• Korean Journal of Microbiology
• /
• v.26 no.1
• /
• pp.32-36
• /
• 1988

The yeast gene HOM3 encodes aspartokinase, which catalyses the first step (aspartate to and from beta-aspartyl phosphate) of common pathway to threonine and methionine. The yeast HOM3 gene expression is known to be regulated by threonine and methionine specific control, and also by general control of amino acid biosynthesis. Isolation and characterization of the HOM3 gene are essential for the molecular genetic study on its regulation of expression. A recombinant plasmid pSC3 (15.5kb, vector YCp50) has been cloned into E. coli HB101 from yeast genomic library through their complementing activity of HOM3 mutation in a yeast recipient strain M34-24B. Organization of the plasmid was characterized by delineation of restriction cleavage sites in the insert fragment.

• Proceedings of the KIEE Conference
• /
• 1993.07a
• /
• pp.406-409
• /
• 1993

Integration of intelligent robot workcell is now a hot issue in CIM and robotics area. This paper dealt with relatively low level essential topics. i. e., multi-robot coordination and real-time communication for the integration of intelligent robot workcell. For the coordination of multi-robot system, the tightly-coupled coordination is proposed using the various sensors. In order to handle the numerous communication data, time-critical communication network (field-bus) is introduced and investigated. Finally, intelligent robot workcell is suggested using the mini-MAP and field-bus.

• Industrial Engineering and Management Systems
• /
• v.2 no.1
• /
• pp.28-34
• /
• 2003

The fundamental design issues that arise in the pickup and delivery system planning are optimizing the system with minimum cost and maximum throughput and service level. This study is concerned with the development of pickup and delivery system with customer responsive service level, DCM(Demand Chain Management). The distribution process and service map are consisted of manufacturing, warehousing, and pickup and delivery. First we formulated the vehicle pickup and delivery problem using GIS-VRP method so as to satisfy the customer service requests. Second, we developed a GUI-type computer program using AVL, automated vehicle location system. The computational results show that the proposed method is very effective on a set of test problems.

• Annual Conference of KIPS
• /
• 2001.04a
• /
• pp.205-208
• /
• 2001

본 논문은 KCC 정보통신(주) e-RoadMap 웹 방법론에 적용한 컨텐츠, 데이터, 사용자인터페이스 모델이다. 이는 널리 알려진 구조적기법에 웹 어플리케이션을 개발하는 절차를 반영한 모델이다. 그러므로 기존의 방법론 사용자들에게 이해가 용이하다. 이벤트리스트와 웹 시나리오요구서를 통하여 사용자요구정의를 하고, 컨텐츠 모델은 기존의 엔터티 관계도를 이용하여 컨텐츠를 도출토록 하였으며, 프로세스 모델에서는 웹에 구현할 프로세스를 도출하였다. 사용자 인터페이스모델에서는 웹으로 구현할 항해설계 및 멀티미디어 설계를 반영하였다. 본 제안모델은 컨텐츠도출, 컨텐츠를 운용하는 프로세스도출 및 이들의 상관관계를 제시하고 있으며, 이를 통하여 사이트 구조 및 컨텐츠를 상세 정의토록 하였다.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
• /
• 2019.08a
• /
• pp.193-194
• /
• 2019

• The Bulletin of The Korean Astronomical Society
• /
• v.45 no.1
• /
• pp.66.2-66.2
• /
• 2020

We propose a deep learning analysis technique with a convolutional neural network (CNN) to predict the evolutionary track of the Epoch of Reionization (EoR) from the 21-cm differential brightness temperature tomography images. We use 21cmFAST, a fast semi-numerical cosmological 21-cm signal simulator, to produce mock 21-cm maps between z = 6 ~ 13. We then apply two observational effects, such as instrumental noise and limit of (spatial and depth) resolution somewhat suitable for realistic choices of the Square Kilometre Array (SKA), into the 21-cm maps. We design our deep learning model with CNN to predict the sliced-averaged neutral hydrogen fraction from the given 21-cm map. The estimated neutral fraction from our CNN model has great agreement with the true value even after coarsely smoothing with broad beam size and frequency bandwidth and heavily covered by noise with narrow beam size and frequency bandwidth. Our results show that the deep learning analyzing method has the potential to reconstruct the EoR history efficiently from the 21-cm tomography surveys in future.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.19 no.3
• /
• pp.164-173
• /
• 2017

Alternative cropping systems would be one of climate change adaptation options. Suitable areas for a crop could be identified using a climate suitability model. The EcoCrop model has been used to assess climate suitability of crops using monthly climate surfaces, e.g., the digital climate map at high spatial resolution. Still, a high-performance computing approach would be needed for assessment of climate suitability to take into account a complex terrain in Korea, which requires considerably large climate data sets. The objectives of this study were to implement a script for R, which is an open source statistics analysis platform, in order to use the EcoCrop model under a parallel computing environment and to assess climate suitability of maize using digital climate maps at high spatial resolution, e.g., 1 km. The total running time reduced as the number of CPU (Central Processing Unit) core increased although the speedup with increasing number of CPU cores was not linear. For example, the wall clock time for assessing climate suitability index at 1 km spatial resolution reduced by 90% with 16 CPU cores. However, it took about 1.5 time to compute climate suitability index compared with a theoretical time for the given number of CPU. Implementation of climate suitability assessment system based on the MPI (Message Passing Interface) would allow support for the digital climate map at ultra-high spatial resolution, e.g., 30m, which would help site-specific design of cropping system for climate change adaptation.

• Molecules and Cells
• /
• v.25 no.3
• /
• pp.417-427
• /
• 2008

Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.37 no.1
• /
• pp.43-59
• /
• 2017

This study presents the prediction methodology of debris flow occurrence areas using the SINMAP model. Former studies used a single calibration region applying some of the soil test results to predict debris flow occurrence in SINMAP model, which couldn't subdivide the soil properties for the target areas. On the other hands, a multi-calibration region using a detailed soil map and soil strength parameters (c, ${\phi}$) for each soil series to make up for limitation of former studies is proposed. In this process, soils with soil erodibility factor (K) are classified into three types: 1) gravel and gravelly soil. 2) sand and sandy soil, and 3) silt and clay. In addition, T/R estimation method using mean elevation of target area instead of T/R method using actual occurrence time is suggested in this study. The suggested method is applied to Seobyeok-1 ri area, Bonghwa-gun where debris flow occurred. As a result of comparison between two T/R estimation method, both T/R estimations are almost equal. Therefore, the suggested methodologies in this study will contribute to set up the national-wide mitigation plan against debris flow occurrence.

• The Korea Journal of Herbology
• /
• v.32 no.2
• /
• pp.107-114
• /
• 2017

Objectives : Tribulus terrestris $Linn{\acute{e}}$ (Tribuli Fructus; TF) has been used to treat hypochondrium, agalactia, nebula, itching and vitiligo in traditional Korean medicine. In this study, we investigated the effects of TF 30% ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells. Methods : TF extract was prepared by 30% ethanol. RBL-2H3 cells, a rat mast cell line, were treated with TF extract at different concentrations for 1 hr and then stimulated with DNP-IgE/HSA for indicated times. Cell viability was measured by WST-1 assay. The expression of inflammatory cytokines (IL-4, IL-13 and $IFN-{\gamma}$) mRNA was determined by reverse transcriptase-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was determined by Western blot. The nuclear expression of $NF-{\kappa}B$ p65 in the cells was detected by Western blot and immunocytochemistry, respectively. Results : The treatment of TF extract at 0.1 and $0.2mg/m{\ell}$ significantly decreased the expression of IL-4 and IL-13 mRNA in IgE-stimulated RBL-2H3 mast cells, while significantly increased the expression of $IFN-{\gamma}$ mRNA. TF extract treatment was also inhibited the phosphorylation of ERK1/2, p38 and JNK MAPKs in IgE-stimulated RBL-2H3 mast cells in a dose-dependent manner. In addition, TF extract significantly blocked the translocation of $NF-{\kappa}B$ p65 into the nuclear of cells after IgE stimulation. Conclusions : These results indicate that TF extract inhibits inflammatory response in IgE-stimulated mast cells through blocking MAPKs/$NF-{\kappa}B$ pathway. This suggests that TF extract has an anti-inflammatory activity in mast cell activation.