• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.3
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• pp.205-217
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• 2005

Distraction osteogenesis is a technique of lengthening bone including soft tissue by gradual separation of surgically divided bone surfaces. Although the biomechanical, histological, and ultrastructural changes associated with distraction osteogenesis have been widely described, the molecular mechanisms governing the formation of new bone in distracted bone segments remain largely unclear. However, such information has significant clinical implications because it may enable targeted therapeutic manipulations designed to accelerate osseous regeneration. The purpose of this study was to evaluate the expression of TGF-$\beta$1, IGF-I and bFGF in distraction osteogenesis according to different distraction rates in a rabbit's mandible. When twenty-four adult rabbits underwent open osteotomy between the premolar and mental foramen, an external bilateral distraction device was applied. Latency was allowed for five days before distraction. Three different distraction rates were 0.7 mm/day (A, n=8), 1.4 mm/day (B, n=8) and 2.4 mm/day (C, n=8). The distraction device was activated with the same distraction rhythms of twice a day until 4.9 mm (A & B group) and 8.4 mm (C group) length gains was achieved. The animals were sacrificed at postoperative 3, 7, 14 and 28 days. The bony specimens were stained with H&E for histologic examination, and RT-PCR analysis was done for the identification of the expression of TGF-$\beta$1, IGF-I and bFGF. The results obtained from this study were as follows : The 0.7 mm/day and 1.4 mm/day distraction rate groups were shown to improve regenerative bone formation on radiographic and histologic examination. Also, TGF-$\beta$1, IGF-I and bFGF expression increased in the 0.7 mm/day and 1.4 mm/day distraction rate groups. But the 2.4 mm/day distraction rate group specimen was different with adjacent normal bone and hardly expressed of growth factors. These findings suggest that improved new bone formation in the 0.7 mm/day and 1.4 mm/day distraction rates is associated with enhanced expression of TGF-$\beta$1, IGF-I and bFGF by mechanical tension stress. Additionally, the 0.7 mm/day and 1.4 mm/day distraction rate groups were significantly different from the 2.4 mm/day distraction rate group in the expression of growth factors. According to the above results, it seems possible to apply a distraction rate of up to 1.4 mm/day a day in rabbit's mandible. And further studies are needed to evaluate growth factors of TGF-$\beta$1 and IGF-I, which are excellent in expression.

• Journal of the Korea Concrete Institute
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• v.23 no.5
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• pp.617-626
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• 2011

Seven post-tensioned lightweight concrete (LWC) beam specimens were tested under a symmetrical two-point top loading system. The parameters investigated were the amounts of mild longitudinal reinforcement and effective prestressing. The design compressive strength and dry density of the LWC tested were 30 MPa and 1,770 $kg/m^3$, respectively. Similar to post-tensioned normal weight concrete (NWC) beams, the crack propagation and stress increase of the unbonded tendons were significantly affected by the amounts of mild longitudinal reinforcement and effective prestressing. With the increase in the amounts of mild longitudinal reinforcement and effective prestressing, the serviceability and flexural capacity of the beams were enhanced whereas the stress increase in the unbonded tendons decreased. To control the crack width in post-tensioned LWC beams, a minimum amount of mild longitudinal reinforcement specified in ACI 318-08 provision is required. The flexural behavior of post-tensioned LWC beams and stress increase of the unbonded tendons could be rationally predicted by the proposed non-linear two-dimensional analysis. On the other hand, ACI 318-08 flexure provision was too conservative about the post-tensioned LWC beams.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.1
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• pp.108-113
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• 2009

The functional regulator(FR) appliances act to remove the restrictive forces that prevent the normal maturation of the maxilla and mandible. FR appliances are different from other functional jaw orthopedic appliances(e.g., the twin block, bionator, and activator). $Fr{\ddot{a}}nkel$ has based his treatment philosophy on the concept that the capacity to regulate growth residues in the soft tissue environment, and that adequate space must be available for the proper development of the hard tissue. In class II malocclusion with mandibular retrusion, FR-II treatment is not only the change in the postural position of mandible, but also expansion of the dental arches. By balancing the neuromuscular environment, not only can severe malocclusions be treated successfully, but also the tendency toward relapse is minimized because the neural and soft tissue factors associated with the skeletal malocclusion have been addressed as well. We report cases using by FR-II that is applicated in cases of Class II malocclusion without fixed appliance, only FR-II and space supervision.

• Applied Microscopy
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• v.24 no.1
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• pp.28-40
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• 1994

Xerostomia and xerophthalmia are delicate or serous side effects, occuring when the radiotherapy is administered to the head and neck cancer patient. It is known that the cause of the above side effect is radiosensitivity of serous cells. In this study, the ultrastructural features of the parotid glands of the X-irradiated rats were observed. Sprague-Dawley rats weighing 200-250g each were anesthetized with sodium thiopental, and placed on the Mitsubishi linear accelerator. Only the head and neck areas of animals were exposured at the distance of 80cm, within the area of $30X30cm$, in the depth of 1cm, with the speed of 200R/min. Total doses applied were 3,000R or 6,000R depending on the experimental groups. Animals were sacrificed on the 6th hour, 2nd day and 6th day after the irradiation. Parotid glands were fixed in the 2.5% glutaraldehyde-1.5% paraformaldehyde solution, and followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture, and ultrathin sections were cut. Sections were contrasted with the solution of uranyl acetate and lead citrate, and observed with JEM 100 CX-II electron microscope. The results were as follows: 1. Normal parotid acinar cells are two types; the light and the dark acinar cells. The light acinar cell contains dense secretory granules, whereas dark acinar cells contains granules of medium density with some darker spots within them, or other cells contain granules of medium density with darker rims. 2. Six hours after the irradiation, many acinar cells were degenerated showing variable stages of cytolytic bodies, light bodies, or dense degenerations. Within the acinar cell, Golgi apparatus and granular endoplasmic reticula were most severely altered elements. Granules showed more contrasting densities and irregularities. 3. Two days after the irradiation, some cytolytic bodies, and focal lucent degeneration of cytoplasm, and fine granular alteration of cytoplasmic matrix were pronounced. But other elements including secretory granules are rather looked unlatered. 4. Six days after the irradiation, most severe alterations were seen. Many intracellular canaliculi (or secretion figures), quanta of cytoplasm containing secretion antecedants, severely irregular luminal border, and again contrasting density of secretory granules showing tigroid spots or dense rims were noted. And myoepithelial degenerations were observed not uncommonly. 5. Irregular densities of secretory granules were interpreted as abnormal components of protein or carbohydrate portion are synthesized or abnormally metabolized under severe X-irradiation. 6. Myoepithelial degeneration and related alteration of nerve endings, etc., were suggested as the other causes of xerostomia following X-irradiation. 7. It is requested that radiation doses should be arranged, considering in mind not only the sensitivity of acinar cells but also the myoepithelial and neural functions.

• Economic and Environmental Geology
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• v.3 no.2
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• pp.55-73
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• 1970

The district investigated covers the central and southern portions of the Uiryong Quadrangle amounting to $40km^2$ in area and is bounded approximately by geographical coordinates of $128^{\circ}$ 28' $40^{{\prime}{\prime}}{\sim}128^{\circ}$ 24' 25"E in longitude and $35^{\circ}10{\prime}{\sim}35^{\circ}14^{\prime}06^{{\prime}{\prime}}N$ in latitude. The purpose of this investigation was to provide basic information in drawing up a comprehensive development plan of the copper ore deposits known to exist in the HamanKumbuk district with special emphasis given to the ascertainment of geological and paragenetic characteristics. The area consists chiefly of shale, sandy shale and chert, all belong to Kyongsang System of Cretaceous age. Intruded into these rocks are andesite, granodiorite, basic dikes, and acidic dikes. The mineralization which took place in the area, consists of mostly fissure-filling vein deposits, numbering several tens, with varying magnitudes. The fissures and shear zones created in rocks, such as chert and granodiorite, hosted the deposition of mineralizing vapors and/or hydrothermal solutions along their openings. The strike lengths of these veins vary from 50 to 600 meters in extension and 0.1 to 3 meters in width. Although the degree of fluctuation in width is great, it averages 0.3m. The stuctural patterns, which apparently affected the deposition of veins, are fissure patterns, trend NS to $N30^{\circ}W$, and steep-pitching tension fractures as well as normal fault pattern. Ore minerals associated with vein matters are primarily chalcopyrite and small amounts of scheelite, cobaltiferous arsenopyrite, and gold and silver intimately associated with sulphide minerals. Associated with these ore mineral are pyrite, pyrrhotite, magnetite, specularite and arsenopyrite. Gangue minerals noted are quartz, calcite, chlorite, tourmaline and hornblende. In terms of the compositions of associated minerals, the vein deposits in the district could be grouped under the following four categories: 1. Pyrrhoitite, Arsenopyrite, Gold and Silver Bearing Copper Vein (Type I) 2. Calcite-Scheelite-Copper Vein (Type II) 3. Magnetite-Pyrite-Copper Vein (Type III) 4. Tourmaline Copper Vein (Type IV) Of the four types, the first and the fourth are presently yielding relatively higher grades: of copper ores and concentrates. The estimated ore reserves total some 222,000 metric tons with the following breakdown in terms of metal contents: Name of Mines Au(g/t) Ag(g/t) Cu(%) Reserves(M/T) Kunbuk 15.92 78.69 6,074 60.498 Cheil Kunbuk - - 1.040 60,847 Haman - - 2.688 101,204 222,549 As rehabilitation of old workings and/or exploration of veins at depth proceed, additional estimation of ore reserves may become apparent and necessary. With regard to the problem of beneficiation and upgrading of low-grade ores in the district, it would be advisable to make decisions on location, treating capacity and mill flowsheet after sufficient amount of exploration is completed as suggested in the report.

• Journal of Embryo Transfer
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• v.25 no.3
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• pp.149-154
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• 2010

The objective of this study was to investigate the effects of abnormal ovarian cycles after superovulation treatment of Hanwoo donors. Thirty six, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg $PGF_2{\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received $100\;{\mu}g$ GnRH at the time of Ind insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The cows were considered to have resumed ovarian cyclicity on the day of ovulation if followed by regular ovarian cycles. 50.0 percentage of the cows (18/36) had normal resumption of ovarian cyclicity (resumption within 40 days after superovulation), and 50.0% (18/36) had delayed resumption(resumption did not occur until>40 days after superovulation). Delayed resumption Type II (first ovulation did not occur until $\geq$ 40 days after superovulation, i.e. delayed first ovulation 33.3%) were the most common types of delayed resumptions. The mean numbers of total ova from < 10 and 10$\leq$ of corpora lutea (CL) was 7.3 and 13.9, respectively. The number of transferable embryos differed between < 10 and 10$\leq$ CL was 4.2 and 5.1, respectively. 11.1 percentage of the cows (4/36) did not resumption their ovarian cyclicity until 60 days after superovulation treatment.

• Journal of fish pathology
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• v.24 no.1
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• pp.11-18
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• 2011

This study was aimed to investigate the pathophysiological changes of olive flounder, Paralichthys olivaceus suffering from emaciation. A plasma osmolality was higher in the emaciated and control flounders than that of normal teleost, suggesting osmoregulatory failure in both of them. Also, the control in the same stock with emaciated flounder seem to be classified into a primary degree of emaciation. According to microscopic observations, the inflammatory responses were observed in the submucosal layer of anterior intestine, although the some of mucosal intestinal epithelium still remained. It was suggested that the pathological changes of the anterior part give rise to malabsorption of nutrients through the mucosa. In the posterior intestine and rectum, the mucosal epithelium were almostly sloughed off and severe inflammatory responses were observed in the submucosa. Immunoreaction for NKCC was not detected in the mucosal epithelial cells in intestine because of sloughing of epithelium. These changes would lead to functional disorder in the intestine, such as malabsorption of nutrients and osmoregulatory failure. Also important is to investigate the recovery phase.

• Journal of Pharmacopuncture
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• v.9 no.1
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• pp.5-20
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• 2006

Objectives : This study was carried out to identified the effects of distilled cultivated wild ginseng pharmacopuncture to the obesity. Methods : Cultivated wild ginseng pharmacopuncture was administered on the points of chung-wan(CV12), $Ch'{\breve{o}}nch'u$(ST25), and Chok-samni(ST36) on lowering lipid and oxidative capacity in biochemical and molecular biological aspects were investigated in obese rats fed with high fat diet. Results : 1. The contents of plasma ${\beta}-lipoprotein$ showed a tendency to decrease in the pharmacopuncture groups compared to the control group. In the pharmacopuncture groups, the values of ST25 and ST36 pharmacopuncture groups showed lower value. 2. The contents of plasma free fatty acids showed a tendency to decrease in pharmacopuncture groups compared to the control group. However, in the pharmacopuncture groups, the values were not significantly different. 3. Plasma triglyceride and glucose showed lower value in the ST25 pharmacopuncture groups compared with the other groups. 4. The activity of AST showed a tendency to decrease in the pharmacopuncture groups. However, the activity of ALT was not significantly different in all the treatment groups. 5. Plasma total cholesterol and LDL-cholesterol showed lower value in the ST25 and ST36 pharmacopuncture groups and HDL-cholesterol showed higher value in the CV12 pharmacopuncture groups than that of the other treatment groups. 6. Liver total cholesterol values didn't show significant difference in all the treatment groups, and triglyceride showed lower value in the pharmacopuncture groups. 7. The contents of plasma TEARS showed lower value in the ST25 pharmacopuncture group and contents of liver TBARS showed a tendency to decrease in the pharmacopuncture groups. However these values didn't show significant difference in the pharmaco puncture groups. 8. Liver super oxide dismutase activity showed higher value in the ST25 and ST36 pharmacopuncture groups, and the value of liver glutathione peroxidase and catalase activity showed a tendency to increase in the pharmacopuncture groups. However, these values showed no significant difference in the pharmacopuncture groups. 9. Expression of apo-B and E mRNA in liver cells was lower in the ST25 pharmacopuncture group than that of the other treatment groups. However, expression of $TNF-{\alpha}$ and leptin mRNA in adipose cell showed no difference among all the treatment groups. 10. ST25 pharmacopuncture group showed a good histological character of liver. It showed similar to that of normal group. However other treatment groups and control group showed slight vasodilation and slight fat accumulation. Conclusion : These results indicate that distilled cultivated wild ginseng pharmacopuncture suppressed adipose tissue mass and lipid peroxidation, and increased antioxidant capacity.

• Radiation Oncology Journal
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• v.37 no.4
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• pp.259-264
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• 2019

Purpose: Accurate localization of the lumpectomy cavity during accelerated partial breast radiation (APBR) is essential for daily setup to ensure the prescribed dose encompasses the target and avoids unnecessary irradiation to surrounding normal tissues. Three-dimensional ultrasound (3D-US) allows direct visualization of the lumpectomy cavity without additional radiation exposure. The purpose of this study was to evaluate the feasibility of 3D-US in daily target localization for APBR. Materials and methods: Forty-seven patients with stage I breast cancer who underwent breast conserving surgery were treated with a 2-week course of APBR. Patients with visible lumpectomy cavities on high quality 3D-US images were included in this analysis. Prior to each treatment, X-ray and 3D-US images were acquired and compared to images from simulation to confirm accurate position and determine shifts. Volume change of the lumpectomy cavity was determined daily with 3D-US. Results: A total of 118 images of each modality from 12 eligible patients were analyzed. The average change in cavity volume was 7.8% (range, -24.1% to 14.4%) on 3D-US from simulation to the end-of-treatment. Based on 3D-US, significantly larger shifts were necessary compared to portal films in all three dimensions: anterior/posterior (p = 7E-11), left/right (p = 0.002), and superior/inferior (p = 0.004). Conclusion: Given that the lumpectomy cavity is not directly visible via X-ray images, accurate positioning may not be fully achieved by X-ray images. Therefore, when the lumpectomy cavity is visible on US, 3D-US can be considered as an alternative to X-ray imaging during daily positioning for selected patients treated with APBR, thus avoiding additional exposure to ionizing radiation.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.549-554
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• 2011

FK506 is a widespread immunosuppressive drug after liver transplantion in patients with advanced-stage hepatocellular carcinoma. Dexamethasone is frequently used as co-treatment in cytotoxic cancer therapy, e.g. to prevent nausea, to protect normal tissue or for other reasons. Our aim was to investigate antitumor effects of FK506 in Hep3B cells, one of differentiated human hepatocellular carcinoma cell lines and inhibitory effects of dexamethsone on FK506- induced antitumor effects. Cell injury was evaluated by biochemical assays as cell viability, lactate dehydrogenase (LDH) and reactive oxygen species (ROS) in Hep3B cells. Intracellular calcium concentration ([$Ca^{2+}$]i) and the level of activation of the c-Jun-N-terminal kinase (JNK) and the Bax protein in cultured Hep3B cells was measured. Exposure of 0.1 ${\mu}M$ FK506 to Hep3B cells led to cell death accompanied by a decrease in cell viability and an increase in LDH, ROS and [$Ca^{2+}$]i. FK506 induced an increase in activity of Bax and JNK protein but inhibited the activity of Bcl-2 protein. Treatment of dexamethsone, per se, had no effects on cell viability, LDH and ROS. However, co-treatment of FK506 and dexamethasone diminished the FK506-induced LDH release, ROS generation and JNK activation. These results demonstrate that FK506 has antitumor effect in Hep3B cells but the combination of FK506 and dexamethasone antagonizes the FK506-induced antitumor effects.