• Title/Summary/Keyword: Duplex structure

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NMR hydrogen exchange study of miR156:miR156* duplexes

  • Kim, Na-Hyun;Choi, Seo-Ree;Jin, Ho-Seong;Seo, Yeo-Jin;Lee, Joon-Hwa
    • Journal of the Korean Magnetic Resonance Society
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    • v.23 no.3
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    • pp.61-66
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    • 2019
  • RNAs exhibit distinct structural and dynamic features required for proper function. The hydrogen-bonded imino protons of RNAs are a probe of the conformational transition and dynamic feature. MicroRNAs originate from primary transcripts containing hairpin structures. The levels of mature miR156 influence the flowering time of plants. To understand the molecular mechanism of biological function of $miR156:miR156^*$ duplex, we performed hydrogen exchange study on the model RNAs mimicking two phenotypes of $miR156:miR156^*$, $miR156:miR156^*$ (m-miR156a) and $miR156:miR156^*$ (m-miR156g) duplexes. This study found that the internal bulge of m-miR156a destabilized the neighboring base-pairs, whereas the bulge structure of m-miR156g did not affect the thermal stabilities of the neighboring base-pairs.

Effect of bolt preloading on rotational stiffness of stainless steel end-plate connections

  • Yuchen Song;Brian Uy
    • Steel and Composite Structures
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    • v.48 no.5
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    • pp.547-564
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    • 2023
  • This study investigates the effect of bolt preloading on the rotational stiffness of stainless steel end-plate connections. An experimental programme incorporating 11 full-scale joint specimens are carried out comparing the behaviours of fully pre-tensioned (PT) and snug-tightened (ST) flush/extended end-plate connections, made of austenitic or lean duplex stainless steels. It is observed from the tests that the presence of bolt preloading leads to a significant increase in the rotational stiffness. A parallel finite element analysis (FEA) validated against the test results demonstrates that the geometric imperfection of end-plate has a strong influence on the moment-rotation response of preloaded end-plate connections, which is crucial to explain the observed "two-stage" behaviour of these connections. Based on the data obtained from the tests and FE parametric study, the performance of the Eurocode 3 predictive model is evaluated, which exhibits a significant deviation in predicting the rotational stiffness of stainless steel end-plate connections. A modified bi-linear model, which incorporates three key properties, is therefore proposed to enable a better prediction. Finally, the effect of bolt preloading is demonstrated at the system (structure) level considering the serviceability of semi-continuous stainless steel beams with end-plate connections.

Impedance investigation of the surface film formed on aluminum alloy exposed to nuclear reactor emergency core coolant

  • Junlin Huang;Derek Lister;Xiaoliang Zhu;Shunsuke Uchida;Qinglan Xu
    • Nuclear Engineering and Technology
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    • v.55 no.4
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    • pp.1518-1527
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    • 2023
  • A method was proposed for in-situ evaluating the thickness and resistivity of the oxide/hydroxide film formed on the surface of aluminum alloy exposed to sump water formed in the containment after a loss-of-coolant accident. The evaluation entailed fitting a model for the film impedance, which has film thickness and other variables describing the resistivity profile of the film along its thickness direction as fitting parameters, to the practically measured electrochemical impedance data. The obtained resistivity profiles implied that the films formed at pHs25℃ 7, 8, 9, 10, and 11 all had a duplex structure; compared to the outer layer in contact with the solution, the inner layer of the film had a much higher resistivity and was inferred to be denser and provide most of the protectiveness of the film. Both the thickness and the total resistance of the film decreased with the increasing solution pH25℃, suggesting that the films formed in more alkaline solutions had less protectiveness against corrosion, consistent with the increasing aluminum alloy corrosion rates previously identified.

Heterogeneous Fusion Design and Perceptive Action in Contemporary Fashion - Focusing on the perspective of Henri Bergson - (현대패션에 나타난 이질적 융합 디자인과 지각(知覺)작용 - Henri Bergson의 시각을 중심으로 -)

  • Kim, Yon-Son;Geum, Key-Sook
    • Journal of the Korean Society of Costume
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    • v.58 no.10
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    • pp.78-94
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    • 2008
  • Contemporary fashion is experiencing a rise in design that combines heterogeneous things, or goes beyond the roles, boundaries, and meanings of existing things. This can be described as a 'heterogeneous fusion' that is different in character from the mixed use of heterogeneous materials, borrowed designs, and exaggeration of the silhouette that have been practices in fashion design, or the non-structure, deconstruction, and recombination that have existed since the age when post-structuralism was a central philosophy. This 'fusion' causes a 'confusion' of the generally accepted mental principle of 'one sense reacting to one stimulus', and breaks the boundary between the various senses, causing confusion in the senses of the individual, and leading him or her to experience unfamiliar feelings. In this process, all information received from external sources is not perceived as it is seen, but rather is perceived through a fusion of the individual's motivations, the environment in which it is perceived, the resulting change in emotion, and the individual's past memories. The combination of these heterogeneous elements visually accepted, or such a non-territorial combination acts as a 'fusion of senses' in the individual's perception, which causes confusion in the homeostasis of perception, and a change in emotion, and serves as a factor that causes the information to be stored in the memory for a long time. In parallel with deconstruction or non-structure, the 'heterogeneous fusion' found in modern fashion is taking root as a representative creative trend, and is represented in various forms such as the mixed use of subjects and materials, non-territorial borrowing, fusion with animal forms, fusion with non-physical geometry, and fusion with heterogeneous hair decoration.

NMR study of the interaction of T$_4$ Endonuclease V with DNA

  • 이봉진;유준석;임형미;임후강
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.267-267
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    • 1994
  • In order to obtain insight into the mechanism by which DNA containing a thymine photo-dimer is recognized by the excision repair enzyme, T$_4$ endonuclease V, we have taken NMR study of this protein and its complex with oligonucleotides. The conformations of five different DNA duplexes DNA I : d(GCGGATGGCG).d(CGCCTACCGC), DNA II d(GCGGTTGGCG) .d(CGCCAACCGC), DNA III : d(GCGGT ^ TGGCG) .d(CGCCAACCGC), DNA IV d(GCGGGCGGCG).d(CGCCCGCCGC) and DNA V d(GCGGCCGGCG) . d(CGCCGGCCGC) were studied by $^1$H NMR. The NMR spectra of these five DNA duplexes in the absence of the enzyme clearly show that the formation of a thymine dimer within the DNA induces only a minor distortion in the structure, and that the overall structure of B type DNA is retained. The photo-dimer formation is found to cause a large change in chemical shifts at the GC7 base pair, which is located at the 3'-side of the thymine dimer, accompanied by the major conformational change at the thymine dimer site. The binding of a mutant T$_4$ endonuclease V (E23Q), which is unable to digest DNA containing a thymine dimer, to the DNA duplex d(GCGGT ^ TGGCG)ㆍd(CGCCAACCGC) causes a large down-field shift in the imino proton resonance of GC7. Therefore, this position is thought to be either the crucial point of the interaction wi th T$_4$ endonuclease V, or the si to of a conformational change in the DNA caused by the binding of T$_4$ endonuclease V. Usually, it is very difficult to assign NMR peaks in DNA * protein complex because of severe peak overlaps. In order to overcome these peak overlaps, we used a method of deuterium incorporation.

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Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]-1 Complex

  • Im, Yu-Jin;Jung, Sang-Mi;Kang, Ye-Song;Kim, Ho-Tae
    • Bulletin of the Korean Chemical Society
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    • v.34 no.4
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    • pp.1232-1236
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    • 2013
  • The Cu cation binding sites of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex have been investigated to explain the $[Cu{\cdot}DNA]$ biological activity caused by the Cu association to DNA. The structure of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex was investigated by electrospray ionization mass spectrometry (ESI-MS). The fragmentation patterns of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex were analyzed by MS/MS spectra. In the MS/MS spectra of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex, three fragment ions were observed with the loss of d(CpG), {d(CpG) + Cyt}, and {d(CpG) + Cyt + dR}. The Cu cation binds to d(CpG) mainly by substituting the $H^+$ of phosphate group. Simultaneously, the Cu cation prefers to bind to a guanine base rather than a cytosine base. Five possible geometries were considered in the attempt to optimize the $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex structure. The ab initio calculations were performed at B3LYP/6-31G(d) level.

Microstructure and Piezoelectric Properties of Low Temperature Sintering PMW-PNN-PZT-BF Ceramics According to PNN Substitution (PNN 치환에 따른 PMW-PNN-PZT-BF 세라믹스의 미세구조와 압전 특성)

  • Sin, Sang-Hoon;Yoo, Ju-Hyun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.2
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    • pp.90-94
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    • 2016
  • In this work, [$Pb(Mg_{1/2}W_{1/2})_{0.03}(Ni_{1/3}Nb_{2/3})_x(Zr_{0.5}Ti_{0.5})_{0.97-x}O_3-BiFeO_3$] (x=0.02 to 0.12) composition ceramics were fabricated by the conventional soild state reaction method and their microstructure and piezoelectric properties were investigated according to PNN substitution. The addition of small amount of $BiFeO_3$, $Li_2CO_3$, and $CaCO_3$ were used in order to decrease the sintering temperature of the ceramics. The XRD (x-ray diffraction patterns) of all ceramics exhibited a perovskite structure. The sinterability of PMW-PNN-PZT-BF ceramics was remarkably improved using liquid phase sintering of $CaCO_3$, $Li_2CO_3$. However, it was identified from of the X-ray diffraction patterns that the secondary phase formed in grain boundaries decreased the piezoelectric properties. According to the substitution of PNN, the crystal structure of ceramics is transformed gradually from a tetragonal to rhombohedral phase. The x=0.10 mol PNN-substituted PMW-PNN-PZT-BF ceramics sintered at $920^{\circ}C$ showed the optimum values of piezoelectric constant($d_{33}$), piezoelectric figure of merit($d_{33{\cdot}}g_{33}$), planar piezoelectric coupling coefficient($k_p$) and density : $d_{33}=566$ [pC/N], $g_{33}=29.28[10^{-3}mV/N]$, $d_{33{\cdot}}g_{33}=16.57[pm^2/N]$, $k_p=0.61$, density=7.82 [$g/cm^3$], suitable for duplex ultrasonic sensor application.

An Analysis on Preference of Interior Coordination Elements for Urban Small Housing - Focused on Gold-Generation(Gold Miss, Gold Mr.) - (도시형 소형주택의 실내코디네이션 요소 선호 분석 - 골드세대(골드미스, 골드미스터) 수요자를 중심으로 -)

  • Kim, So-Hee;Han, Young-Ho
    • Korean Institute of Interior Design Journal
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    • v.21 no.5
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    • pp.264-271
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    • 2012
  • As changed population structure variously, population decrease is one of the important problem in present urbanization. It resulted decline of the medium-large size housings that increasing of 1-2 person household need the variety of small housing with housing type. This study was researched the Gold-Generation on theme by Gold Miss, Gold Mr. who preferring urban small housings that it analyzed survey of Gold generation who be wanted urban small housing by interior coordination elements. Data to be analyzed that first, Gold Mr. and Miss are preferred two bedroom applied by spatial structure coordination. Especially it is based on the separation between livingroom and bedroom with duplex type which is bathroom formed single unit type(shower/toilet/basin). In interior coordination of spatial elements, Gold Mr. considered the form of kitchen with huge ㄷ type instead of Gold Miss are preferred alpha room or alpha space to use powder room or dress room. Second, In Preference of interior coordination elements, Gold Mr. and Miss are preferred bed-clothes and curtains by fabrics, lighting are hanging and spot lighting, accessories are carpet with rug and porcelains. Color are preferred bright scheme both white and ivory, however preference of furniture is different from Gold Mr. and Gold Miss that Gold Mr. are ordered desk, sofa and shelves but Gold Miss are preferred sofa, bed, and dressing table. It showed between Gold Mr. and Gold Miss are equal needs or differences. It expects the basic research for understanding the interior coordination elements for preference in urban small housing as focused on Gold generation(Gold Miss, Gold Mr.) that they will be applied the interior space on urban small housing.

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On the penecontemporaneous deformation structures of the Sinri area at the mid western boundary of the Jinan Basin (진안분지 서변 중앙부 신리지역의 준퇴적동시성 변형구조)

  • Lee Young-Up
    • The Korean Journal of Petroleum Geology
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    • v.6 no.1_2 s.7
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    • pp.8-19
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    • 1998
  • In the Sinri area located at the mid western boundary of the Jinan basin, the Manduksan Formation which mainly consists of coarse sandstone narrowly intercalated with shale and the alternation of sand and shale and the Dalgil Formation mainly of shale are distributed. It consists of four lithofacies, such as coarse sandstone, interbedded sandstone/shale, shale and volcanic rock lithofacies. All sediments are interpreted to be deposited by turbidity currents and free fallouts in a lacustrine basin. In these rocks many penecontemporaneous defomation structures are observed such as fold and thrust fault at large scale, and swelling, boudin structure, flame structure, load structure, ptygmatic fold and convolute bedding at small scale. All these structures are developed between upper and lower undisturbed sedimentary strata. Two large folds are similar folds, but lower one gradually developed into concentric shape. The swelling structures by convergence of the sediments are observed in the hinge area and the boudin structures are developed in the limb. The thrust faults including minor folds and sandstone lobes show duplex structure with asymmetric and kink fold on and below in front of the detached sandstone layer. Development of the swellings, boudins and lobes indicates the flexbility of the sediments during deformational episodes. The folds and thrust faults rarely contain fractures relative their scales and lithologies. This feature also indicates the retrievability of sediments during deformation. At the flanks of the thrust faults the normal faults are formed contemporaneously. The deformation structures at small scale such as flame structures, load structures, ptygmatic folds and convolute beddings are syndepositional and penecontemporaneous, which show the effects of tectonic movements. All these deformed sedimentary structures of the Sinri area suggest the continuing tectonic movements during and/or after deposition.

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Design and Implementation of Dual-Mode SDR Modem Platform (듀얼모드 SDR 모뎀 플랫폼의 설계 및 구현)

  • Yun, Yu-Suk;Choi, Seung-Won
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.4A
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    • pp.387-393
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    • 2008
  • In this paper, we present an SDR (Software Defined Radio) handset modem platform which supports communication systems such as HSDPA (High Speed Downlink Packet Access), and WiBro (Wireless Broadband Portable Internet). The proposed SDR platform employs DSPs (Digital Signal Processors), FPGAs (Field Programmable Gate Arrays), and microprocessors in such a way that the various communication functions like HSDPA and WiBro can be programmed and downloaded to the hardware platform. The proposed SDR platform can be used for functional verification of the physical layers of the mobile handset system in the mobile communication network. We first demonstrate the receiving structure of the physical layer of the HSDPA and WiBro system. Then, the hardware implementation of the proposed SDR platform is shown with functions and optimized signal flows required at each mode. Finally, the link performance of each mode operating on the proposed SDR platform is presented through the internal loopback tests with the test vectors. The experimental performance has been compared with the computer simulation results.