• Journal of the Korean Regional Science Association
• /
• v.32 no.2
• /
• pp.15-29
• /
• 2016

Suburbs rural areas in the Capital region have been diversified by the influence of the Seoul and large cities for the last 10 years. This study aims to classify suburban rural areas and to analyze the differentiated characteristics of different types in order to propose more area-specific policy approach to suburban rural areas in the Capital region. Using the cluster analysis method, the suburban rural areas in the Capital region have been classified into four types; garden villages in rural areas, urbanization areas, agricultural-industrial mixed use areas, general rural areas. The result of this study shows that the four types of suburban rural areas are much differentiated one another in terms of internal characteristics and local dynamics and problems. In order to suburban rural sustainable development in the Capital region, the policy efforts should be address the area-specific problems and policy demand for each type of suburban rural areas.

• Molecules and Cells
• /
• v.19 no.1
• /
• pp.31-38
• /
• 2005

Human embryonic stem (hES) cells have unique features including unlimited growth capacity, expression of specific markers, normal karyotypes and an ability to differentiate. Many investigators have tried to use hES cells for cell-based therapy, but there is little information about the properties of available hES cell lines. We compared the characteristics of three hES cell lines. The expression of SSEA-1, -3, -4, and APase, was examined by immunocytochemistry, and Oct-4 expression was analyzed by RT-PCR. Differentiation of the hES cells in vitro and in vivo led to the formation of embryoid bodies (EBs) or teratomas. We examined the expression of tissue-specific markers in the differentiated cells by semiquantitative RT-PCR, and the ability of each hES cell line to proliferate was measured by flow cytometry of DNA content and ELISA. The three hES cell lines were similar in morphology, marker expression, and teratoma formation. However there were significant differences (P < 0.05) between the differentiated cells formed by the different cell lines in levels of expression of tissue-specific markers such as renin, kallikrein, Glut-2, ${\beta}-$ and ${\delta}-globin$, albumin, and ${\alpha}1-antitrypsin$ (${\alpha}1-AT$). The hES cell lines also differed in proliferative activity. Our observations should be useful in basic and clinical hES cell research.

• International journal of thyroidology
• /
• v.10 no.2
• /
• pp.96-101
• /
• 2017

Background and Objectives: Anaplastic thyroid carcinoma (ATC) is commonly related with concurrent differentiated thyroid carcinoma (DTC). We aimed to examine the clinicopathologic characteristics, prognosis and gene expression of DTC with anaplastic foci. Materials and Methods: Eighteen patients with DTC with anaplastic foci were enrolled in this study. To compare the clinicopathologic characteristics and prognosis of anaplastic foci subjects with conventional ATC or DTC, we additionally included 12 ATC and 1030 DTC patients who diagnosed during same period. Immunohistochemistry was performed to check the gene expression in anaplastic foci and DTC component. Results: In anaplastic foci group, tumor size was larger ($2.5{\pm}1.3$ vs. $1.2{\pm}0.9cm$, p=0.001), distant metastasis was more frequent (11.1 vs. 0%, p=0.000) and 1-year survival rate was low (88.9 vs. 100%, p=0.000) than DTC group. In contrast, compared with ATC group, anaplastic foci group showed younger age at diagnosis ($50{\pm}16$ vs. $63{\pm}18years$, p=0.039), smaller tumor size ($2.5{\pm}1.3$ vs. $3.8{\pm}1.4cm$, p=0.027), less distant metastasis (11.1 vs. 41.7%, p=0.084) and longer 1-year survival rate (88.9 vs. 25.0%, p=0.001). Expression of p53 protein was observed in 100% of anaplastic foci, ATC and 12.5% of papillary thyroid carcinoma component. Conclusion: DTC with foci of anaplastic transformation has a worse prognosis than DTC, but a better prognosis than ATC. Our results support that DTC with anaplastic foci was intermediate state from DTC to ATC.

• Journal of Agricultural Extension & Community Development
• /
• v.16 no.1
• /
• pp.201-235
• /
• 2009

This study analyzed the demographic characteristics, strengths and weaknesses related to information acquisition of local innovation diffusion types. This study use ordered probit model to find strengths and weaknesses of innovation diffusion type in rural area. The individual characteristics of 'formal extension type', 'situational reaction diffusion type', 'agriculturist connection type', and 'systematic approach type', all differentiated according to innovation diffusion type, were analyzed. Following Choi & Choe(2008), immediacy, accessibility, referability, applicability, and satisfaction were the highest in the situational reaction diffusion type, systematic approach type, formal extension type, and farmers connection type, in the order. And there existed organic contexts among individual characteristics. So this study tried to analyze strengths and weaknesses of innovation diffusion type with a focus on immediacy, which emerged as the most important variable in the process of interpreting innovation diffusion. And the strengths and weaknesses of each innovation diffusion type were presented.

• Proceedings of the Korean Institute of Interior Design Conference
• /
• 2008.05a
• /
• pp.184-189
• /
• 2008

The change in a society have brought about other changes in the overall areas of culture and art. And have brought the advent of "object", which is a unique cultural aspect. The object became a trend of the world in a culture and art. This trend has satisfied the modern people who have look for new visual stimulation. The object appears well in the spaces restaurants and bars where are reflected in consumer's needs and change of life pattern. The reason is that the spaces restaurants and bars have expressed the differentiated and individualized image with the characteristic of the times. There are three types of the expressed characteristics of objects in interior design. These are visual, functional, and emotional characteristics. But in this study it could not be found that it characterized with one concrete vocabulary and concept. Therefore, it is need to understand the various tendency which expressed characteristics of objects in the spaces restaurants and bars.

• Journal of Korean Neurosurgical Society
• /
• v.42 no.2
• /
• pp.118-124
• /
• 2007

Objective : Adipose tissue is derived from the embryonic mesoderm and contains a heterogenous stromal cell population. Authors have tried to verify the characteristics of stem cell of adipose derived stromal cells (ADSCs) and to investigate immunohistochemical findings after transplantation of ADSC into rat brain to evaluate survival, migration and differentiation of transplanted stromal cells. Methods : First ADSCs were isolated from human adipose tissue and induced adipose, osseous and neuronal differentiation under appropriate culture condition in vitro and examined phenotypes profile of human ADSCs in undifferentiated states using flow cytometry and immunohistochemical study. Human ADSCs were transplanted into the healthy rat brain to investigate survival, migration and differentiation after 4 weeks. Results : From human adipose tissue, adipose stem cells were harvested and subcultured for several times. The cultured ADSCs were differentiated into adipocytes, osteoctye and neuron-like cell under conditioned media. Flow cytometric analysis of undifferentiated ADSCs revealed that ADSCs were positive for CD29, CD44 and negative for CD34, CD45, CD117 and HLA-DR. Transplanted human ADSCs were found mainly in cortex adjacent to injection site and migrated from injection site at a distance of at least 1 mm along the cortex and corpus callosum. A few transplanted cells have differentiated into neuron and astrocyte. Conclusion : ADSCs were differentiated into multilineage cell lines through transdifferentiation. ADSCs were survived and migrated in xenograft without immunosuppression. Based on this data, ADSCs may be potential source of stem cells for many human disease including neurologic disorder.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.17
• /
• pp.7271-7275
• /
• 2014

Background: The aim of the present study was to evaluate the presentation, clinical course and outcome between children and young adults with differentiated thyroid cancer (DTC) treated in our hospital. Materials and Methods: The medical records of 145 patients with DTC who underwent surgery followed by radioiodine and thyroid hormone (TSH) suppression were retrospectively reviewed. The follow up was between January 2006 and June 2012. These patients consisted of 38 children (age${\leq}18y$) and 107 young adult patients (age${\leq}30y$). The clinical characteristics and outcome were analyzed and compared, and the progression-free survival (PFS) was evaluated using the Kaplan-Meier method. Results: At initial diagnosis, a greater degree of extra thyroidal extension was found in children than adults patients (p<0.001). However, there was no significant difference between the two groups with regard to the tumor size and the presence of lymph node or distant metastasis (p=0.172, p=0.050 and p=0.068, respectively). The extent of surgery and the cumulative or mean dose of radioiodine were similar in both groups. During the follow up, the overall survival rate was 100% for both groups, and the PFS rate was similar in children and in young adults group (log rank test, ${\chi}^2$=0.126, p=0.723). Conclusions: In comparison to the young adult patients, DTC in children presents with more aggressive behavior, but outcomes are similar between the two groups after the intensive management of surgery followed by radioiodine and TSH suppression therapy.

• Korean Journal of Plant Resources
• /
• v.10 no.4
• /
• pp.411-417
• /
• 1997

In order to improve cultivation technique of Codonopsis lanceolata, uniconazol and paclobutrazol were treated periodically with foliar application during differentiation of node. Plant height, width and length of leaves decreased as the concentration of uniconazol and paclobutrazol increased independently of application period. Characteristics of subterranean part of all treatment by uniconazol foliar application did not show any significant difference to control, except when treated at the differentiated stage of 3rd node. But, when uniconazol was treated with the concentration of 10, 100mg $liter^1$, respectively at the differentiated stage of 12th node, fresh weight was increased to approximately 64%, 100% of control, respectively. Control and 10mg $liter^1$ treated at the differented stage of 6th node showed antitumor activity by the P388 cytotoxic screening test. Fresh weight of all treatment by paclobutrazol foliar application was not showed signigicant difference to control, except when treated at the differentiateed stage of 3rd node. But, when paclobutrazol was treated with the concentration of 10mg $liter^1$ at the differentiated stage of 12th node, fresh weight was slightly increased of control. All treatment showed slight antitumor activity by the P388 cytotoxic screening test.

• Journal of Animal Science and Technology
• /
• v.65 no.3
• /
• pp.664-678
• /
• 2023

To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37℃ and 39℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37℃ proliferated better than cultured at 39℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Hanwoo myosatellite cells cultured at 39℃ had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37℃. C2C12 cells cultured at 39℃ showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37℃. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37℃ and differentiating at 39℃ are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.25 no.3
• /
• pp.629-637
• /
• 2001

The objective of this study was to identify the cultural characteristics affecting advertising contents of the consumer products. Hofstedes(1991) five cultural characteristics such as individualism-collectivism, power distance, masculinity-feminity, avoidance of uncertainty, confucian dynamism were used. Ten kinds of consumer products were included and data were collected from France, Japan, Korea, and U. S. during 1999∼2000. All ads were evaluated and compared their cultural characteristics in terms of country, product involvement and usage, and magazine type. ANOVA, Duncan test, and Pearson correlation-coefficient were applied to analyze data. The results were shown as follows: First, most ads tended to be individual, less power oriented, feministic, and showed high tendency of uncertainty avoidance as well as long term confucianism for all the countries. And some cultural characteristics were different among countries. Japan, France, U. S. and Korea in order showed higher individualism and lower power distance. Also Korea and Japan showed relatively short term confucian culture while U. S. and France did ling term perspectives in the ads. Second, product involvement and usage type had a significant impact on the characteristics of individualism-collectivism and feminity-masculinity. That is, product ads of lower involvement and personal use were likely to show individualistic messages and to have more feministic appeals. Third, magazine type had an impact on some cultural characteristics. Womens magazines would have more individualistic, less power oriented, and feministic appeals of ads. In conclusion, global ads should be localized and differentiated in terms of some cultural characteristics and differ depending on product characteristics.