• Journal of ILASS-Korea
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• v.9 no.3
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• pp.22-28
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• 2004

This study is concerned about the characteristics of ultrasonic-energy-added W/O type emulsified fuel. The distilled water was mixed with diesel oil by using ultrasonic energy fuel feeding system and then the SMD of sprayed droplets was measured to find out atomization characteristics of emulsified fuel by using the Malvern 2600 system. The capacitance value was measured to verify stability of the same specimen by using the digital LCR meter, EDC1630 additionally. The main results are as follows; 1) The more measuring distance increases between one hole nozzle tip and analyser bearm, the more SMD increases. 2) The more water content increases, the more capacitance value increases depending on the time. Main Parameters of the study are the amount of water content $0{\sim}30%$ by 5% in emulsified fuel, and the measurement distance, $20{\sim}140mm$ by 10mm or 20mm between nozzle tip and analyser beam.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.286-297
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• 1994

The optimum nutritional and cultural conditions of polygalacturonase by Ganoderma lucidum in liquid culture were studied. The optimal temperature, pH, and the duration of culture for production of the enzyme was $30^{\circ}C$, 5.5 and 14 days, respectively. The maximal production of the enzyme was obtained in a synthetic medium containing 10 g of pectin, 10 g of soluble starch, 1 g of yeast extract, 2 g of peptone, 1 g of phenylalanine, 2 g of $KH_2PO_4$, 0.2 g of $MgSO_4{\cdot}7H_2O$, 0.05 g of $CaCI_2$ and 100 g of $thiamin{\cdot}HCI$ in 1000 ml of distilled water.

• Journal of Biosystems Engineering
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• v.31 no.5 s.118
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• pp.443-448
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• 2006

For development of an on-line monitoring unit of fermentation process, an auto dilution unit based on traditional chemical and biological analytical method was developed and the performance was evaluated. The dilution unit was constructed with two syringe pumps and flow direction change valves and fully automated. Total delivery volume of two pumps using distilled water was measured to confirm the operating stability And diluted concentrations of three substrate solutions (glucose, lactic acid, ethanol) were compared with a standard method with a high performance liquid chromatograph (glucose, lactic acid) and gas chromatograph (ethanol). Relative error values of total delivery volume of the pumps were below 3% and standard deviation values were 0.003 (n=5). Relative error values of diluted concentration of the dilution unit measurements were below 2% with 1/10 of dilution ratio and 70, $80{\mu}{\ell}$ of sample volume conditions for glucose and lactic acid, 1/30 of dilution ratio and $70{\mu}{\ell}$ of sample volume conditions for ethanol, respectively. In case of the ethanol, cause of the evaporative characteristics, the relative error values showed over 5% whole experimental conditions.

• Korean Journal of Pharmacognosy
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• v.46 no.4
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• pp.342-351
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• 2015

In this study, we performed quantitative determination of the three marker compounds such as gallic acid, ellagic acid, and quercetin in the 70% ethanol extracts of non-processed Sanguisorbae Radix and processed Sanguisorbae Radix using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the three compounds was used a Gemini $C_{18}$ column ($5{\mu}m$, $4.6{\times}250mm$) by the gradient elution with distilled water and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate and injection volume were $1.0{\mu}L/min$ and $10{\mu}L$. The concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix were 0.25, 0.26, and 0.007%, respectively, while the concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix 0.14-0.55, 0.27-2.03, and 0.001-0.007%, respectively. Among the three components, the amount of the ellagic acid was increased after processing in Sanguisorbae Radix.

• Journal of the Korean Society of Clothing and Textiles
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• v.8 no.1
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• pp.39-45
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• 1984

The effects of Temperature on the removal of triglyceride were studied with soaps having various chain lengths of alkyl group. Cellophane, polyester film and alkali-treated polyester film were soiled with tripalmitin, tagged with 0" and detergency was evaluated by analysing the tripalmitine on the fabric before and after washing by means of liquid scintillation counting. The results were as following: 1) Triglyceride was completely removed from cellophane in distilled water without surfactant at any temperature, because of the hydrophillic nature of cellulose. The detergency of triglyceride from polyester film fully depended on the state of tripalmitin. The detergency of alkali treated polyester film was better than that of untreated polyester film at lower temperature due to increased hydrophillcity, but worse at higher temperature due to the diffusion of molten tripalmitin into the grooves, formed by alkali treatment. 2) The detergency from polyester film was increased with elevating temperature and after reaching some optimum detergencies, the detergencies were rather decreased with increasing temperature. The temperatures of optimum detergency were shifted to higher with increasing chain length of alkyl group. 3) When the soiled film was baked at $60^{\circ}C$ and $70^{\circ}C$ for 20 min, the detergency vs. temperature was much the same as the case of without-baking. These results indicate that the detergency of triglyceride was largely correlated with the suspending power of surfactants at low temperature and with state of soil and hydrophilicity of substrates with elevating temperature.

• Geophysics and Geophysical Exploration
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• v.7 no.1
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• pp.25-32
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• 2004

We have carried out laboratory measurements of P-wave velocity and deformation strain during $CO_2$ injection into a porous sandstone sample, in dry and water-saturated conditions. The rock sample was cylindrical, with the axis normal to the bedding plane, and fluid injection was performed from one end. Using a piezoelectric transducer array system, we mapped fluid movement during injection of distilled water into dry sandstone, and of gaseous, liquid, and supercritical $CO_2$ into a water-saturated sample. The velocity changes caused by water injection ranged from $5.61\;to\;7.52\%$. The velocity changes caused by $CO_2$ injection are typically about $-6\%$, and about $-10\%$ for injection of supercritical $CO_2$, Such changes in velocity show that the seismic method may be useful in mapping $CO_2$ movement in the subsurface. Strain normal to the bedding plane was greater than strain parallel to the bedding plane during $CO_2$ injection; injection of supercritical $CO_2$ showed a particularly strong effect. Strain changes suggest the possibility of monitoring rock mass deformation by using borehole tiltmeters at geological sequestration sites. We also found differences associated with $CO_2$ phases in velocity and strain changes during injection.

• Microbiology and Biotechnology Letters
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• v.15 no.2
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• pp.89-94
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• 1987

The optimal conditions for the protoplast formation and regeneration of Streptomyces mitakaensis have been investigated. S. mitakaensis cells were converted to protoplast by treating with 0.1 mg/$m\ell$ of lysozyme in phosphate-tris buffer (pH 7.2) to the cells grown at the late logarithmic growth phase in the GBYN medium (gycerol 20g, beef extract 5g, yeast extract 5g, NaCl 5g in 1 liter of distilled water) contained 0.5% glycine. Cell regeneration from protoplast was accomplished in 10 days post inoculation on the R2 regeneration agar medium and at 3 days post inoculation on the H2 regeneration liquid medium. The efficiency of the regeneration was 0.l% in 3 days at 35$^{\circ}C$.

• Journal of Life Science
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• v.20 no.5
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• pp.782-788
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• 2010

The protective effects of a powder mixed with solid-cultured and liquid-cultured Lentinus edodes mycelia (2 : 1, w/w) (designate LED) with different doses of carbon tetrachloride ($CCl_4$) on induced hepatotoxicity in male Sprague-Dawley (SD) rats was investigated. The rats were divided into seven groups (6 rats/group) and the following substances were administered orally to each group: Vehicle (0.2 ml distilled water), Control (0.2 ml distilled water), LED (LED 100, 200, 300 and 400 mg/kg BW in 0.2 ml distilled water), and Silymarin (200 mg/Kg BW in 0.2 ml distilled water). After two weeks of daily administration, all groups except for the Vehiclegroup were subjected to abdominal injection with $CCl_4$ ($CCl_4$ : corn oil, 1 : 1 v/v; 0.5 ml/kg BW). One day later, blood and liver samples were collected to analyze biomarkers. All LED treatments elevated hepatic superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH peroxidase) activities, and reduced thiobarbituric reactive substances (TBARS), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and interleukin-6 (IL-6), resulting in the reduction of glutamate-oxalate transaminase (GOT), glutamate-pyruvate transaminase (GPT) and lactic acid dehydrogenase (LDH) activities in plasma. These results indicate that LED effectively protected SD rat hepatotoxicity induced by $CCl_4$ through its antioxidative activity and reduction of some cytokines. The highest efficacy was found in LED 200 mg/kg BW, showing potential as a useful material for protection from hepatotoxicity in humans.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.593-597
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• 2004

This study was conducted to investigate the quality characteristics of liquid calcium acetate (LCAs) and solid calcium acetate (SCAs), Br-LCA, Pe-LCA and Ap-LCA as liquid form, Br-SCA, Pe-SCA and Ap-SCA as solid form. Calcium acetate was prepared by reacting of vinegars [brown rice (Br), persimmons (Pe) and apple (Ap)] and ash of black snail in order to obtain natural water soluble calcium resources. The pHs of the vinegars for preparing calcium acetate (CA) were the range of 2.34 ∼ 3.06, and the contents of the ash of black snail which reacted to 100 mL of the vinegars were 20.43∼23.50 g. The yields of solid CAs from 100 mL of the vinegars were 11.02∼13.01 g. The colors of liquid and solid CAs were light yellow in Ap-LCA and Ap-SCA, brown in Br-LCA and Br-SCA, dark brown in Pe-LCA and Pe-SCA. Calcium contents of Br-LCA, Pe-LCA and Ap-LCA were 3.02, 2.06 and 2.30% (w/v), and those of Br-SCA, Pe-SCA and Ap-SCA were 27.15, 16.31 and 19.48% (w/w), respectively. The solubilities of the solid CAs were 36.82 ∼ 39.92% (w/v) in distilled water, 32.05 ∼ 39.04% (w/v) in Soju, 13.12 ∼ 18.65% (w/v) in thick soysauce, 38.35 ∼ 38.90% (w/v) in ionic beverage, 33.47 ∼ 35.58% (w/v) in yoghurt, while the solid CAs formed the curds in soymilk and milk. The sour and bitter taste of the CAs were lower, while the astringent taste, fishy flavor and savory taste were higher than those of standard CA.

• Korean Journal of Environmental Agriculture
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• v.33 no.3
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• pp.164-168
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• 2014

BACKGROUND: Increase in application of liquid pig manure(LPM) in agriculture as nutritional source has become a social issue due to its influence on water quality. Also, proper application methods have not been developed with respect to indigenous properties of LPM and soil physical properties. Therefore, we conducted this experiment to observe the infiltration characteristics and distribution of dissolved organic compounds of LPM in soils having different soil textures. METHODS AND RESULTS: To do this experiment, we collected three different soils and LPM. We analyzed the physical and chemical properties of both soils and LPM to determine the dilution ratios of LPM. The LPM diluted to 4 different ratios with distilled water was applied to the top of soil column. Infiltration rates were observed by time and depth until the amount of effluent collected from the bottom of the soil columns were stabilized while maintaining the hydraulic head 3 cm above the soil column. The results showed that infiltration rates increased with increasing dilution ratios in the order of sandy, loamy sand, and sandy loam. The time required to reach steady state was increased with decreasing sand contents clay. CONCLUSION: The size and amount of the dissolved organic compounds in LPM that can determine the efficiency as fertilizer and environmental problems as nonpoint pollution source in water quality have not been investigated with respect to behavior and transport of them in soil. Therefore, it requires further research how we can properly apply LPM as valuable fertilizer substitute for inorganic fertilizers.