• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.989-992
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• 2003

Recombinant human tissue plasminogen activator deletion mutein (Reteplase) is a clinically promising thrombolytic drug. Reteplase cDNA was subcloned into a bacteria expression system, and the resultant recombinant was biologically characterized. The Reteplase was expressed in Escherichia coli as an inclusion body, and the downstream processes of the Reteplase inclusion body included denaturation, renaturation, and purification. A protein disulfide isomerase (PDI) was used to assist the refolding of Reteplase, and it was found to increase the refolding rate from less than 2％ to more than 20％. The refolded Reteplase was purified through two chromatography steps, including lysine-coupled agarose affinity chromatography and then CM-sepharose cation-exchange chomatography. The purity of r-PA was analyzed by Western bolt analysis, and N-terminal amino acid and amino acid composition analyses confirmed the end-product. Reteplase showed higher thrombolytic potency in an animal thrombus model.

• International Journal of Automotive Technology
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• 제5권3호
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• pp.145-153
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• 2004

Potential fuel economy improvements and environmental legislation have renewed interest in Gasoline Direct Injection (GDI) engines. Computational models of fuel injection and mixing processes pre-ignition are being developed for engine optimisation. These highly transient thermofluid models require verification against temporally and spatially resolved data-sets. The authors have previously established the capability of PDA to provide suitable temporally and spatially resolved spray characteristics such as mean droplet size, velocity components and qualitative mass distribution. This paper utilises this data-set to assess the predictive capability of a numerical model for GDI spray prediction. After a brief description of the two-phase model and discretisation sensitivity, the influence of initial spray conditions is discussed. A minimum of 5 initial global spray characteristics are required to model the downstream spray characteristics adequately under isothermal, atmospheric conditions. Verification of predicted transient spray characteristics such as the hollow-cone, cone collapse, head vortex, stratification and penetration are discussed, and further improvements to modelling GDI sprays proposed.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.471-481
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• 2005

Heavy crudes (bitumen) are extremely viscous and contain high concentrations of asphaltene, resins, nitrogen and sulfur containing heteroaromatics and several metals, particularly nickel and vanadium. These properties of heavy crude oil present serious operational problems in heavy oil production and downstream processing. There are vast deposits of heavy crude oils in many parts of the world. In fact, these reserves are estimated at more than seven times the known remaining reserves of conventional crude oils. It has been proven that reserves of conventional crude oil are being depleted, thus there is a growing interest in the utilization of these vast resources of unconventional oils to produce refined fuels and petrochemicals by upgrading. Presently, the methods used for reducing viscosity and upgradation is cost intensive, less selective and environmentally reactive. Biological processing of heavy crudes may provide an ecofriendly alternative or complementary process with less severe process conditions and higher selectivity to specific reactions to upgrade heavy crude oil. This review describes the prospects and strengths of biological processes for upgrading of heavy crude oil.

• 한국분무공학회지
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• 제11권4호
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• pp.244-250
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• 2006

Of the macroscopic spray characteristics of non-reacting diesel fuel sprays, the spray angle reflects directly the atomization and air entrainment processes downstream the nozzle. In addition, spray angle is important because it will be closely related to the spray penetration. The existing definitions for the measurement of spray angle as well as the correlations for the prediction of spray angle are, therefore, summarized and reviewed. The existing definition of spray angle can be classified into four groups: distance based on orifice diameter, distance based on spray tip penetration, definition based on surface wave, and definition based on atomization. It is strongly required to specify the definition and measurement method when the data for spray angle is reported. The existing correlations for spray angle can be classified into two groups: theoretical and empirical correlations. The study on the evaluation of the existing correlations fer spray angle is required.

• 한국지구과학회지
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• 제27권7호
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• pp.757-767
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• 2006

Alluvial-plain deposits in the southeastern part of the Eumsung Basin (Cretaceous) are characterized by coarse-grained channel fills encased in purple siltstone beds. It represents distinct channel geometry, infill organization, and variations in facies distribution. The directions of paleocurrent, sedimentary facies changes, and channel-fill geometry can be used to reconstruct a channel network in the alluvial system developed along the southeastern margin of the basin. The channel-fill facies represent downstream changes: 1) down-sizing and well-sorting in clast and martix of channel fills and 2) internal organization of scour fill or gravel lag and overlying cross-stratified, planar-stratified beds. These findings suggest multiple stages of channel-filling processes according to flooding and subsequent stream flows. In the small-scale pull-apart Eumsung Basin (${\sim}7{\times}33km^2$ in area), vertical-stacked alluvial architecture of the coarse-grained channel fills encased in purple siltstone is expected to result from episodic channel shifting under a rapidly subsiding setting.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2012년도 제44회 KOSCO SYMPOSIUM 초록집
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• pp.253-255
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• 2012

This study has numerically investigated the combustion processes in the bilayer porous media. To account for the velocity transition and diffusion influenced by solid matrix, porosity effects are included in the governing equations. Heat transfer coefficient is calculated by Nusselt number to reflect the effect of gas velocity, pore diameter, and material properties. Numerical results indicate that the present approach is capable of the essential features of the premixed combustion in the porous burner, in terms of the precised flame structure, pollutant formation, and flame stabilization. It is also found that heat transferred from the downstream flame zone is conducted to the upstream flame region through the solid matrix and the preheated mixture. By increasing the inlet velocity, the solid temperature of upstream is cooling down.

• Molecules and Cells
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• 제28권1호
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• pp.1-5
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• 2009

Vascular endothelial growth factor (VEGF) is essential for many angiogenic processes both in normal and pathological conditions. However, the signaling pathways involved in VEGF-induced angiogenesis are incompletely understood. The protein kinase D1 (PKD1), a newly described calcium/calmodulin-dependent serine/threonine kinase, has been implicated in cell migration, proliferation and membrane trafficking. Increasing evidence suggests critical roles for PKD1-mediated signaling pathways in endothelial cells, particularly in the regulation of VEGF-induced angiogenesis. Recent studies show that class IIa histone deacetylases (HDACs) are PKD1 substrates and VEGF signal-responsive repressors of myocyte enhancer factor-2 (MEF2) transcriptional activation in endothelial cells. This review provides a guide to PKD1 signaling pathways and the direct downstream targets of PKD1 in VEGF signaling, and suggests important functions of PKD1 in angiogenesis.

• BMB Reports
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• 제51권3호
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• pp.106-118
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• 2018

How the organ size is adjusted to the proper size during development and how organs know that they reach the original size during regeneration remain long-standing questions. Based on studies using multiple model organisms and approaches for over 20 years, a consensus has been established that the Hippo pathway plays crucial roles in controlling organ size and maintaining tissue homeostasis. Given the significance of these processes, the dysregulation of the Hippo pathway has also implicated various diseases, such as tissue degeneration and cancer. By regulating the downstream transcriptional coactivators YAP and TAZ, the Hippo pathway coordinates cell proliferation and apoptosis in response to a variety of signals including cell contact inhibition, polarity, mechanical sensation and soluble factors. Since the core components and their functions of the Hippo pathway are evolutionarily conserved, this pathway serves as a global regulator of organ size control. Therefore, further investigation of the regulatory mechanisms will provide physiological insights to better understand tissue homeostasis. In this review, the historical developments and current understandings of the regulatory mechanism of Hippo signaling pathway are discussed.

• 천문학회보
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• 제36권1호
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• pp.45.1-45.1
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• 2011

Shock waves form in the intergalactic medium as a consequence of accretion, merger, and turbulent motion during the structure formation of the universe. They not only heat gas but also govern non-thermal processes through the acceleration of cosmic rays (CRs), production of magnetic fields, and generation of vorticity. We examine diffusive shock acceleration of the pre-existing as well as freshly injected populations of nonthermal, CR particles at weak cosmological shocks. Since the injection is extremely inefficient at weak shocks, the pre-existing CR population dominates over the injected population. If the pressure due to pre-existing CR protons is about 5 % of the gas thermal pressure in the upstream flow, the downstream CR pressure can absorb typically a few to 10 % of the shock ram pressure at shocks with the Mach number M<3. Yet, the re-acceleration of CR electrons can result in a substantial synchrotron emission behind the shock. The implication of our findings for observed bright radio relics is discussed.

• 소성∙가공
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• 제13권7호
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• pp.614-622
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• 2004

A new approach for the automatic elimination of free edges in the finite element model for the analysis of sheet metal forming processes is presented. In general, the raw finite element model constructed from an automatic mesh generator is not well suited for the direct use in the downstream forming analysis due to the many free edges which requires tedious time consuming interactive graphic operations of the users. In the present study, a general method for the automatic elimination of free edges is proposed by introducing a CAD/CAE hybrid method. In the method a trimmed parametric surface is generated to fill the holes which are orginated from the free edges by using the one step elastic finite element analysis. In addition, mesh generation algorithm is suggested which can be used in the general trimmed surface. In order to verify the validity of the proposed method, various examples including actual automobile sheet metal parts are given and discussed.