• Journal of Life Science
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• v.28 no.4
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• pp.421-428
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• 2018

Postprandial hyperglycemia plays an important role in the development of Type 2 Diabetes and diabetic complications. Controlling postprandial hyperglycemia is the most important factor for reducing the risks of diabetic complications in Type 2 diabetic patients. This study was designed to determine whether Portulaca oleracea L. extract suppresses the activation of carbohydrate-digesting enzymes, and lowers postprandial hyperglycemia in diabetic mice through streptozotocin. P. oleracea was extracted with either 80% ethanol (PEE) or water (PWE), and the extract solutions were concentrated. The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibition assays were performed using the chromogenic method. Normal mice and STZ-induced diabetic mice were orally treated with PEE, PWE (300 mg/kg of body weight) or acarbose (100 mg/kg of body weight), with soluble starch (2 g/kg of body weight). The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibitory effectiveness by PEE were markedly more effective than PWE, and both extracts indicated a higher effectiveness than the acarbose (positive control). The rise in postprandial blood glucose due to starch loading was markedly inhibited in the PEE group when compared to the control group in diabetic and normal mice. Furthermore, the area under the concentration-time curve values were markedly declined by the PEE injection in the diabetic group when compared to that exerted for the control group. These results demonstrate that P. oleracea extracts lower postprandial hyperglycemia by inhibiting carbohydrate-digesting enzymes, and that the ethanol extract is more efficacious than the water extract.

• Information Systems Review
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• v.11 no.2
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• pp.67-90
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• 2009

This study addresses information technology using level has an effect on the collaboration and organization's performance depending on the partnership that is important because of rapid development of information technology and internet diffusion. In the long view, information technology persistently increases the performance and expects the useful factor for the examing the organization's performance. Information technology using level leaves the lowness or highly, information technical application effort for the future value of each enterprise is necessary, the staff education which continuous for this the fact that the introduction is more important what continuously is information technology which is suitable at the enterprise of execution. The main purpose of this study is to figure out the difference between collaboration and organization's outcome using information technology using level that is moderating variable and difference the effect.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.323-334
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• 2020

Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of LtLysM1 exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in Nicotiana benthamiana together with protein immunoblotting confirmed that LtLysM1 was an N-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.5
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• pp.375-380
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• 2017

This paper reports the results of an acceleration test to predict the contact-induced failure that could occur at the cylinder-to-hole joint for the fuel rod of a sodium-cooled fast reactor (SFR). To incorporate the fuel life of the SFR currently under development at KAERI (around 35,000 h), the acceleration test method of reliability engineering was adopted in this work. A finite element method was used to evaluate the flow-induced vibration frequency and amplitude for the test parameter values. Five specimens were tested. The failure criterion during the life of the SFR fuel was applied. The S-N curve of the HT-9, the material of concern, was used to obtain the acceleration factor. As a result, a test time of 16.5 h was obtained for each specimen. It was concluded that the $B_{0.004}$ life would be guaranteed for the SFR fuel rods with 99％ confidence if no failure was observed at any of the contact surfaces of the five specimens.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.9
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• pp.809-815
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• 2017

STS 304 steel is used as pressure vessel material, and although it exhibits excellent mechanical characteristics at a low temperature, it is heavier than other materials. To address this issue, a method using cold-stretching techniques for STS 304 can be applied. In this study, a cold-stretching part and welded joint specimen were directly obtained from a cold-stretching pressure vessel manufactured according to ASME code. Fatigue crack propagation tests were carried out at room temperature and $-170^{\circ}C$ using the compliance method for stress ratios of 0.1 and 0.5. The results indicate that crack growth rate of the welded joint is higher than that of the cold-stretching part within the same stress intensity factor range. The outcome of this work is expected to serve as a basis for the development of a cold-stretched STS 304 pressure vessel.

• Journal of Internet Computing and Services
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• v.21 no.1
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• pp.17-31
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• 2020

With the rapid development of block chain technology, attempts have been made to put the block chain technology into practical use in various fields such as finance and logistics, and also in the public sector where data integrity is very important. Defense Operations In addition, strengthening security and ensuring complete integrity of the command communication network is crucial for operational operation under the network-centered operational environment (NCOE). For this purpose, it is necessary to construct a command communication network applying the block chain network. However, the block chain technology up to now can not solve the security issues such as the 51% attack. In particular, the Practical Byzantine fault tolerance (PBFT) algorithm which is now widely used in blockchain, does not have a penalty factor for nodes that behave maliciously, and there is a problem of failure to make a consensus even if malicious nodes are more than 33% of all nodes. In this paper, we propose a Adaptive Consensus Bound PBFT (ACB-PBFT) algorithm that incorporates a penalty mechanism for anomalous behavior by combining the Trust model to improve the security of the PBFT, which is the main agreement algorithm of the blockchain.

• Journal of Internet Computing and Services
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• v.21 no.1
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• pp.57-70
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• 2020

This study examines the subjective perception of software developers on happiness at work through the Q-sort methodology. Analysis of 63 respondents revealed four types happiness at work with highly differentiated characteristics: 'stability of work oriented', 'defiant self-driven, technology-oriented', 'realistic professionalism oriented', and 'genuinely technology and task-immersion oriented'. The 'defiant self-driven, technology-oriented' and 'genuinely technology and task-immersion oriented' types are interested in latest ICT and consider recognition of one's professionalism as well as self-realization result in happiness at work. On the other hand, the 'stability of work oriented' and 'realistic professionalism oriented' types did not pay much attention to one's growth of technical career but emphasized salary, welfare benefits and job promotion as the most important factors in happiness at work. Today, extraordinary SW developers are a key factor to acquire industrial competitiveness. Nations and corporations should prepare realistic ways to promote overall happiness at work by accurately understanding the varying characteristics and predisposition of domestic ICT personnel. In Korea, it is found that there is a shortage of 'genuinely technology and task-immersion oriented' SW developers. In order to secure national and industrial competitiveness in the era of the Fourth Industrial Revolution, creation of work ecosystem to promote high levels of happiness at work is required to secure quality software production and pride as an ICT professional.

• Journal of Internet Computing and Services
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• v.21 no.2
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• pp.81-90
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• 2020

The purpose of this study is to study how to apply block-chain technology to prevent data forgery and alteration in the defense sector of AI(Artificial intelligence). AI is a technology for predicting big data by clustering or classifying it by applying various machine learning methodologies, and military powers including the U.S. have reached the completion stage of technology. If data-based AI's data forgery and modulation occurs, the processing process of the data, even if it is perfect, could be the biggest enemy risk factor, and the falsification and modification of the data can be too easy in the form of hacking. Unexpected attacks could occur if data used by weaponized AI is hacked and manipulated by North Korea. Therefore, a technology that prevents data from being falsified and altered is essential for the use of AI. It is expected that data forgery prevention will solve the problem by applying block-chain, a technology that does not damage data, unless more than half of the connected computers agree, even if a single computer is hacked by a distributed storage of encrypted data as a function of seawater.

• Journal of Life Science
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• v.14 no.5
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• pp.874-881
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• 2004

Angiogenesis has been implicated in progression of inflammation, arthritis, psoriasis, atherosclerosis as well as tumor growth and metastasis. Intensive studies have been carried out to develop a strategy for cancer treatment by blocking angiogenesis. During angiogenesis, endothelial proliferation and migration essentially occurs upon activation. In this study, we compared the expression profiles of human umbilical endothelial cells activated by incubating in vitro in the rich medium containing several growth factors, and non-activated ones. cDNA targets derived from total RNAs of HUVEC activated for 13 h in M199 medium containing endothelial cell growth supplement, 20% fetal bovine serum, and heparin, after reaching 70～80% confluency, or non-activated, were hybridized onto oligonucleotide microarrays containing 1,8864 genetic elements. Unsupervised hierarchical clustering analysis resulted in two subgroups on dendrogram exhibiting activated and non-activated HUVECs. We then extracted 122 outlier genes which were shown to be up-regulated or under-expressed by at least 2-folds in activated HUVECs. Among these, 32 annotated genes were up-regulated and 38 were down-regulated in activated HUVECs. Interestingly, genes involved in cell proliferation, motility, and inflammation/ immune response were up-regulated in activated HUVEC, whereas genes for cell adhesion or vessel morphogenesis/function were down-regulated. Unexpectedly, the expression of genes well-characterized as angiogenesis markers was not changed except Eph-B4, which was down-regulated about 4 folds. 52 unknown genes were also up- or down-regulated. Therefore, these results could provide an opportunity to targeting new vascular molecules for the development of anti-angiogenic molecules.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1157-1165
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• 2007

VvMSA, a grapevine ASR which is highly inducible by sugar and abscisic acid signals was previously shown to be a transcription factor for a hexose transporter gene VvHT1. We isolated a cDNA clone, VlASR which is regulated temporally during the grape berry development by ACP RT-PCR (annealing control primer reverse transcriptase-polymerase chain reaction) and it proved identical to VvMSA. RT-PCR and real-time PCR analyses revealed that the VlASR gene was expressed in berries at fruit set and that its expression increased as berries aged but decreased at the late ripening stage. In order to understand the regulatory mechanism of the asr gene, a genomic fragment was cloned from grapevine. The genomic DNA was 1375 bp long and a sugar box (sucrose box 3 and sucrose responsive element 1) was identified in the 611 bp upstream region of the open reading frame. Analysis of the VlASR promoter::reporter gene fusion demonstrated that this promoter was expressed in transgenic Arabidopsis even without sucrose treatment. This result suggests that the ASR/VvHT1-mediated sugar/ABA signaling, previously reported in grapevine, may not function in Arabidopsis which has no ASR homologue.