• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.78-78
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• 2002

The role of heat shock proteins in shielding organism from environmental stress is illustrated by the large-scale synthesis of these protein by the organism studied to date. However, recent evidence also suggests an important role for heat shock protein in fertilization and early development of mammalian embryos. Effects of elevated in vitro temperature on in vitro produced bovine embryos were analysed in order to determine its impact on the expression of heat shock protein 70 (HSP70) by control and frozen-thawed after in vitro fertilization (IVF) or nuclear transfer (NT). The objective of this study was to assess the developmental potential in vitro produced embryos with using of the various containers and examined expression and localization of heat shock protein 70 after it's frozen -thawed. For the vitrification, in vitro produced embryos at 2 cell, 8 cell and blastocysts stage after IVF and NT were exposed the ethylene glycol 5.5 M freezing solution (EG 5.5) for 30 sec, loaded on each containers such EM grid, straw and cryo-loop and then immediately plunged into liquid nitrogen. Thawed embryos were serially diluted in sucrose solution, each for 1 min, and cultured in CRI-aa medium. Survival rates of the vitrification production were assessed by re-expanded, hatched blastocysts. There were no differences in the survival rates of IVF using EM grid, cryo-loop. However, survival rates by straw were relatively lower than other containers. Only, nuclear transferred embryos survived by using cryo-loop. After IVF or NT, in vitro matured bovine embryos 2 cell, 8 cell and blastocysts subjected to control and thawed conditions were analysed by semiquantitive reverse transcription polymerase chain reaction methods for hsp 70 mRNA expression. Results revealed the expression of hsp 70 mRNA were higher thawed embryos than control embryos. Immunocytochemistry used to localization the hsp70 protein in embryos. Two, 8-cell embryos derived under control condition was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed frozen-thawed. However, under control condition, blastocysts displayed aggregate signal while Hsp70 in frozen-thawed blastocysts appeared to be more uniform in distribution.

• Journal of the Korean Geotechnical Society
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• v.23 no.11
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• pp.87-98
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• 2007

For the quick rehabilitation of a fire-damaged tunnel structure, it is the most important procedure to investigate the fire-induced damaged zone rapidly. This study aims to propose a new drilling resistance testing method by which mechanical properties of tunnel concrete lining altered by high temperature can be estimated easily and continuously. Especially, it alms to derive the relationships to estimate mechanical properties of mortar and concrete materials from drilling parameters. To obtain the optimum testing condition, a series of drilling resistance tests were carried out for mortar specimens. When the rotation per minute of drill bit, tile penetration rate and the bit diameter were 1,300 rpm, 1.40 mm/sec, and 10 mm respectively, the deviation of measured drilling resistance forces was minimal. Under the optimum testing condition, the relationships between drilling resistance and mechanical properties of mortar specimens were shown to be very favorable. The concept of replacing a mean value of resistance farces measured during drilling with the resistance energy was proposed to consider the effects of randomly distributed aggregates inside a concrete material on drilling resistance. When the concept was applied to concrete materials, a favorable relationship between actual compressive strength and drilling resistance energy was also successfully derived.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2B
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• pp.107-120
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• 2010

The effect of potential future climate change on the inflow of agricultural reservoir and its impact to downstream streamflow by reservoir operation for paddy irrigation water was assessed using the SLURP (semi-distributed land use-based runoff process), a physically based hydrological model. The fundamental input data (elevation, meteorological data, land use, soil, vegetation) was collected to calibrate and validate of the SLURP model for a 366.5 $km^2$ watershed including two agricultural reservoirs (Geumgwang and Gosam) located in Anseongcheon watershed. Then, the CCCma CGCM2 data by SRES (special report on emissions scenarios) A2 and B2 scenarios of the IPCC (intergovernmental panel on climate change) was used to assess the future potential climate change. The future weather data for the year, m ms, m5ms and 2amms was downscaled by Change Factor method through bias-correction using 3m years (1977-2006) weather data of 3 meteorological stations of the watershed. In addition, the future land uses were predicted by modified CA (cellular automata)-Markov technique using the time series land use data fromFactosat images. Also the future vegetation cover information was predicted and considered by the linear regression between monthly NDVI (normalized difference vegetation index) from NOAA AVHRR images and monthly mean temperature using eight years (1998-2006) data.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.2B
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• pp.215-224
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• 2008

The effect of streamflow considering future land use change and vegetation index information by climate change scenario was assessed using SLURP (Semi-distributed Land-Use Runoff Process) model. The model was calibrated and verified using 4 years (1999-2002) daily observed streamflow data for the upstream watershed ($260.4km^2$) of Gyeongan water level gauging station. By applying CA-Markov technique, the future land uses (2030, 2060, 2090) were predicted after test the comparison of 2004 Landsat land use and 2004 CA-Markov land use by 1996 and 2000 land use data. The future land use showed a tendency that the forest and paddy decreased while urban, grassland and bareground increased. The future vegetation indices (2030, 2060, 2090) were estimated by the equation of linear regression between monthly NDVI of NOAA AVHRR images and monthly mean temperature of 5 years (1998-2002). Using CCCma CGCM2 simulation result based on SRES A2 and B2 scenario (2030s, 2060s, 2090s) of IPCC and data were downscaled by Stochastic Spatio-Temporal Random Cascade Model (SST-RCM) technique, the model showed that the future runoff ratio was predicted from 13% to 34% while the runoff ratio of 1999-2002 was 59%. On the other hand, the impact on runoff ratio by land use change showed about 0.1% to 1% increase.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.253-262
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• 2023

This study compared the results of observing the early life history of Gymnogobius urotaenia, which lives in Hwangbocheon Stream, an inflow stream on the east coast, with the differences between fish of the same Gobiidae fishes. In May 2022 and 2023, fertilized eggs and brood stork scattered under rocks were captured twice in Hwangbocheon Stream. The spawning amount was 827~1,540 and the orchid was a elliptical in shape with a size of 3.21×1.07 mm. The stage of ovulation observed in the laboratory was 16 cells, and hatching began after 193 hours. The breeding water temperature range was 18.8~19.3℃. Newly after hatching larvae, the yolk sac with a total length of 3.84~4.33 (average 4.10±0.17, n=30) mm, and the anus was not open. 6 days after hatching of incubation, the total length was 5.32~6.11 (average 5.67±0.25, n=30) mm, absorbing all egg yolk and transitioning to the preflexion larvae, ingesting food, and developing a keynote on the tail fin. 15 days after hatching, the end of the urostyle end was completely bent at 45° with a total length of 7.33~8.52 (average 7.81±0.46, n=30) mm and transitioned to postflexion larvae, and melanophore developed throughout the body. 38 days after hatching, the total length is 22.1~26.1 (23.8±1.36, n=30) mm and the number of fins (6 first dorsal fins, 11 second dorsal fins, and 11 anal fins, 12 ventral fins) is all the number of fin base became integer and transferred to the juvenile. As a result of the study, it was possible to distinguish the melanophore of postflexion larvae of G. urotaenia from other postflexion larvae Gobiidae fish in that they were distributed throughout the body of half the body and tail.

• Korean Journal of Plant Resources
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• v.37 no.2
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• pp.137-148
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• 2024

The purpose of this study is to investigate the weather, soil characteristics, and location environment of Pimpinella brachycarpa natural habitats in order to gather the essential information for the conservation of these habitats. P. brachycarpa are distributed throughout Korea and are mainly found to grow in shady and humid areas between 500 and 1,200 m above sea level. The average annual temperature in Mt. Duta was 13.1℃, and the average annual precipitation in Mt. Jungwon was 1,509 mm, which was higher than in other regions. The pH ranged from 4.42 to 4.97, indicating slight acidity. The total N content ranged from 0.18% to 0.68%, and the available P ranged from 13.43 to 531.56 mg/kg, demonstrating notable regional variations. The species diversity index (H') was highest at Mt. Ilwol, measuring 1.713. The evenness (J') ranged from 0.983 to 0.993, and the dominance (D') ranged from 0.007 to 0.017. The similarity index was very low, averaging 24.86%, and it was divided into communities of Wilson's elm (Ulmus davidiana var. japonica) and communities of Korean maple (Acer pseudo-siebodianum).

• Journal of the Korean Society of Marine Environment & Safety
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• v.15 no.3
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• pp.187-203
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• 2009

This study monitored marine environments in system coastal waters of the South Sea of Korea during the period of 2005-2007 and analyzed the data from environmental parameters and nutrients, which contributed to fluctuate marine environmental characteristics in aquaculture. On the basis of the fluctuation of temperature depending on seasons and sampling sites, too seasons of winter and summer showed a remarkable temperature gap of $5^{\circ}C$ compared with spring and autumn. Salinity in spring and summer approached 9 psu in Morkpo. Most of sampling sites showed COD below 2mg/L, indicating somewhat optimal environmental conditions. T-N and T-P also had desirable horizontal distributions all year round the concentration of SS was higher, but Chi. a was well-distributed horizontally except for Morkpo. Marone environmental parameters of surface water were similar to those of bottome water except for Morkpo. Most of sampling sites showed the ratio of N/P below 16, impling that nitrogen played an important role in growth of phytoplankton as a limiting factor. The relationship between Chi. a and nutrients was positive linear, whereas the relationship between Chi. a and COD were very strong positive. winder, Spring and Summer showed the strong relationship between Chi. a and COD. In the analysis of dendogram based on environmental parameters using SPSS ver. 10.0, the station 7 did not belong to other stations, and kept an independent place. The relationships of waters between Jindo, Wando and Heanam ware close, compared with other waters. Consequently, these regions except for Morkpo into which fresh water is introduced by the dike showed somewhat optimal environmental parameters and nutrients in year, and were assumed to maintain the desirable marine conditions for aquaculture.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.2
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• pp.105-116
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• 2000

In order to investigate the seasonal variation of watermass in the central coast of the southern sea of korea, oceanographic observation on the fishing grounds were carried out by the trainingship of Yosu University on May, Aug. and Nov. in 1998 and Feb. in 1999. The resultes obtained are summerized as follows : 1). The watermass in the fishing ground were divided into the coastal water(30.0~31.6$\textperthousand$ ), mixing water(31.7~33.4$\textperthousand$) and the offshore water(33.5~35.0$\textperthousand$) according to the distribution of salinity from T-S diagram plotted all salinity data observed on May, Aug. and Nov. in 1998 and Feb. in 1999. 2) The ranges of temperature and salinity were from 14.1$^{\circ}C$ to 18.8$^{\circ}C$ and from 32.2$\textperthousand$ to 34.9$\textperthousand$ in spring(May), from 14.2$^{\circ}C$ to 27.7$^{\circ}C$ and from 29.0$\textperthousand$ to 34.7$\textperthousand$ in summer(August), from 13.4$^{\circ}C$ to 21.3$^{\circ}C$ and from 31.45$\textperthousand$ to 34.5$\textperthousand$ in autumn(November) and from 8.2$^{\circ}C$ to 14.8$^{\circ}C$ and from 33.9$\textperthousand$ to 34.6$\textperthousand$ in winter(February), respectively. 3) The distribution of watermass in the fishing ground varied largely each seasons, but a general tendency on the distribution was obtained. That is, in spring and autumm the offshore water was distributed most widely and in summer the coastal and mixing water occupied the fishing ground but in winter the offshore water prevailed. 4) Variation of temperature and salinity were appeared between the surface and 30m in the coastal region and between the surface and 50m in the open ocaen region. Therefore, in the summer the thermocline and halocline were made between surface and 30m layer with vertical gradients of 10.5$^{\circ}C$/30m and 4.0$\textperthousand$/30m in the coastal region and in the open ocean region the thermocline and halocline were made between surface and 50m layer with vertical gradients of 13.$0^{\circ}C$/50m and 3.8$\textperthousand$/50m.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.140-147
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• 1985

This experiment was conducted in the field to investigate the effects of mulched condition on nutrient's mineralization and its mobility as affected by environmental changes (soil moisture and soil temperature) in the plow layer. 1. Nitrogen mineralization was faster owing to the prevention of evaporation and the increase of soil temperature in the poly ethylene mulched plot. As a result, nitrate form of nitrogen was much earlier available for plant in mulched plot than in non mulched plot. 2. Available $P_2O_5$ content in the plow layer was kept highest in the fertilized zone (10-20 cm depth) regardless of difference between mulched and non mulched condition, and was nearly constant without showing difference between planting and non planting of tobacco due to the lower availability of phosphorus by tobacco. 3. Potassium applied in the plow layer was remained a large quantity until the latter growth stage resulting in the lower recovery of potassium by tobacco. 4. Mineralized nutrients such as $NH_4-N$, $NO_3-N$, and K in the plow layer of mulched plot were transported from the fertilized layer (10-20 cm depth) and accumulated to the surface layer (0-10 cm depth) as the growing time goes by, but those in non mulched plot were distributed to the deeper layer. 5. Mobility of available nutrients in the plow layer was If order as $NO_3-N$ $$\geq_-$$ $NH_4-N$ > K > available $P_2O_5$. 6. Leaching degree of mineralized nutrients in the mulched plot was much lower than in the non mulched plot.

• Journal of Environmental Policy
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• v.9 no.2
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• pp.157-184
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• 2010

Global climate change is destroying the water circulation balance by changing rates of precipitation, recharge and discharge, and evapotranspiration. The Intergovernmental Panel on Climate Change (IPCC 2007) makes "changes in rainfall pattern due to climate system changes and consequent shortage of available water resource" a high priority as the weakest part among the effects of human environment caused by future climate changes. Groundwater, which occupies a considerable portion of the world's water resources, is related to climate change via surface water such as rivers, lakes, and marshes, and "direct" interactions, being indirectly affected through recharge. Therefore, in order to quantify the effects of climate change on groundwater resources, it is necessary to not only predict the main variables of climate change but to also accurately predict the underground rainfall recharge quantity. In this paper, the authors selected a relevant climate change scenario, In this context, the authors selected A1B from the Special Report on Emission Scenario (SRES) which is distributed at Korea Meteorological Administration. By using data on temperature, rainfall, soil, and land use, the groundwater recharge rate for the research area was estimated by period and embodied as geographic information system (GIS). In order to calculate the groundwater recharge quantity, Visual HELP3 was used as main model for groundwater recharge, and the physical properties of weather, temperature, and soil layers were used as main input data. General changes to water circulation due to climate change have already been predicted. In order to systematically solve problems associated with how the groundwater resource circulation system should be reflected in future policies pertaining to groundwater resources, it may be urgent to recalculate the groundwater recharge quantity and consequent quantity for using via prediction of climate change in Korea in the future and then reflection of the results. The space-time calculation of changes to the groundwater recharge quantity in the study area may serve as a foundation to present additional measures for the improved management of domestic groundwater resources.