• Title/Summary/Keyword: Diagnostic method

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Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR

  • Kumari, Preeti;Sinha, Swati;Gahtori, Renuka;Quadiri, Afshana;Mahale, Paras;Savargaonkar, Deepali;Pande, Veena;Srivastava, Bina;Singh, Himmat;Anvikar, Anupkumar R
    • Parasites, Hosts and Diseases
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    • v.60 no.4
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    • pp.295-299
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    • 2022
  • Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic tools are required for accurate treatment. Molecular diagnosis of malaria is commonly carried out by nested polymerase chain reaction (PCR) targeting 18S rRNA gene, while this technique involves long turnaround time and multiple steps leading to false positive results. To overcome these drawbacks, we compared highly sensitive cytochrome oxidase gene-based single-step multiplex reaction with 18S rRNA nested PCR. Cytochrome oxidase (cox) genes of P. falciparum (cox-III) and P. vivax (cox-I) were compared with 18S rRNA gene nested PCR and microscopy. Cox gene multiplex PCR was found to be highly specific and sensitive, enhancing the detection limit of mixed infections. Cox gene multiplex PCR showed a sensitivity of 100% and a specificity of 97%. This approach can be used as an alternative diagnostic method as it offers higher diagnostic performance and is amenable to high throughput scaling up for a larger sample size at low cost.

A study on the quantification for Oriental Medicine Data (한의학자료의 수량화에 대한 연구)

  • Shin, Yang-Kyu
    • Journal of the Korean Data and Information Science Society
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    • v.8 no.2
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    • pp.173-181
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    • 1997
  • In oriental medicine, it is required that correct medical knowledge should be maintained for medical expert system which analyzes and diagnoses patients symptoms. Typical medical expert system has a knowledge base as its core, and the knowledge base contains a domain specific knowledge about patients records. However, oriental medicine diagnostic knowledge is formed mostly as qualitative data, knowledge could be ambiguous and uncertain. In this paper, we looked at quantification methods and propose a method for quantifying the oriental medicine diagnostic knowledge, which is improving the knowledge base of an oriental medicine expert system.

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Diagnostics for Estimated Smoothing Parameter by Generalized Maximum Likelihood Function (일반화최대우도함수에 의해 추정된 평활모수에 대한 진단)

  • Jung, Won-Tae;Lee, In-Suk;Jeong, Hae-Jeong
    • Journal of the Korean Data and Information Science Society
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    • v.7 no.2
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    • pp.257-262
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    • 1996
  • When we are estimate the smoothing parameter in spline regression model, we deal the diagnostic of influence observations as posteriori analysis. When we use Generalized Maximum Likelihood Function as the estimation method of smoothing parameter, we propose the diagnostic measure for influencial observations in the obtained estimate, and we introduce the finding method of the proper smoothing parameter estimate.

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Diagnostics of Power Cables using DC Voltage Decay method (직류전압감쇄법을 이용한 전력케이블의 절연열화진단)

  • Yi, Dong-Young;Kim, Sang-Joan;Kim, Ju-Yong;Song, Il-Keun
    • Proceedings of the KIEE Conference
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    • 1997.07e
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    • pp.1688-1691
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    • 1997
  • In this work, we have developed the off-line diagnostic system for power cable insulation using DC voltage decay method to diagnose the insulation degradation of underground power cables. Measurement errors of diagnostic system are less than 1% for $1T{\Omega}$ internal resistor. We have also developed measurement and diagnostic programs for win95 which is suitable for the database construction and the management of data. We could conclude that it is possible to apply this system to the multi-grounded underground cable systems.

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In-Process Diagnosis of Servovalve wear in Hydraulic Force Control Systems (유압실린더 힘 제어계의 인-프로세스 서보밸브 마모진단에 관한 연구)

  • Kim, S.D.;Jeon, S.H.;Chang, Y.
    • Transactions of The Korea Fluid Power Systems Society
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    • v.6 no.2
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    • pp.22-30
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    • 2009
  • An in-process method of diagnosing the spool wear of hydraulic servovalves was explored. The diagnostic method discussed in this paper is for force-control hydraulic servo systems. The key principle used is that pressure sensitivity of a servovalve drops as the valve spool wears out so that it is possible to determine the spool condition by monitoring pressure sensitivity. A diagnostic algorithm was developed and evaluated through numerical simulation and experiments. Two major steps of diagnosis are the evaluation of null bias of the servovalve and the approximation of pressure sensitivity, both of which could be successfully done during normal operation of a servo system. The difference between a new servovalve and a worn valve could be clearly detected in-process, and the diagnostic test was found to be repeatable.

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Clinical Application of Endobronchial Ultrasound Guided Transbronchial Needle Aspiration (Endobronchial Ultrasound Guided Transbronchial Needle Aspiration의 임상적 유용성)

  • HwangBo, Bin
    • Korean Journal of Bronchoesophagology
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    • v.14 no.2
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    • pp.5-9
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    • 2008
  • Endobronchial ultrasound guided transbronchial needle aspiration is a recently introduceddiagnostic method which enables real time aspiration of peritracheal and peribronchial lesions. The high sensitivity and the high diagnostic accuracy of EBUS-TBNA in the mediastinal staging of lung cancer have been reported. EBUS-TBNA also showed the high diagnostic yield in the diagnosis of lung parenchymal masses adjacent to the trachea or the large airways. EBUS-TBNA is a good diagnostic method for mediastinal diseases, such as sarcoidosis. Until now, no major complications of EBUS-TBNA have been reported. EBUS-TBNA should be considered for the mediastinal staging of lung cancer and the diagnosis of mediastinal lymphadenopathies.

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Remote Fault Diagnosis Method of Wind Power Generation Equipment Based on Internet of Things

  • Bing, Chen;Ding, Liu
    • Journal of Information Processing Systems
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    • v.18 no.6
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    • pp.822-829
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    • 2022
  • According to existing study into the remote fault diagnosis procedure, the current diagnostic approach has an imperfect decision model, which only supports communication in a close distance. An Internet of Things (IoT)-based remote fault diagnostic approach for wind power equipment is created to address this issue and expand the communication distance of fault diagnosis. Specifically, a decision model for active power coordination is built with the mechanical energy storage of power generation equipment with a remote diagnosis mode set by decision tree algorithms. These models help calculate the failure frequency of bearings in power generation equipment, summarize the characteristics of failure types and detect the operation status of wind power equipment through IoT. In addition, they can also generate the point inspection data and evaluate the equipment status. The findings demonstrate that the average communication distances of the designed remote diagnosis method and the other two remote diagnosis methods are 587.46 m, 435.61 m, and 454.32 m, respectively, indicating its application value.

Statistical Methods for Comparing Predictive Values in Medical Diagnosis

  • Chanrim Park;Seo Young Park;Hwa Jung Kim;Hee Jung Shin
    • Korean Journal of Radiology
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    • v.25 no.7
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    • pp.656-661
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    • 2024
  • Evaluating the performance of a binary diagnostic test, including artificial intelligence classification algorithms, involves measuring sensitivity, specificity, positive predictive value, and negative predictive value. Particularly when comparing the performance of two diagnostic tests applied on the same set of patients, these metrics are crucial for identifying the more accurate test. However, comparing predictive values presents statistical challenges because their denominators depend on the test outcomes, unlike the comparison of sensitivities and specificities. This paper reviews existing methods for comparing predictive values and proposes using the permutation test. The permutation test is an intuitive, non-parametric method suitable for datasets with small sample sizes. We demonstrate each method using a dataset from MRI and combined modality of mammography and ultrasound in diagnosing breast cancer.

A Study on Development of Diagnostic Index for Measure of Rural Villages Landscapes Level (농촌마을단위 경관진단지표 개발에 관한 연구)

  • Song, Hee-Jung;Kim, Dae-Sik
    • Journal of Korean Society of Rural Planning
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    • v.19 no.3
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    • pp.107-116
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    • 2013
  • In this study, it provides the diagnostic index for the rural landscape formation. For the development of diagnostic index, this study first analyzed documents and papers on the landscape formation. Landscape types are also classified by their function and then landscape index was developed by AHP method. Classification system was categorized as three steps: 2 items for 1st step, 10 items for 2nd step, and 20 items(criteria) for 3rd step. In the survey of weighting values with AHP method, the analysis result for the first step showed that rural village landscape is more important than landscape around the village by approximately 20%. In the second step, residence is rated as the most important, followed by village tree planting, and then farmland around the rural villages, greenery, and water environment. In the third step, the feng shui is rated as the most important, followed by tree planting, village forest, culture, and history. While vehicle maintenance, village alleys and pedestrian facilities are rated lower. In index of the around the village, weighting value for index of the farm land and skyline has the highest value. While species richness, water quality and water resources were rated relatively low. In the future, the rural landscapes diagnosis index will be applied to measure the level of the rural villages landscapes and it is expected to propose political support for the landscapes formation.

Painless Jaundice Caused by Clonorchis sinensis Infection: A Case Report

  • Hao, Yuhua;Bao, Wanguo;Jin, Meishan;Li, Yuxiang;Wang, Feng
    • Parasites, Hosts and Diseases
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    • v.54 no.3
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    • pp.323-327
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    • 2016
  • A man with only yellowing of the skin and eye sclera was diagnosed with clonorchiasis, which rarely manifested jaundice as the initial symptom. However, because of a lack of evidence for a diagnostic gold standard, the time until definitive diagnosis was more than a week. The diagnostic process relied on inquiring about the patient's history, including the place of residence, dietary habits, and symptoms, as well as on serological findings, an imaging examination, and pathological findings. MRCP and CT results showed mild dilatation of intrahepatic ducts and increased periductal echogenicity. The eggs were ultimately found in stool by water sedimentation method after the negative report through direct smear. DNA sequencing of PCR production of the eggs demonstrated 98-100% homology with ITS2 of Clonorchis sinensis. After anti-parasite medical treatment, the patient's symptoms were gradually relieved. Throughout the diagnostic procedure, besides routine examinations, the sedimentation method or concentration method could be used as a sensitive way for both light and heavy C. sinensis infection in the definite diagnosis.