• Journal of the Korean Chemical Society
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• v.28 no.5
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• pp.284-292
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• 1984

This paper aims to predict the substituent and Lewis acid catalysis effect or reactivity on the regioselectivity of (4+2) cycloaddition reaction of the substituted-E-arene-diazocyanides and nitrosobenzenes. Frontier orbital theory (FMO) has been applied to thermal and catalyzed Diels-Alder reaction by means of CNDO/2 and EHT-SPD methods. It has been found that: (1) The above reaction is positive rho(${\rho}$) values in Hammett equation, so it takes normal electron demand reaction, and four-frontier orbitals and Anh methods are identical with experimental major regioisomer.(2) When electron withdrawing radicals are substituted HOMO and LUMO energies of dienophiles are reduced, and the reactivity is increased. (3) The major regioisomer is predicted as B type, as the Lewis acid makes complexes of dienophile, and polaries LUMO coefficients of dienophile in an opposite way. (4) The linear correlation of Hammett is indicated in the graph of stabilized energies(${\Delta}$E) and sigma(${\sigma}$).

• Clinical and Experimental Reproductive Medicine
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• v.22 no.2
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• pp.155-161
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• 1995

The present investigation has been undertaken to elucidate the differentiation mechanism the uterus which is the environment of the embryo development, by demonstrating the role of ovarian steroids hormone in the decidualization of the pseudopregnant rat uterus. To determine the effect of ovarine steroids and artificial stimulation (trauma) on the differenciation of the uterine endometrium and decidualization for implantation, attempt was made to measure concentrations of serum estradiol($E_2$), progesterone($P_4$) and nuclear $P_4$ receptor in the traumatized and non-traumatized uterine tissue of the pseudopregnant rat. The results obtained are as followings : The concentration of serum $E_2$ on day 9(implantation stage) was similar in both of intact pseudopregnant rat(47.63pg/ml) and normal pregnant rat(40.71pg/ml). And among the treated groups, $E_2$ concentration was highest in the $E_2$ treated group in comparision with intact control group(relative value; 73.27%). The concentration of serum $P_4$ was also highest in the $P_4$ treated group(23.12pg/ml). Relative value of $P_4$ treated group in comparision with intact group(24.88pg/ml) was 92.93%. The nuclear $P_4$ receptor levels in the artificial traumatized groups were higher compared with the non-traumatized control groups. This study, therefore, clearly demonstrates that the methods for inducing pseudopregnant (vagina tapping;120/min) and inducing decidualization(oil injection; 0.1ml/uterine horn) appear to be effective, $P_4$ appears to be effective in the differenciation of the uterine endometrial tissue for the implantation process. Concentration of serum $P_4$ seems to be well correlated with the level of the nuclear $P_4$ receptor during the early embryo development. These results seem to be well correlated with ALPase activities in the normal and pseudopregnant rat uterus shown in the previous study.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.430-437
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• 2011

The epidermal recovering-effects of the Cnidium officinale extract after inducing contact-dermatitis by 1-chloro-2,4-dinitrochlorobenzene (DNCB) showed by measuring thickness of skin, identifying IgE amounts in plasma, and activities of anti-oxidant enzymes using histochemical and biochemical methods. By three-time applications of the extract, morphological changes of dermatitis in impaired region were recovered dramatically and shape of skin surface and thickness of epidermis were restored to be normal. Also, lipid content was recovered to the level of normal state. We suggested that extract treatment lowered a hypersensitive reaction by decreasing of IgE level in blood and restored activities of superoxide dismutase and catalase. In conclusion, we proposed that C. officinale extract might be used as natural resources for treating effectively allergic contact-dermatitis.

• Molecules and Cells
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• v.42 no.4
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• pp.333-344
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• 2019

Various genetic and environmental factors are known to be associated with chronic obstructive pulmonary disease (COPD). We identified COPD-related differentially expressed genes (DEGs) using 189 samples accompanying either adenocarcinoma (AC) or squamous cell carcinoma (SC), comprising 91 normal and 98 COPD samples. DEGs were obtained from the intersection of two DEG sets separately identified for AC and SC to exclude the influence of different cancer backgrounds co-occurring with COPD. We also measured patient samples named group 'I', which were unable to be determined as normal or COPD based on alterations in gene expression. The Gene Ontology (GO) analysis revealed significant alterations in the expression of genes categorized with the 'cell adhesion', 'inflammatory response', and 'mitochondrial functions', i.e., well-known functions related to COPD, in samples from patients with COPD. Multi-omics data were subsequently integrated to decipher the upstream regulatory changes linked to the gene expression alterations in COPD. COPD-associated expression quantitative trait loci (eQTLs) were located at the upstream regulatory regions of 96 DEGs. Additionally, 45 previously identified COPD-related miRNAs were predicted to target 66 of the DEGs. The eQTLs and miRNAs might affect the expression of 'respiratory electron transport chain' genes and 'cell proliferation' genes, respectively, while both eQTLs and miRNAs might affect the expression of 'apoptosis' genes. We think that our present study will contribute to our understanding of the molecular etiology of COPD accompanying lung cancer.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.167-173
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• 2023

The purpose of this study was to analyze the accuracy of artificial respiration and chest compressions using CPR mannequins for university students. First, in the number of chest compressions, subjects A, F, H, I, and J showed similar numbers from 60 to 63, respectively, which were much lower than the standard. Subjects B, D, E, and G showed 90 to 91 times, maintaining the normal range. However, C was rather high with 119 times. Second, the depth of chest compression was 58.60mm, and most of them were deeply compressed. Subject C was close to normal at 51mm, and subjects A to J were significantly higher at 55mm to 62mm. The reason seems to be the result of an unstable psychological state with no experience of chest compressions and a lack of self-confidence. Third, in terms of accuracy, subject E showed the lowest accuracy at 12%, and A~J showed 33%~80%. Experiment subject B showed 95% accuracy, which seems to be the result of D accurately recognizing the chest compression point through theoretical training during military service.

• Korean Journal of Radiology
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• v.22 no.8
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• pp.1352-1368
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• 2021

Objective: For an accurate dynamic contrast-enhanced (DCE) MRI analysis, exact baseline T1 mapping is critical. The purpose of this study was to compare the pharmacokinetic parameters of DCE MRI using synthetic MRI with those using fixed baseline T1 values. Materials and Methods: This retrospective study included 102 patients who underwent both DCE and synthetic brain MRI. Two methods were set for the baseline T1: one using the fixed value and the other using the T1 map from synthetic MRI. The volume transfer constant (K^trans), volume of the vascular plasma space (v_p), and the volume of the extravascular extracellular space (v_e) were compared between the two methods. The interclass correlation coefficients and the Bland-Altman method were used to assess the reliability. Results: In normal-appearing frontal white matter (WM), the mean values of K^trans, v_e, and v_p were significantly higher in the fixed value method than in the T1 map method. In the normal-appearing occipital WM, the mean values of v_e and v_p were significantly higher in the fixed value method. In the putamen and head of the caudate nucleus, the mean values of K^trans, v_e, and v_p were significantly lower in the fixed value method. In addition, the T1 map method showed comparable interobserver agreements with the fixed baseline T1 value method. Conclusion: The T1 map method using synthetic MRI may be useful for reflecting individual differences and reliable measurements in clinical applications of DCE MRI.

• Anatomy and Cell Biology
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• v.55 no.1
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• pp.100-108
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• 2022

Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women's MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.1990-1993
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• 2003

In this paper, we propose a technique for automatic spinal deformity detection from $moir\acute{e}$ topographic images. Normally the $moir\acute{e}$ stripes show symmetry as a human body is almost symmetric. According to the progress of the deformity of a spine, asymmetry becomes larger. Numerical representation of the degree of asymmetry is therefore useful in evaluating the deformity. First, displacement of local centroids and difference of gray values are evaluated statistically between the left- and the right-hand side regions of the $moir\acute{e}$ images with respect to the extracted middle line. We classify the moire images into two categories i.e., normal and abnormal cases from the features, employing discriminant analysis. An experiment was performed employing 1,200 $moir\acute{e}$ images and 85% of the images were classified correctly.

• Journal of fish pathology
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• v.6 no.2
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• pp.177-189
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• 1993

1. For the prophylaxis of ceroidosis Vitamin E and C supplemented diet(POV 90.4mEg/kg) was used for flounder culture during 80 days. 2. The supplementation of Vitamin E and C with our tested concentrations on the high POV diet supported almost normal growth of flounder. It is very much comparable with the abnormal growth of flounder fed the diet of high POV. 3. The best prophylactic effect were appeared the high POV diet supplemented with Vitamin E 1mg/g diet and Vitamin C 2IU/g diet. These results were analyzed by histological observation of tissues.

• Biomedical Science Letters
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• v.19 no.4
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• pp.310-314
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• 2013

CYP2E1 in the liver has been studied intensively because it is involved in the metabolic activation of xenobiotics. It is inducible by alcohol, so it has been suspected as the cause of cancer in the stomach and lung. The possible role of CYP2E1 has been suggested strongly as causing tissue damage in mice with renal failure. It was also suspected that 5'-flanking region of CYP2E1 gene might be involved with renal failure. So, we investigated polymorphism of restriction enzyme sites within CYP2E1 gene using the PCR-RFLP analysis. PstI and RsaI sites were located at 5'-flanking region and DraI site was located at intron 6. All three types (W/W, W/S, S/S) were observed for these enzymes although each incidence was somewhat different depending the enzyme sites. W/W was prominent for PstI whereas W/S was markedly high for RsaI. Overall, polymorphic incidence in patients was somewhat higher than normal population. This research should facilitate further investigation of CYP2E1 at genetic level as the direct cause of tissue damage in various organs.