• Journal of the Korean Vacuum Society
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• v.7 no.4
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• pp.348-354
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• 1998

To investigate an influence of the thermal preheating for the substrates exerted on the heteroepilayers, the ZnTe epilayers are grown on the GaAs (100) at the substrate temperature of 450~$630^{\circ}C$ by hot wall epitaxy (HWE). For this purpose, double crystal rocking curve (DCRC) and photoluminescence (PL) are measured. The full width at half maximum of DCRC are the smallest in the ZnTe epilayers grown on the GaAs thermally etched at around both $510^{\circ}C$ and $590^{\circ}C$. However, at around $550^{\circ}C$ they increase due to the reconstruction of the atoms in the surface. And they increase due to the oxide layer at below $490^{\circ}C$ and due to the surface defects at above $610^{\circ}C$. From PL analysis, the full width at half maximum of the light hole exciton $X_{1s,th}$ and of the second-order Raman line increase at around $550^{\circ}C$. With the increasing preheating temperature, the intensities of Y-bands and of the oxygen bound exciton (OBE) peak related to an oxide layer on the GaAs surface generally decrease. From these experimental results, it's confirmed that the GaAs substrate thermally etched influences the ZnTe pilayers.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3301-3306
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• 2015

Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of human non-small cell lung cancer (NSCLC) cells. We investigated the role of C23 in activating the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), which is a critical protein for DNA double-strand breaks (DSBs) repair. As a result, we found that the expression of C23 was negatively correlated with the radiosensitivity of NSCLC cell lines. In vitro clonogenic survival assays revealed that C23 knockdown increased the radiosensitivity of a human lung adenocarcinoma cell line, potentially through the promotion of radiation-induced apoptosis and adjusting the cell cycle to a more radiosensitive stage. Immunofluorescence data revealed an increasing quantity of ${gamma}$-H2AX foci and decreasing radiation-induced DNA damage repair following knockdown of C23. To further clarify the mechanism of C23 in DNA DSBs repair, we detected the expression of DNA-PKcs and C23 proteins in NSCLC cell lines. C23 might participate in DNA DSBs repair for the reason that the expression of DNA-PKcs decreased at 30, 60, 120 and 360 minutes after irradiation in C23 knockdown cells. Especially, the activity of DNA-PKcs phosphorylation sites at the S2056 and T2609 was significantly suppressed. Therefore we concluded that C23 knockdown can inhibit DNA-PKcs phosphorylation activity at the S2056 and T2609 sites, thus reducing the radiation damage repair and increasing the radiosensitivity of NSCLC cells. Taken together, the inhibition of C23 expression was shown to increase the radiosensitivity of NSCLC cells, as implied by the relevance to the notably decreased DNA-PKcs phosphorylation activity at the S2056 and T2609 clusters. Further research on targeted C23 treatment may promote effectiveness of radiotherapy and provide new targets for NSCLC patients.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.778-784
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• 2017

In this paper, we propose wired and wireless distributed control systems designed to improve the freight logistics efficiency and verify wired distributed control systems. The verification condition required that 50 cargo vehicles be connected and operated to travel 21 km from Busan Sinhang station to Jinlye Station at an average speed of about 100km/h. The verification results show that the traction output and braking output of the control and controlled cars are dispersed by the wired distributed control system. The application is expected to more than double the efficiency of the logistics compared to the existing freight transportation system. However, in the case of the wired distributed control system, cable installation and maintenance are difficult, and it is impossible to change the combination of freight vehicles. Through the verification of the wired distributed control system, the applicability of distributed control systems to freight vehicles in Korea was confirmed and the system was further developed to produce a wireless distributed control system. In order to apply the wireless distributed control system, a propagation environment analysis for the ISM band was performed in the testbed and, as a result, it was confirmed that Wifi technology using the ISM band could be utilized. In order to use the WDP (Wireless Distributed Power) devices newly installed in the target vehicles, the transmission / reception control signals associated with the propulsion / braking / total control devices are defined. In the case of wireless distributed control systems, the convenience of their application and operation is guaranteed, but reliability and emergency safety measures should because of the dependence of the control of the vehicle on radio signals.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.152-172
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• 1992

The ship sailing among waves are suffered the various wave loads that comes from its motion throughout its life. Because there are dynamic, the analysis of ship structure must be considered as the dynamic problem precisely. In the rationally-based design, the dynamic structural analysis is carried out using dynamic wave loads provided from the results of the ship mouton calculation as the rigid body. This method is based on the linear theory assumed low wave height and small amplitude of motion. But at the rough sea condition, high wave height, relatively ship's depth, is induced the large ship motion, so the ship section configulation below water line is rapidly changed at each time. This results in non-linear problem. Considering above situation in this paper, the strength analysis method is introduced for the hull glider among waves considering non-linear hydrodynamic forces. This paper considers that the overall or primary level of the ship structural dynamic loading and dynamic response provided from the non-linear wave forces, and bottom and bow flare impact forces estimated by momentum slamming theory, in which the ship is idealized as a hollow thin-walled box beam using thin-walled beam theory and the finite element method. This method is applied to 40,000 Ton Double-Skin Tanker and attention is paid to the influence of the response of ship speed, wave length and wave height compared with linear strip theory.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.44-48
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• 2015

This study was conducted to evaluate the efficacy of weed control and phytotoxicity of oxyfluorfen using a transgenic rice line (CPPO06 event) resistant to protoporphyrinogen oxidase (Protox) inhibiting herbicides in the direct-seeding flooded rice. Five annual weeds including Echinochloa oryzoides and two perennial weeds were occurred in the test field. Oxyfluorfen at 120 g a.i. $ha^{-1}$ in the application timing of two days before sowing and 0 and five days after sowing showed more than 90% weed control value except for Juglans mandshurica. Total weed control value was more than 95% in any application timing indicating a highly effective herbicide in the direct-seeding flooded rice. When compared with untreated control, no visual injuries were detected at single and double dosage of oxyfluorfen. The agronomic characteristics and yield components of CPPO06 event was reasonable in any time of application in this study. Based on these data, oxyfluorfen application before and after sowing can be applied to provide effective weed management in the direct-seeding flooded field.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.443-450
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• 2009

Purpose: Conventional image reconstruction uses simplified physical models of projection. However, real physics, for example 3D reconstruction, takes too long time to process all the data in clinic and is unable in a common reconstruction machine because of the large memory for complex physical models. We suggest the realistic distributed memory model of fast-reconstruction using parallel processing on personal computers to enable large-scale technologies. Materials and Methods: The preliminary tests for the possibility on virtual manchines and various performance test on commercial super computer, Tachyon were performed. Expectation maximization algorithm with common 2D projection and realistic 3D line of response were tested. Since the process time was getting slower (max 6 times) after a certain iteration, optimization for compiler was performed to maximize the efficiency of parallelization. Results: Parallel processing of a program on multiple computers was available on Linux with MPICH and NFS. We verified that differences between parallel processed image and single processed image at the same iterations were under the significant digits of floating point number, about 6 bit. Double processors showed good efficiency (1.96 times) of parallel computing. Delay phenomenon was solved by vectorization method using SSE. Conclusion: Through the study, realistic parallel computing system in clinic was established to be able to reconstruct by plenty of memory using the realistic physical models which was impossible to simplify.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.5
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• pp.13-22
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• 2018

In this study, a governing differential equation for the bending problem of orthotropic rectangular plate is drived. It's exact solution for various boundary conditions is presented. This solution follows traditional method like Navier's solution or Levy's solution that transforms the governing differential equation into an algebraic equation by using trigonometric series. To obtain a solution by Levy's method, it is required that two opposite edges of the plate be simply supported. And the boundary conditions, for which the Navier's method is applicable, are simply supported edge at all edges. In this study, it overcomes the limitations of the previous Navier's and Levy's methods.This solution is applicable for any combination of boundary conditions with simply supported edge and clamped edge in x, y direction. The plate could be subjected to uniform, partially uniform, and line loads. The advantage of the solution is that it is the exact solution as well as it overcomes the limitations of the previous Navier's and Levy's methods. Calculations are presented for orthotropic plates with nonsymmetric boundary conditions. Comparisons between the result of this paper and the result of Navier, Levy and Szilard solutions are made for the isotropic plates. The deflections were in excellent agreement.

• Journal of Life Science
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• v.21 no.11
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• pp.1573-1578
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• 2011

Milk thistle (silybum marianum) is a famous dietary supplement widely used in the United States and Europe. Silbinin is a major biologically active compound of milk thistle and has strong antioxidant and radical scavenger activities. Anticancer activities, as well as chemopreventive effects on various cancer cell lines, including prostate, lung, colon, skin, and bladder, have also been reported in silbinin. In the present study, we investigated the anticancer effects of silibinin and apoptosis through cell cycle arrest on prostate cancer cell PC-3. We performed cell viability by MTT assay and western blotting to confirm cell cycle check point proteins such as cyclin A/D1/E and cyclin-dependent kinase (CDK) 2/4/6. To quantify silibinin-induced apoptotic cell death of PC-3, Annexin V and PI double staining was performed by flow cytometry, by which its cell distribution was determined. As a result, silibinin inhibited the cell growth of PC-3 cells in a time- and dose-dependent manner, and its treatment resulted in cell cycle arrest at the G1 phase. Also the level of cell cycle check point proteins (cyclin, CDK) was decreased by silibinin in a dose-dependent manner. Taken together, we suggest that apoptosis of prostate cancer cell line PC-3 induced by silibinin is associated with cell cycle arrest through decrease of cell cycle check point proteins, caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage.

• Food Science and Preservation
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• v.20 no.5
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• pp.691-698
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• 2013

The biological activities of Smilax china L. rhizome (SCR), hot water (SCRW) and 70% ethanol extract (SCRE) were analyzed. The total phenolic contents of SCRW and SCRE were 51.7 and 100.5 mg/g, respectively. The measured flavonoid content of SCRW ($67.7{\mu}g/g$) was almost double that of SCRE ($31.7{\mu}g/g$). SCRE ($IC_{50}=42.4{\mu}g/mL$) exhibited stronger antioxidant activity in the DPPH system than the positive control ${\alpha}$-tocopherol ($71.3{\mu}g/mL$) or butylated hydroxy anisole ($53.8{\mu}g/mL$) did. SCRE ($IC_{50}=50.3{\mu}g/mL$) also showed stronger ABTS radical scavenging activity, as did ${\alpha}$-tocopherol ($67.1{\mu}g/mL$). The SOD-like activity and Tyrosinase inhibition activity of SCRW and SCRE showed almost the same pattern. The best SOD-like activity and tyrosinase inhibition activity were measured as 24.9% and 20.3% in SCRW at $1,000{\mu}g/mL$, respectively. The cytotoxic effects of the SCR extracts were analyzed via MTT assay on human cancer and normal cells. SCRW and SCRE did not show cytotoxicity up to the concentration of $1,000{\mu}g/mL$ against the normal human cell line HEK293. Against human breast cancer cells (MCF-7), SCRW inhibited MCF-7 growth (by 27.6%) better than the anticancer drug cyclophosphamide (15.5%) at $1,000{\mu}g/mL$. SCRE ($1,000{\mu}g/mL$) inhibited the growth of human lung cancer cells A549 (37.6%) and human stomach cancer cells AGS (53.6%) more effective than did SCRW (21.0% and 35.4%) or CPA (22.2% and 31.7%). These results suggest the potential use of SCRE and SCRW as an excellent antioxidant and antiproliferative substance, respectively.

• Korean Journal of Acupuncture
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• v.24 no.1
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• pp.171-187
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• 2007

Objectives : This study was performed to determine if Orostachys japonicus A. Berger herbal acupuncture (OjB) provides the protective effect against the loss of cell viability and DNA damage induced by oxidant in renal proximal tubular cells. Methods : The cell viability was evaluated by a MTT reduction assay and DNA damage was estimated by measuring double stranded DNA breaks in opossum kidney (OK) cells, an established proximal tubular cell line. Lipid peroxidation was determined by measuring malondialdehyde (MDA), a product of lipid peroxidation. Results : H2O2 increased the loss of cell viability in a time-dependent manner, which were prevented by 0.1% OjB. The protective effect of OjB was dose-dependent over concentration range of 0.05-0.5%. H2O2 caused ATP depletion and DNA damage, which were prevented by OjB and the hydrogen peroxide scavenger catalase. The loss of cell viability by H2O2 was not affected by the antioxidant DPPD, but lipid peroxidation by the oxidant was completely inhibited by DPPD. Generation of superoxide and H2O2 in neutrophils activated by phorbol-12,13-dibutyrate was inhibited by OjB in a dose-dependent manner. OjB inhibited generation of H2O2 in OK cells treated with antimycin A and exerted a direct H2O2 scavenging effect. Exposure of OK cells to 1 mM tBHP caused a significant depletion of glutathione which was prevented by OjB. OjB accelerated the recovery in cells cultured for 20 hr in normal medium without oxidant following oxidative stress. Conclusions : These results suggest that OjB exerts the protective effect against oxidant-induced cell injury and its protective effect was resulted from radical scavenging and antioxidant activities.