• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.2
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• pp.185-196
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• 1988

In this study, a method of nonlinear dynamic analysis of suspension bridges subjected to random wind loads is pre.sented. The nonlinearity considered is the one due to the interaction between the motion of the bridge girder and the tertsion variation of the main cables. The equation of motion is formulated using a continuum approach. The coupling between the vertical and torsional motions are included in the analysis. The equation of motion is solved by using the mode superposition method. The analysis is carried out in the frequency domain utilizing the stochastic linearization technique on to the modal equations. In the linearization procedure, the nonlinear terms are approximated as linear ones with constant terms. The verification of the method has been performed on a case with four modal degrees of freedom. Example analyses are carried out on two suspension bridges for various wind speeds and wind force parameters. Numerical results indicate that, by including the nonlinearity into the analysis, the dynamic responses of the bridges, particularly in the vertical direction, change considerably.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.10 no.2
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• pp.64-72
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• 1998

Dynamic response analysis is carried out for slender marine structures such as tensioned risers and tethers of tension leg platform, which are subjected to floating vessel motions as well as environmental forces arising from ocean waves. A mumerical analysis procedure is developed by using finite element model of the structural member. Dynamic analses are performed in the time domain for regular waves. Parameter studies are carried out to highlight the effects of surface vessel motions on the lateral dynamics of the structures. Example results of displacements, bending stresses are compared for various in water depth, environmental condition and vessel motion. Some instability conditions of the structures due to time-varying tension by vessel heave motion are discussed through the example analyses. As the results, the interaction between vessel surge and heave motions amplifies the total structural response of a riser. In the case of a tether, the effect of vessel heave motion during heavy storm is seemed to be quite significant to lateral response of the structure.

• Journal of the Society of Disaster Information
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• v.11 no.1
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• pp.89-96
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• 2015

To reduce noise inside an elevated railroad station, the vibration of the station structure should be lowered, and to establish more effective anti-vibration measures it is required that, reflecting the track-bridge-structure interaction, the transfer characteristics of vibration induced by a train is well identified. In this paper, the current status of domestic railroad stations is classified, and the vibration transfer characteristics is analyzed via measurement data from representative elevated stations. From the analysis results on the measurement data, in transferring vibration from the track to the structure, remarkable vibration reduction in higher frequency range is observed, and, in some stations, amplified response characteristics in lower frequency range is identified. Also, for stations with floating track system or TPS type, relatively greater reduction in transferred vibration is observed.

• Biomolecules & Therapeutics
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• v.25 no.6
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• pp.593-598
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• 2017

The $Na^+/H^+$ exchanger-1 (NHE-1) is a ubiquitously expressed pH-regulatory membrane protein that functions in the brain, heart, and other organs. It is increased by intracellular acidosis through the interaction of intracellular $H^+$ with an allosteric modifier site in the transport domain. In the previous study, we reported that glutamate-induced NHE-1 phosphorylation mediated by activation of protein kinase C-${\beta}$ (PKC-${\beta}$) in cultured neuron cells via extracellular signal-regulated kinases (ERK)/p90 ribosomal s6 kinases (p90RSK) pathway results in NHE-1 activation. However, whether glutamate stimulates NHE-1 activity solely by the allosteric mechanism remains elusive. Cultured primary cortical neuronal cells were subjected to intracellular acidosis by exposure to $100{\mu}M$ glutamate or 20 mM $NH_4Cl$. After the desired duration of intracellular acidosis, the phosphorylation and activation of PKC-${\beta}$, ERK1/2 and p90RSK were determined by Western blotting. We investigated whether the duration of intracellular acidosis is controlled by glutamate exposure time. The NHE-1 activation increased while intracellular acidosis sustained for >3 min. To determine if sustained intracellular acidosis induced NHE-1 phosphorylation, we examined phosphorylation of NHE-1 induced by intracellular acidosis by transient exposure to $NH_4Cl$. Sustained intracellular acidosis led to activation and phosphorylation of NHE-1. In addition, sustained intracellular acidosis also activated the PKC-${\beta}$, ERK1/2, and p90RSK in neuronal cells. We conclude that glutamate stimulates NHE-1 activity through sustained intracellular acidosis, which mediates NHE-1 phosphorylation regulated by PKC-${\beta}$/ERK1/2/p90RSK pathway in neuronal cells.

• Journal of the Korean Society of Visualization
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• v.18 no.2
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• pp.59-65
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• 2020

Vertical axis wind energy systems including 3 and 4 blades are numerically investigated in a two-dimensional (2D) computational domain. The power coefficient (C_p) is adopted to measure the efficiency of the system and the effect of the rotating velocity on the power coefficient is analyzed for the two different systems. The rotating velocity varies from 30 rad/s to 90 rad/s, which corresponds to the tip speed ratio (T.S.R) of 0.5 to 1.5. The torque exerted on the blades is mainly determined by the aerodynamic force in the x-direction and maximized when the blade is positioned at around θ = 186°. The efficiency of the 4-blade system is higher than that of the 3-blade system within the tip speed ratio range between 0.5 and 0.67, besides where the 3-blade system shows a better performance. For the 3-blade system, the maximum efficiency is reached to 0.082 at the tip speed ratio of 1.083. The maximum efficiency of the 4-blade system is 0.071 at T.S.R. = 0.92. The velocity fields in the x-direction, pressure fields, and the vorticity magnitude are analyzed in detail for the optimal cases of the 3- and 4-blades systems, respectively.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.21 no.5
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• pp.410-418
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• 2009

Based on a linear potential theory, the boundary element method(BEM) is developed and applied to analyze the hydrodynamic forces and the motion responses of a floating body with a partially reflective sidewall. The hydrodynamic forces (added mass and damping coefficients) are dependent on not only the submergence of a floating body and the reflection of a sidewall, but also the gap between body and sidewall. In particular, the partial reflection of a sidewall plays an importance role in the motion responses of a floating body at resonant frequencies. It reduces the resonant peaks caused by resonance phenomenon due to the wave trapping in an enclosed fluid domain between body and sidewall. Developed predictive tools can be used to assess the motion performance of a floating body for various combinations of configuration of a floating body, wave heading, sidewall properties, and wave characteristics and applied to supply the basic informations for the harbour design considering the motion characteristics of a moored ship.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.3
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• pp.232-241
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• 2011

The hybrid rocket shows interesting characteristics of complicated mixing layer developed by the interaction between turbulent oxidizer flow and injected surface mass flow from fuel vaporization. In this study, the compressible LES was conducted to explore the physical phenomena of surface oscillatory flow induced by the flow interferences in a duct domain. From the numerical results, the wall injection generates the stronger streamwise vorticites and the negative components of axial velocity accompanied with the azimuthal vorticity near the surface. And the vortex shedding with a certain time scale was found to be developed by hydrodynamic instability in the mixing layer. The pressure fluctuations in this calculation exhibit a peculiar peak at a specific angular frequency($\omega$=8.8) representing intrinsic oscillation due to the injection.

• Journal of the Korean Geotechnical Society
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• v.34 no.7
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• pp.51-58
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• 2018

In this study a series of centrifuge tests were carried out in dry sand to analyze the comparison of lateral pile behavior for static loading and dynamic loading condition. In case of static loading condition, the lateral displacement was applied up to 50% of pile diameter by deflection control method. And the input sine wave of 0.1 g~0.4 g amplitude and 1 Hz frequency was applied at the base of the soil box using shaking table for dynamic loading condition. From comparison of experimental static p-y curve obtained from static loading tests with API p-y curves, API p-y curves can predict well within 20% error the ultimate subgrade reaction force of static loading condition. The ultimate subgrade reaction force of experimental dynamic p-y curve is 5 times larger than that of API p-y curves and experimental static p-y curves. Therefore, pseudo-static analysis applied to existing p-y curve for seismic design could greatly underestimate the soil resistance at non-linear domain and cause overly conservative design.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2011.04a
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• pp.531-538
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• 2011

To ensure the structural integrity of membrane type LNG tank, the rational assessment of impact pressure and structural responses due to sloshing should be preceded. The sloshing impact pressures acting on the insulation system of LNG tank are typical irregular loads and the structural responses caused by them also very complex behaviors including fluid structure interaction. So it is not easy to estimate them accurately and huge time consuming process would be necessary. In this research, a simplified method to analyze the dynamic structural responses of LNG tank insulation system under pressure time histories obtained by sloshing model test or numerical analysis was proposed. This technique basically based on the concept of linear combination of the triangular response functions which are obtained by the transient response analysis under the unit triangular impact pressure acting on structures in time domain. The validity of suggested method was verified through the example calculations and applied to the structural analysis of real Mark III type insulation system using the sloshing impact pressure time histories obtained by model test.

• Bulletin of the Korean Chemical Society
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• v.28 no.9
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• pp.1499-1509
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• 2007

Proteomic analyses of synaptic vesicle fraction from rat brain have been performed for the better understanding of vesicle regulation and signal transmission. Two different approaches were applied to identify proteins in synaptic vesicle fraction. First, the isolated synaptic vesicle proteins were treated with trypsin, and the resulting peptides were analyzed using a high-pressure capillary reversed phase liquid chromatography/tandem mass spectrometry (cRPLC/MS/MS). Alternatively, proteins were separated by two-dimensional gel electrophoresis (2DE) and identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS). Total 18 and 52 proteins were identified from cRPLC/MS-MS and 2DE-MALDI-TOF-MS analysis, respectively. Among them only 2 proteins were identified by both methods. Of the proteins identified, 70% were soluble proteins and 30% were membrane proteins. They were categorized by their functions in vesicle trafficking and biogenesis, energy metabolism, signal transduction, transport and unknown functions. Among them, 27 proteins were not previously reported as synaptic proteins. The cellular functions of unknown proteins were estimated from the analysis of domain structure, expression profile and predicted interaction partners.