Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Comparative Characteristics of Three Human Embryonic Stem Cell Lines

  • Lee, Jung Bok (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Jin Mee (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital) ;
  • Kim, Sun Jong (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Park, Jong Hyuk (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital) ;
  • Hong, Seok Ho (Department of Obstetrics and Gynecology, Asan Medical Center) ;
  • Roh, Sung Il (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital) ;
  • Kim, Moon Kyoo (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Yoon, Hyun Soo (Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital)
  • Received : 2004.08.03
  • Accepted : 2004.09.14
  • Published : 2005.02.28
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Abstract

Human embryonic stem (hES) cells have unique features including unlimited growth capacity, expression of specific markers, normal karyotypes and an ability to differentiate. Many investigators have tried to use hES cells for cell-based therapy, but there is little information about the properties of available hES cell lines. We compared the characteristics of three hES cell lines. The expression of SSEA-1, -3, -4, and APase, was examined by immunocytochemistry, and Oct-4 expression was analyzed by RT-PCR. Differentiation of the hES cells in vitro and in vivo led to the formation of embryoid bodies (EBs) or teratomas. We examined the expression of tissue-specific markers in the differentiated cells by semiquantitative RT-PCR, and the ability of each hES cell line to proliferate was measured by flow cytometry of DNA content and ELISA. The three hES cell lines were similar in morphology, marker expression, and teratoma formation. However there were significant differences (P < 0.05) between the differentiated cells formed by the different cell lines in levels of expression of tissue-specific markers such as renin, kallikrein, Glut-2, ${\beta}-$ and ${\delta}-globin$, albumin, and ${\alpha}1-antitrypsin$ (${\alpha}1-AT$). The hES cell lines also differed in proliferative activity. Our observations should be useful in basic and clinical hES cell research.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology

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