• Molecules and Cells
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• v.40 no.9
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.2
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• pp.437-445
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• 2015

The layout of facilities, in relation to information and navigational displays, has great influence on subway satisfaction, and tend to vary depending on the types of subway platform and transit station. However, until now, few studies have covered such aspects as of yet. Starting from this viewpoint, the purpose of this study was to use an IPA analysis technique to analyze that satisfaction on the importance of facilities to aid in accessibility, such as elevators and escalators, transit amenities and information and navigational displays depending on the types of platform and transit station. To do so, we've classified 15 metropolitan stations according to types of platform and transit station and analyzed differences in user importance and satisfaction as well as improvements for platforms and transit stations of similar type. By the analysis results of this study, we've concluded that the proper selection and positioning of elevator and escalator facilities and information and navigational displays was important when designing the subway station according to platform type (separate platform) and or according to the transit station type (cross, L, T types) where pedestrian flow heavily intersected. We considered that such analysis results would be helpful in the design of new stations and the enhancement of existing stations pursuant to a new direction that minimizes user inconvenience, and that improvement items should be developed first according to the platform and transit station types, which would be helpful in enhancing the efficiency of the subway improvement cost.

• Animal Bioscience
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• v.36 no.2
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• pp.264-274
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• 2023

Objective: This study was conducted to evaluate the effects of β-glucan with vitamin E supplementation on the physiological response, litter performance, blood profiles, immune response, and milk composition of lactating sows. Methods: A total of 50 multiparous F₁ sows (Yorkshire×Landrace) with an average body weight (BW) of 233.6±4.30 kg and an average parity of 4.00±0.307 and their litters were used in this experiment. All sows were allotted to one of five treatments, taking into consideration BW, backfat thickness, and parity in a completely randomized design with 10 replicates. The experimental diets included a corn-soybean meal-based basal diet with or without 0.1% or 0.2% β-glucan and 110 IU vitamin E/kg diet. Results: All treatments added with β-glucan or vitamin E were statistically higher in the average daily feed intake (ADFI) of lactating sows compared to those of the control (Diet, p<0.01). Additionally, the ADFI of lactating sows was significantly higher in the groups supplemented with 0.1% β-glucan compared to 0.2% β-glucan (BG, p<0.01). There was an increasing trend in piglet weight at weaning (BG, p = 0.07), litter weight at the 21st day of lactation (BG, p = 0.07) and litter weight gain (BG, p = 0.08) in groups supplemented with 0.1% β-glucan. The addition of 110 IU vitamin E/kg diet increased vitamin E concentration significantly in lactating sows (VE, p<0.01) and exhibited a trend for higher concentrations of vitamin E (VE, p = 0.09) in piglets. Adding 0.1% β-glucan compared to 0.2% β-glucan induced a decrease in the concentration of tumor necrosis factor-α in lactating sows (BG, p = 0.06) and in piglets (BG, p = 0.09) on the 21st day of lactation. There were no significant differences in the milk composition of sows. Conclusion: Adding 0.1% β-glucan and 110 IU vitamin E/kg to a lactating sow's diet was beneficial to the growth performance of piglets by leading to an increase in the feed intake of sows and efficiently supplying vitamin E to both the sows and piglets.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.1186-1195
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• 1993

The Japanese type combine harvester has adopted rubber crawlers for the driving mechanism from first production . However, combine harvesters with movable track rollers in the rubber crawler system have been adopted recently for the purpose of stability at the time of climbing over the footpaths between rice fields, as the results of the machines becoming large. However, the dynamic characteristics of movable track rollers have not been clarified. For this reason, the design of movable track rollers depends on trial and errors. It is known that vibration characteristics of the vehicle with movable track rollers are different from the vibration characteristics of the vehicles with fixed track rollers even though the track roller arrangements are the same. Therefore, the theoretical analyses of movable track rollers must be hurried in order to formulate a reasonable track roller arrangement design. the authors have studied the vibration characteristics of the rubber crawler ve icle with fixed track rollers. in this study, the dynamic model of the vehicle with movable track roller sis compared with the dynamic model of the vehicle with fixed track rollers. Next, motions are simulated to analyze the movable track rollers by expanding the motion equation which were constructed for the dynamic model of the fixed track rollers.

• Journal of the Korean Home Economics Association
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• v.43 no.5 s.207
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• pp.43-55
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• 2005

This research was designed to investigate and compare the characteristics of preferred clothing textiles for slacks and skirt of college students. One hundred five subjects majoring in fashion design were surveyed and the subjects selected three favorite materials among 120 samples presented in a swatch book. The subject's weighted frequencies were added tv order of preference. The results of this study were as follows: 1. In the spring and fall seasons, black twill cotton drill was preferred foremost as a textile for slacks, and medium gray basket weaved cotton pinhead with stripe pattern was preferred as a textile for skirts. 2. In summer, dark bluish gray plain linen crash was the most preferred material for slacks, and pale yellow plain linen tropical cloth was preferred for skirts. 3. In winter, olive gray cotton corduroy was the most favorite textile for slacks, and moderate brown twill wool hound check was preferred for the skirt. 4. In thickness and weight, textiles for winter were thicker and heavier than those chosen for other seasons, and textiles for skirts showed various thicknesses and weights as compared with those of slacks. 5. In fiber content, natural fibers such as cotton and wool were preferred for an seasons. 6. In the weave of textiles, twiu we,3ve was preferred for spring, fall and winter season, and plain weave was preferred for summer. 7 The most preferred colors for slacks were B and PB, and those for the skirts were YR and PB. For color characteristics, solid fabrics were generally preferred, and patterned fabrics were preferred for skirts as compared with slacks.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.55 no.10
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• pp.435-442
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• 2006

One of the most important topics about the safety of electrical and electronic system is the reliability of the wiring system. The Time-Frequency Domain Reflectometry(TFDR) is a state-of-the-art system for detecting and estimating of the fault on a wiring. In this paper, We've considered the load resistance measurement on a coaxial cable using TFDR in a way of expanded application. The TFDR system was built using commercial Pci extensions for Instrumentation(PXI) and LabVIEW. The proposed real time TFDR system consisted of the reference signal design, signal generation, signal acquisition, algorithm execution and results display part. To implement real time system, all of the parts were programmed by the LabVIEW which is one of the graphical programming languages. Using the application software implemented by the LabVIEW, we were able to design a proper reference signal which is suitable for target cable and control not only the arbitrary waveform generator in the signal generation part but alto the digital storage oscilloscope in the signal acquisition part. By using the TFDR real time system with the terminal resistor on the target cable, we carried out load impedance measurement experiments. The experimental results showed that the proposed system are able not only to detect the location of impedance discontinuity on the cable but also to estimate the load resistance with high accuracy.

• International conference on construction engineering and project management
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• 2009.05a
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• pp.1300-1306
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• 2009

If a brief direction and plan for a remodeling project are decided, it may moves to a concrete design step of which to select the most suitable alternative out of applicable compounding devices to reveal dynamic drifting performance. However, the volume of knowledge baseed utility which can refer to systematic evaluation regarding remodeling element technology and the accumulation of realistic cases are not only sufficient, but also short including its system for expression and consistency. Therefore, it may necessary to deliver the main frame which make enables the Owner, designer and builder to get performance technology for applying advanced remodeling element and knowledge based utility. There is a necessity to provide an information with latest made by virtue of modeling in the 3D/4D based on construction-based knowledge etc. which we can use for life cycle of a project, as a prominent way of knowledge based utility. Then, it is sure that remodeling can become more activative by sharing of knowledge based utility formed in electronic 3D/4D which is a systematic and expressed consistently to a performance and applicability in preservation of savings. It is expected for modeling of the 3D/4D in knowledge based utility enables to verify the practicability of each technology on effective application, and the use of technology might be spread widely due its obvious and oriented expressions. Further, this knowledge based utility formed in electronic 3D/4D may applicable for VE process in addition to remodeling design fields.

• Archives of design research
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• v.15 no.1
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• pp.181-190
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• 2002

Every object's patterns is the balancing-relationship between the object's inner-energy and outer-stress. To see this as a balancing-relationship between an organism, inner-energy would be the occurrence of thermodynamic entropy which is for its existing maintenance. Outer-stress would be the friction that comes from the surrounding environment. Namely, we can see that object's figure exists in the balance between an extort to survive and fiction that disturb it. However, it is fourth that these figures are consisted of a curve and curved-surface which is not a straight line. So, it is impossible to find a figure made up of straight lines and surfaces in the existing object's world. It's true that we have regarded only a curve as the beautiful. Here, we have presented that in a curve, there exists an important function which protect our survival from the ancient humanity. And by closely observing the function in a physical dynamic-pattern sight, we've searched what is important in the design's origin that handle curve-patterns. Of course it would be more reasonable to analyze it in the standpoint of Mathematics, Chemistry, or Biology, and synthesize the points. However, due to the limitation of the researchability, we will concern about the physical structure in the design. Deficient details will be replenished through the study between educational systems. As a result, we have confirmed that, devoting the curve's physical structure-phenomenon (occurred in nature) to the design, is more beautiful in our eyes, too.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.2090-2100
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• 2011

Railway rail fastening system is the critical device which gives big influences to not only the vehicle driving stability and the orbit's structural stability against the impulsive load, but also the noise vibration and the ride comfort. As a part of the low-carbon green growth, the importance of the railroad industry is getting highlights on its excellent energy-efficiency and eco-friendliness. However, so far the Korea's domestic rail fastening system technology is not so good and the technical reliance to abroad is very heavy. In this study, we conducted comparative analysis on the rail fastening system with new and used one of the same type. And those systems are imported by Seoul Metro and are being used by it. With this basis, we developed the components and the materials and then, established the durability assessment methods appropriate to the Korean domestic circumstances. And through the reliability qualification test on the 7 parts of the rail fastening system, we've improved the reliability and guaranteed the 15 years of service lifetime. ($B_{10}Life15$) Establishment and standardization of Reliability Standard on the parts of the rail fastening system such as anti-vibration pads, guide-plate, screw spike made it possible to perform the internationally fair assessment. And it is thought that we can satisfy the manufactures' and consumers' needs of cost-cutting and qualification security by shortening of assessment period on rail fastening system.

• Journal of Korean Society of Disaster and Security
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• v.9 no.2
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• pp.9-13
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• 2016

The recent earthquakes in the peninsula've called safe areas prone to earthquakes occurred the unprecedented human and material damage. It does not have the seismic design of buildings for less than three layers, with the exception of some experts and organizations, and it does not have to read about the seriousness and evacuation tips for earthquake damage is thought to have been weighted. As mentioned above, most people are unaware of the evacuation tips or saengjonbeop, emergency supplies, such as earthquakes compared to a situation without the various needs education. So we were prepared for an earthquake is now managing investigate the cause and correct entities for the earthquake is considered as the time to prepare and formulate a policy. In this study, we investigated the cause to frequent earthquakes with respect to the Korean Peninsula and to organize for our government and local governments, NGOs, the media and individuals on how to proceed.