• Biomedical Science Letters
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• v.24 no.2
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• pp.108-115
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• 2018

In the present study, results of the identification of Gram-positive bacilli (GPB) were analyzed by using the MALDI-TOF MS technique to score each 2-year blood culture at a university hospital. In addition, 16S rRNA sequence analyses and MALDI-TOF MS results are compared to targeting strains that had been isolated two or more times within the same patient, to evaluate the usefulness of MALDI-TOF MS in GPB identification. According to the cut-off (${\geq}1.7$) criteria, there were 410 (57.5%) reliable strains and 303 (42.5%) non-identified strains among the GPB identification results of 713 strains, using a microflex MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany). The isolation appeared most often in the following order: Corynebacterium striatum, Bacillus cereus, Bacillus subtilis, Paenibacillus urinalis, and Listeria monocytogenes. Nearly three-fourths, 66 out of 89 (74.2%) of the strains for Corynebacterium striatum; 44 out of 60 (73.3%) strains for Bacillus cereus; and all (25 out of 25, 100%) Listeria monocytogenes strains were identified by their high scores of 2.0 or higher. Most (293 strains out of 303) non-identified strains were strains isolated only once and not significant as infectious bacilli. A total of 43 out of 50 (86.0%) strains matched and were able to be identified based on the 16 rRNA sequencing comparison results of strains that were isolated twice or more within the same patient and significant as infection bacilli. Non-matching among 5 out of 7 strains was not identified, even with MALDI-TOF MS. In conclusion, GPB can be identified in blood cultures using MALDI-TOF MS. This can be done accurately with ease, rapidly, and at a low cost. It is also thought to be helpful in GPB diagnosis and treatment.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.4
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• pp.461-468
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• 2017

Autotransplantation of teeth with complete roots is being increasingly performed for orthodontic treatment or for replacement of unsalvageable teeth, but this procedure has lower survival and success rates than those obtained following transplantation of teeth with incomplete root formation. While previous autotransplantation procedures used only models of the donor tooth and recipient site, surgical guide templates created using 3D-printing technology are now available. They allow shaping of the recipient site in proper direction and to the correct depth, thereby reducing the treatment time and improving the success and survival rates. Herein, we report a case involving autotransplantation of a tooth with complete roots at the site of a congenitally missing permanent tooth using surgical guide templates generated with 3D-printing technology. The procedure resulted in favorable healing of the transplanted tooth.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.106-112
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• 2016

The purpose of this study was to examine the differences in the direct medical expenditure of community residents according to their physical activity level. The quota sampling method was used to select samples from a youth community center, senior center, community cultural center, and community recreational sports center in G city in Gyeongbuk-do. Of the 773 questionnaires distributed, 716 were completed. The questionnaires consisted of the demographic characteristics, regular exercise and physical activity levels, health status and medical costs. While the beneficial effect of regular exercise and physical activity on dental costs was not (significant), its effect on the costs of visiting hospital services was (significant). The mean differences in the total monthly direct medical expenditures on outpatient services and medicine for those with very high, high, moderate, low, and very low levels of physical activity were 7,500, 26,299, 47,517, 9,314, and 9,9978 won, respectively. The result of this study supported the findings of previous studies that regular exercise and physical activity. are not associated with the reduction of medical expenditure.

• Journal of the Korea Convergence Society
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• v.9 no.4
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• pp.85-91
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• 2018

This is a qualitative research that was carried out to analyze the experience of loneliness of the elderly people living alone using the result of focus group interviews. This study conducted interviews with 28 subjects in the Senior Welfare Center of D city. For the purpose, 28 persons were divided into 5 groups, and interviews were carried out with each group for 50-70 minutes. and the voluntary character of research participation was sufficiently explained. As a result of the research, three topic collections consisting of 'Unavoidable lonesomeness', 'Inevitable death', and 'Acceptance of my life' and seven subjects, which consist of 'Absence of spouse', 'Independence for children', 'Death of neighbor (friend)', 'Fear of death left alone', 'Laying down', 'Taking care of my health', and 'Soothing loneliness in my own way', were derived. The elderly persons living alone who participated in this research expressed their feeling of unavoidable loneliness and their effort to overcome the loneliness. Based on the findings of this research, it is hoped that diverse plans to overcome psychological loneliness of the elderly people living alone will be developed.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.36 no.5
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• pp.392-401
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• 2010

Introduction: Diabetes mellitus, as a major health problem for the elderly has been shown to alter the properties of the bone and impair bone healing around a titanium implant in both humans and animals. The aim of this study was to examine the effect of adipose-derived stem cells on the healing process around a titanium implant in streptozotocin-induced diabetic rats. Materials and Methods: Thirteen rats were divided into two groups: adipose-derived stem cells injected group and a control group. A titanium screw implant (diameter: 2.0 mm, length: 3.5 mm) was placed into both tibia of 13 rats: 13 right tibia as the control group and 13 left tibia as the experimental group. The rats were sacrificed at different intervals (1, 2, and 4 weeks) after implantation for histopathology observations and immunohistochemistric analysis. Results: The histopathological findings revealed earlier new formed bone in the experimental group than the control group. In particular, at 1 week after implantation, the experimental group showed more newly formed bone and collagen around the implant than the control group. In immunohistochemistric analysis, osteoprotegerin (OPG) expression in the experimental group increased early compared to that of the control group until 2 weeks after implantation. However, after 2 weeks, OPG expression in the experimental group was similar to OPG expression in the control group. The receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) expression in the experimental group increased early compared to that of the control group, and then decreased at 2 weeks. After 2 weeks, the level of RANKL expression was similar in both groups. Conclusion: These results suggest that adipose-derived stem cells in implantation can promote bone healing around titanium, particularly in diabetes mellitus induced animals.

• Imaging Science in Dentistry
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• v.50 no.1
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• pp.15-22
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• 2020

Purpose: This study was conducted to objectively and subjectively compare the accuracy and reliability of 2-dimensional(2D) photography and 3-dimensional(3D) soft tissue imaging. Materials and Methods: Facial images of 50 volunteers(25 males, 25 females) were captured with a Nikon D800 2D camera (Nikon Corporation, Tokyo, Japan), 3D stereophotogrammetry (SPG), and laser scanning (LS). All subjects were imaged in a relaxed, closed-mouth position with a normal smile. The 2D images were then exported to Mirror^® Software (Canfield Scientific, Inc, NJ, USA) and the 3D images into Proplan CMF^® software (version 2.1, Materialise HQ, Leuven, Belgium) for further evaluation. For an objective evaluation, 2 observers identified soft tissue landmarks and performed linear measurements on subjects' faces (direct measurements) and both linear and angular measurements on all images(indirect measurements). For a qualitative analysis, 10 dental observers and an expert in facial imaging (subjective gold standard) completed a questionnaire regarding facial characteristics. The reliability of the quantitative data was evaluated using intraclass correlation coefficients, whereas the Fleiss kappa was calculated for qualitative data. Results: Linear and angular measurements carried out on 2D and 3D images showed excellent inter-observer and intra-observer reliability. The 2D photographs displayed the highest combined total error for linear measurements. SPG performed better than LS, with borderline significance (P=0.052). The qualitative assessment showed no significant differences among the 2D and 3D imaging modalities. Conclusion: SPG was found to a reliable and accurate tool for the morphological evaluation of soft tissue in comparison to 2D imaging and laser scanning.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.2
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• pp.194-201
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• 2011

A dentigerous cyst is the most common developmental odontogenic cyst. Patients with dentigerous cyst usually feel no pain or discomfort. If it is not treated, tooth eruption might not only be impeded, but also let the tooth translocate to the unusual area. So, early detection and appropriate treatment are important. Enucleation & marsupialization are the best options to treat a dentigerous cyst. Treatment plan depends on patient's age, health, preserve & protect of important structures, and sort of cyst. In these dentigerous cysts cases, by marsupialization and using obturator, affected tooth could be achieved spontaneous eruption into the dental arch even though they were badly dislocated.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.1
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• pp.89-98
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• 2017

The aim of this study was to compared the penetration scores and microleakage levels of flowable resin in comparison to resin-based pit and fissure sealant. A total 120 extracted premolars were used and classified with group I (resin-based pit and fissure sealant), group II (flowable resin), group III (bonding agent + flowable resin), IV (fissurotomy + bonding agent + flowable resin) depending on the materials and the application methods. The penetration scores of the group treated with flowable resin following fissurotomy were similar to those of the group treated with resin-based sealant, but higher compared to those of the other groups treated with flowable resin without fissurotomy or bonding agent. The group treated with flowable resin following fissurotomy also exhibited the lowest microleakage levels. The group treated with resin-based sealant and the one treated with a bonding agent prior to the application of flowable resin showed similar microleakage levels. In conclusion, flowable resin may be applied as a pit and fissure sealant, and its application along with fissurotomy could increase the penetration scores.

• Journal of Dental Rehabilitation and Applied Science
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• v.36 no.1
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• pp.21-28
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• 2020

Purpose: After periodontal surgery, studies have found that the use of periodontal wound dressing reduces the risk of wound infection and increases healing. The purpose of this study is to evaluate the effect of attachable periodontal wound dressing on the healing and patient satisfaction after periodontal flap surgery. Materials and Methods: Twenty-eight patients requiring periodontal surgery underwent periodontal flap surgery on both quadrants of maxilla or mandible. Postoperative pain, bleeding, dietary discomfort and hypersensitivity in relation to attachable periodontal wound dressing was assessed using Visual Analogue Scale (VAS). Additional survey on frequency of burning sensation and overall satisfaction rates were assessed. Results: VAS mean values for pain, bleeding, and dietary discomfort depending on the presence and absence of attachable wound dressing were; pain: 2.82, 3.96 (P = 0.002), bleeding: 1.61, 2.54 (P = 0.008), dietary discomfort: 2.82, 4.18 (P < 0.001), respectively. Test groups with attachable wound dressing reported significantly lower rates of discomfort. No significant difference was observed in burning sensation and hypersensitivity related with wound dressing. Satisfaction was higher in 75% of patients who received wound dressing. Conclusion: According to the results of this study, patients who received attachable periodontal wound dressing reported less postoperative pain, bleeding, and dietary discomfort. There was no statistical significance related to the use of wound dressing with burning sensation and hypersensitivity.

• Applied Microscopy
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• v.30 no.3
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• pp.285-293
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• 2000

The present study has been carried out to investigate the effect of fluoride toxicity on the morphology as well as inorganic chemical constituents of rat teeth. Rats were administered sodium fluoride at dose of 0 ppm, 100 ppm, 200 ppm and 300 ppm at the beginning of pregnancy. Animals were perfused intravascularly with glutaraldehyde and the incisors were removed. Changes in the protein composition of the secretory and maturation enamel were investigated using polyacrylamide gel electrophoresis (SDS PAGE). And the enamel surface of incisors was examined under scanning electron microscope (SEM). Changes of protein quantities were found significantly in high levels fluoride administration for experimental groups compared with control. The SDS PAGE analysis demonstrated as follows In control group, secretory phase enamel protein, amelogenins, was detected more quantities than experimental group. The enamelin, presence in maturation phase enamel , showed more quantifies than control enamel with an increasing fluoride concentration in the drinking water. Also, the scanning electron micrographic data showed hypoplastic, tough, uneven, pitted and cracked enamel surfaces covered with granular deposits as a result of excessive intake of fluoride. From these results we conclude that high dose of fluoride administration leads to severe structural alterations on the enamel surface and these structural changes could be through defective mineralization.