• Title/Summary/Keyword: Developmental growth

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Decreased Contact Inhibition in Mouse Adipose Mesenchymal Stem Cells

  • Jeon, Yunmi;Lee, Myung Sook;Cheon, Yong-Pil
    • Development and Reproduction
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    • v.16 no.4
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    • pp.329-338
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    • 2012
  • The proliferation of embryonic cells or adult stem cells in tissue is critically regulated during development and repair. How limited the proliferation of cells, so far, is not much explored. Cell-cell contact proliferation inhibition is known as a crucial mechanism regulating cell proliferation in vitro and in vivo. In this study we examined the characters of mouse subcutaneous adipose derived stem cells (msADSC) whether they lost or get contact inhibition during in vitro culture. The characters of msADSC growth after confluence were analyzed using confocal microscope and the expression profiles of contact inhibition related genes were analyzed according to the morphological changes using real-time PCR method. msADSC showed overlapping growth between them but not after passage 14. The cell shapes were also changed after passage 14. The expression profiles of genes which are involved in contact inhibition were modified in the msADSC after passage 14. The differentiation ability of msADSCs to adipocyte, chondrocyte and osteocyte was not changed by such changes of gene expression profiles. Based on these results, it is revealed that smADSC were characterized by getting of strong cell-cell contact inhibition after passage 14 but the proliferation and developmental ability were not blocked by the change of cell-cell contact proliferation inhibition. These finding will help to understand the growth of adipose tissue, although further studies are needed to evaluate the physiological meaning of the cell-cell contact proliferation inhibition during in vitro culture of msADSC.

Effects of Knockout Serum Replacement in the Culture Medium on the Proliferation of Porcine Fetal Fibroblasts In Vitro

  • Kim, Eun-Ju;Park, Jung-Joo;Choi, Young-Ju;Park, Sang Kyu;Roh, Sang-Ho
    • International Journal of Oral Biology
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    • v.35 no.1
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    • pp.1-5
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    • 2010
  • Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control), 10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells ($1.09\;{\times}\;10^5\;cells/ml$). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% ($3.0\;{\times}\;10^4\;cells/ml$) or 20% KSR ($4.8\;{\times}\;10^4\;cells/ml$) produced similar cell growth rates. In conclusion, although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.

Expression of Growth Differentiation Factor-9 in the Mouse Ovaries at Different Developmental Stages (생쥐 난소의 발생단계에 따른 Growth Differentiation Factor-9의 유전자 발현)

  • 윤세진;이경아;고정재;차광열
    • Development and Reproduction
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    • v.3 no.1
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    • pp.95-100
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    • 1999
  • Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor $\beta$ (TGF-$\beta$) superfamily. It has been known that GDF-9 is a growth factor having a crucial role in normal folliculogenesis and its expression is oocyte-specific. The present study was aimed to elucidate the expression of GDF-9 mRNA in the mouse primordial follicles as well as in the other developmental stages. The semiquantitative analysis of GDF-9 mRNA expression was conducted. Total RNA was extracted from the ICR mice ovaries at gestational day 19, postnatal day 1, day 10, day 21, and day 28, and RT-PCR was performed to measure GDF-9 and $\beta$-actin mRNA levels. Level of GDF-9 mRNA were normalized against the level of $\beta$-actin mRNA, and compared among different stages. GDF-9 mRNA was detected in all samples including the fetal ovaries that mainly consists of primordial follicles. The highest level of mRNA was observed in ovaries obtained at day 10 that mainly consists of growing follicles. The present result suggests that GDF-9 may play an important role in the early stage of folliculogenesis.

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Effect of Temperature on the Development of the Ash-gray Leaf Bug, Piesma maculata (Insecta, Hemiptera, Piesmatidae) (온도가 두줄명아주노린재의 발육에 미치는 영향 (곤충망, 매미목, 명아주노린재과))

  • Park, Pil Ryoun;Sang Ock Park
    • The Korean Journal of Ecology
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    • v.6 no.1
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    • pp.55-65
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    • 1983
  • The authors reared the ash-gray leaf bug, Piesma maculata in the growth cabinet controlled as temperature groups of $15, 20, 25, 30, 40^{\circ}C$under condition of photoperiod 16L:8D, light intensity $510{\pm}240$ lus, relative humidity $65{\pm}3%$, and analyzed the effects of temperature on the development of the insect. The results are summarized as follow: There are highly significant differences the developmental periods for the temperature groups, and between the developmental periods for the developmental stages. The egg in the temperature of 15 and $40^{\circ}C$ was hatched, but the ecdysis was impossible. The thermal threshold was $12.34^{\circ}C$and the upper lethal temperature $40.39^{\circ}C$. The total developmental periods of egg to adulate in the temperature of 20, 25, 30 and $35^{\circ}C$are 40.52, 22.37, 15.91 and 13.00 days, respectively. That is, the developmental period was decreased, as the temperature was increased. In the developmental period for the developmental stages, the developmental period of egg stage was longer than that of 25, 30 and $35^{\circ}C$, and that of 25。C was longer than that of $35^{\circ}C$. But ther was not significant differences between the developmental periods for the other temperature group. The rate of hatch at$20^{\circ}C$is the greater value as 90%, and the rates of 25, 30 and $35^{\circ}C$ are 79, 79 and 67%, respectively. That is the rate of hatch was decreased, as the temperature was increased. The mortality in the temperature of $35^{\circ}C$ is the greatest value as 68%, and those of 30, 25 and $20^{\circ}C$are 59, 59 and 41%, respectively. That is, the mortality was increased, as the temperature was increased. There was not significantly differences between the developmental period of female and male.

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Role of Golgi Apparatus on Regulation of Sec61β, COPG2 and Epidermal Growth Factor during Oocyte Maturation

  • Oh, Hae-In;Lee, Sang-Hee;Lee, Seunghyung;Lee, Seung Tae;Lee, Eunsong;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.39 no.2
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    • pp.37-41
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    • 2015
  • The oocyte undergoes various events during maturation and requires many substances for the maturation process. Various intracellular organelles are also involved in maturation of the oocyte. During the process glucose is essential for nuclear and cytoplasmic maturation, and adenosine triphosphate is needed for reorganization of the organelles and cytoskeleton. If mitochondrial function is lost, several developmental defects in meiotic chromosome segregation and maturation cause fertilization failure. The endoplasmic reticulum, a store for $Ca^{2+}$, releases $Ca^{2+}$ into the cytoplasm in response to various cellular signaling molecules. This event stimulates secretion of hormones, growth factors and antioxidants in oocyte during maturation. Also, oocyte nuclear maturation is stimulated by growth factors such as epidermal growth factor. This review summarizes roles of organelles with focus on the Golgi apparatus during maturation in oocyte.

Glioblastoma multiforme: a perspective on recent findings in human cancer and mouse models

  • Lim, Sang-Kyun;Llaguno, Sheila R. Alcantara;McKay, Renee M.;Parada, Luis F.
    • BMB Reports
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    • v.44 no.3
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    • pp.158-164
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    • 2011
  • Gliomas are the most frequently occurring primary malignancies in the central nervous system, and glioblastoma multiforme (GBM) is the most common and most aggressive of these tumors. Despite vigorous basic and clinical studies over past decades, the median survival of patients with this disease remains at about one year. Recent studies have suggested that GBMs contain a subpopulation of tumor cells that displays stem cell characteristics and could therefore be responsible for in vivo tumor growth. We will summarize the major oncogenic pathways abnormally regulated in gliomas, and review the recent findings from mouse models that our laboratory as well as others have developed for the study of GBM. The concept of cancer stem cells in GBM and their potential therapeutic importance will also be discussed.

Evolvable Neural Networks Based on Developmental Models for Mobile Robot Navigation

  • Lee, Dong-Wook;Seo, Sang-Wook;Sim, Kwee-Bo
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.7 no.3
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    • pp.176-181
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    • 2007
  • This paper presents evolvable neural networks based on a developmental model for navigation control of autonomous mobile robots in dynamic operating environments. Bio-inspired mechanisms have been applied to autonomous design of artificial neural networks for solving practical problems. The proposed neural network architecture is grown from an initial developmental model by a set of production rules of the L-system that are represented by the DNA coding. The L-system is based on parallel rewriting mechanism motivated by the growth models of plants. DNA coding gives an effective method of expressing general production rules. Experiments show that the evolvable neural network designed by the production rules of the L-system develops into a controller for mobile robot navigation to avoid collisions with the obstacles.

Identification of a likely pathogenic variant of YY1 in a patient with developmental delay

  • Bae, Soyoung;Yang, Aram;Ahn, Ja-Hye;Kim, Jinsup;Park, Hyun Kyung
    • Journal of Genetic Medicine
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    • v.18 no.1
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    • pp.60-63
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    • 2021
  • Gabriel-de Vries syndrome, caused by the mutation of YY1, is a newly defined genetic syndrome characterized by developmental delay, facial dysmorphism, and intrauterine growth retardation. A 7-month-old girl presented developmental delay and subtle facial dysmorphism including facial asymmetry, micrognathia, and low-set ears. Whole exome sequencing identified a de novo heterozygous missense variant in the YY1 (c.1220A>G; p.His407Arg) gene. Here, we examined the clinical and genetic characteristics of an infant with a novel likely pathogenic variant of YY1. This case expands the phenotypic spectrum of Gabriel-de Vries syndrome.