• Title/Summary/Keyword: Different age

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Effects of Age and Gender on the Viability and Stem Cell Markers, mRNA, and Protein Expression of Bone Marrow-Derived Stem Cells Cultured in Growth Media

  • Lee, Hyunjin;Lee, Hyuna;Na, Chae-Bin;Park, Jun-Beom
    • Journal of Korean Dental Science
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    • v.11 no.2
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    • pp.62-70
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    • 2018
  • Purpose: Bone marrow has long been a source of primary cells. This study was performed to evaluate the effects of age and sex on the cellular viability and expression of stem cell markers of mRNA and on the protein expression of bone marrow stem cells (BMSCs) derived from healthy donors. Materials and Methods: Stem cells were isolated from human bone marrow and plated in culture plates. The shape of the BMSCs was observed under inverted microscope. Quantitative cellular viability was evaluated using a Cell-Counting Kit-8 assay. The expression of stem cell surface markers was tested and a series of quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot was performed to evaluate the expression in each group. Result: The shapes of the cells at 20s, 30s, and 50s were similar to each other. No significant changes in cellular viability were noted among different age groups or sex groups. The BMSCs expressed CD44, CD73, and CD90 surface markers but did not express CD14 and CD34. There were no noticeable differences in CD surface markers among the different age groups. The expressions of CD surface markers were similar between men and women. No significant differences in the secretion of vascular endothelial growth factors (VEGFs) were noted at Day 3 between different age groups. qRT-PCR regarding the expression showed differences between the age groups. However, Western blot analysis showed a decrease in expression but did not reach statistical significance (P>0.05). Conclusion: This study clearly showed no significant differences in shape, cell viability, expression of stem cell surface markers, or secretion of human VEGF among different age groups. However, western blot analysis showed a tendency of age-related decrease which did not reach statistical significance. Collectively, autologous or allogeneic BMSCs should be meticulously applied to obtain optimal results regarding age and sex.

Dietary Behaviors of Adults in Kimcheon City (경북 김천지역 성인의 식행동 특성)

  • Jin, Young-Hee
    • Journal of the Korean Society of Food Culture
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    • v.16 no.1
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    • pp.43-57
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    • 2001
  • This study was to investigate the changes in dietary practices, reasons for making dietary changes, current food consumption patterns, and eating habits. Data were collected from 515 parents whose children were attending an elementary school and a middle school in Kimcheon. Among the twenty food groups studied, subjects reported eating more fruit, vegetables, lower-fat meats, high-fat meats, reduced/skim milk, and eggs and less sugar, fats, rice, salt, potato/sweet potato, and chicken. The changes in dietary practices except high-fat meats and salt were significantly different between sex, age, education level, income level, or BMI groups. The primary reason for making dietary changes was 'concerned about family's health'. The average rate of never eating breakfast or less than 2 times per week was found to be 23.2% with a greater rate in younger age group. The rate of breakfast skipping was found to vary between different age and education groups. The regularity of mealtime and overeating practices were significantly different between age groups. Eating out practices were found to vary between different age, education, and income groups. People at younger age group in Kimcheon should be targeted for nutrition education about eating habits. These findings are applicable at the planning and implementation stages of various nutritional improvement projects.

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Analysis of Determinants of Migration by Age Groups using General Spatial Model in Korea (공간계량모형을 이용한 연령대별 인구 이동 결정 요인 분석)

  • Han, Yi-Cheol;Lee, Jeong-Jae;Jung, Nam-Su;Park, Mee-Jeong;Suh, Kyo
    • Journal of Korean Society of Rural Planning
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    • v.11 no.3 s.28
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    • pp.59-67
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    • 2005
  • According to diverse studies in population migration, there has been a strong age-dependent population distribution in Korea. It is shown that a particular age-group tends to reside in a particular locale or community and the effect possesses usually statistical significance. We quantitatively address this issue: how certain division of age group resides in different region of the country, and investigate possible cause of this migration pattern for different age groups. In this study, population migration trend at age groups of 20s, 30s, 40s and 50s has been analyzed incorporating a spatial econometrics model that accounts for diverse statistical pitfalls such as spatial autocorrelation and spatial dependency. We found that migration trend for different age group corresponds to regional characteristics differently. The study concludes with some policy implications and suggests a need of further study.

THE INITIAL MASS FUNCTION FOR THE SOLAR NEIGHBORHOOD STARS

  • Lee, See-Woo;Chun, Moo-Young
    • Journal of The Korean Astronomical Society
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    • v.19 no.2
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    • pp.51-62
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    • 1986
  • The Wielen dip over the ragne of 6 < $M_{\upsilon}$ < 9 in the luminosity function (LF) for the solar neighborhood stars could be explained by the combination of two different IMFs which yields the age of 13 billion years of the solar neighborhood. This smaller age than the Galactic age, 15 billion years indicates the slow collapse model of the Galaxy, solving the G-dwarf problem. Two different IMFs suggest two different mechanisms for star formation in the solar neighborhood.

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Degree of Satisfaction on Hospital Foods by Patients according to Age (연령에 따른 환자의 병원음식 만족도)

  • 신민자;서경화
    • Journal of the East Asian Society of Dietary Life
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    • v.13 no.6
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    • pp.542-551
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    • 2003
  • The purpose of this study was to analyze the patient's satisfaction on hospital foods according to their age. The subjects were selected among the patients treated in the Eulgi University hospital during May of 2003. Two hundred twenty one patients (110 men, 101 women) were surveyed through questionnaires in terms of satisfaction on hospital foods and the food preference. The data were statistically analysed using $\chi$$^2$-test and their correlation. Satisfaction on general taste(p<0.05) and variety of hospital foods (p<0.05) were significantly different according to age. However, saltiness and serving temperature of hospital foods were significantly different according to sex(p<0.05). The preference of cooking method for meat, fist vegetable and the preferred kinds of Kimchi(p<0.05) and milk & milk products(p<0.05) were significantly different according to age.

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Effects of Caffeine Intake on Calcium Utilization in Rats of Different Age and Sex (카페인 섭취수준이 연령이 다른 흰쥐의 체내 칼슘 이용에 미치는 영향)

  • 최미경
    • Journal of Nutrition and Health
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    • v.30 no.8
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    • pp.911-919
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    • 1997
  • The purpose of this research was to investigate the effect of caffeine levels on calcium utilization in rats of different age and sex. Calcium utilization was compared in rats of different age(4 weeks and 10 months) and sex that were fed various levels of caffeine (0, 3.5, and 7mg/100g body weight) for 3 weeks. There was no significant difference in feed intake, serum calcium level, and ash content in tibia among the groups. Fecal calcium excretion was lower in young rats than in adults, Urinary calcium excretion significantly higher in the caffeine groups than that in the no-caffeine group. Daily retention and apparent absorbability of calcium in young rats were higher than those in adult rats. However, there was no significant difference among groups of different sex and caffeine levels. The results of this study suggest that caffeine consumption promotes urinary calcium excretion.

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Adjective Ordering: Contrastive Analysis and Interlanguage

  • Jung, Woo-Hyun
    • English Language & Literature Teaching
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    • v.15 no.2
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    • pp.121-150
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    • 2009
  • This paper deals with contrastive analysis and interlanguage with respect to adjective ordering. It aimed to investigate how similar and different the orders of descriptive adjectives are in English and Korean, and how Korean EFL learners perceive the sequences of English descriptive adjectives. Data were collected from native English speakers and native Korean speakers and Korean EFL learners. The contrastive analysis showed that the order of English adjectives was size, opinion, condition, age, color, shape, material, and origin, whereas the Korean order was condition, age, opinion, color, size, shape, material, and origin. The relative order of the interlanguage was shown to be age, size, opinion, shape, condition, color, origin, and material, with the exceptions of the order of condition preceding age and that of size being the same position as condition. The interlanguage data manifested different aspects of ordering when compared with English and Korean: Some adjective combinations were similar to both English and Korean; Some were different from English or Korean; Some were different from both English and Korean. These ordering patterns are discussed in terms of such principles as the nouniness principle, the subjectivity/objectivity principle, the iconic principle, etc. On the basis of these results, some helpful suggestions are made.

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Assessing Preference for Leaf Mustard Kimchi using Conjoint Model (돌산지역 갓김치의 관광 상품에 대한 관광객의 선호도에 미치는 영향 요인 평가)

  • Jeong, Hang-Jin;Kang, Jong-Heon
    • Culinary science and hospitality research
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    • v.12 no.2 s.29
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    • pp.137-150
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    • 2006
  • The aim of this study was to determine the relative importance of a set of attributes which influence purchase decision of leaf mustard kimchi such as color, package, taste, availability and price, and to identify tourist product factor combinations which confer the highest utility to tourists of each age group. Conjoint model was used for this study. The findings from this study were as follows. First, it was found that all age groups regarded taste and price as the very important factor. Second, it was found that the products with different attribute levels were preferred by age groups. Third, it was found that the most preferred product in each age group was significantly different by cluster. Finally, it was found that the most preferred simulation product was different by age groups.

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Age Replacement Policy for A System Considering Failure Characteristics of Components (부품(部品)의 고장특성(故障特性)를 고려한 시스템의 수명교환방침(壽命交換方針))

  • Jeong, Yeong-Bae
    • Journal of Korean Society for Quality Management
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    • v.21 no.2
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    • pp.109-120
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    • 1993
  • Most systems are composed of components which have different failure chracteristics. Since the failure characteristics of components is different, it is rational and reasonable to establish a maintenance model to be considered repair and replacement policies which are proper to failure characteristics of these components. This paper proposes the age replacement time for a system composed of components which have different failure characteristics. In this model, it is assumed that a system is composed of a critical failure component, a major failure component, minor failure component. If any failure occurs to critical component before its age replacement time, the system should be replaced. If any failure does not occur until its age replacement time, preventive replacement should be performed at age replacement time T. Major component is minimal repaired if any failure occurs during operation. Minor component should be replaced as soon as failure is found. This paper determines the optimal replacement time of the system which minimize, total maintenance cost and initial stock Quantity of minor component within this optimal replacement time. Numerical example illustrates these results.

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Expression of Adipocyte-Associated Genes in the Mouse Tail Epididymal Fat at Different Postnatal Ages

  • Lee, Ki-Ho
    • Development and Reproduction
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    • v.24 no.3
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    • pp.167-176
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    • 2020
  • The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.