• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.677-684
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• 2002

We have investigated the expression patterns of candidates for growth stage specifically expressed genes. The expression patterns of the EPV20, aldolase A, Translationally Controlled Tumor Protein (TCTP) and Adipocyte Differentiation Related Protein (ADRP) were examined by semiquantitative RT-PCR and northern blot analysis in skeletal muscle tissues of Hanwoo, especially in the longissimus dorsi at various growth stages. The EPV20 mRNA was expressed in longissimus dorsi tissue of Hanwoo, but there was no difference of expression levels during growth stages. Though the aldolase A gene was reported to be muscle-specific and regulated at developmental stages, the expression levels of aldolase A mRNA in the longissimus dorsi tissues showed little differences at various growth stages. The expression levels of TCTP which was reported as growth-related protein regulated at translation step were gradually increased during growth of Hanwoo. The expression levels of ADRP mRNA were rapidly increased at 24-month-old longissimus dorsi tissue of Hanwoo, and decreased at 30-month-old. Our data suggest that the ADRP gene show as growth-stage dependent expression and is related to fat deposition within muscular tissue.

• Journal of Life Science
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• v.25 no.1
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• pp.1-7
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• 2015

Cyclin D is a member of the cyclin protein family, which plays a critical role as a core member of the mammalian cell cycle machinery. D-type cyclins (D1, D2, and D3) bind to and activate the cyclin-dependent kinases 4 and 6, which can then phosphorylate the retinoblastoma tumor suppressor gene products. This phosphorylation in turn leads to release or derepression of E2F transcription factors that promote progression from the G1 to S phase of the cell cycle. Among the D-type cyclins, cyclin D3 encoded by the CCND3 gene is one of the least well studied. In the present study, we have investigated the biochemistry of the proteolytic mechanism that leads to loss of cyclin D3 protein. Treatment of human prostate carcinoma PC-3-M cells with lovastatin and actinomycin D resulted in a loss of cyclin D3 protein that was completely reversible by the peptide aldehyde calpain inhibitor, LLnL. Additionally, using inhibitors for various proteolytic systems, we show that degradation of cyclin D3 protein involves the $Ca^{2+}$-activated neutral protease calpain. Moreover, the half-life of cyclin D3 protein half-life increased by at least 10-fold in PC-3M cells in response to the calpain inhibitor. We have also demonstrated that the transient expression of the calpain inhibitor calpastatin increased cyclin D3 protein in serum-starved NIH 3T3 cells. These data suggested that the function of cyclin D3 is regulated by $Ca^{2+}$-dependent protease calpain.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.114-131
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• 1992

In the present work, a two-dimensional boundary-value problem for a large amplitude motion is treated as an initial-value problem by satisfying the exact body-boundary and nonlinear free-surface boundary conditions. The present nonlinear numerical scheme is similar to that described by Vinje and Brevig(1981) who utilized the Cauchy's theorem and assumed the periodicity in the horizontal coordinate. In the present thesis, however, the periodicity in the horizontal coordinate is not assumed. Thus the present method can treat more realistic problems, which allow radiating waves to infinities. In the present method of solution, the original infinite fluid domain, is divided into two subdomains ; ie the inner and outer subdomains which are a local nonlinear subdomain and the truncated infinite linear subdomain, respectively. By imposing an appropriate matching condition, the computation is carried out only in the inner domain which includes the body. Here we adopt the nonlinear scheme of Vinje & Brevig only in the inner domain and respresent the solution in the truncated infinite subdomains by distributing the time-dependent Green function on the matching boundaries. The matching condition is that the velocity potential and stream function are required to be continuous across the matching boundary. In the computations we used, if necessary, a regriding algorithm on the free surface which could give converged stable solutions successfully even for the breaking waves. In harmonic oscillation problem, each harmonic component and time-mean force are obtained by the Fourier transform of the computed forces in the time domain. The numerical calculations are made for the following problems. $\cdot$ Forced harmonic large-amplitude oscillation(${\omega}{\neq}0,\;U=0$) $\cdot$ Translation with a uniform speed(${\omega}=0,\;U{\neq}0$) The computed results are compared with available experimental data and other analytical results.

• Molecules and Cells
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• v.27 no.3
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• pp.329-336
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• 2009

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

• Journal of Broadcast Engineering
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• v.15 no.2
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• pp.163-172
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• 2010

The free viewpoint video delivers an active contents where users can see the images rendered from the viewpoints chosen by them. Its applications are found in broad areas, especially museum tour, entertainment and so forth. As a new free-viewpoint application, this paper presents a stereoscopic free-viewpoint TIP (Tour Into Picture) where users can navigate the inside of a single image controlling a virtual camera and utilizing depth data. Unlike conventional TIP methods providing 2D image or video, our proposed method can provide users with 3D stereoscopic and free-viewpoint contents. Navigating a picture with stereoscopic viewing can deliver more realistic and immersive perception. The method uses semi-automatic processing to make foreground mask, background image, and depth map. The second step is to navigate the single picture and to obtain rendered images by perspective projection. For the free-viewpoint viewing, a virtual camera whose operations include translation, rotation, look-around, and zooming is operated. In experiments, the proposed method was tested eth 'Danopungjun' that is one of famous paintings made in Chosun Dynasty. The free-viewpoint software is developed based on MFC Visual C++ and OpenGL libraries.

• The Journal of the Acoustical Society of Korea
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• v.21 no.5
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• pp.471-477
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• 2002

Recently, lip-reading has been studied actively as an auxiliary method of automatic speech recognition(ASR) in noisy environments. However, almost of research results were obtained based on the database constructed in indoor condition. So, we dont know how developed lip-reading algorithms are robust to dynamic variation of image. Currently we have developed a lip-reading system based on image-transform based algorithm. This system recognize 22 words and this word recognizer achieves word recognition of up to 53.54%. In this paper we present how stable the lip-reading system is in environmental variance and what the main variant factors are about dropping off in word-recognition performance. For studying lip-reading robustness we consider spatial valiance (translation, rotation, scaling) and illumination variance. Two kinds of test data are used. One Is the simulated lip image database and the other is real dynamic database captured in car environment. As a result of our experiment, we show that the spatial variance is one of degradations factors of lip reading performance. But the most important factor of degradation is not the spatial variance. The illumination variances make severe reduction of recognition rates as much as 70%. In conclusion, robust lip reading algorithms against illumination variances should be developed for using lip reading as a complementary method of ASR.

• Journal of Life Science
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• v.23 no.7
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• pp.941-946
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• 2013

Local protein synthesis at subsynaptic sites plays a key role in the regulation of the protein composition in local domains. In this study, we carried out immunocytochemistry of cultured rat hippocampal neurons in various developmental stages to investigate the expression of eIF4E and its binding protein, eIF4EBP1. Both proteins were distributed in dendrites. In addition, eIF4EBP1 was highly expressed in the nucleus throughout the development, whereas eIF4E was not expressed in the nucleus. Punctate expression of eIF4E and eIF4EBP1 was evident in DIV 3. The colocalization rates of eIF4E or eIF4EBP1 puncta with PSD95 were higher in the dendrogenic than in the mature stages. In contrast, the colocalization rates of eIF4E and eIF4EBP1 puncta were higher in the mature than in the dendrogenic stages. As eIF4E is inactive when it is bound to eIF4EBP1, these data indicate that most dendritic eIF4E's are active during development but that they are mostly under inhibition in mature neurons.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.109-113
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• 2006

Subcellular localization of a protein containing nuclear localization signals (NLS) has been well studied in many organisms ranging from invertebrates to vertebrates. However, no systematic analysis of NLS-containing proteins available from Mollusks has been reported. Here, we describe in silico screening of NLS-containing proteins using the mollusks database that contains 22,138 amino acids. To screen putative proteins with NLS-motif, we used both predict NLS and perl script. As a result, we have found 266 proteins containing NLS sequences which are about 1.2% out of the entire proteins. On the basis of KOG (The eukaryotic orthologous groups) analysis, we can't predict the precise functions of the NLS-containing proteins. However, we found out that these proteins belong to several types of proteins such as chromatin structure and dynamics, translation, ribosomal structure, biogenesis, and signal transduction mechanism. In addition, we have analysed these sequences based on the classes of mollusks. We could not find many from the species that are the main subjects of phylogenetic studies. In contrast, we noticed that cephalopods has the highest number of NLS-containing proteins. Thus, we have constructed mollusks NLS database and added these information and data to the mollusks database by constructing web interface. Taken together, these information will be very useful for those who are or will be studying NLS-containing proteins from mollusks.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.26 no.3
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• pp.83-100
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• 2015

This paper aims to survey the supply status and demand of the scientific & technological information among the 1,059 small enterprises demanding the support in Busan, Ulsan and Gyeongsangnam-do which have been supported by the Branch of KISTI and to propose the approaches to improve the scientific & technological information system. As the results of this research, Busan, Ulsan and Gyeongsangnam-do have very poor business and R&D environment to the extent that 96.3% of businesses was small business (medium business only for 3.7%) and 31% of total small & medium-cized businesses has less than 10 R&D workers. Small & medium-sized businesses which have demanded the information to KISTI account for only 0.2% in average for 3 years. The information demand types are market trend (26.8%), industrial property right (22.1%), business proposal (19.9%), item exploration (16.8%) and quality certification (8.4%). The supplied informations include scientific and technological data survey, market survey, translation and information application seminar. The business achievements from the information supply include 379 cases in technological development improvement and launch of products, 88 cases in technology certifications and 414 cases in patents and utility design application and registration. By the analysis results, the small and medium-sized businesses have difficulties in using R&D information by themselves. Thus, it's proposed to establish the science and technology information center in southeastern region and develop the information advisors specialized in local specialty industry categories. It's also proposed to expand the manpower and budget of KISTI concerning the information support for small & medium-sized businesses.

• Journal of Korean Elementary Science Education
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• v.18 no.1
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• pp.19-28
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• 1999

This study aims to analyze tile research trends of science education in Korea, and suggest the direction of the desirable research, which is to help the improvement of science education practically. To achieve these aims, the papers related to the science education (paper;1288, master′s thesis;724, doctoral thesis;59), which were published from 1992 to 1996 in Korea, are classified by the criteria for the analysis and summed up. The condensed results of this study are as follows, 1. The papers published through this period, maintains the stability in aspect of quantity compared with 1980s. It is owing to the efforts of the prime investigator so that "the science education," as a part of normal science, may settle down in Korea. 2. In the case of thesis for the academic degree, the doctoral theses are rare, as the ratio of the doctoral vs. the master′s is 1:13. Even so, most of the theses were produced by a few universities(master′s thesis/Kor. Nat. Univ. of Edu.;28.3%, Ewha Woman′ Univ.;16.2%, doctoral thesis/Seoul Nat. Univ.;57.6%, Kor. Nat. Univ. of Edu.;22.0%). 3. Most of papers are preponderant at three categories among the ten categories proposed by this study (science teaching-learning material;23.5%, science teaching methods;21.4%, psychology of science education;21.1%). 4. Concerning the subject of research, these papers are not diversity and intensive, that is; the subject related to scientific concepts, scientific thinking and attitude, and analysis of scientific textbook is 17.7%, 14.3%, and 10.5% of the total, respectively. 5. Though not including this study, among the theses for the master of science education, many of theses are related to the subject of pure science. There are some suggestions related to tile results of this study. The severe preponderance ill the training process, the subject, and area of research, can be pointed out one of the flimsy in our researches of science education. Because these cannot be considered separately, the policy and support of educational administration must be needed to remove this phenomena. To improve the present situation of science education, the research funds have to be given to the practical than the theoretical subjects. Which can make promptly a settlement of tile problems and difficulties from the field of science education, and encourage the reseal,:h activities that tile front teachers take part in. Lately, the accumulation of literature and references are a prior condition in the development of academic knowledge. Therefore the data base system for the information of science education and translation of foreign literature related to science education must be achieved. In addition, the programs that tile teacher and the investigator can make good use of such an information without limited conditions, must be prepared.