• Applied Microscopy
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• v.51
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• pp.2.1-2.7
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• 2021

Synaptic vesicles, which are endogenous to neurotransmitters, are involved in exocytosis by active potentials and release neurotransmitters. Synaptic vesicles used in neurotransmitter release are reused via endocytosis to maintain a pool of synaptic vesicles. Synaptic vesicles show different types of exo- and endocytosis depending on animal species, type of nerve cell, and electrical activity. To accurately understand the dynamics of synaptic vesicles, direct observation of synaptic vesicles is required; however, it was difficult to observe synaptic vesicles of size 40-50 nm in living neurons. The exo-and endocytosis of synaptic vesicles was confirmed by labeling the vesicles with a fluorescent agent and measuring the changes in fluorescence intensity. To date, various methods of labeling synaptic vesicles have been proposed, and each method has its own characteristics, strength, and drawbacks. In this study, we introduce methods that can measure presynaptic activity and describe the characteristics of each technique.

• Korean Journal of Optics and Photonics
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• v.26 no.3
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• pp.147-154
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• 2015

We propose a method for simultaneously measuring the front and back surface profiles of transparent optical components. The proposed method combines dual-wavelength transmission deflectometry with liquids to record distorted phases at different wavelengths, and then numerically reconstructs the three-dimensional phase information to image the front and back surfaces of the lens. We propose a theoretical model to determine the surface information, and a bifocal lens is experimentally investigated. Unlike conventional transmission deflectometry, our proposed method supports direct observation of the front and back surface profiles of the optical elements.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.9 s.240
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• pp.1225-1234
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• 2005

Mode I stress intensity factor $(K_I)$ of Notched Ring Test(NRT) specimen for measuring slow crack growth resistance was found using finite-element method. The theoretical $K_I$ value of NRT was not available in any references and could not be solved analytically. At first, in order to verify the accuracy of the finite-element approach, published $K_I$ values of several cracks were calculated and compared with finite-element results. The results were in good agreement within inherent errors of theoretical $K_I$. Finally the mode I stress intensity factor of NRT was found using 2- and 3-dimensional finite-element methods and expressed as a function of the applied load. This enabled direct comparison of resistance to slow crack growth between NRT and Notched Pipe Test(NPT), which employ different loading regime.

• Korean Journal of Computational Design and Engineering
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• v.10 no.2
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• pp.114-120
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• 2005

This paper presents a novel method for radius measurement of fillet regions of polygonal models by using optimum onhogonal planes. The objective function for finding an optimum onhogonal plane is designed based on the orthogonality between the normal vectors of the faces in a filet region and the plane that is to be found. Direct search methods are employed to solve the defined optimization problem since no explicit derivatives of the object function can be calculated. Once an optimum orthogonal plane is obtained, the intersection between the onhogonal plane and the faces of interest is calculated, and necessary point data in the fillet region for measuring radii are extracted by some manipulation. Then, the radius of the fillet region in question is measured by least squares fitting of a circle to the extracted point data. The proposed radius measuring method could eliminate the burden of defining a plane for radius measurement, and automatically find a necessary optimum orthogonal plane. It has an advantage in that it can measure fillet radii without prior complicated segmentation of fillet regions and explicit information of neighboring surfaces. The proposed method is demonstrated trough some mea-surement examples.

• Journal of Korean Society of Transportation
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• v.28 no.3
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• pp.157-167
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• 2010

Density is the most important congestion indicator among the three fundamental flow variables, flow, speed and density. Measuring density in the field has two different ways, direct and indirect. Taking photos with wide views is one of direct ways, which is not widely used because of its cost and lacking of proper positions. Another direct density measuring method using two point detectors has been introduced with the concept of instantaneous density, average density and measurement interval. The relationship between accuracy and measurement interval has been investigated using the SIMULATION data produced by Paramics Application Programming Interface function. We analyze the affect of segment density accuracy by sensing gap each road condition such as sensing segment length, lane and LOS after gathering data by Paramics Application Programming Interface.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.8 no.1
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• pp.37-44
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• 2009

Density is the most important congestion indicator among the three fundamental flow variables, flow, speed and density. Measuring density in the field has two different ways, direct and indirect. Taking photos with wide views is one of direct ways, which is not widely used because of its cost and lacking of proper positions. Another direct density measuring method using two spot detectors has been introduced with the concept of instantaneous density, average density and measurement interval. The relationship between accuracy and measurement interval has been investigated using the simulation data produced by Paramics API function. Finally, density measurement algorithm has been suggested including exponential smoothing for device development.

• Journal of Korean Society of Disaster and Security
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• v.13 no.4
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• pp.65-74
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• 2020

Continuous and accurate instrument of river water usage is needed for sustainable river water management. Although the instrument methods applicable to each point of use of river water are different, more precise direct instrument methods are required at the point of major open channel. Users of river water should select appropriate direct instrument methods to measure usage, but there is a lack of standards and verification research. In this study, the H-Q rating curve method, ultrasonic method, and microwave method were applied directly to the test basin in the upper basin of Mangyeong river, and the accuracy of measurement data was evaluated by comparing absolute error between discharge data calculated by instrument method. When comparing the calculated discharge of point units, the ultrasonic method showed the best results of the actual measurement. Through continuous instrument, the sum of the daily and monthly units was compared, and the ultrasonic and microwave methods were shown to be highly accurate. Based on the results of this study, it is hoped that the appropriate direct measurement method can be selected according to the importance of the river water use facility, considering that the ultrasonic method and the microwave method are relatively costly compared to the water level-flow relationship method.

• 한국초전도학회:학술대회논문집
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• v.9
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• pp.161-164
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• 1999

We have fabricated the direct-coupled planar type 1st-order SQUID gradiometers. The gradiometer consists of moats or slots in SQUID loop. It is made by YBa$_2Cu_3O_7$ thin films using pulsed laser deposition method on SrTiO$_3$ single crystal and bi-crystal substrates. We have studied the effects of slots and moats in SQUID loop by measuring the voltage modulation signals under uniform field and 1st-order gradient, and the noise properties under non-shielded environment.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.597-600
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• 2003

The development and maintenance of a sound bond are an essential requirements of concrete repair and replacement. The bond property of a bonded overlay to its substrate concrete during the lifetime is one of the most important performance requirements which should be quantified. A standard or a verified bond strength measurement method is required at field for screening, selecting materials and quality control for overlay or repair materials. This study compares the nipple pipe direct tensile test, flexural adhesion test, and core pull-off test with their test results. Substantial differences in the failure stresses of three test methods were attributed to their different geometries and loading conditions. From these comparison and investigation, core pull-off test was relatively good because the coefficient of variation values were about 2%. It would be suitable for use in-situ because of its simplicity and accuracy.

• Journal of Korean Society of Rural Planning
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• v.13 no.4
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• pp.1-8
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• 2007

The dichotomous-choice contingent valuation method is applied to estimate the landscape value of Jeju Island's stonewall fencing farming land. A distribution-free approach, Turnbull empirical distribution model, is employed to solve negative willingness to pay and truncation problems. The data used are collected from the interviews with tourist about willingness-to-pay at the various donation amount levels of Jeju stonewall's landscape value. The evaluation result is shown that the average amount of willingness to pay for the Jeju stonewall fencing farming land is 3,001 won per meter with standard deviation of 511.0 won/m.