• Journal of Pharmaceutical Investigation
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• v.35 no.1
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• pp.45-50
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• 2005

The purpose of the present study was designed to evaluate the bioequivalence of two ofloxacin tablets, Tarivid (Jeil Pharm. Co., Ltd.) and Favid (ILHWA Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four normal male volunteers, $23.67{\pm}3.12$ year in age and $68.50{\pm}7.23$ kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After four tablets containing 100 mg of ofloxacin were orally administered, blood was taken at predetermined time intervals and concentrations of ofloxacin in plasma were determined using HPLC. Pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$ and $C_{max}$ and untransformed $T_{max}$. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transformed data were acceptance range of log 0.8 to log 1.25 (e.g., $log0.94{\sim}log1.04\;and\;log0.90{\sim}log1.07\;for\;AUC_t\;and\;C_{max}$, respectively). The major parameters, $AUC_t$, and $C_{max}$, met the criteria of KDFA for bioequivalence indicating that Favid tablet is bioequivalent to Tarivid tablet.

• Journal of Pharmaceutical Investigation
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• v.35 no.3
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• pp.187-192
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• 2005

The purpose of the present study was designed to evaluate the bioequivalence of two benidipine hydrochloride tablets, Codipine (Youngjin Pharm. Co., Ltd.) and Benipine (Myungmoon Pharm. Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four normal male volunteers, $23.00{\pm}1.82$ year in age and $70.08{\pm}9.59$ kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After two tablets containing 4 mg of benidipine hydrochloride were orally administered, blood was taken at predetermined time intervals and concentrations of benidipine in plasma were determined using LC-MS/MS. Pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$, and $C_{max}$. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transformed data were acceptance range of log0.8 to log1.25 $(e.g., \;log1.04{\sim}log1.24\;and\;log0.91{\sim}log1.09$ for $AUC_t$, and $C_{max}$ respectively). The major parameters, $AUC_t$ and $C_{max}$, met the criteria of KDFA for bioequivalence indicating that Benipine tablet is bioequivalent to Codipine tablet.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.4
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• pp.474-482
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• 2015

This study aims to investigate the patterns and incidence rates of morphological deformities during artificial fingerling production of blackhead seabream (BSB, Acanthopagrus schlegeli) juveniles by visual and soft X-ray observations of BSB juveniles (n=881) collected from four industrial hatcheries. Using both methods, we observed seven types of external deformity ($11.2{\pm}4.4%$) and eight types of skeletal deformity ($53.4{\pm}5.1%$). Lordosis occurred in all hatcheries with the highest frequency and the mean frequency was $13.1{\pm}4.4%$ (24.5% in total abnormal) by soft X-ray. Although they had a similar standard length (SL), juveniles with lordosis had a significantly lower condition factor and body weight than normally developed juveniles (P<0.05); normal distribution curves of histogram of body proportions, i.e., body height (BH)/head length (HL) and trunk (=SL-HL)/BH, indicated differences between normally developed juveniles and those with lordosis. In addition, 59% of juveniles with lordosis had vertebral curvature of less than $20^{\circ}$, generally at the $17^{th}$ vertebrae. It is expected that the above results will be used as basic data necessary for determining the cause of lordosis and for developing techniques to prevent this symptom in the fingering production industry of BSB juveniles.

• Wind and Structures
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• v.5 no.2_3_4
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• pp.177-192
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• 2002

Computation solutions for the flow around a cube, which were generated as part of the Computational Wind Engineering 2000 Conference Competition, are compared with full-scale measurements. The three solutions shown all use the RANS approach to predict mean flow fields. The major differences appear to be related to the use of the standard $k-{\varepsilon}$, the MMK $k-{\varepsilon}$ and the RNG $k-{\varepsilon}$ turbulence models. The inlet conditions chosen by the three modellers illustrate one of the dilemmas faced in computational wind engineering. While all modeller matched the inlet velocity profile to the full-scale profile, only one of the modellers chose to match the full-scale turbulence data. This approach led to a boundary layer that was not in equilibrium. The approach taken by the other modeller was to specify lower inlet turbulent kinetic energy level, which are more consistent with the turbulence models chosen and lead to a homogeneous boundary layer. For the $0^{\circ}$ case, wind normal to one face of the cube, it is shown that the RNG solution is closest to the full-scale data. This result appears to be associated with the RNG solution showing the correct flow separation and reattachment on the roof. The other solutions show either excessive separation (MMK) or no separation at all (K-E). For the $45^{\circ}$ case the three solutions are fairly similar. None of them correctly predicting the high suctions along the windward edges of the roof. In general the velocity components are more accurately predicted than the pressures. However in all cases the turbulence levels are poorly matched, with all of the solutions failing to match the high turbulence levels measured around the edges of separated flows. Although all of the computational solutions have deficiencies, the variability of results is shown to be similar to that which has been obtained with a similar comparative wind tunnel study. This suggests that the computational solutions are only slightly less reliable than the wind tunnel.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.291-298
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• 2008

For many years beak trimming has been a controversial subject, particularly since the 1980's when the practice came under close scrutiny by animal welfare groups. In Australia it is considered an essential practice, averting losses of AUD$17.5m annually by reducing mortality from cannibalism. While mortality in flocks from cannibalism can be reduced from 25% of the flock to virtually nil, the beak trimming procedure is considered traumatic for the bird. This study examined if chronic pain in the beak was evident in birds 10, 20 and 60 weeks after being trimmed at hatch and in another group of birds, 8 and 52 weeks after being re-trimmed at 14 weeks. Chronic pain was assessed by measuring pecking behaviour and beak sensitivity responses. Pecking behaviour studies completed after beak trimming and re-trimming showed no evidence to indicate that birds were suffering severe chronic pain in the beak. Beak trimmed pullets pecked more at the cage and had more toe pecks, yet overall pecks made at the feed and the environment were no different than untrimmed controls. While the beak sensitivity studies provided evidence that the beak of birds trimmed at hatch and also re-trimmed at 14 weeks may be more sensitive there was no evidence that re-trimming resulted in a more sensitive beak than birds trimmed at hatch only. These studies have shown that birds which are beak trimmed and re-trimmed return to apparently normal feeding and pecking behaviour in the long term. However, there was limited evidence that beaks of trimmed birds have an altered threshold to potentially painful stimuli.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1589-1598
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• 2015

In this work, the anti-aging skin effects of bacteriochlorophyll a isolated from Rhodobacter sphaeroides are first reported, with notably low cytotoxicity in the range of 1% to 14% in adding 0.00078 (% (w/w)) of the extracts, compared with the normal growth of both human dermal fibroblast and keratinocyte cells without any treatment as a control. The highest production of procollagen from human fibroblast cells (CCD-986sk) was observed as 221.7 ng/ml with 0.001 (% (w/w)) of bacteriochlorophyll a, whereas 150 and 200 ng/ml of procollagen production resulted from addition of 0.001 (% (w/w)) of the photosynthetic bacteria. The bacteriochlorophyll-a-induced TNF-α production increased to 63.8%, which was lower secretion from HaCaT cells than that from addition of 0.00005 (% (w/w)) of bacteriochlorophyll a. Additionally, bacteriochlorophyll a upregulated the expression of genes related to skin anti-aging (i.e., keratin 10, involucrin, transglutaminase-1, and MMPs), by up to 4-15 times those of the control. However, crude extracts from R. sphaeroides did not enhance the expression level of these genes. Bacteriochlorophyll a showed higher antioxidant activity of 63.8% in DPPH free radical scavenging than those of water, ethanol, and 70% ethanol extracts (14.0%, 57.2%, and 12.6%, respectively). It was also shown that the high antioxidant activity could be attributed to the skin anti-aging effect of bacteriochlorophyll a, although R. sphaeroides itself would not exhibit significant anti-aging activities.

• Journal of Microbiology and Biotechnology
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• v.25 no.12
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• pp.2049-2057
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• 2015

Various salt concentrations (1.0%, 1.3%, 1.6%, 1.9%, and 2.1% labeled as sample A, B, C, D, and E, respectively) were investigated for microbial diversity, identification of Lactic Acid Bacteria (LAB) in salted kimchi cabbage, prepared under laboratory conditions. These samples were stored at 4°C for 5 weeks in proper aluminum-metalized pouch packaging with calcium hydroxide gas absorber. A culture-independent method known as polymerase chain reaction - denaturing gradient gel electrophoresis was carried out to identify LAB distributions among various salt concentration samples that had identified 2 Weissella (W. confusa and W. soli), 1 Lactobacillus (Lb. sakei), and 3 Leuconostoc (Lc. mesenteroides, Lc. lactis, and Lc. gelidum) in the overall kimchi samples. The pH, titratable acidity, viable cell counts, and coliform counts were not affected by salt variations. In order to assess sensory acceptance, the conducted sensory evaluation using a 9-point hedonic scale had revealed that samples with 1.3% salt concentration (lower than the manufacturer's regular salt concentration) was more preferred, indicating that the use of 1.3% salt concentration was acceptable in normal kimchi fermentation for its quality and safety. Despite similarities in pH, titratable acidity, viable cell counts, coliform counts, and LAB distributions among the various salt concentrations of kimchi samples, the sample with 1.3% salt concentration was shown to be the most preferred, indicating that this salt concentration was suitable in kimchi production in order to reduce salt intake through kimchi consumptions.

• Ocean and Polar Research
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• v.25 no.1
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• pp.31-40
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• 2003

In August-September 2001, 15 samples of bottom sediments were collected in the inner, middle and open parts of Amursky Bay near Vladivostok, Russia, and barfin plaice Pleuronectes pinnifasciatus was sampled from the inner and the middle locations of the bay. In the sediments from all three sites elevated concentrations of several heavy metals, i.e. Zn ($102-115{\mu}/g$ dry weight), Ni $(70-73{\mu}g/g)$ and Cu $(27-35{\mu}g/g)$ were discovered. The contents of oil hydrocarbons were very close to or slightly higher than the maximal normal environmental background level, $100{\mu}g/g$ dry weight. The sediments contained negligible amounts of hexachlorocyclohexane, while DDT concentrations were quite high (1.7-16.3ng/g dry weight). Generally, there were no substantial differences in the pollution levels of the locations studied and our results resembled those reported for Amursky Bay in the 1990s. Surprisingly, in 2001 'fiesh' DDT comprised 70-85% of the total DDT content in sediment from all the locations studied. In fish liver total DDTs concentrations were 212.8 and 122.54 ng/g wet weight for the inner and the middle locations, respectively, and 'fresh' DDT comprised 35 and 64% of DDTs, respectively. These results provide evidence of recent input of DDT from an unknown source into the ecosystem of Amursky Bay. Histopathological changes revealed in the plaice liver (vacuolization of hepatocytes, coagulative necrosis of hepatocytes, inflammatory reaction, and necrosis of epithelial cells of bile ducts) are probably connected with an intensive metabolism of DDT in the fish organism. No histological and histomorphometric differences were found in the state of the interrenal tissue. Similar condition of the liver and the interrenal tissue in barfin plaice sampled from the inner and the middle locations of Amursky Bay may be explained by the absence of great differences in the pollution levels of these sites.

• Journal of Nutrition and Health
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• v.33 no.4
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• pp.438-453
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• 2000

The purposes of this study were to estimate nutritional intakes of the long-lived elderly and to obtain the data for establishing dietary guidelines that may be recommended for the general population for the sake of longevity. The subjects of the study were 300 elederly people of age over 85 years living in Kyungpook Sung-Ju area who had no problem in daily living. Four times of food consumption survey were carried out seasonally by the repeated 24-hr recall method for one year. Mean daily energy intakes and RDA percentage of energy intakes of the male and female subjects were estimated as 1222 kcal(67.9%) and 1047 kcal(65.4%) respectively. Mean daily intakes of nutrients were estimated as 38.3g for protein, 287mg for calcium, 5.8mg for iron, 314R.E. for vitamin A, 0.6mg for vitamin B1 and 0.43mg for vitamin B2 The mean RDA percentages of nutrients intake were 64.4% and 59.8% for protein in male and female, 39.8% for Ca, 48.3% for Fe, 44.9% for vitamin A, 60% for vitamin B1, and 35.8% for B2. The average PFC ratio of energy-yielding nutrients throughout the year in male and female were 15.1 : 15.2 : 69.7 and 13.8 : 13.2 : 73.0 respectively. The mean daily intakes of energy and most of nutreints were significantly high in winter season. The contribution of plant food sources to nutrient intakes were over 60% for protein and fat, 50% for calcium, and 70% for iron. Long-lived elderly people an Sung-Ju, Kyungpook showed considerably smaller physiques compared to the avergae Korean elderly, however their average BMI fell in normal range. The subjects were consuming much less energy and nutrients compared to the present Korean RDA for the elderly over age 75. The subject showed relatively good health state in spite of low intakes of energy and nutrients. Therefore it seems to be necessary to establish a set of new RDA for the elderly over age 8.5.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.9-14
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• 1984

To study the correlation of the net drag force, net height, and towing speed, three kinds of similiar size model trawl nets were experimented in the still watertank ($60m{\times}4m{\times}3m$). The scale ratios of model nets, 2 seam, 4 seam, and 6 scam net were 1/31.3, 1/20.0, and 1/44.4 respectively, The maximum streched circumferences of the bag net were same length, i. e. 140cm. Net drags were propotional to the $1.75{\sim}1.98th$ order of towing speed and showed similar result as Koyama's net drag equation. Net heights were propotional to the $-0.85{\sim}-0.72th$ order of towing speed. It could observe that the towing nets showed normal shape in $3.0{\sim}3.5$ knot full scale towing speed but bad shape below $1.0{\sim}1.5$ knot. And it showed tendency to lift the bag net and codend with increasing speed.