• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.44-54
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• 2024

One of the serious modern environmental problems is pollution caused by highly toxic pesticides. Only small amounts of applied pesticides reach their target, and the rest ends up in soil and water. Chlorpyrifos is a toxic, broad-spectrum organophosphate insecticide. In humans, chlorpyrifos inhibits acetylcholinesterase (AChE) in the peripheral and central nervous system, and particularly in children, small amounts of this pesticide cause neurotoxic damage. As the toxic effects of chlorpyrifos and its persistence in the environment require its removal from contaminated sites, it is essential to study the biological diversity of chlorpyrifos-degrading microorganisms. In this study, we sought to determine the chlorpyrifos-degrading ability of the bacterial strain Ochrobactrum intermedium PDB-3. This strain was isolated from soil contaminated with various pesticides and identified as PDB-3 based on morpho-cultural characteristics, MALDI-TOF MS, and 16S rRNA. Studies were conducted for 30 days in sterile soils containing initial concentrations of 50, 75, 100, and 125 mg/kg of chlorpyrifos. To determine the degradation of chlorpyrifos, a liquid culture of the strain was added to the soil at three optical densities: 0, and after 24 and 48 h (OD = 0.03, 0.2 and 0.32). Using GX-MS, we determined that chlorpyrifos was converted to 3,5,6-trichloro-2-pyridinol (TCP). We also found that with increasing optical density, rapid degradation of the initial concentration of chlorpyrifos occurred. Sterile soil without strain PDB-3 was used as a control sample.

• International Journal of Control, Automation, and Systems
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• v.2 no.1
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• pp.83-91
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• 2004

The purpose of this paper is to obtain an accurate nonlinear system model to test various control schemes for a motion control system that requires high speed, robustness and accuracy. An industrial sewing machine equipped with a Brushless DC motor is considered. It is modeled by a neural network that is configured as an output-error dynamical system. The identified model is essentially a one step ahead prediction structure in which past inputs and outputs are used to calculate the current output. Using the model, a 2 degree-of-freedom PID controller to compensate the effects of disturbance without degrading tracking performance has been de-signed. In this experiment, it is not preferable for safety reasons to tune the controller online on the actual machinery. Experimental results confirm that the model is a good approximation of sewing machine dynamics and that the proposed control methodology is effective.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.88.1-88.1
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• 2017

We report a novel design of the "linear astigmatism-free" three mirror system (LAF-TMS). In general, the linear astigmatism is one of the most dominant aberration degrading image qualities in common off-axis systems. The proposed LAF-TMS is based on a confocal off-axis three mirror system, where higher order aberrations are minimized via our numerical optimization. The system comprises three pieces of aluminum-alloy freeform mirrors that are feasible to be fabricated with current single-point diamond turning (SPDT) machining technology. The surface figures, dimensions, and positions of mirrors are carefully optimized for a LAF performance. For higher precision-positioning mechanism, we also included alignment parts: shims (for tilting) and L-brackets (for decentering). Any possible mechanical deformation due to assembly process as well as 1-G gravity, and its influence on optical performances of the system are investigated via the finite element (FE) analysis. The LAF-TMS has low f-number and a wide field of view, which is promising for sky monitoring programs such as supernova surveys.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.04b
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• pp.273-277
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• 1995

Many hudraulic components have nonlinearities to some extent. These nonlinearities often cause the time delay, thus degrading the performance of the hydraulic control systems and making it difficult to modelthem. In this paper, a new vibration isolation control algorithm that eliminates the necessity of a sophiscated modeling of hydraulic system was proposed. The algotithm is a hybrid type control shecheme consisting of a linear controller and a hetero-synaptic neural network controller. Using this control scheme, simulations and experiments were performed for 1 DOF(Degree of freedom) and 2 DOF vibration isolation. The hybrid type control algorithm can isolate the base vibration signifcantly rather than linear control algorithm. And from the weights in hetero-synaptic neural network, we can get the 2nd equivalent differentialmodel of the hydraulic control system with on-line control operation. This equivalent model provides us with much information, such as stability and the characteristics of the control system.

• Journal of Advanced Navigation Technology
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• v.6 no.4
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• pp.306-311
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• 2002

In this paper, we have proposed the Multi-Carrier (MC) CDMA system by adopting multi-interference cancellation techniques connecting an adaptive array antenna and a CCI canceller in cascade form. And, we have analyzed the BER performance of system in AWGN channel where MUI is a major interference degrading the performance of the proposed MC CDMA system. Also, we have evaluated the degree of performance improvement in the proposed MC CDMA system. As the result of analysis, a CCI canceller is better efficient than an adaptive array antenna in the performance improvement and that the performance improvement becomes large as the number of users increases when multi-interference cancellation techniques are used in cascade form. Also, we found that performance of $10^{-5}$ in a point of view BER could be achieved when $E_b/N_o$ is more than 10 dB.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2087-2092
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• 2005

Using disturbance observer (DOB) is effective in enhancing the performance of dynamic system in the presence of disturbances. Recently the definition of disturbance has been expanded to modeling uncertainty including parameter variation, internal disturbance. Various structures of DOB have been proposed to improve sensitivity of system for better disturbance rejection performance. However in the case of improvement of sensitivity function, it tends to bring poor transient response due to cross-coupling and phase lag. Furthermore it could be very sensitive to measurement noise due to increased peak of complementary sensitivity function. In this paper, a dual disturbance observer (Dual-DOB) is proposed to reduce the effect of such cross-coupling. It is possible for us to improve the sensitivity function with additional external DOB with hardly affecting complementary sensitivity function. Thus it is able to have robustness against measurement noise. Since we are able to design DOBs of internal and external loop independently, we could prevent transient response quality from degrading while improving the sensitivity function. The proposed Dual-DOB is applied to a commercial optical disk drive tracking servo system. The experimental result shows that the Dual-DOB is an effective method in rejecting the disturbance as well as improving the tracking performance.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.6A
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• pp.893-899
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• 2000

WDM technology has been an emerging issue for the efficient use of optical links. WDM uses a number of different wavelengths that are assigned to each channel. The minimal number of optical transcievers and receivers should be used in a node to build an economic WDM transmission system without degrading system performance. Hence, the analysis of performance parameters such as throughput and delay is important to guarantee the WDM system performance. In this paper, the performance of a MAC protocol on a slotted WDM system that has a tunable transmitter(TTX), a tunable receiver(TRX), and a fixed receiver(FRX), respectively, on each node, was statistically analyzed. The computer simulation validates the performance analysis.

• Biomedical Science Letters
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• v.16 no.4
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• pp.279-285
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• 2010

Endogenous cannabinoids (endocannabinoids) display various pharmacological effects including pain control, anti-inflammation, and neuroprotection. The synthesis and release of endocannabinoids are regulated under both physiological and pathological conditions. The main degrading enzyme of endocannabinoid is fatty acid amide hydrolase (FAAH). Therefore we have developed the fluorescence-based assay system for FAAH. We established stable CosM6 cell lines expressing human FAAH. We also synthesized 2-oxo-2H-chromen-7-yl decanoate (DAEC) as a fluorogenic substrate for FAAH. When crude membrane extracts stably expressing FAAH was incubated with DAEC at $25^{\circ}C$, FAAH reacted specifically to DAEC and catalyzes the hydrolysis of DAEC into decanoic acid and highly fluorescent coumarin. Furthermore, the serin hydrolase inhibitor, phenylmethanesulfonylfluoride, inhibited the coumarin release to the reaction buffer in concentration dependent manner. This assay system is suitable for high-throughput screening since this system has simple experimental procedure and measurement method.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.9
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• pp.179-185
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• 2001

In this study, a visual sensor system for weld seam tracking the I-butt weld joints in GMA welding was constructed. The sensor system consists of a CCD camera, a diode laser with a cylindrical lens and a band-pass-filter to overcome the degrading of image due to spatters and arc light. In order to obtain the enhanced image, quantitative relationship between laser intensity and iris number was investigated. Throughout the repeated experiments, the shutter speed was set at 1-milisecond for minimizing the effect of spatters on the image, and therefore most of the spatter trace in the image have been found to be reduced. Region of interest was defined from the entire image and gray level of searched laser line was compared to that of weld line. The differences between these gray levels lead to spot the position of weld joint using central difference method. The results showed that, as long as weld line was within $^\pm$15$^\circ$from the longitudinal straight fine, the system constructed in this study could track the weld line successful1y. Since the processing time reduced to 0.05 sec, it is expected that the developed method could be adopted to high speed welding such as laser welding.

• International journal of advanced smart convergence
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• v.5 no.2
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• pp.18-23
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• 2016

Cell walls of microalgae consist of a polysaccharide and glycoprotein matrix providing the cells with a formidable defense against its environment. Anaerobic digestion (AD) of microalgae is primarily inhibited by the chemical composition of their cell walls containing biopolymers able to resist bacterial degradation. Adoption of pre-treatments such as thermal, thermal hydrolysis, ultrasound and enzymatic hydrolysis have the potential to remove these inhibitory compounds and enhance biogas yields by degrading the cell wall, and releasing the intracellular algogenic organic matter (AOM). This paper preproposal stage investigated the effect of different pre-treatments on microalgae cell wall, and their impact on the quantity of soluble biomass released in the media and thus on the digestion process yields. This Paper present optimum approach to degradation of the cell wall by ultra-sonication with practical design specification parameter for ultrasound based pretreatment system. As a result of this paper presents, a microalgae system in a wastewater treatment flowsheet for residual nutrient uptake can be justified by processing the waste biomass for energy recovery. As a conclusion on this result, Low energy harvesting technologies and pre-treatment of the algal biomass are required to improve the overall energy balance of this integrated system.