• 제목/요약/키워드: Direct seeding method

검색결과 112건 처리시간 0.028초

벼 건답직파재배답(乾畓直播栽培畓)에서의 잡초발생(雜草發生) 및 효과적(效果的)인 방제체계(防除體系) (Weed Growth and Effective Control in Direct-seeded Rice Fields)

  • 최충돈;문병철;김순철;오윤진
    • 한국잡초학회지
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    • 제15권3호
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    • pp.175-182
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    • 1995
  • 벼 건답직파(乾짧直播) 재배답(栽培畓)에서의 잡초발생(雜草發生) 양상(樣相)을 구명(究明)하고 효과적(效果的)인 제초체계(除草體系) 확립(確立)을 위하여 1994년도에 영남농업시험장(領南農業試驗場)에서 수행(遂行)한 일련(一連)의 시험결과(試驗結果)를 요약(要約)하면 다음과 같다. 1. 벼 단작(單作)의 1모작답(毛作畓)에 비하여 미맥(米麥) 2모작답(毛作畓)에서는 피를 비롯한 대부분의 잡초(雜草) 발생(發生)이 경감(輕減)되었고, 전체적(全體的)으로 약 30%정도(程度)의 잡초발생(雜草發生) 경감효과(輕減效果)가 있었으며, 벼수량(收量)도 증수(增收)되는 경향(傾向)이었다. 2. 성력화(省力化)를 위한 무경운(無耕耘) 재배(栽培)는 앵미와 이형주(異型株)의 발생(發生)을 증가(增加)시킬 뿐만 아니라 피와 독새풀의 발생(發生)도 급증(急增)하였다. 3. 피와 바랭이는 온도(溫度)가 낮을수록, 종자(種子)가 깊이 묻힐수록 출아(出芽)가 늦었으며, 동일(同一)한 조건(條件)(온도(溫度), 종자매몰심도(種子埋沒深度))에서 피는 바랭이 에비하여 3-5일(日) 출아(出芽)가 빨랐다. 출아율(出芽率)도 온도(溫度)와 매몰심도(埋沒深度)에 영향(影響)을 받았는데, 피는 온도(溫度)의 영향(影響)이 큰 반면(反面) 바랭이는 매몰심도(埋沒深度)의 영향(影響)이 컸다. 4. 수량성(收量性) 확보(確保)를 전제로한 바람직한 제초체계(除草體系)는 건답상태(乾畓狀態)(벼 출아기전후(出芽期前後))에서 1회처리(回處理)+담수상태(湛水狀態) l회(回)의 체계처리(體系處理)로서, 전(全) 생육기간(生育期間)에 걸쳐 90%정도(程度)의 제초효과(除草效果)를 나타내었으며, 수량성(收量性)도 손제초구(除草區) 대비(對比)90%를 상회(上廻)하였다.

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자연적인 자운영 재입모를 위한 적정 벼 재배유형 (Rice Cropping Methods for Natural Reestablishment of Chinese Milkvetch)

  • 김영광;홍광표;정완규;최용조;송근우;강진호
    • 한국작물학회지
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    • 제46권6호
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    • pp.473-477
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    • 2001
  • 벼-자운영의 연속재배체계 확립을 통해 자운영을 지속적으로 이용하고자 기계이앙 및 직파 파종시기와 재배양식을 달리하여 시험한 결과는 다음과 같다. 1. 자운영의 식물체 무기성분함량은 T-N이 2.92%, P$_2$ $O_{5}$가 0.74%, $K_2$O가 4.28%로 ㏊당 30 ton정도의 녹비가 생산되면 벼 재배시 인산을 제외하고는 표준시비량 이상에 해당되었다. 2. 자운영 녹비의 포장 잔존율은 초기 1주일 이내에 급격히 떨어져 토양에 환원되었고, 무기성분의 잔존율도 같은 경향이었다. 질소성분에 비해 인산과 가리성분의 환원정도가 더 빨랐고, 심토에 비해 표토에서 낮은 결과를 보였다. 3. 무경운 직파재배에서는 5월 25일 이후 파종할 경우 파종 시기에 관계없이 벼 수확후 자연적인 자운영 재입모로 다음해 피복이 가능하였다. 4. 무경운이앙은 자운영의 지상부 예취 없이는 벼 이앙이 불가능하여 농가활용이 곤란하였고, 경운이앙은 5월 25일 이앙시 벼 수확후 자운영의 자연적인 입모가 극히 불량한 반면, 6월 4일 경운후 이앙시 벼의 충분한 생육과 수량이 확보되고 운영의 자연적인 재입모로 다음해에도 자운영 조성이 가능하였다. 5. 자운영포장에 벼 이앙시 결주율은 경운에 비해 무경운에서 높았고 이앙심도도 무경운이앙시 예취된 자운성 때문에 얕아 도복이 우려되었다. 6. 자운영 후작 포장의 벼 수량은 무경운직파의 경우 입모가 불량하여 수량이 낮았으나, 경운이앙의 경우 자운생이 재배되지 않은 대조구에 비해 4~6% 증수되었다.

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코이어 배지의 포수 및 정식 방법에 따른 수경재배 오이의 생육 및 과실 특성 (Growth and Fruit Characteristics according to Filling and Planting Methods of Coir Medium Hydroponically Grown Cucumber)

  • 이흥수;배효준;배종향;고바울
    • 생물환경조절학회지
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    • 제33권1호
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    • pp.37-44
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    • 2024
  • 본 연구는 코이어 배지를 이용한 오이 수경재배에서 배지 포수액의 종류와 정식 방법에 따른 생육 및 생산성을 검증하여 가장 효율성이 높은 재배 방법의 선발을 위하여 수행되었다. 포수액은 배양액(S)과 원수(W), 육묘는 암면큐브 육묘(RC), 암면 플러그묘(RP), 슬라브 직파(DS)로 하였다. 재배 기간을 동일하게 하기 위하여 기준일은 파종일로 하였다. 초기 생육량은 S 처리에 서 유의하게 높았으나, 생육기간이 길어질수록 처리 간 차이가 감소하였다. 정식 방법과 생육량 간에는 관계성이 나타나지 않았으나, 포수 방법에 따라 동일한 정식방법 간에도 다른 결과가 나타났다. 생산성도 동일한 경향으로 초기 수확기인 파종 후 6-8주까지는 S 처리에서 수확과의 수가 유의하게많았으며, 이후 동일한 기간 수량의 격차가 해소되었다. 누적 수량은 초기 수확량의 차이로 인하여 S 처리에서 유의하게 높았다. 식물체의 생육과 과실 생산성은 유사한 경향으로 선형적인 관계성을 나타내었다. 정식 방법 간에는 상관성을 나타내지 않았으나, S의 DS 처리가 초기 근권부 안정적인 양·수분 공급으로 초기 생육 및 수량이 가장 많은 경향이었다. 따라서 S 처리한 배지에 DS 방식이 식물체의 생육 및 생산성에 가장 유리할 뿐만 아니라 재배단계 간소화를 통한 노동력 및 생산 원가 절감으로 경제성 측면에서도 기여할 수 있을 것으로 생각된다.

생쥐 2세포기배의 동결보존 (Cryopreservation of Mouse 2-Cell Embryos)

  • 백청순;서병희;이재현;이경광
    • Clinical and Experimental Reproductive Medicine
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    • 제16권1호
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    • pp.9-14
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    • 1989
  • For the cryopreservation of human embryos this study was accomplished as a preliminary experiment. The purpose of this study is to obtain optimal cryoprotectant, addition and dilution method of cryoprotectant and cooling rate for raising survival of frozon and thawed 2-cell mouse embryos. Seeding was done at $-7^{\circ}C$ and the straw contained embryos was plunged at $-30^{\circ}C$ when the slow cooling was ended. Embryos those developed normally to blastocyst after in vitro culture for over 96 hours were regarded as survival ones. The survival was the rate of number of survival embryos against the recovered embryos. The results are followed : 1. The survivals were 6.3, 71.2 and 67.4% respectively, when Glycerol, DMSO and 1,2-Propanediol were used as cryoprotectant. 2. When sucrose was added in freezing solution, the survival was 69.0%. That was higher than the survival of embryos frozen without sucrose in freezing solution. The difference was not significant. 3. Addition and dilution of cryoprotectant by 4 stepwise raised the survival than by direct, but that was not significant. 4. When embryos were frozen by -0.3, -0.5 and $-1^{\circ}C/min$ before plunged into $LN_2$, the survivals were 67.9, 78.0 and 37.0% respectively. The differnce was significant.

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Mapping quantitative trait loci controlling low-temperature germinability in rice

  • Nguyen, Hoang Nam;Park, In-Kyu;Yeo, Sang-Min;Yun, Yeo-Tae;Ahn, Sang-Nag
    • 농업과학연구
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    • 제39권4호
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    • pp.477-482
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    • 2012
  • Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at $15^{\circ}C$ was measured to represent low-temperature germination and used for QTL analysis. The germination rate at $15^{\circ}C$ for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.

Comparison of yield and its components in spring sown wheat and barley by path coefficient analysis

  • Choi, In-Bae;Kim, Hak-Sin;Hwang, Jae-Bok;Bae, Hui-Su;Ku, Bon-Il;Park, Hong-Kyu;Par, Tae-Seon;Lee, Geon-Hwi
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.234-234
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    • 2017
  • Recent abnormal weather, especially continued rainfall during sowing season causes difficulty in proper sowing of wheat and delayed sowing after November 15 is concerned about freezing damage during winter, resulting in reduction of wheat yield. To correspond government policy of crop sufficiency improvement and produce and supply raw wheat and barley steadily, expansion of cultivation area is necessary and spring sowing of wheat is required. To obtain basic information on the improvement of spring sown wheat and barley production, comparison and path coefficients analysis was conducted for yield and yield related components from autumn and spring sown wheat and barley. Path analyses were known as very useful in clarifying the effects of yield components on grain yield formation, which were not accurately reflected in simple correlation anaylses. Most cultivated 5 wheat and 9 barley cultivars were sown on October and February at Cheon-ju province according to standard sowing method. For the spring sowing of wheat and barley, the varieties having vernalization degree I~III are seeded in the mid of February and seeding rate is 200~250kg/ha which is increased by 25% than autumn sowing. N-fertilizer of 95 kg/ha and the same amount of P, K dressed in autumn are applied at once as basal fertilizer. The magnitude of direct effect in each yield components on yield was in sequence as follows. In autumn wheat, grain number per $spike{\geq}$ the number of spike per $m^2$>1000-grain weight and in spring wheat, grain number per $ spike{\geq}the$ number of spike per $m^2$> 1000-grain weight. In autumn naked barley, 1000-grain weight> the number of spike per $m^2$, grain number per spike and in spring barely, the number of spike per $m^2$> grain number per spike > 1000-grain weight. In autumn covered barley, grain number per spike>the number of spike per $m^2$ and in spring coverd barley, the number of spike per $m^2$> grain number per spike, 1000-grain weight. In autumn malt barley, the number of spike per $m^2$>1000-grain weight and in spring malt barley, the direct effects of three yield components were similar. According to the path analysis of yield components for spring sown wheat and barley, it was suggested that adequate number of spike per $m^2$ was most important factor for yield increase.

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벼 무경운 재배시 재배양식에 따른 생육 및 수량 (Rice Growth and Yield at Different Cultural Methods under No-tillage Condition)

  • 박홍규;김상수;백남현;석순종;박건호;이선용
    • 한국작물학회지
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    • 제41권4호
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    • pp.420-428
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    • 1996
  • 획기적인 벼 생산비 절감 기술 확립을 위해1993~1995년 호남농업시험장인 전북통(미사질양토)에서 동진벼를 공시하고 무경운 기계이앙, 무경운 무논골뿌림, 무경운 표면조파, 무경운 산파, 경운 기계이앙 등 5가지로 하여 재배 양식간의 생육 및 수량성의 차이를 비교 검토한 결과는 다음과 같다. 1. 토양 경도는 무경운에서 높았고(토심 10cm의 경도 : 무경운 1.6kg/$cm^2$, 경운 1.0kg/$cm^2$), 토괴 심도가 깊을수록 경운과의 차이가 커지는 경향이었으며, 토양 물리성중 가비중은 무경운이 경운보다 높았으며 공극률은 경운에서 58.5~55.9로 무경운의 52.1~50.5보다 높았다. 2. 무경운 직파재배시 m$^2$당 입모수는 l13~133개였으며 이앙재배시 결주율은 경운 이앙 3%, 무경운 이앙 4.5%로 무경운 이앙에서 높았고, 유효경 비율은 경운 이앙이 높았으며, 무경운 재배 양식간에는 이앙>무논골뿌림>표면조파>산파의 순으로 높았다. 3. 잡초 발생량은 무경운에서 많았으며 경운에서는 피, 올양개, 방동사니가 우점하였고 무경운에서는 나도겨풀, 사마귀풀, 둑새풀이 우점하였 다. 4. 뿌리량은 경운 이앙에서 가장 많았고, 무경운재배 양식간에는 이앙>무논골뿌림>표면조파>산파의 순으로 많았으며, 뿌리 분포는 무경운에서 경운보다 표층 분포가 많았다. 특히 표면에 파종한 산파와 표면조파에서 표층 분포가많았다. 5. 하위 절간의 분포 비율은 도복이 심했던 무경운 표면조파와 산파에서 낮았고, 무경운 무논골뿌림, 무경운 이앙은 경운 이앙과 비슷하였으며, 중심고는 이앙재배에 비해 직파재배에서낮았고 줄기 매몰 심도는 경운(3.1cm)에 비해 무경운에서 낮았는데 무경운 표면조파, 산파에서는 0.6~0.7cm로 현저히 낮았다. 6. 도장 도복은 무경운재배시 산파>표면조파>무논골뿌림>이앙재배 순으로 높게 발생했으며, 특히 무경운 표면조파와 산파에서 뿌리도복이 심하게 발생되었다. 7. m$^2$당 수수는 경운 이아이 가장 많았고 무경운산파가 가장 적었으며, 등숙 비율은 포장도복이 심했던 무경운 표면조파, 산파에서 가장 낮았다. 벼 수량성은 경운, 무경운 이앙은 서로 비슷하였고 무경운 무논골뿌림은 경운 이앙의 약 93%였으며, 무경운 표면조파, 산파는 포장도복으로 크게 낮아 경운 이질의 87~81%정도였다.

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주요(主要) 채소용(菜蔬用) 제초제(除草劑)의 토양중(土壤中)에서의 잔효(殘效)와 후작물(後作物)에 미치는 영향(影響) - 제(第)1보(報) 월동작물(越冬作物)에 처리(處理)한 제초제(除草劑)의 잔효(殘效)와 후작물(後作物)에의 영향(影響) - (Residual Activity and Effect of Soil Applied Herbicides on Succeeding Crops in Vegetable Field - 1. Residual Activity and Effect of Applied Herbicides on Succeeding Crops in Winter Crops -)

  • 양환승;문영희;최은석;장민수;이진하
    • 한국잡초학회지
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    • 제11권1호
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    • pp.32-49
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    • 1991
  • 주요(主要) 채소용(菜蔬用) 제초제(除草劑)의 잔효기간(殘效期間)과 후작물(後作物)에 미치는 영향(影響)을 조사(調査)코자 월동작물(越冬作物) 5종(種)(가을배추, 무, 시금치, 양파, 마늘)을 포장(圃場)에 파종(播種)하고 각 작물(作物)에 적용(適用)이 가능시된 제초제(除草劑)를 약량별(藥量別)로 처리(處理)한후 생물검정(生物檢定)에 의하여 경시적으로 조사(調査)하였다. 1. 처리(處理)된 제초제(除草劑)의 잔효기간(殘效其間)과 후작물(後作物)에 대한 약해유무(藥害有無)(carry-over injury)는 공시토양(供試土壤)의 종류(種類)나 재배작물(栽培作物)의 종류(種類)간에는 큰 차이가 없었다. 그러나 제초제(除草劑)의 처리약량(處理藥量), 토양(土壤)의 채취심도(採取深度), 검정식물(檢定植物)의 종류(種類) 및 접종일자(接種日字)(경과일수(經過日數)) 등에 따라 차이가 뚜렷하였다. 그러나 잔효성제초제(殘效性除草劑)라도 후작물(後作物)의 종류(種類)(감수성작물(感受性作物) 피함), 약제처리후(藥劑處理後) 후작물(後作物)의 접종일자(接種日字) 연장, 경운심도(耕耘深度) 등의 조절로 후작물약해(後作物藥害)는 최소화 할 수 있었다. 2. 월동작물(越冬作物)에 처리된 제초제중(除草劑中) 그 작기(作期)가 종료시 (동작(冬作) 200-240일)까지 잔효(殘效)가 거의 남지 않아 후작물(後作物)에 안전(安全)한 제초제(除草劑)는 alachlor, trifluralin, prometry 등이었다. 3. Pendimethalin, metolachlor, linuron, methabenzthiazuron 등은 추천약량(推薦藥量)까지는 작기종료(作期終了)와 동시에 안전하나 배량처리(倍量處理)가 될때에는 그 약제(藥劑)에 감수성작물(感受性作物)의 생육(生育)에는 영향(影響)이 있었다. 4. Napropamide는 동작(冬作)에 처리(處理)시 150-300ga.i/10a 약량(藥量) 처리시(處理時)에는 작기 종료후(終了後)에도 후작물(後作物) 중 화본과(禾本科)인 IR, 직파벼, 보리등에는 영향(影響)이 있었으나 담수하(湛水下) 이앙(移秧)벼에 대한 영향(影響)은 없었고, 십자화과, 호로과, 가지과작물(作物) 등에도 영향(影響)이 없었다. 5. 대표적으로 잔효(殘效)가 긴 nitralin은 동작(冬作)에서는 275일후(日後)에도 화본과(禾本科)인 IR, 벼, 보리, 담수하(湛水下) 이앙(移秧)벼에는 75ga.i./10a약량(藥量)에서도 생육억제(生育抑制)가 있었고, 그이외 참깨, 들깨, 시금치 등의 생육(生育)에도 약간의 영향(影響)이 있었으나 십자화과, 호로과, 가지과 등에 대한 영향(影響)은 적었다.

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불량환경하(不良環境下)에서의 제초제(除草劑) 약해(藥害)와 경감기술(輕減技術) (Herbicidal Phytotoxicity under Adverse Environments and Countermeasures)

  • 권용웅;황형식;강병화
    • 한국잡초학회지
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    • 제13권4호
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    • pp.210-233
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    • 1993
  • The herbicide has become indispensable as much as nitrogen fertilizer in Korean agriculture from 1970 onwards. It is estimated that in 1991 more than 40 herbicides were registered for rice crop and treated to an area 1.41 times the rice acreage ; more than 30 herbicides were registered for field crops and treated to 89% of the crop area ; the treatment acreage of 3 non-selective foliar-applied herbicides reached 2,555 thousand hectares. During the last 25 years herbicides have benefited the Korean farmers substantially in labor, cost and time of farming. Any herbicide which causes crop injury in ordinary uses is not allowed to register in most country. Herbicides, however, can cause crop injury more or less when they are misused, abused or used under adverse environments. The herbicide use more than 100% of crop acreage means an increased probability of which herbicides are used wrong or under adverse situation. This is true as evidenced by that about 25% of farmers have experienced the herbicide caused crop injury more than once during last 10 years on authors' nationwide surveys in 1992 and 1993 ; one-half of the injury incidences were with crop yield loss greater than 10%. Crop injury caused by herbicide had not occurred to a serious extent in the 1960s when the herbicides fewer than 5 were used by farmers to the field less than 12% of total acreage. Farmers ascribed about 53% of the herbicidal injury incidences at their fields to their misuses such as overdose, careless or improper application, off-time application or wrong choice of the herbicide, etc. While 47% of the incidences were mainly due to adverse natural conditions. Such misuses can be reduced to a minimum through enhanced education/extension services for right uses and, although undesirable, increased farmers' experiences of phytotoxicity. The most difficult primary problem arises from lack of countermeasures for farmers to cope with various adverse environmental conditions. At present almost all the herbicides have"Do not use!" instructions on label to avoid crop injury under adverse environments. These "Do not use!" situations Include sandy, highly percolating, or infertile soils, cool water gushing paddy, poorly draining paddy, terraced paddy, too wet or dry soils, days of abnormally cool or high air temperature, etc. Meanwhile, the cultivated lands are under poor conditions : the average organic matter content ranges 2.5 to 2.8% in paddy soil and 2.0 to 2.6% in upland soil ; the canon exchange capacity ranges 8 to 12 m.e. ; approximately 43% of paddy and 56% of upland are of sandy to sandy gravel soil ; only 42% of paddy and 16% of upland fields are on flat land. The present situation would mean that about 40 to 50% of soil applied herbicides are used on the field where the label instructs "Do not use!". Yet no positive effort has been made for 25 years long by government or companies to develop countermeasures. It is a really sophisticated social problem. In the 1960s and 1970s a subside program to incoporate hillside red clayish soil into sandy paddy as well as campaign for increased application of compost to the field had been operating. Yet majority of the sandy soils remains sandy and the program and campaign had been stopped. With regard to this sandy soil problem the authors have developed a method of "split application of a herbicide onto sandy soil field". A model case study has been carried out with success and is introduced with key procedure in this paper. Climate is variable in its nature. Among the climatic components sudden fall or rise in temperature is hardly avoidable for a crop plant. Our spring air temperature fluctuates so much ; for example, the daily mean air temperature of Inchon city varied from 6.31 to $16.81^{\circ}C$ on April 20, early seeding time of crops, within${\times}$2Sd range of 30 year records. Seeding early in season means an increased liability to phytotoxicity, and this will be more evident in direct water-seeding of rice. About 20% of farmers depend on the cold underground-water pumped for rice irrigation. If the well is deep over 70m, the fresh water may be about $10^{\circ}C$ cold. The water should be warmed to about $20^{\circ}C$ before irrigation. This is not so practiced well by farmers. In addition to the forementioned adverse conditions there exist many other aspects to be amended. Among them the worst for liquid spray type herbicides is almost total lacking in proper knowledge of nozzle types and concern with even spray by the administrative, rural extension officers, company and farmers. Even not available in the market are the nozzles and sprayers appropriate for herbicides spray. Most people perceive all the pesticide sprayers same and concern much with the speed and easiness of spray, not with correct spray. There exist many points to be improved to minimize herbicidal phytotoxicity in Korea and many ways to achieve the goal. First of all it is suggested that 1) the present evaluation of a new herbicide at standard and double doses in registration trials is to be an evaluation for standard, double and triple doses to exploit the response slope in making decision for approval and recommendation of different dose for different situation on label, 2) the government is to recognize the facts and nature of the present problem to correct the present misperceptions and to develop an appropriate national program for improvement of soil conditions, spray equipment, extention manpower and services, 3) the researchers are to enhance researches on the countermeasures and 4) the herbicide makers/dealers are to correct their misperceptions and policy for sales, to develop database on the detailed use conditions of consumer one by one and to serve the consumers with direct counsel based on the database.

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삼차원 배양된 슈반세포 도관을 이용한 말초 신경 재생 (PERIPHERAL NERVE REGENERATION USING A THREE-DIMENSIONALLY CULTURED SCHWANN CELL CONDUIT)

  • 김성민;이종호
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제30권1호
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    • pp.1-16
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    • 2004
  • The use of artificial nerve conduit containing viable Schwann cells is one of the most promising strategies to repair the peripheral nerve injury. To fabricate an effective nerve conduit whose microstructure and internal environment are more favorable in the nerve regeneration than existing ones, a new three-dimensional Schwann cell culture technique using $Matrigel^{(R)}$. and dorsal root ganglion (DRG) was developed. Nerve conduit of three-dimensionally arranged Schwann cells was fabricated using direct seeding of freshly harvested DRG into a $Matrigel^{(R)}$ filled silicone tube (I.D. 1.98 mm, 14 mm length) and in vitro rafting culture for 2 weeks. The nerve regeneration efficacy of three-dimensionally cultured Schwann cell conduit (3D conduit group, n=6) was assessed using SD rat sciatic nerve defect of 10 mm, and compared with that of silicone conduit filled with $Matrigel^{(R)}$ and Schwann cells prepared from the conventional plain culture method (2D conduit group, n=6). After 12 weeks, sciatic function was evaluated with sciatic function index (SFI) and gait analysis, and histomorphology of nerve conduit and the innervated tissues of sciatic nerve were examined using image analyzer and electromicroscopic methods. The SFI and ankle stance angle (ASA) in the functional evaluation were $-60.1{\pm}13.9$, $37.9^{\circ}{\pm}5.4^{\circ}$ in 3D conduit group (n=5) and $-87.0{\pm}12.9$, $32.2^{\circ}{\pm}4.8^{\circ}$ in 2D conduit group (n=4), respectively. And the myelinated axon was $44.91%{\pm}0.13%$ in 3D conduit group and $13.05%{\pm}1.95%$ in 2D conduit group to the sham group. In the TEM study, 3D conduit group showed more abundant myelinated nerve fibers with well organized and thickened extracellular collagen than 2D conduit group, and gastrocnemius muscle and biceps femoris tendon in 3D conduit group were less atrophied and showed decreased fibrosis with less fatty infiltration than 2D conduit group. In conclusion, new three-dimensional Schwann cell culture technique was established, and nerve conduit fabricated using this technique showed much improved nerve regeneration capacity than the silicone tube filled with $Matrigel^{(R)}$ and Schwann cells prepared from the conventional plain culture method.