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Analysis of the Gene Expression by Laser Capture Microdissection (III) -Microarray Analysis of the Gene Expression at the Mouse Uterine Luminal Epithelium of the Implantation Sites during Apposition Period- (Laser Capture Microdissection을 이용한 유전자 발현 연구 (III) -생쥐 착상 부위 자궁 내강상피 조직에서 배아 병치 기간 동안 일어나는 유전자 발현에 관한 Microarray 분석-)

  • Yoon, Se-Jin;Jeon, Eun-Hyun;Park, Chang-Eun;Ko, Jung-Jae;Choi, Dong-Hee;Cha, Kwang-Yul;Kim, Se-Nyun;Lee, Kyung-Ah
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.4
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    • pp.323-335
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    • 2002
  • Object: The present study was accomplished to obtain a gene expression profile of the luminal epithelium during embryo apposition in comparison of implantation (1M) and interimplantation (INTER) sites. Material and Method: The mouse uterine luminal epithelium from IM and INTER sites were sampled on day 4.5 (Day of vaginal plug = day 0.5) by Laser Captured Microdissection (LCM). RNA was extracted from LCM captured epithelium, amplified, labeled and hybridized to microarrays. Results from microarray hybridization were analyzed by Significance Analysis of Microarrays (SAM) method. Differential expression of some genes was confirmed by LCM followed by RT-PCR. Results: Comparison of IM and INTER sites by SAM identified 73 genes most highly ranked at IM, while 13 genes at the INTER sites, within the estimated false discovery rate (FDR) of 0.163. Among 73 genes at IM, 20 were EST/unknown function, and the remain 53 were categorized to the structural, cell cycle, gene/protein expression, immune reaction, invasion, metabolism, oxidative stress, and signal transduction. Of the 24 structural genes, 14 were related especially to extracellular matrix and tissue remodeling. Meanwhile, among 13 genes up-regulated at INTER, 8 genes were EST/unknown function, and the rest 5 were related to metabolism, signal transduction, and gene/protein expression. Among these 58 (53+5) genes with known functions, 13 genes (22.4%) were related with $Ca^{2+}$ for their function. Conclusions: Results of the present study suggest that 1) active tissue remodeling is occurring at the IM sites during embryo apposition, 2) the INTER sites are relatively quiescent than IM sites, and 3) the $Ca^{2+}$ may be a crucial for apposition. Search for human homologue of those genes expressed in the mouse luminal epithelium during apposition will help to understand the implantation process and/or implantation failure in humans.

A Study on the Current Status and Policy Direction of Open Banking (오픈뱅킹(Open Banking)의 현황과 정책방향에 관한 연구)

  • Park, Jeongkuk;Kim, Injai
    • Journal of Service Research and Studies
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    • v.10 no.1
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    • pp.17-31
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    • 2020
  • Open banking, the global trend of the financial industry, is the driving force behind various innovations in the financial market in the future. The right policy direction and detailed tasks are important for triggering the differentiation and reunion of the financial industry. The purpose of this study is to investigate and analyze the background of open banking, domestic and international trends, and Korea's open banking policy. The policy directions and tasks for successful settlement and activation of open banking system are carefully suggested. Open banking is a policy to allow third party provider(TPP) access to bank accounts and open payment functions under the explicit consent of the customer. The opening of the open banking era is expected to begin competition and cooperation between banks and fintech companies in earnest, thus enhancing the competitiveness of the financial industry and contributing to the utility of financial consumers. To this end, policymakers should make every effort to advance open-ended financial settlement infrastructure, open banking legal grounds, and minimize side effects such as customer data leakage and poor financial system stability. Banks and fintech companies will need to focus on scattered customer financial information on a single platform and develop it into a convergence and discrimination of true financial services.

Optical Design of the Integrated Triplexer Fabricated by Micro Block Stacking Method (MBS 방법으로 제작한 집적형 Triplexer의 광학 설계)

  • Yoon, Hyun-Jae;Kim, Jong-Hyuk
    • Korean Journal of Optics and Photonics
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    • v.22 no.4
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    • pp.191-197
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    • 2011
  • In this paper, we have designed an integrated triplexer which is the basic component for a FTTH(Fiber To The Home) system which can transmit CATV and voice/data at the same time in a single fiber. The integrated triplexer can be fabricated with a novel technique of "Micro-Block Stacking (MBS)" method which automatically aligns the optical components in the optical beam pass using accurate ceramic holders. We analyze the displacement of the optical focus according to the tolerances of the component dimensions and the assembly process using code V simulator. For the transmitter, the most serious shift of the focal points is caused by the displacements of the LD spot. So the focal point moves up to $72{\mu}m$ from the center point for ${\pm}25{\mu}m$, ${\pm}25{\mu}m$, ${\pm}30{\mu}m$ displacements. For the receiver the most serious shift of the focal points is caused by the displacements of a 0.8mm ball lens (for the analog receiving part) and a micro ball lens (for the digital receiving part), and the focal point moves up to $55{\mu}$ for ${\pm}55{\mu}m$, ${\pm}5{\mu}m$, ${\pm}55{\mu}m$ micro ball lens displacements.

Studies on Automatization of Dairy Cattle Farming 1. Development of Automatic System for Diagnosis of Pregnancy and Diseases (젖소 사양기술의 자동화를 위한 연구 1. 임신유지 여부 및 질병자동진단 시스템개발)

  • 김용준;유일정;정길도;한병성;김동원;김명순
    • Journal of Veterinary Clinics
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    • v.14 no.2
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    • pp.301-307
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    • 1997
  • These studies were performed to provide some basic informations for developing an automatic system in dairy farming cattle in order that the farmers may easily and automatically detect the maintenance of pregnancy and the fact of abortion of the pregnant cows and also to find out the diseased animals with fever. As a method of automatical detection of the maintenance of pregnancy or the fact of abortion, weighing the pregnant cows was conducted from one month-pregnancy to the term using a digital balance. From the first to the 3rd month of pregnancy the body weight of dairy cows was slowly increased (less than 2% per month), then, relatively high increase (3.4% -4.3% per month) from the fourth to the seventh month followed by decrease (3.3%) in the 8th month and very low increase (0.8-0.9%) from the 9th month to the term were shown, resulting in increase of 128.8 kg (25.05%) of body weight to be compared with the first weight. More than 107, increase of body weight to be compared with the first month-weight was denoted from the 61th month of pregnancy and more than 20% increase from the 7th or the 8th month of pregnancy as wells consequently it was presumed that detection of the maintenance of pregnancy is possible from the 4th or the 5th month of pregnancy. It was possible to diagnose a cow aborted at the 6th month by continual weighing the cow from the 1st month of pregnancy. The calved cows showed considerably higher decrease of body weight even in the third week after parturition (p<0.01)to be compared with the body weight near to the term (81.8-102.0 kg, 14-16% decrease). During the same period of 8months, the pregnant cows gained 127.4 kg (24.78% increase), whereas the non-pregnant cows gained 33.0 kg (0.71% increase) to be compared with the first weight showing considerably higher increase of body weight gain in the pregnant cows than the non-pregnant cows (p<0.01). The statistics of body temperatures of dairy cattle were collected from three clinics including the Teaching Hospital of Chonbuk University and the diseases were classified simply by the major symptoms manifested, denoting the highest temperature in respiratory disease ($39.8{\circ}C$) and the lowest in alimentary disease ($39.6{\circ} C$). These informations of body temperatures were expected to be of value for early and automatical detection of the diseased animals with fever when automatic machinery would be established. The results of periodic weighing the body weight of pregnant cows while milking were also expected to be of great use for the farmers to detect the maintenance of pregnancy and the fact of abortion when the automatic system is established in the near future.

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Gain of a New Exon by a Lineage-Specific Alu Element-Integration Event in the BCS1L Gene during Primate Evolution

  • Park, Sang-Je;Kim, Young-Hyun;Lee, Sang-Rae;Choe, Se-Hee;Kim, Myung-Jin;Kim, Sun-Uk;Kim, Ji-Su;Sim, Bo-Woong;Song, Bong-Seok;Jeong, Kang-Jin;Jin, Yeung-Bae;Lee, Youngjeon;Park, Young-Ho;Park, Young Il;Huh, Jae-Won;Chang, Kyu-Tae
    • Molecules and Cells
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    • v.38 no.11
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    • pp.950-958
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    • 2015
  • BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.

The Effects of Wearing Roller Shoes on Ground Reaction Force Characteristics During Walking (롤러 신발과 조깅 슈즈 신발 착용 후 보행 시 지면반력의 형태 비교 분석)

  • Chae, Woen-Sik
    • Korean Journal of Applied Biomechanics
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    • v.16 no.1
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    • pp.101-108
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    • 2006
  • The purpose of this study was to compare GRF characteristics during walking wearing jogging and roller shoes. Twelve male middle school students (age: $15.0{\pm}0.0\;yrs$, height: $173.6{\pm}5.0\;cm$, weight: $587.6{\pm}89.3\;N$) who have no known musculoskeletal disorders were recruited as the subjects. Kinematic data from six S-VHS camcorders(Panasonic AG456, 60 fields/s) and GRF data from two force platform; (AMII OR6-5) were collected while subjects walked wearing roller and jogging shoes in random order at a speed of 1.1 m/s. An event sync unit with a bright LED light was used to synchronize the video and GRF recordings. GRF data were filtered using a 20 Hz low pass Butterworth. digital filter and further normalized to the subject's body weight. For each trial being analyzed, five critical instants and four phases were identified from the recording. Temporal parameters, GRFs, displacement of center of pressure (DCP), and loading and decay rates were determined for each trial. For each dependent variable, paired t-test was performed to test if significant difference existed between shoe conditions (p <.05). Vertical GRFs at heel contact increased and braking forces at the end of initial double limb stance reduced significantly when going from jogging shoe to roller shoe condition. Robbins and Waked (1997) reported that balance and vertical GRF are closely related It seems that the ankle and knee joints are locked in an awkward fashion at the heel contact to compensate for the imbalance. The DCP in the antero-posterior direction for the roller shoe condition was significantly less than the corresponding value for the jogging shoe condition. Because the subjects tried to keep their upper body weight in front of the hip to prevent falling backward, the DCP for the roller shoe condition was restricted The results indicate that walking with roller shoes had little effect on temporal parameters, and loading and decay rates. It seems that there are differences in GRF characteristics between roller shoe and jogging shoe conditions. The differences in GRF pattern may be caused primarily by the altered position of ankle, knee, and center of mass throughout the walking cycle. Future studies should examine muscle activation patterns and joint kinematics during walking with roller shoes.

Reduction of Authentication Cost Based on Key Caching for Inter-MME Handover Support (MME 도메인간 핸드오버 지원을 위한 키캐싱 기반 인증비용의 감소기법)

  • Hwang, Hakseon;Jeong, Jongpil
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.5
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    • pp.209-220
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    • 2013
  • Handover is the technology to minimize data lose of mobile devices and make continuous communication possible even if the device could be moved from one digital cell site to another one. That is, it is a function that enables the mobile user to avoid the disconnection of phone conversations when moving from a specific mobile communication area to another. Today, there are a lot of ongoing researches for fast and efficient hand-over, in order to address phone call's delay and disconnection which are believed to be the mobile network's biggest problems, and these should essentially be resolved in all mobile networks. Thanks to recent technology development in mobile network, the LTE network has been commercialized today and it has finally opened a new era that makes it possible for mobile phones to process data at high speed. In LTE network environment, however, a new authentication key must be generated for the hand-over. In this case, there can be a problem that the authentication process conducted by the hand-over incurs its authentication cost and delay time. This essay suggests an efficient key caching hand-over method which simplifies the authentication process: when UE makes hand-over from oMME to nMME, the oMME keeps the authentication key for a period of time, and if it returns to the previous MME within the key's lifetime, the saved key can be re-used.

A study on characteristics related to texture, colour temperature and contrast ratio to improve the depth of stereoscopic images (깊이감 향상을 위한 질감, 색온도, 대비비 관련 특성 연구)

  • Hong, Ji-Young
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.4
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    • pp.37-42
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    • 2018
  • With advancements in digital image production technology, the branch of stereoscopic image technology has also been undergoing active development. Accordingly, research and development on cutting-edge display products for mounting stereoscopic images are currently being pursued. There are various problems that can occur when viewing 3D images. Because viewers feel visual fatigue while perceiving the depth of the images provided via an artificial method, a negative human factor such as visual fatigue has become one of the most prominent concerns, especially as it is a factor that affects the ongoing maintenance of 3D images. Therefore, by identifying the factors affecting the depth of the graphic images provided in 2D images, and subsequently using this information to develop an image processing method, we conducted depth-related experiments and analysed them under the assumption that stereoscopic images could be reproduced without visual fatigue. Thus, we analysed the most significant factors related to depth and verified the interactions by performing depth-related factors-based ANOVA variance analysis by differentially applying the texture, colour temperature, and contrast ratio to graphic images. We determined the significance of the factors related to depth and proposed a method to improve depth based on an analysis of the results of the experiments conducted in this study.

A Study on Ligiron in the Oldest Feng-Shui Book "Janggyeong" - Focus on Sasepalryongbub - (풍수고전 "장경(葬經)"에 나타난 풍수이기론에 관한 연구 - 사세팔룡법을 중심으로 -)

  • Zho, In-Choul
    • Journal of architectural history
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    • v.20 no.1
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    • pp.117-133
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    • 2011
  • This thesis is a study on the oldest feng-shui book "Janggyeong(葬經)". Specially, it is a focus on the feng-shui orientation method so called Sasepalryongbub(四勢八龍法) in that book. This method is to identify fortune and misfortune with the directions to be measured by a feng-shui compass. Sa(四) of Sase(四勢) is the four pieces of direction of dividing 12 parts for 360 degree circle. To divide for 12 parts is related with 12-Jiji(地支). Sa mean In(寅), Sin(申), Sa(巳), and Hae(亥). Se of Sase means a large stem of mountain. Generally, Sase are the big mountain stems of the four directions as In, Sin, Sa, and Hae. Pal(八) of Palryong(八龍) mean the 8 parts of equal division for 360 degree. It is related with Palgoe(八卦) that divide for 8 parts equally. Palgoe are Jin(震), Li(離), Gam(坎), Tae(兌), Geon(乾), Gon(坤), Gan(艮), and Son(巽). Ryong(龍) of Palryong(八龍) is a stem of mountain that is measured with 8 parts of equal division feng-shui compass. Sasepalryongbub make a connection between Sase and Palryong. When they are connected, it is a good fortune like as In(寅) and Li(離), Sin(申) and Gam(坎) or Gon(坤) or Gan(艮), Sa(巳) and Tae(兌) or Geon(乾), Hae(亥) and Jin(辰) or Son(巽). It is based on two theories. The one is 12-Unseongron(運星論), the other is one of Ohaengron(五行論) as Samhap-Ohaeng(三合五行). 12-Unseongron begins from dividing all human life affairs with 12 steps. Saeng(生), Wang(旺), Myo(墓) are the very important things among 12 steps. Saeng means starting, Wang means flourishing, Myo means keeping. Samhap-Ohaeng is made with Jiji of Saeng, Wang and Myo steps about 12-Unseongron. Saeng is the most important thing among of Saeng, Wang and Myo in Sasepalryongbub which means birth. Saeng of Jiji direction can help Palgoe direction as a counterpart. Like this, the good relation of Sase and Palryong is that Jiji direction can help Palgoe direction. For example, there are In(寅) of Jiji and Li(離) of Palgoe, can be explained as these kinds of the good relations. In view of Samhap-Ohaeng, In of Jiji is regared as Saeng of fire(火). This fire- Saeng-In as Jiji can help fire- Li as Palgoe. It can be said as a good condition that Sase and Palryong be connected like this case. This is the main content of Sasepalryongbub as being treated in this study.

Characterization and synthesis of aqueous pink-red ceramic ink for digital inkjet printing (잉크젯 프린팅용 pink-red 수계 무기잉크의 제조 및 특성평가)

  • Lee, Won-Jun;Hwang, Hae-Jin;Han, Kyu-Sung;Cho, Woo-Suk;Kim, Jin-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.1
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    • pp.20-26
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    • 2015
  • Ceramic ink-jet printing technology in art tiles, decorated tablewares and other porcelain products has many advantages of fast and precision printing of various images with high efficiency and low cost. For the application to ink-jet printing, ceramic ink requires a stable dispersibility with nano-sized pigments. In this paper, characteristics of pink-red aqueous ceramic ink for ink-jet printing was demonstrated. $CaCr_{0.1}Sn_{0.8}SiO_5$ pigment was synthesized using solid state reaction and deagglomerated using attrition milling. The aqueous ceramic ink contains 10 wt% of the obtained $CaCr_{0.1}Sn_{0.8}SiO_5$ nanopigment with 0.4 wt% of sodium dodecyl sulfate (SDS) as a dispersion agent. Viscosity of $CaCr_{0.1}Sn_{0.8}SiO_5$ aqueous ceramic ink was adjusted using 0.18 wt% of polyvinyl alcohol (PVA) for a suitable jetting from the nozzle. The prepared pink-red ceramic ink showed a good jetting property with formation of a single sphere-shaped droplet after $180{\mu}s$ without a tail and satellite droplet.