• Title/Summary/Keyword: Dry weight

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Effect of Suspension Culture Conditions on Cell Activity of Wild Viola(Viola partrinii DC.) Callus (야생 흰 제비꽃(viola patrinii DC.)callus의 현탁배양 방법이 세포 활성에 미치는 영향)

  • Kim, Du-Hyun;Chung, Yong-Mo;Chung, Chung-Han;Yeeh, Yeehn;Kwon, Oh-Chang
    • Journal of Life Science
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    • v.6 no.2
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    • pp.94-103
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    • 1996
  • To understand effect of inoculum size, cell density, sucrose concentration and concentrations of MS basal on suspension culture and protoplast isolation of wild viola(Viola patrinii DC.) callus from petiole segments this experiment was conducted. In the lot of 30 mesh inoculum size, two observations were; One was that a considerable increase in the fresh and dry weight of callus was determined. Another was that the callus mass was relatively compact compared with others. A recommendable cell density was 0.4g for 20ml culture medium and the higher sucrose concentration, the higher fresh and dry weight were obtained. The dilution of MS basal salt was differently affected on fresh and dry weight; the highest fresh weight was found in 1x MS salt, while the higest dry weight was in 1/3x dilution.The addition of casein hydrolysate(3g/L) was more effective to increase of both fresh and dry weight. THe contents of protein was great in the inoculum lots with larger inoculum sizeand higher concentration of MS basal salts contenting 3g/L of casein hydrolysate and higher sucrose compared with others. The greatest protoplasts were isolated from the lot of 10 mesh size treated with 1%pectinase SE-150 and 2% cellulase YC. In general, for optimal protoplast isolation the followingconditions were recommended; 1) Use of smaller cell size cultured for 2-5 weeks, and 2) more than 5 hours incubation using the combined mixture of the enzymes with proper concentrations.

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Effect of Seed Weight and Storage Method on Germination and Seedling Growth of Camellia japonica (동백나무의 발아 및 유묘 생장에 미치는 종자 무게 및 저장방법의 영향)

  • Kang, Hee-Kyoung;Choi, Su-Ji;Song, Hong-Seon
    • Korean Journal of Plant Resources
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    • v.33 no.1
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    • pp.33-39
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    • 2020
  • This study was carried out to provide basic information for mass breeding and cultivating the saplings of Camellia japonica. The germination rates did not show definite tendency according to the changes of seed weight at room temperature and 4℃ dry storage conditions. The germination rate of 4℃ wet sand storage was increased with seed weight, but showed very low germination rate for 120 days of storage. The germination rate was above 80% in 60 days, 90 days and 120 days, respectively, at 4℃ wet filter paper storage conditions. The average days taken for those germinations were about 30 days at room temperature and 4℃ dry storage conditions, but wet storage (sand, filter paper) condition took the shortly nearly 13 days for those germinations. Leaf number, leaf dry weight, stem diameter and root dry weight were higher than total average by more than 1.21 g for seed weight. Stem length was higher than the average by more than 1.01 g, plant dry weight was higher than the average by more than 0.81 g, and stem dry weight was higher than the average by more than 0.61 g. In the case of seed weight becoming heavier, seedling growth was good but T/R ratio tended to decrease. In order to increase the mass production of seedlings using Camellia japonica seeds, we need to specify the weight and size of those seeds. The 4℃ wet filter paper storage condition was evaluated as the most efficient method for the seed storage used for seeding.

Sensitivity Analyses of Influencing Factors on Stability in Soil Cut Slope (토사 절토사면 안정성 영향인자의 민감도 분석)

  • Yoo, Nam-Jae;Park, Byung-Soo;Jun, Sang-Hyun;Cho, Han-Ki
    • Journal of Industrial Technology
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    • v.26 no.B
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    • pp.73-81
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    • 2006
  • A sensitivity analysis about effects of influencing factors on the stability of Soil cut slope was performed. Slope stability analyses were carried out under dry, rainy and seismic conditions. Dominant factors controlling the slope stability were chosen such as cohesion and internal friction angle, unit weight of soil, water table and seismic horizontal coefficient used for the slope stability during earthquake. Parametric stability analysis with those factors was performed for sensitivity analysis. As results of analyzing the sensitivity of factors under dry and rainy conditions, effects of cohesion, internal friction angle and unit weight of soil on the stability of slope are more critical in the dry condition than in the rainy condition. Cohesion and internal friction angle are more dominant factors influencing the slope stability irrespective of dry or rainy conditions than unit weight of soil and the horizontal seismic coefficient. The unit weight and the horizontal seismic coefficient affects crucially the stability according to conditions of slope formation and dry or rainy seasons. For the effect of horizontal seismic coefficient on stability of slope, safety factor of slope is not affected significantly by dry or rainy conditions. However, increase of the horizontal seismic coefficient under the rainy condition floes reduce the safety factor significantly rather than the dry condition. Therefore, it is needed that the location of the water table is assigned appropriately to satisfy the required safety factor of stability in the case of checking slope stability for the rainy and seismic conditions.

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Improvement of Liquid Culture Methods of Paecilomyces japonica (눈꽃동충하초균의 액체배양법 개선)

  • 남성희;정이연;조세연;한명세
    • Journal of Sericultural and Entomological Science
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    • v.43 no.1
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    • pp.33-36
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    • 2001
  • This study was carried out to improve the liquid culture methods of Paecilomyces japonica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150rpm under the photoperiod of 12L-12D. Dry weight of mycelium was averagely 1.216 g in the Sikworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6 g of 6 mmbeads in the SL medium, the dry weight is increased to 1.332 g. The optimal addition of silkworm larval powder to the culture medium for gest harvest was 1.360$\pm$0.67 g in dry weight.

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Characteristics of Waesungri Maize (Zea mays L.) Inbred with Multi Tillers and Ears for Crude Forage Use

  • Lee, Hee-Bong
    • Plant Resources
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    • v.4 no.1
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    • pp.31-35
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    • 2001
  • Major characteristics of new Waesungri maize inbred line has multi-tiller and ears: five to six tillers and seven to eight ears per plant and flowering date of Waesungri was delayed about 18 and 24 days compared to Mo17 U.S line and IK$_4$ Korean local lines, respectively. Number of ears, fresh and dry weight per plant were significantly different among all tested hybrids including Waesungri/Sinkihong hybrid under different planting times and densities. Especially, both fresh and dry weight of IK$_1$/FR140//Waesungri F$_1$hybrid were significantly higher at high planting density. In kernel weight per unit area, Wnesungri/Sinkihong hybrid was high at high density and IK$_1$/FR140//Waesungri hybrid was high at low planting density. As results of analysis of variance, flowering date was shown a significantly different both planting times and varieties, while other characters including stem height were shown very variable in interactions with enviromental factors.

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Studies on Dry Matter Production and Variation of Agronomic Characteristics of Determinate and Indeterminate Types of Soybean Cultivars (Glycine max L.) Under Different Growing Condition (유ㆍ무한형대두품종의 재배조건에 따른 건물생산 및 형질변이에 관한 연구)

  • Keun-Yong Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.45-78
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    • 1974
  • To provide useful information for developing new high yielding soybean varieties and for improving cultural practices, an investigation was made on variation of dry matter production and on relationship among several agronomic characters of soybean plants grown under different planting times and densities as well as under different fertilizer levels, using Kwang-kyo, Dong puk-tae, and Suke # 51 as determinate types and Shelby, SRF-300 and Harosoy as indeterminate types at the Crop Experiment Station during the period of 1972 and 1973. The results obtained were summarized as follow: 1. The dry weight, CGR and LAI at the initial flowering stage were high in the high plant population irrespective of varieties, planting times, and fertilizer levels. However, those characters of the indeterminate type were lower than those of the determinate types. The same characters of the indererminate type at the terminal leaf stage were either same or higher than those of the determinate types. 2. The dry weight of the determinate type at the initial flowering stage was similar to the indeterminate, type, when planting times were May 21 or June 15. The dry weights of both types of varieties were low when planted on July 10. When fertilizer levels were increased, the CGR, dry weight and LAI at the initial flowering stages were also increased. 3. Even though significant differences of LAI were obtained among the varieties within the same plant type, the indeterminate type was in general lower than that of the determinate type regardless of planting time and densities, or fertilizer levels, while the yield of the indeterminate type was comparable to the yield of the determinate type. 4. The high degree of leaf- and petiole-fall at the greenbean stage was highly associated with early planting and high levels of fertilizers. However, less amount of leaf- or petiole-fall was found when planted on July 10 or under low plant population. 5. The percent of stem weight was high under higher plant population, while the percent of leaf weight was high under lower plant population. When planting time was late, the percent of stem and petiole weight were reduced, while the leaf weight was increased. 6. The percent of pod weight of the determinate type at the terminal leaf stage was about 2% when planted on May 21, about 8% when planted on June 15, and about 9% when planted on July 10. The percent of pod weight of the indeterminate type at the terminal leaf stage were about 6 % when planted on May 21, 14% when planted on June 15 and 21% when planted on July 10. 7. Kwang kyo showed less degree of leaf-fall even when lodged due to high levels of fertilizer applied, while SRF-300 showed great damage due to lodging. 8. High yields were obtained when planted on May 21, but there were little yield differences between yields from May 21 and June 15 plantings. The reduction of yield due to late planting of July 10 was less apparent in the determinate type of varieties, while it was high in the indeterminate type. 9. The optimum plant population per are for high yield was 1, 250 to 2, 500 plants when planted on May 21, 2, 500 plants when planted on June 15, and 3, 333 plants when planted on July 10. 10. High correlation coefficients were obtained between dry matter weight and LAI at the terminal leaf stages, and between the dry matter weight and yield at the greenbean stages. The optimum dry weight for high yield in the determinate type was expected to be 25 kg. per are at the initial flowering stage and 50 kg. per are at the terminal leaf stage. In the indeterminate type the LAI and dry weight at the greenbean stage were 4 to 5 and 80 kg. per are, respectively. 11. Under the high plant population plant height was increased, while the stem diameter and the number of nodes and branches were reduced. Consequently, the percent of mainstem to main stem plus branches were increased, and the length of internode was also elongated. The ratios of stem weight, number of nodes and pods, and yield of main stem were increased when high plant population was associated with the early planting. The percent of main stem to branches for the indeterminate type was higher than that of the determinate type. 12. Under the high plant densities and late planting, the percent of the pod number and yields of main stem were increased, indicating that varieties with no or less branches were better adaptable under such conditions. 13. High degree of simple correlation coefficients was obtained between the LAI at the initial flowering stage and terminal leaf stage, and the total node number, dry matter and dry stem weight of both determinate and indeterminate types. Even though no significant correlation was found between the LAI at the initial flowering stage of the determinate type and the stem length and pod number per are, highly significant correlation coefficients were obtained between such characters in the indeterminate type of varieties. 14. The dry matter was positively correlated with the LAI, CGR, stem length, and pod number, node number and dry stem weight per are, while no significant correlation was found between the dry matter and stem diameter. 15. The correlation coefficients between lodging index and the LAI, dry weight, stem length and dry stem weight were highly significant. Negative correlation was obtained for the indeterminate type between the stem diameter and lodging index. The correlation coefficient between the stem diameter and lodging index was non-significant for the determinate type, while positive correlation was obtained between the yield and lodging index in the determinate type. The lodging index was also positively correlated with average length of internode of main stem. 16. The 100 seed weight appeared to be lowered under the high plant population and no fertilizer condition, and when planted late. Apparent differences of 100 seed weight were found between main stem and branches, being higher for the main stem than for the branches. 17. No variation of protein content was found due to different cultural practices. However, the oil content was apparently reduced when planted late.

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Varietal Difference in Feed Value of Rice Straw and Its Relationship with Agronomic Traits (볏짚 사료가치의 품종간 차이 및 생육형질과의 관련성)

  • Kim Chang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.516-521
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    • 2004
  • The straw of thirty one rice varieties were evaluated for their feed value and related agronomic traits. The rice straw were hand-harvested, dried to constant weight at $75^{\circ}C$ and ground through a 20 mesh seive in a Wiley mill, analyzed with crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF). Relative feed value (RFV) was calculated from NDF and ADF. The sum of standardized score was estimated by dry weight of rice straw, content of CP, ADF and NDF. The straw yield of Daeanbyeo was 725.9 kg/10a, showed heighest value among the varieties and remainder was in the order of Keumnambyeo, Donginbyeo #1 and Chucheongbyeo. Crude protein (CP) content in a Dasanbyeo was higher than those in other varieties. The content of ADF in a Junghwabyeo and NDF in a Sobaegbyeo were $34.3\%$ and $63.8\%$, respectively, showed lowest value among the varieties. The rice straw of Dunnaebyeo, Obongbyeo, Seoanbyeo, Keumobyeo, Hwaseongbyeo, Noganbyeo and Gyehwabyeo belonged to the high feed value varieties by estimation of cluster analysis, sum of standardized score and RFV. The content of CP was found to be positively related with dry weight of leaf and grain, but negatively related with heading days after seeding, culm length, specific leaf weight (SLW) and dry weight of stem. ADF and NDF were found to be positively related with heading days after seeding, culm length, SLW and dry weight of leaf, but negatively related with dry weight of stem. The sum of standardized score and RFV were the only positive relationship with dry weight of stem and negative relationship with other traits.

Prediction of Dry Matter Intake of Hanwoo Bulls (한우 수소의 건물섭취량 추정)

  • Lee, S.C.;Moon, Y.H.;Lee, H.J.;Oh, Y.G.;Kim, K.H.
    • Journal of Animal Science and Technology
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    • v.44 no.3
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    • pp.341-350
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    • 2002
  • Twenty four Hanwoo bulls(mean initial body weight 162$\pm$8.1kg) were employed to induce the regression equations on body weight gain and dry matter intake. The diets were fed in a 3${\times}$4 factorial arrangement; three roughage sources(rice straw, hay, corn silage) and four roughage ratios in ration(100, 60, 40, 20%). Bulls were alloted by incomplete turn over design with four replications. Daily body weight gain was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. Bulls fed hay or corn silage only as feed kept in their body weight gain during the whole experimental period, however those fed rice straw only were in a negative body gain hereafter fatteningⅠperiod(350kg of body wt.). Dry matter intake per metabolizable body weight was decreased with increasing body weight. Dry matter intake was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. The regression equations on daily body weight gain(DG, kg) and dry matter intake(DMI, kg/day) according to body weight(BW, kg) of Hanwoo bulls were derived as follows; DG = -0.842 + 17.5DMI/$BWkg^{0.75}$ DMI = 4.125 + 0.013BW + 1.075DG - 0.045NDF where, NDF is the percentage of neutral detergent fiber in ration.

Relationship between Specific Stubie Weight and Regrowth of Perennial Ryegrass (Perennial ryegrass의 그루터기 면적중과 재생과의 관계)

  • 이주삼;김성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.2
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    • pp.90-96
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    • 1991
  • This experiment was carried out in order to estimate the recovery days of root and stubble to the days after cutting, and contribution of specific stubble weight on the regrowth was examined using the relationships between the dry weight of shoot and yield components, and regrowth parameters by the days after cutting. The varieties examined were Maprima, Manhattan, Tove, Peramo, Caliente, Tempo and P-2 grown under individual plant basis. The results are may be summarized as follows: 1. Dry weight of root and stubble were recovered up to 13.5 and 11 days after cutting, respectively. 2. Dry weight of shoot(regrowth parts+stubble) was affected significantly by the varieties, stages of regrowth and variety x stage of regrowth. 3. The variety with tiller weight type showed higher average productivity of shoot than those of the variety with tiller number type. 4. Absolute growth rate(AGR) of shoot was correlated significantly with regrowth parts, stubble, root and weight of a tiller at the early stage of regrowth(up to 12 days after cutting), and correlated with regrowth parts, stubble, weight of tiller and stubble area at the late stage of regrowth(up to 20 days after cutting). 5. Contribution of specific stubble weight to absolute growth rate of shoot was different between the stages of regrowth. Thus, regrowth parts per specific stubble weight(RP1SSbW) and weight of tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the early stage of regrowth, and efficiency of specific stubble weight(ESSbW), regrowth parts per specific stubble weight (RPISSbW) and weight of a tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the late stage of regrowth. 6. Regrowth utilization rate(RUR) was one of the useful regrowth parameter to indicate the regrowth potential of grasses.

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Horizontal Distribution of Dinoflagellate Resting Cysts in Sediments from the Southeastern Yellow Sea (황해 남동부 해역 저질 내 와편모조류 휴면포자의 분포)

  • Hwang, Choul-Hee;Heo, Seung;Kim, Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.1
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    • pp.68-72
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    • 2009
  • To gain a greater understanding of the potential for future harmful algal bloom (HAB) outbreaks and to trace the dispersal paths of dinoflagellates, sediment samples were collected from 13 stations in the southeastern Yellow Sea. 23 different types of dinoflagellate resting cysts were identified from the samples. Protoceratium reticulatum (1-391 cells/g dry weight), Gonyaulax scrippsae (0-254 cells/g dry weight), G. spinifera (0-301 cells/g dry weight) and Alexandrium spp. (ellipsoidal type) (0-76 cells/g dry weight) were the dominant species at all surveyed stations. The overall distribution pattern demonstrated that the resting cyst densities were highest in the offshore area and decreased gradually toward the Korean coast. On the other hand, the composition rate of resting cysts of the heterotrophic dinoflagellate species to the total dinoflagellates was higher in the Korean coast region than in the offshore area. We supposed that this distribution pattern of dinoflagellate resting cysts appeared to be influenced by the hydrographic features and environmental conditions of the Yellow Sea.