• The Transactions of the Korean Institute of Power Electronics
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• v.6 no.3
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• pp.258-264
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• 2001

In this paper, a new control strategy of a series active power filter suing direct compensating voltage extraction method is proposed. The proposed series active power filter and shunt passive filters are used 3-phase 3-wire power system with nonlinear load. The series active power filter complements drawbacks of the shunt passive filter and contributes to a source side harmonic reduction. We can extract the compensating voltage of the series active power filter using performance function without phase transformations. Therefore, the calculating time is short and the control method is simple compared with conventional methods. Experimental results verify that the system using the proposed method appears a good performance.

• Mycobiology
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• v.38 no.1
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• pp.33-38
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• 2010

We have successfully applied the nested PCR to detect Cylindrocarpon destructans, a major pathogen causing root rot disease from ginseng seedlings in our former study. The PCR assay, in this study, was used to detect the pathogen from soils. The nested PCR using internal transcribed spacer (ITS) 1, 4 primer set and Dest 1, 4 primer set maintained the specificity in soils containing various microorganisms. For a soil DNA extraction method targeting chlamydospores, when several cell wall disrupting methods were tested, the combination of lyophilization and grinding with glass beads, which broke almost all the chlamydospores, was the strongest. The DNA extraction method which was completed based on the above was simple and time-saving because of exclusion of unnecessary stages, and efficient to apply in soils. As three ginseng fields whose histories were known were analyzed, the PCR assay resulted as our expectation derived from the field information. The direct PCR method will be utilized as a reliable and rapid tool for detecting and monitoring C. destructans in ginseng fields.

• Korean Journal of Remote Sensing
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• v.19 no.5
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• pp.393-402
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• 2003

In times of the civil uses of commercialized high-resolution satellite imagery, applications of remote sensing have been widely extended to the new fields or the problem solving beyond traditional application domains. Transportation application of this sensor data, related to the automatic or semiautomatic road extraction, is regarded as one of the important issues in uses of remote sensing imagery. Related to these trends, this study focuses on automatic road extraction using Gradient Direction Profile Algorithm (GDPA) scheme, with IKONOS panchromatic imagery having 1 meter resolution. For this, the GDPA scheme and its main modules were reviewed with processing steps and implemented as a prototype software. Using the extracted bi-level image and ground truth coming from actual GIS layer, overall accuracy evaluation and ranking error-assessment were performed. As the processed results, road information can be automatically extracted; by the way, it is pointed out that some user-defined variables should be carefully determined in using high-resolution satellite imagery in the dense or low contrast areas. While, the GDPA method needs additional processing, because direct results using this method do not produce high overall accuracy or ranking value. The main advantage of the GDPA scheme on road features extraction can be noted as its performance and further applicability. This experiment study can be extended into practical application fields related to remote sensing.

• Mass Spectrometry Letters
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• v.10 no.2
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• pp.66-70
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• 2019

Rivaroxaban (RRN) is the first available active direct factor Xa inhibitor (anticoagulant) with oral administration. Due to its success in market, there have been efforts to develop various RRN formulations, and the development of good analytical methods for its in vivo evaluation is an essential prerequisite. Thus, here, a simple and efficient method to determine RRN in rat plasma using liquid-liquid extraction (LLE) and liquid chromatography and multiple reaction monitoring (LC-MRM) was presented. The use of ethyl acetate as the LLE solvent results appropriate extraction and purification of RRN and it also helps the significant reduction of rat plasma volume required for RRN quantitation. The developed method showed good analytical performance including specificity, linearity ($r^2{\geq}0.999$ within 0.5 - 500 ng/mL), sensitivity (the lower limit of quantitation at 0.5 ng/mL), accuracy (89.3 - 107.0%), precision (${\geq}12.7%$), and recovery (89.2 - 105.7%). Additionally, RRN in sample extracts showed good stability. Finally, the applicability of the validated method to the PK evaluation of RRN was confirmed after its oral administration to normal rats. The present method is the first analytical method employing LLE for the simple and efficient extraction and purification of RRN in rat plasma. Therefore, the present method can contribute to the development of new RRN formulations as well as to the monitoring of RRN in special clinical situations through its efficient determination in various samples with or without minor modification.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.12 no.3
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• pp.256-262
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• 2012

Direct teaching of the industrial robot is a novel technique to easily teach manipulators. However, teaching data by human hand cannot help having large noise errors ranging from low to high frequency. To use teaching data, post-processing to correct the teaching trajectory is required. Here, a novel shape-based trajectory correction method to rebuild teaching data with the feature information of curvature and velocity is proposed. The proposed method is tested on square and circular objects.

• Journal of the Semiconductor & Display Technology
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• v.15 no.4
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• pp.97-102
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• 2016

Image stitching is a process of combining two or more images with overlapping area to create a panorama of input images, which is considered as an active research area in computer vision, especially in the field of augmented reality with 360 degree images. Image stitching techniques can be categorized into two general approaches: direct and feature based techniques. Direct techniques compare all the pixel intensities of the images with each other, while feature based approaches aim to determine a relationship between the images through distinct features extracted from the images. This paper proposes a novel image stitching method based on feature pixels with approximated clustering filter. When the features are extracted from input images, we calculate a meaning of the minutiae, and apply an effective feature extraction algorithm to improve the processing time. With the evaluation of the results, the proposed method is corresponding accurate and effective, compared to the previous approaches.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.5
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• pp.638-648
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• 2004

In many drinking water treatment plants, chlorination process is one of the main techniques used for the disinfection of water. This disinfecting treatment leads to the formation of haloacetic acid (HAAs). In this study, headspace solid-phase microextraction (HS-SPME) was studied as a possible alternative to liquid-liquid extraction for the analysis of HAAs in drinking water. The method involves direct derivatization of the acids to their methyl esters without methyl tert-butyl ether (MTBE) extraction, followed by HS-SPME with a $2cm-50/30{\mu}m$ divinylbenzene/carboxen/polydimethylsiloxane fiber. The effects of experimental parameters such as selection of SPME fiber, the volume of sulphuric acid and methanol, derivatization temperature and time, the addition of salts, extraction temperature and time, and desorption time on the analysis were investigated. Analytical parameters such as linearity, repeatability and limit of detection were also evaluated. The $2cm-50/30{\mu}m$-divinylbenzene/carboxen/polydimethylsiloxane fiber, sulphuric acid of 1ml, methanol of 3ml, derivatization temperature of $50^{\circ}C$ derivatization time of 2hrs, sodium chloride salt of 10g, extraction time of 30 minutes, extraction temperature of $20^{\circ}C$ and desorption time of 1 minute at $260^{\circ}C$ were selected as the optimal experimental conditions for the analysis of HAAs. The linearities ($r^2$), relative standard deviations (%RSD) and limits of detection (LOD) for HAAs were 0.9978~0.9991, 1.1~9.8% and $0.05{\sim}0.2{\mu}g/l$, respectively.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.51 no.3
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• pp.108-111
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• 2002

This paper proposes an new water mark embedding and extraction technique which extends the direct sequence spread spectrum technique. The proposed technique approximates the complexity of image and block in spatial domain using Laplacian filtering and watermark is adaptively embedded in the mid-frequency DCT components. Local parity bits are attached to higher-frequency DCT components and they are used to detect extraction errors and correct those errors. In extraction process the proposed method boosts the higher frequency components of image and extracts the watermark by demodulation and this information is verified and adjusted by parity bits. Experimental results show it is invisible and robust to several external attacks.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1593-1601
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• 2017

Vibrio species are generally recognized as pathogens predominant in seafood along coastal areas. The food industry has sought to develop efficient microbial detection methods. Owing to the limits of conventional methods, this study aimed to establish a rapid identification method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were compared to determine the appropriate means to pretreat Vibrio species, using 17 isolates and five reference strains. Extended direct transfer and full formic acid extraction methods using bacterial colonies on agar plates revealed very low identification rates. Formic acid and trifluoroacetic acid (TFA) extractions using bacterial broth cultures were also performed. All Vibrio isolates and reference strains prepared by TFA extraction were successfully identified to the species level (17/22, 77.3%) and to the genus level (5/22, 22.7%). Thus, TFA extraction was considered the most appropriate method to pretreat Vibrio species for MALDI-TOF MS. The remaining 33 isolates and two reference strains were prepared by TFA extraction and analyzed by MALDI-TOF MS. Overall, 50 isolates were identified to the species level (40/50, 80%) and to the genus level (10/50, 20%). All isolates were identified as 43 V. alginolyticus, six V. parahaemolyticus, and one V. vulnificus species. V. alginolyticus and V. parahaemolyticus were isolated from fish offal (87.5% and 12.5%, respectively), seawater (91.3%, 8.7%), and shellfish (62.5%, 37.5%), whereas V. alginolyticus and V. vulnificus were isolated from sediment (90.9% and 9.1%, respectively). This study established a reliable system of MALDI-TOF MS preparation and analysis for Vibrio identification.

• Korean Journal of Pharmacognosy
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• v.41 no.1
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• pp.62-66
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• 2010

In result of analysis for the content determination of ursolic acid(UA) and oleanolic acid(OA) in extract of Eriobotryae folium using HPLC with UV detector, UA in chloroform extract of Moo-mok variety was showed highest content(2.7843 mg/g). And OA in ethyl acetate extract of Dae-bang variety was showed highest content(0.5898 mg/g). These result suggest that direct extraction using organic solvent(chloroform or ethyl acetate) was useful method for rapid quantitative analysis of UA and OA without preprocessing such as drying or fractionation.