®) surgical protocol, wherein a free-running, digitally pulsed, Nd:YAG dental laser was used as the initial therapeutic step before mechanical root debridement. Using a flexible optical fiber in a handpiece, Nd:YAG laser energy, at a density of 196 J/cm2 and a high peak pulse power of 1,333 W/pulse, was directed parallel to untreated tooth root surfaces in sequential coronal-apical passes to clinical periodontal probing depths, for a total applied energy dose of approximately 8-12 joules per millimeter of periodontal probing depth at each periodontal site. Subgingival biofilm specimens were collected from each patient before and immediately after Nd:YAG laser monotherapy from periodontal pockets exhibiting ≥6 mm probing depths and bleeding on probing. Selected red/orange complex periodontal pathogens (Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia/nigrescens, Fusobacterium nucleatum, Parvimonas micra, and Campylobacter species) were quantified in the subgingival samples using established anaerobic culture techniques. Results: All immediate post-treatment subgingival biofilm specimens continued to yield microbial growth after Nd:YAG laser monotherapy. The mean levels of total cultivable red/orange complex periodontal pathogens per patient significantly decreased from 12.0% pretreatment to 4.9% (a 59.2% decrease) immediately after Nd:YAG laser monotherapy, with 3 (25%) patients rendered culture-negative for all evaluated red/orange complex periodontal pathogens. Conclusions: High peak pulse power Nd:YAG laser monotherapy, used as the initial step in the LANAP® surgical protocol on mature subgingival biofilms, immediately induced significant reductions of nearly 60% in the mean total cultivable red/orange complex periodontal pathogen proportions per patient prior to mechanical root instrumentation and the rest of the LANAP® surgical protocol.

Gender-Related Differences in the Second Derivative of Photoplethysmogram Waveforms in the Fourth Decade (30대 성인남녀의 성별에 따른 가속도맥파 차이에 관한 연구)

  • 남동현;박연재;박영배
    • The Journal of Korean Medicine
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    • v.23 no.3
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    • pp.1-10
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    • 2002
  • Objective: There exist gender differences in pulsatile contour waveform. Women have a greater age-related increase in left ventricular mass than do men and more likely to experience symptomatic heart failure after infarction. SDPTG (the second derivative of photoplethysmogram waveform) is a noninvasive method for evaluating the pulse wave and is correlated with age and other risk factors for atherosclerosis. We studied the effect of gender on SDPTG and made clear why the gender differences appear. Methods: To study the effects of effect factors, including height and blood pressure, on SDPTG in the fourth decade, data on height, weight, PTD (pulse transit distance), blood pressure, serum lipid levels, and SDPTG were collected in 115 laboratory healthy men and women. SDPTG is derived from double-differential processing of fingertip photoplethysmography and consists of a, b, c, and d waves in systole and an e wave in diastole; SDPTG aging index (AI) was calculated as (b-c-d-e)/a. Results: There were significant gender-related differences of SDPTG AI, height, and blood pressure. Age, height, and mean blood pressure were respectively and significantly correlated with SDPTG AI. SDPTG is dependent upon age, height, and blood pressure. Restricting analysis to SDPTG AI, age, height, and mean blood pressure, yielded that there were gender-related differences in SDPTG AI (P<0.05) which were derived from those of height (F<0.001, df=l, P=0.994). Conclusions: These new data may help to explain previous findings about age-related differences in pulsatile contour waveforms and why gender differences of SDPTG appear. The results of this study suggest that SDPTG AI, used for evaluation of biological vascular aging, should be calibrated by height as well as age and blood pressure.

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A study on the optimization design of pulse air jet system to improve bag-filter performance (여과집진기의 탈진 성능 향상을 위한 충격 기류 분사 시스템 최적화 설계에 관한 연구)

  • Hong, Sung-Gil;Jung, Yu-Jin;Park, Ki-Woo;Jeong, Moon-Heon;Lim, Ki-Hyuk;Suh, Hye-Min;Shon, Byung-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.8
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    • pp.3792-3799
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    • 2012
  • The dedusting characteristics of pulse air jet type dedusting system which is widely applied in the industries were identified by utilizing the computational fluid dynamics (CFD) and the dedusting performance in modified shape of dedusting unit was compared in this study. The review on the dedusting air volume, air stream distribution and inflow velocity distribution on each shape of dedusting part showed that the case of installing the nozzle on the blow tube (Case-3) and the case of installing the double intaking tube to the venturi (Case-4 and Case-5) were more excellent than the structure (Case-1) which is widely applied in the field in its amplification effect on the air volume and extension of stream width. The specification of venturi was decided to apply the selected Case-5 for the option of the commercial back filter. It is considered that the dedusting air volume will be maintained in maximum in the case of 50 mm and 90 mm for the diameter of internal and external intaking pipe respectively.

The thermal analysis of te-based media for the optical recording (광기록에 이용되는 Te-based media에 대한 열적 해석)

  • 이성준;천석표;이현용;정홍배
    • Electrical & Electronic Materials
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    • v.8 no.1
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    • pp.64-70
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    • 1995
  • We discussed the thermal analysis for a recording media with the variation of the laser pulse duration, the laser power and the temperature distribution in order to optimize the Te-based antireflection structure from the computer calculations. In the case that the radial heat diffusion is negligible, we can calculate the maximum temperature of the recording layer at the center of the spot by the Simple Model. The temperature profile of the recording layer is obtained from the Numerical Model by considering the total specific heat and the latent heat. As a result, the effect of the heat sinking acting as a thermal loss for the hole formation could be minimized by introducing the pulse with the hole formation duration(.tau.) below the thermal time constant(.tau.$_{D}$) of a dielectric layer. These requirments can be satisfied by using the dielectric thickness of the 2nd ART(Anti-Reflection Trilayer) condition or the dielectric materials with a low thermal diffusivity.y.

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Characteristics Evaluation for a PZT Transducer with a Cylindrical Rear Surface (원통형 배면체를 갖는 PZT 트랜스듀서의 특성평가)

  • Kim, Dong-Hyun;Han, Jin-Ho;Yang, Jeong-Won;Kim, Moo-Joon;Ha, Kang-Lyeol
    • The Journal of the Acoustical Society of Korea
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    • v.26 no.1E
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    • pp.14-20
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    • 2007
  • The characteristics of a PZT transducer with a cylindrical rear surface were investigated theoretically and experimentally. The transducer was assumed as a set of finite rectangular ceramic elements for applying the staircase model to the theoretical analysis and the KLM equivalent circuit model was modified for analyzing its electric impedance and pulse-echo response. All results were compared with those of a conventional plane transducer which has a constant thickness of average value for the cylindrical arc. It was noted that the transducer with a cylindrical rear surface has several subsidiary resonant frequencies which are able to widen bandwidth.

Prevalence and Characterization of Vancomycin-Resistant Enterococci in Chicken Intestinles and Humans of Korea

  • Seong, Chi-Nam;Shim, Eun-Sook;Kim, Shin-Moo;Yoo, Jin-Cheol
    • Archives of Pharmacal Research
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    • v.27 no.2
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    • pp.246-253
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    • 2004
  • The prevalence, genotype for antibiotic resistance and antibiotic susceptibility of vancomycin resistant enterococci (VRE) were determined. And molecular typings of the Enterococcus faecium isolates were analyzed. Prevalence of VRE in chickens, healthy children and intensive care unit (ICU) patients was 41.6%,7.9%, and 20.4%, respectively. Forty out of 54 isolates from chicken intestines, and 9 out of 11 from ICU patients were identified as Enterococcus faecium. Eleven out of 13 isolates from non-hospitalized young children were E. gallinarium. Twelve strains of E. faecalis were isolated from chicken intestines. The gene for the antibiotic resistance in E. faecium, and E. faecalis was vanA, while that in E. gallinarium was vanC1. E. faecium isolates were resistant to most of antibiotics except ampicillin and gentamicin. Molecular typing of the E. faecium strains obtained by pulse field gel electrophoresis and repetitive sequence-based PCR suggest that VRE transmit horizontally from poultry to humans, especially young children, via the food chains in Korea.

Synthesis of Pentadentate Schiff Base Molybdenum(Ⅴ) Complexes and Their Electrochemical Properties in Aprotic Solvents (다섯자리 Schiff Base Molybdenum(Ⅴ) 착물들의 합성과 비수용매에서의 전기화학적 성질)

  • Kim, Seon Suk;Choe, Ju Hyeong;Choe, Yong Guk;Jeong, Byeong Gu
    • Journal of the Korean Chemical Society
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    • v.38 no.2
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    • pp.160-168
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    • 1994
  • Pentadentate Schiff base molybdenum(Ⅴ) complexes such as [Mo(Ⅴ)O(Sal-DET)(NCS)] and [Mo(Ⅴ)O(Sal-DPT)(NCS)] were synthesized by Sabat method. The structure of these complexes were identified by elemental analysis, spectroscopy, and thermogravimetric analysis(T.G.A.). It was found that the mole ratio of Schiff base ligand to the complexes was found to be 1 : 1. The redox processes of the complexes were investigated by cyclic voltammetric and differential pulse polarographic technique in nonaqueous solvent containing 0. 1 M tetraethyl ammonium perchlorate(TEAP) as supporting electrolyte at glassy carbon electrode. It was found that diffusion controlled reduction processes of four steps with one electron were 2Mo(Ⅴ)$\rightleftarrow^{e-}$ Mo(Ⅴ)Mo(Ⅳ) $\longrightarrow^{e-}$ 2Mo(Ⅳ), Mo(Ⅳ) $\longrightarrow^{e-}$ Mo(Ⅲ) $\longrightarrow^{e-}$ Mo(Ⅱ)

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The Characteristic Analysis of E.M.F. Induced by Moving Normal Spot in a Cylindrical Superconducting Foil

  • Kim, Ho-Min;Bae, Joon-Han;Yoon, Yong-Soo;Chu, Yong;Ko, Tae-Kuk;Han, Tae-Su
    • Progress in Superconductivity
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    • v.1 no.2
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    • pp.99-104
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    • 2000
  • The e.m.f. induced by a normal spot moving in a superconducting foil has been investigated by using the simulation and the experiment of a simple superconducting Power Supply. The induced e.m.f. has been derived theoretically from the magnetic field distribution within the spot. It is the sum of a DC component induced constantly by the Faraday's law during the spot's movement and a pulse component induced periodically by the flux conservation law at every electrical degrees 2$\pi$ radians. The DC component of the output voltage appears slightly nonlinear to the rotating speed, having values greater than the linear approximation values. The theoretical interpretation has been verified through experiment.

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Design of L-Band High Speed Pulsed High Power Amplifier Using LDMOS FET (LDMOS FET를 이용한 L-대역 고속 펄스 고전력 증폭기 설계)

  • Yi, Hui-Min;Hong, Sung-Yong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.4
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    • pp.484-491
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    • 2008
  • In this paper, we design and fabricate the L-band high speed pulsed HPA using LDMOS FET. And we propose the high voltage and high speed switching circuit for LDMOS FET. The pulsed HPA using LDMOS FET is simpler than using GaAs FET because it has a high gain, high output power and sin81e voltage supply. LDMOS FET is suitable for pulsed HPA using switching method because it has $2{\sim}3$ times higher maximum drain-source voltage(65 V) than operating drain-source voltage($V_{ds}=26{\sim}28\;V$). As results of test, the output peak power is 100 W at 1.2 GHz, the rise/fall time of output RF pulse are 28.1 ns/26.6 ns at 2 us pulse width with 40 kHz PRF, respectively.