• Journal of Embryo Transfer
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• v.18 no.1
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• pp.27-33
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• 2003

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes in vitro maturation of canine oocytes and development of canine IVM/IVF embryos. The results were summarized as follows: 1. The developmental rates to 16 cells of fresh, salts and 4$^{\circ}C$-stored oocytes with and without cumulus cells were 14.3%, 5.0% and 7.5%, 2.8% and 5.7%, 0.0%, respectively. The rate of oocytes with cumulus cells(5.7%~14.3%) was higher than that of denuded oocytes(0.0%~5.0%). 2. The developmental rate to If cells of in vitro cultured oocytes recovered from ovaries collected at different stages of the reproductive cycle were 0.0%, 10.7%, 1.5%, respectively. 3. The developmental rate to 16 cells of fresh oocytes with cumulus cell cultured for 24, 32 and 48 hrs in $CO_2$ incubator were 0.0%, 5.3%, 11.8%, respectively. The rate of oocytes cultured for 48 hrs was higher than that oocytes cultured for 24 and 32 hrs. 4. The development to If cells treated activation and non-activation oocytes were 15.0%, 6.7%, respectively. The rate of oocytes treated activation was higher than that oocyte treat non-activation.

• Journal of the Korean Child Neurology Society
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• v.26 no.4
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• pp.189-196
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• 2018

Purpose: Floppy infants or congenital hypotonia indicates decreased muscle tone in infants secondary to abnormalities of the central or the peripheral nervous system, or both. Previous literature classified its causes as those attributable to a central vs. peripheral origin; however, recent studies have introduced a newer classification describing a combined origin. We invenstigated floppy infants by applying the new etiological classification and reviewed the most common etiologies based on the age of presentation. We additionally reviewed the clinical characteristics, diagnoses, and the developmental outcomes in these infants. Methods: We retrospectively reviewed the electronic medical charts and recruited 116 infants diagnosed with floppy infant syndrome between January 2005 and December 2016 at Severance Children's Hospital. Among these infants, 66 with a confirmed diagnosis were reviewed for the etiological classification. Information regarding developmental outcomes was obtained via phone interviews with the infants' families. Results: Based on the new etiological classification, among 69 infants with a confirmed diagnosis, in 40 (34.5%) this syndrome was of central origin, in 19 (16.4%) of peripheral origin, and in 10 (8.6%) of combined origin. Prader-Willi syndrome, myotonic dystrophy, and spinal muscular atrophy were the most common disorders observed and combined hypotonia showed the poorest developmental outcome. Conclusion: The study states the importance of proper evaluation of etiological diagnosis and optimal intervention for developmental prognosis. The introduction of a new etiological group of combined hypotonia especially emphasizes regular monitoring and timely rehabilitative intervention in patients for the better quality of life in them as well as their caregivers.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.292-297
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• 2022

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

• Journal of Music and Human Behavior
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• v.20 no.1
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• pp.99-115
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• 2023

Given the ongoing discussion regarding remote music therapy following the COVID-19 pandemic and the pivotal role of parental involvement in it, this study investigated parents' perceptions on their roles in a synchronous videoconferencing music therapy for their children with developmental disabilities. A total of 32 participants participated in an online survey comprising 68 questions. Descriptive statistics summarized the collected responses, and Pearson's correlation was conducted to examine the relationship between perceived parental roles, psychological burden, and willingness to participate in future remote music therapy. The findings showed that parents of children with developmental disabilities acknowledged the benefits of tele-music therapy and possessed substantial information about its implementation. Furthermore, they reported their roles of providing physical, participatory, and mediating support. As parents exhibited greater satisfaction with their role in supporting their children's participation or managing their off-task behaviors, they perceived reduced psychological burden. These findings hold significant implications for expansion of tele-music therapy strategies to address the unique needs of children with developmental disabilities and support their parents as immediate mediators for their children.

• Journal of Korea Entertainment Industry Association
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• v.13 no.8
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• pp.619-626
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• 2019

This study was conducted to investigate the effects of a participatory rehabilitation program on sit-rise and rise-to-walk test performances, and perception and motor skills in adults with medically vulnerable individuals and, adults with developmental disabilities in particular. Seventeen adults with developmental disabilities participated in a participatory rehabilitation program using resistance bands and exercise balls, for 60 minutes once weekly over 13 weeks. Their performances were measured before and immediately after the intervention, and 12 weeks after. The findings were as follows. In the sit-rise test, the number of times rising from sitting posture increased after the intervention versus before, but the difference was not statistically significant. In the rise-to-walk test, the performance showed statistically significant difference over time, and the post-hoc test showed a significant effect after the intervention versus before. There was no significant difference in perception and motor skills. In sum, the participatory rehabilitation program positively influenced dynamic balancing related to functional activities but had no significant effect on perception and motor skills, which is related to motor control and motor learning. It is suggested that to increase the participation in community activities, reduce fall risk, and improve dynamic balancing abilities in adults with developmental disabilities, participatory rehabilitation programs should be utilized to promote the physical wellbeing.

• The Journal of Korean Academy of Sensory Integration
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• v.21 no.3
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• pp.39-53
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• 2023

Objective : The purpose was the effect of sensory integration therapy combined with an eye tracker on the sensory processing and visual perception of children with developmental disabilities. Methods : It was a single-subject study with a multiple baseline design between subjects, and the intervention applied sensory integration therapy combined with an eye tracker. Visual-motor speed and saccadic eye movements were assessed at each session of baseline and intervention periods. As pre- and post-evaluation, sensory profile, Korean-Developmental Test of Visual Perception and Trail Making Test were conducted. The results of each session evaluation and pre- and post-evaluation researched the effectiveness of the intervention through visual analysis and trend line analysis. Results : As a result of the evaluation for each session, the slope of the trend line for all children in visual-motor speed and saccadic eye movement increased sharply during the intervention compared to the baseline. As a result of the pre- and post-evaluation, the sensory processing of movement, body position, and visual changed from more than that of peers to a level similar to that of peers. In visual perception, all children's ability of Visual Closure increased. As a result of Trail Making Test conducted to confirm the improvement of children's visual tracking and visual-motor abilities, all children showed a decrease in performance time after the test compared to before. Conclusion : It was confirmed that sensory integration therapy combined with an eye tracker for developmental disabilities has effect on sensory processing and visual perception. It is expected to play an important role clinically as it can stimulate children's interest and motivation in line with recent technological improvements and the spread of smart devices.

• The Journal of Korean Academy of Sensory Integration
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• v.12 no.2
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• pp.1-12
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• 2014

Objective : The purpose of this study is to investigate about what autistic-spectrum disorder child's relationship between the sensory process types, the auditory behavioral characteristic and sociality Methods : The study performed from May to June, 2014. Targeting 15 children diagnosed as the autistic spectrum disorder and other 11 children suspected as having the autistic spectrum disorder in their ages of full 2-14 years old. This study used the Auditory Behavioral Checklist, Ewha-Check List for Autistic Children, Social Maturity Scale, School Function Assessment and sensory profile. For the data analysis, the statistical analysis was conducted with the SPSS 18.0. Results : First, in the relationship between the sensory process type and the auditory behavior, the preschool children and the school children showed some positive correlations with the hearing in the background noise and the communication, respectively. Second, the hearing in the background noise and the communication showed some meaningful relationship with sociality. Conclusion : The occupational therapists need to investigate effects of the sensory integrative intervention with sensory diet to improve sociality.

• The Journal of Korean Academy of Sensory Integration
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• v.17 no.2
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• pp.56-68
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• 2019

Objective : The purpose of this study is to provide evidence for a systematic analysis of occupational therapy interventions for the activity and participation of children and adolescents with developmental disabilities. Methods : The articles used in this study were collected from the RISS, KISS, and DBpia databases. The key words used were "children and occupational therapy" "children and sensory integration," "adolescent and occupational therapy," "adolescent and sensory integration," "developmental disorder and occupational therapy," and "developmental disorder and sensory integration." The research period was limited to January 2008 to August 2018. Seven articles in total were selected for systematic analysis. Results : Most of the included works were single-case studies, and most subjects dealt with the autism spectrum disorder. The majority of the interventions used involved sensory integration. Occupational therapy interventions were self-care (33%), major life area (33%), learning and application (11%), communication (11%), and mobility (11%). Conclusion : This study will help with understanding the current state of occupational therapy interventions for the activity and participation. On the basis of this understanding, various studies on this subject are expected to be conducted in the future.

• Journal of Convergence for Information Technology
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• v.9 no.3
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• pp.112-119
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• 2019

This study examined the role of citizen advocates in advocating for people with developmental disabilities. Citizen advocacy refers to activities that advocate and represent citizens on behalf of those who are socially vulnerable. The purpose of this study is to analyze the role concept of citizen advocates by conducting focus group interviews and dividing the 13 citizen advocates with developmental disabilities into 4 groups. It is divided into four main subjects and nine sub-themes. The main theme are 'Self-transformation of the parties', 'Restoring the rights of the parties', 'Being with the parties', and 'Expanding the world of the parties'. Sub-themes are 'Recognizing and solving the problem of self', 'Granting role to speak', 'Recovering the initiative of service use', 'Restoring the rights of existing (family and neighbor), "Preventing money harm" "Speaking instead", "Encouraging and petting people", "Promoting change in the community", and "Expanding the size of the world a little bit". Based on this, we discussed the importance of citizen advocacy as a preventive advocacy system, the relationship between citizen advocacy and professional advocacy system, the difference between volunteer and personal assistants and citizen advocacy, and considerations for citizen advocacy.

• 재활복지
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• v.22 no.2
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• pp.53-82
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• 2018

The purpose of this study was to understand the experience of persons with developmental disabilities participating in the Personalized Support Services. For case study, we conducted in-depth interviews with the persons with developmental disabilities and with the active supporters for them who were using the community welfare centers for the disabled. Through this, we have come up with three core categories: 'coming out into the community', 'experimenting with various choices in life', and 'living a subjective life'. This suggests the followings; First, it can be seen that persons with developmental disabilities who have limited communication and self-determination should be allowed to participate in the service through the participation process, and active support for the parties is needed in the process. Second, it is necessary to plan and support for them, centering on the parties, away from institution-centered services. Third, sufficient budget support should be provided for the self-determination, selection and control of the parties. Fourth, we should be able to enjoy everyday life by participating together in the local community, and change the perception of local residents to participate in the community. Fifth, continuous support is needed rather than temporary support. Finally it will be needed for us to listen to the voices of both parties and to represent their rights with all our efforts.