• 대한임상검사과학회지
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• 제38권3호
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• pp.179-183
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• 2006

Alzheimer's disease (AD) is characterised neuropathologically by the accumulation of neuritic plaques and neurofibrillary tangles as well as by cerebrovascular amyloid deposition and neuronal cell loss. Current advances have shown the apolipoproteinE-epsilon 4 (ApoE4) allele to be highly associated with late-onset familial and sporadic Alzheimer's disease (AD) in Western populations. The association of ApoE allele frequencies and dementia remains unknown in populations from many countries. We recently initiated a project to examine ApoE frequencies in non-demented healthy Koreans. Genomic DNA in hair root from a thousand persons was collected and ApoE gene type was investigated with the methods of polymerase chain reaction (PCR) and restriction fragment length polymorphism. A group of a thousand non-demented Koreans over the age of 40 years were found to be positive in 15.7% of the cases for ApoE4. AD and ApoE4 were closely related. ApoE epsilon 4 was a dangerous factor of AD and ApoE 4 allele made a contribution to the heterogenicity of AD.

• 한국가금학회지
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• 제50권1호
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• pp.63-72
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• 2023

This study was conducted to investigate the effect of vitamin E and nanoparticle-sized vitamin E (NVE) in a broiler diet on growth performance, blood profiles, and meat quality. A total of 45 one-day-old Arbor Acres broilers (initial body weight of 37.00 ± 0.41 g) were used in this experiment for 28 days. All broilers were randomly allocated into three dietary treatments in a randomized complete block design. The dietary treatments were as follows: a basal diet (CON), a basal diet supplementing with 380 mg/kg of vitamin E (T1), and a basal diet supplementing with 380 mg/kg of NVE (T2). Each treatment had five replicates with three birds per cage. On days 0-7, the T1 and T2 groups significantly increased (P<0.05) body weight gain compared to the CON group. Also, the T1 and T2 groups significantly increased (P<0.05) vitamin E content in blood and breast meat compared to the CON group. In shearing force, the T2 group showed a lower tendency (P=0.070) than the CON group. The T1 group showed a higher tendency (P=0.086) in the b* (yellowness) value than the T2 group. On day 7 after the end of the experiment, the T2 group significantly decreased (P<0.05) TBA values compared to the CON group. In conclusion, supplementation with vitamin E or NVE can improve broiler growth performance in the starter period, reduce TBA value through the antioxidant action of vitamin E, prevent lipid oxidation, and improve shelf life.

• Journal of Astronomy and Space Sciences
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• 제38권2호
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• pp.135-143
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• 2021

We report, for the first time, the afternoon (i.e., from noon to sunset time) observations of the northern mid-latitude E-region field-aligned irregularities (FAIs) made by the very high frequency (VHF) coherent backscatter radar operated continuously since 29 December 2009 at Daejeon (36.18°N, 127.14°E, 26.7°N dip latitude) in South Korea. We present the statistical characteristics of the mid-latitude afternoon E-region FAIs based on the continuous radar observations. Echo signal-to-noise ratio (SNR) of the afternoon E-region FAIs is found to be as high as 35 dB, mostly occurring around 100-135 km altitudes. Most spectral widths of the afternoon echoes are close to zero, indicating that the irregularities during the afternoon time are not related to turbulent plasma motions. The occurrence of afternoon E-regional FAI is observed with significant seasonal variation, with a maximum in summer and a minimum in winter. Furthermore, to investigate the afternoon E-region FAIs-Sporadic E (E_s) relationship, the FAIs have also been compared with E_s parameters based on observations made from an ionosonde located at Icheon (37.14°N, 127.54°E, 27.7°N dip latitude), which is 100 km north of Daejeon. The virtual height of E_s (h'E_s) is mainly in the height range of 105 km to 110 km, which is 5 km to 10 km greater than the bottom of the FAI. There is no relationship between the FAI SNR and the highest frequencies (f_tE_s) (or blanket frequencies (f_bE_s)). SNR of FAIs, however, is found to be related well with (f_tE_s-f_bE_s).

• 대한바이러스학회지
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• 제28권1호
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• pp.53-62
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• 1998

Human papillomavirus (HPV) 16, E7 proteins derived from the prototype (Bac73) and natural variant (Bac101) E7 open reading frame were produced in Sf9 insect cells. The variant E7 gene occurred naturally by substitution mutation at the position of 88 nucleotide, resulting serine instead of asparagine. Using E7 specific monoclonal antibody (VD6), both E7 proteins were identified in recombinant baculovirus infected SF9 cells. Radiolabelling and immunoprecipitation analysis revealed that both E7 proteins were phosphoproteins. Immunostaining result showed that E7 proteins were mainly localized in the cytoplasm. Nuclear form of E7 proteins was also detected after a sequential fractionation procedure for removing chromatin structure. Considering that the VD6 recognition site in E7 protein is located within 10 amino acid at the N-terminus, this region appears to be blocked by the nuclear component. Western blot analysis revealed that nuclear form was more abundant than cytoplasmic E7 proteins. Time course immunostaining showed that the primary location of E7 protein was the nucleus and exported to the cytoplasm as proteins were accumulated. These events occurred similarly in both Bac73 and Bac101 infected Sf9 cells, suggesting that these two proteins may have similar biological functions.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(B)
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• pp.276-279
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• 2011

컴퓨터 기술이 발전함에 따라 텍스트, 이미지, 동영상 등의 여러 시청각 매체를 한 곳으로 모아 놓은 e-book이 등장하였다. 본 논문에서는 컴퓨터 교과를 위한 실습 중심형 e-book 컨텐츠를 설계하고 구현한다. 이를 위해, 교수 매체의 개념과 종류, 교육용 e-book의 특징 등을 살펴본다. 다음으로, 일반계 고등학교의 "정보사회와 컴퓨터" 교과를 전문계 교과와 비교하여 e-book 구현에 적합한 단원을 선택한 후, ADDIE 모형에 입각하여 e-book 컨텐츠를 개발한다. 또한, 구현한 e-book 컨텐츠를 통해 교수 학습 지도안을 제시하고 e-book 컨텐츠 활용방안을 제안한다.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.199-211
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• 2021

Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species. However, in equidaes, eCG exhibits only LH-like activity. To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit⁵⁶ N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant. We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells. We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased. The EC₅₀ levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG. The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/10⁴ cells). Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR. These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.

• 한국항공우주학회지
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• 제36권5호
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• pp.428-437
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• 2008

e-AIRS는 e-Aerospace Integrated Research System의 약어로, e-Science 인프라를 기반으로 한 항공우주 통합연구 환경을 말한다. 이러한 항공우주 통합연구 환경구축을 위한 첫 단계로, 공력분야의 통합연구를 위한 환경을 구축하였으며, 현재 e-AIRS 시스템을 통하여, 수치적 및 실험적 연구를 모두 수행할 수 있다. 사용자는 e-AIRS 웹 포털을 통하여 수치해석 연구의 전 과정을 수행할 수 있으며, 풍동실험의 요청 및 수치해석결과와 실험결과의 비교 연구, 그리고 협업 시스템을 이용한 연구자간의 협력 연구를 수행할 수 있다. 본 논문에서는 이러한 e-AIRS 시스템의 구조와 기능 및 활용에 대해 설명한다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3843-3847
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• 2015

Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.

• Asia pacific journal of information systems
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• 제21권1호
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• pp.49-80
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• 2011

E-government needs to be successfully implemented and assimilated into organizations to take advantage of its potential values and benefits for organizations. This study examines factors for e-government assimilation in Indonesia and employs the TOE (Technology-Organization-Environment) framework to develop a theoretical model to explain e-government assimilation. It also investigates how organizational type (central vs. local) plays a role in the assimilation of e-government. One hundred eighteen respondents from the central and local governments in Indonesia participated in the survey and an in-depth analysis based on partial least squares (PLS) was carried out. The results show that ICT infrastructure has the strongest significant relationship with e-government assimilation, Top management support, regulatory environment, ICT expertise, and competitive environment are also significant factors to explain e-government assimilation in Indonesia. Central and local governments Significantly differ in terms of e-government assimilation, so organizational type can be a moderator in the process of e-government assimilation. These findings present the efficacy of the proposed model for analyzing e-government assimilation and contribute additional insights for academia as well as practitioners and policy makers.

• 한국과학교육학회지
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• 제29권6호
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• pp.626-638
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• 2009

본 연구에서는 R&E 프로그램에 참여한 270명의 학생을 대상으로 R&E의 목적, 주제 선정 과정, R&E의 장단점에 관하여 설문 조사를 실시하였고, 4명의 학생을 대상으로 심층인터뷰를 실시하였다. R&E의 목적과 장점에 대하여 학생들은 '예비 과학자로의 자질 함양, '연구 기회 제공', 탐구 수행 능력 신장'으로 인식하였으며, 단점으로는 '연구에 소비된 시간', '타율적 참여', '연구 내용의 어려움' 등을 언급하였다. R&E 프로그램은 정해진 주제 제시된 실험과정 문제 발생 시 전문가의 도움으로 진행되는 기술적 도제과정이 대부분이었으며, 창의적 문제해결능력 신장보다는 새로운 실험 장비나 첨단 기술을 접하는 기회 제공 차원이 큰 것으로 나타났다. R&E 프로그램에서 멘토의 역할이 중요하며, 멘토의 역할은 학생들의 생산적 참여 정도와 과학 및 과학자의 인식에 큰 영향을 주었다.