• Molecules and Cells
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• v.27 no.2
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.12
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• pp.1895-1898
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• 2017

This paper discusses methods for the torque ripple and noise reduction of DC motors for automotive air-conditioning based on electromagnetic field analysis. The target of the motor is a blower motor, and to reduce cogging torque and the torque ripple, the optimum model was selected by deforming the brush or commutator shape. In addition, to reduce the cogging torque, the model design was carried out by applying the skew method and the magnetization method of a magnet to the rotor. For optimization, the shape, material, and drive system of the motor were selected using an electromagnetic field as the analysis tool, and the method of reducing the cogging torque was applied to 4-pole, 12- and 13-slot motors considering the mechanical part. Lastly, this paper confirmed thatthemethod, which proposed how much noise, cogging torque, and vibration are reduced, improves through practical analysis.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.11
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• pp.199-205
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• 2000

The vibration and noise of gears is due to the vibration exciting force caused by the tooth stiffness which changes periodically as the mesh of teeth proceeds and by the transmission error, that is, the rotation delay between driving gear and driven gear caused by manufacturing error and alignment error in assembly and so on. The purpose of this study is to develop how to calculate simultaneously the optimum amounts of tooth profile modification, end relief and crowning by minimizing the vibration exciting force of helical gears. We estimate the vibration exciting force by the mesh analysis of gears. The constraints of this problem consist of contact ratio and strengths of gear teeth such as tooth fillet stress, surface durability and scoring. ADS(Automated Design Synthesis) is used as an optimization tool. And, since the aspect ratio is an important parameter of tooth modification, we investigate the relation between it and the optimum values of tooth modification. The proposed method can calculate the optimum amount of tooth modification automatically and is to be utilized to resolve the problem of vibration of helical gears.

• Earthquakes and Structures
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• v.8 no.1
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• pp.57-76
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• 2015

The Performance-based Earthquake Engineering (PBEE) concept implies the definition of multiple target performance levels of damage which are expected to be achieved (or not exceeded), when the structure is subjected to earthquake ground motion of specified intensity. These levels are associates to different return period (RP) of earthquakes and structural behaviors quantified with adopted factors or indexes of control. In this work an 8-level PBEE study is carried out, finding different curves for control index or Engineering Demand Parameters (EDP) of levels that assess the structural behavior. The results and the curves for each index of control allow to deduce the structural behavior at an a priori unspecified RP. A general methodology is proposed that takes into account a possible optimization process in the PBEE field. Finally, an application to 8-level seismic performance assessment to structure in a Spanish seismic zone permits deducing that its behavior is deficient for high seismic levels (RP > 475 years). The application of the methodology to a low-to-moderate seismic zone case proves to be a good tool of structural seismic design, applying a more sophisticated although simple PBEE formulation.

• East Asian Journal of Business Economics (EAJBE)
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• v.9 no.3
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• pp.29-43
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• 2021

Purpose - The purpose of this study is to analyze the root causes of changes in the rankings of paying taxes in Japan and Korea from 2019 to 2020, and put forward relevant policy recommendations for China from the perspective of enterprise tax burden and tax compliance costs. Research design, data, and methodology - This paper analyzes the data information of four indicators of Japan and Korea in paying taxes from 2009 to 2019, excel tool was used to summarize the essential reasons for the changes. Result - The results showed that, through the reform of tax system, especially the application of electronic tax system for tax declaration, and this is the fundamental reason why Korea has surpassed Japan in the ranking of Paying Taxes in recent five years. Conclusion - Drawing lessons from the reforms in South Korea and Japan, it is concluded that China should improve its tax ranking through two ways. First, deepen the reform of the main tax and fee system and reduce the burden of enterprise taxes and fees. Second, further simplify the tax procedures. Return rights and responsibilities to the enterprise.

• Nuclear Engineering and Technology
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• v.51 no.4
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• pp.968-976
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• 2019

This paper presents a number of verification case studies for a recently developed sensitivity/uncertainty code package. The code package, ROMUSE (Reduced Order Modeling based Uncertainty/Sensitivity Estimator) is an effort to provide an analysis tool to be used in conjunction with reactor core simulators, in particular the Virtual Environment for Reactor Applications (VERA) core simulator. ROMUSE has been written in C++ and is currently capable of performing various types of parameter perturbations and associated sensitivity analysis, uncertainty quantification, surrogate model construction and subspace analysis. The current version 2.0 has the capability to interface with the Design Analysis Kit for Optimization and Terascale Applications (DAKOTA) code, which gives ROMUSE access to the various algorithms implemented within DAKOTA, most importantly model calibration. The verification study is performed via two basic problems and two reactor physics models. The first problem is used to verify the ROMUSE single physics gradient-based range finding algorithm capability using an abstract quadratic model. The second problem is the Brusselator problem, which is a coupled problem representative of multi-physics problems. This problem is used to test the capability of constructing surrogates via ROMUSE-DAKOTA. Finally, light water reactor pin cell and sodium-cooled fast reactor fuel assembly problems are simulated via SCALE 6.1 to test ROMUSE capability for uncertainty quantification and sensitivity analysis purposes.

• Journal of the Korean Society of Systems Engineering
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• v.16 no.2
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• pp.67-77
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• 2020

Traditionally nuclear thermal hydraulic and nuclear safety has relied on numerical simulations to predict the system response of a nuclear power plant either under normal operation or accident condition. However, this approach may sometimes be rather time consuming particularly for design and optimization problems. To expedite the decision-making process data-driven models can be used to deduce the statistical relationships between inputs and outputs rather than solving physics-based models. Compared to the traditional approach, data driven models can provide a fast and cost-effective framework to predict the behavior of highly complex and non-linear systems where otherwise great computational efforts would be required. The objective of this work is to develop an AI algorithm to predict the peak fuel cladding temperature as a metric for the successful implementation of FLEX strategies under extended station black out. To achieve this, the model requires to be conditioned using pre-existing database created using the thermal-hydraulic analysis code, MARS-KS. In the development stage, the model hyper-parameters are tuned and optimized using the talos tool.

• Journal of the Korean Society for Precision Engineering
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• v.11 no.4
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• pp.58-68
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• 1994

An equilibrium approach is suggested as an effective tool for the analysis of sheet metal forming processes on the basis of force balance together with geometric relations and plasticity theroy. In computing a force balance equation, it is required to define a geometric curve approximating the shape of the sheet metal at any step of deformation from the geometric interaction between the die and the deforming sheet. Then the geometic informations for contacting and non-contacting sections of the sheet metal such as the number and length of both non-contact region, contact angle, and die radius of contact section are known from the geometric forming curve and utilized for optimization by force balance equation. In computation, the sheet material is assumed to be of normal amisotropy and rigid-phastic workhardening. It has been shown that there are good agreements between the equilibrium approach and FEM computation for the benchmark test example and auto-body panels whose sections can be assumed in plane-strain state. The proposed equilibrium approach can thus be used as a robust computational method in estimating the forming defects and forming severity rather quickly in the die design stage.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.1
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• pp.205-214
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• 2019

Android uses app cache files to improve app execution performance. However, this optimization technique may raise security issues that need to be examined. In this paper, we present a practical design of "Android app cache manipulation attack" to intentionally modify the cache files of a target app, which can be misused for stealing personal information and performing malicious activities on target apps. Even though the Android framework uses a checksum-based integrity check to protect app cache files, we found that attackers can effectively bypass such checks via the modification of checksum of the target cache files. To demonstrate the feasibility of our attack design, we implemented an attack tool, and performed experiments with real-world Android apps. The experiment results show that 25 apps (86.2%) out of 29 are vulnerable to our attacks. To mitigate app cache manipulation attacks, we suggest two possible defense mechanisms: (1) checking the integrity of app cache files; and (2) applying anti-decompilation techniques.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.702-708
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• 2020

Recently, as LED display systems have become larger, research on effective power control methods for the systems has been in progress. This paper proposes a power control method to minimize power loss due to the difference in LED characteristics for each channel of a backlight unit (BLU) system. The proposed LED driver IC has a power optimization function and detects the minimum headroom voltage for constant current operation of all channels and linearly controls the DC-DC converter output. Thus, it minimizes power consumption due to unnecessary additional voltage. In addition, it does not require a voltage sensing comparator or a voltage generation circuit for each channel. This has a great advantage in reducing the chip size and for stabilization when implementing an integrated circuit. In order to verify the proposed function, an IC was designed using Cadence and Synopsys' design tools, and it was fabricated with a Magnachip 0.35um 5V/40V CMOS process. The experiments confirmed that the proposed power control method controls the minimum required voltage of the BLU system.