• The Korea Journal of Herbology
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• v.21 no.2
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• pp.159-163
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• 2006

Objectives : The subacute toxicity was investigated in Sprague-Dawley rats orally treated with Dangguibohyel-tang (DBT). DBT has been used for anemia, in Korean medicine. Methods : Sprague-Dawley rats were administered orally with DBT at the dosages of 1,000 mg/kg for 14 days. We daily examined number of deaths, clinical signs, body weights and gross findings during the 14 days. Results : There were no clinical signs and pathological changes compared with control group. Body weights were not significantly changed between control and treatment groups. In hematological and biochemical serum parameters, all mean values appear to be within the normal range. Conclusion : These results suggest that DBT dose not induce any significant subacute oral toxicities in Sprague-Dawley rats.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.1
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• pp.31-36
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• 2006

The aim of this study was to elucidate the mechanism of anemia associated with Dangguibohyel-tang (DBT) in rats. Using cyclophosphamide-induced (30mg/kg BW) anemic rats, changes in weight gain, the levels of red blood cell (RBC), hematocrit (Hct), platelet and hemoglobin (Hgb), serum vitamin B12, ALT(GPT) levels and erythropoietin (EPO) gene expression were monitored, and compared with DBT (1,000mg/kg BW, 14d)-treated and EPO (1,000IU/kg BW, 14d, s.c.)-treated rats. Food efficiency ratio (FER) were 31.6%, in normal group, 28.1% in cyclophosphamide-induced control group, 31.7% in DBT-treated group and 25.1%, in EPO-treated group after 14 days. The levels of red blood cell (RBC), hematocrit (Hct), platelet and hemoglobin (Hgb) of DBT-treated group were significantly higher than those of control. And DBT extract administered group showed dominant effects on the recovery of Hgb level. Serum vitamin B12 and ALT(GPT) levels were significantly increased at DBT-treated groups. EPO gene expression was decreased 91,9% in control group, 79.6% in DBT-treated group and 53.9% in EPO-treated group, respectively. These results suggest that administration of DBT could prevent human patient from chemotherapy derived anemia by improving hematological value and EPO status.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.3
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• pp.572-574
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• 2006

The Dangguibohyel-tang (DBT) was used to treat anemia in traditional Korean medicine. Acute oral toxicity of DBT was studied in ICR mice. ICR mice were administered orally with dosages of 100 mg/kg (low dosage group), 200 mg/kg (middle dosage group), and 400 mg/kg (high dosage group) of DBT. We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. DBT did not show any toxic effect in ICR mice and oral LD50 value was over 400 mg/kg in ICR mice.