• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.199-206
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• 2012

Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. This study was designed to determine effect of glycerol and ethylene glycol as cryoprotectant in extender on improve the freezability of Jeju horse semen. The semen was cryopreserved with glucose-EDTA extender containing each 5% glycerol, 5% ethylene glycol, 8% glycerol or 8% ethylene glycol, respectively. Post-thawed sperm were evaluated motility, viability, Membrane integrity and acrosome integrity. Post-thawed sperm motility were not significantly differences among treatments. However, sperm viability were significantly higher (p<0.05) in 8% glycerol ($39.85%{\pm}11.41$) than in 5% glycerol treatment ($18.08%{\pm}1.61$). In membrane integrity, swelling sperm ratio was significantly higher (p<0.05) in 8% glycerol ($34.12%{\pm}11.02$) than other treatments. In the percentage of capacitated sperm assessed by CTC staining, F pattern was significantly higher in 8% ethylene glycol than 5% glycerol and 5% ethylene glycol (p<0.05). B pattern ratio was significantly increased in 5% ethylene glycol compared with 8% glcerol and 8% ethylene glycol (p<0.05). Moreover, 8% ethylene glycol treatment was significantly decreased AR pattern ratio compared with other treatments (p<0.05). It is concluded that treatment of 8% glycerol was improved the sperm viability and 8% ethylene glycol was improved the sperm ascrosome integrity after thawing. However, they were not significantly difference between 8% glycerol and 8% ethylene glycol on post-thawed sperm viability. Therefore, 8% ethylene glycol was more effective sperm cryoprotectant than 8% glycerol in Jeju Horse.

• Journal of Food Hygiene and Safety
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• v.31 no.6
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• pp.399-405
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• 2016

The objective of this study was to determine the effect of sakso 2 corn kernels and cobs extracts on antioxidant activity in rats fed a high fat-cholesterol diet (HFC) for 2 weeks. 48 male Sprague-Dawley (4-weeks-old) were randomly divided into 6 groups: normal diet (N), HFC (C), HFC and 0.05% kernel extracts of Saekso 2 (T1), HFC and 0.25% kernel extracts of Saekso 2 (T2), HFC and 0.05% cob extracts of Saekso 2 (T3), HFC and 0.25% cob extracts of Saekso 2 (T4). The weight gain in all treatment groups were significantly lower and the food efficiency ratio (FER) in all treatment groups except T3 were lower than C group. Liver index (liver weight/100 g body weight) in N group and T2 were significantly lower than C group. The level of total cholesterol in plasma of N group and T2 were significantly lower than C group and HDL-cholesterol in plasma of N group and T2 were significantly lower than C group. Malondialdehyde (MDA) concentration of thiobarbituric acid reactive substances in N group, T3 and T4 were significantly lower than C group. Activity of catalase (CAT) in all treatment groups were lower than C group. These result suggest that saekso 2 corn kernels and cobs extracts may reduce oxidative damage through the activation of antioxidative defense systems in rats fed high fat-cholesterol diets.

• Journal of Nutrition and Health
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• v.41 no.5
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• pp.391-401
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• 2008

Probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including IBD. However, few probiotics have undergone appropriate preclinical screening in vivo. Kefir is considered a probiotic, benefiting the host through its effects in the intestinal tract. Despite numerous studies examining the action of probiotics on the host organism, few have analyzed the effects on intestinal environment. We assessed the protective effect of kefir for three weeks before inducing colitis with 2% dextran sodium sulfate for five days. The DSS loads were similar in all DSS treatment group. The results of the experiment are as follows. Food intake and FER of experimental groups were not significantly different each other, but water consumption tended to be higher in all DSS treatment groups as compared with the normal control. And visual inspection of feces revealed mild diarrhea in rat given 2% DSS. The anti-inflammatory activity of kefir was determined by myeloperoxidase activity during the DSS treatment, and there was no significant difference in any group. The levels of thiobarbituric acid reactive substances (TBARS) as a colonic lipid peroxidation were significantly lower in the kefir intake groups than in rats treated with 2% DSS alone. The DNA % in tail and tail moment values as a DNA damage level of the blood lymphocytes in kefir intake groups tended to be lower than 2% DSS treatment alone, especially tail lengths were significantly diminished. According to the colonic histopathological assay, there were a severe inflammation of lamina propria and submucosa and mild edema in mucosa and sub mucosa in DSS alone treated group. We found a slight regenerative change in kefir treatment groups. In our experiments, this means that ulcerative colitis related to oxidative injury might be prevented by kefir as a probiotic. Further studies of the potential benefits of kefir as a probiotic in inflammatory condition are encouraged.

• Asian Journal of Turfgrass Science
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• v.15 no.2
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• pp.51-64
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• 2001

Summer stress is one of the serious problems on cool-season grass at the soccer fields in Korea during heavy rainy season. This study was conducted to intestigate the effect of shade net with regard to its percent (0, 50, 75), color (black, green), height (0 cm, 30 cm) and time (7 hr, 24 hr) on turf canopy temperature, light intensity, leaf color, turf performance, clipping yield and root dry weight of cool-season turfgrass. Turf canopy temperature was 6~13$^{\circ}C$ under black and green shade net when temperature was over 4$0^{\circ}C$. Light intensity was also decreased from 40 to 94% under black and green shade net compared to control. Black shade net was more effective than green net in reduction of temperature and light intensity. Green shade net was found to be better for photosynthesis of cool-season grass. Leaf color, turf performance, clipping yield, and root dry weight were better and increased under 50% and 75% shade net. 50% black shade net with 30cm height and 7 hr treatment showed the best turf performance. It can be concluded that 50% and 75% green shade net can be used fur reducing summer stress on cool-season grass after soccer marches during heavy rain season. The shade net decreased the turf canopy temperature and reduced heating damage of cool-season turfgrass.

• Korean Journal of Agricultural Science
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• v.3 no.2
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• pp.145-151
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• 1976

This study was conducted to determine the effect of slaked lime and straw used on the soil pH in the flooded condition and yield of rice grown in AKIOCHI paddy field and their residual effects on the rice plants. The results obtained were summarized as follow: when lime and straw were applied, there was on the average 41% of yield increase over plots treated with three elements of chemical fertilizers. When lime plus straw were used, the growth rate at later stage of rice plant was prominent. Damage due to helminthosporium and blast were found less, the rate of lower-leaf death was low, and grain number, per head, filled grain ratio, and weight of rice grain were higher than control. When lime plus straw were used, higher amount of silicate, calcium, nitrogen and potassium was found in the plants at heading stage. The residual effects of lime plus straw were 20% in the first year, about 10% in the second year and 5% in the third year, respectively. Soil pH was affected by both straw and slaked lime, and it was fixed about 8 days after applying in the flooded condition. The following formulae was suggested from the results in the flooded conditions. $$pH=5.5293+8.6007X_1+2.7836X_2-{6.7422X_1}^2-{1.8522X_2}^2-7.000X_1X_2$$ ($X_1$=slaked lime, $X_2$=straw)

• Korean Chemical Engineering Research
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• v.54 no.6
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• pp.863-870
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• 2016

We investigated the physical/chemical phenomena that a slow loss of $CO_2$ inventory into sodium after the sodium-$CO_2$ boundary failure in printed circuit heat exchangers (PCHEs), which is considered for the supercritical $CO_2$ Brayton cycle power conversion system of a sodium-cooled fast reactor (SFR). The first phenomenon is plugging inside narrow sodium channels by micro cracks and the other one is damage propagation referred to as wastage combined with the corrosion/erosion effect. Experimental results of plugging shows that sodium flow immediately stopped as $CO_2$ was injected through the nozzle at $300{\sim}400^{\circ}C$ in 3 mmID sodium channels, whereas sodium flow stopped about 60 min after $CO_2$ injection in 5 mmID sodium channels. These results imply that if pressure boundary of sodium-$CO_2$ fails a narrow sodium channel would be plugged by reaction products in a short time whereas a relatively wider sodium channel would be plugged with higher concentration of reaction products. Wastage by the erosion effect of $CO_2$ (200~250 bar) hardly occurred regardless of the kinds of materials (stainless steel 316, Inconel 600, and 9Cr-1Mo steel), temperature ($400{\sim}500^{\circ}C$), or the diameter of the $CO_2$ nozzle (0.2~0.8 mm). Velocities at the $CO_2$ nozzle were specified as Mach 0.4~0.7. Our experimental results are expected to be used for determining the design parameters of PCHEs for their safeties.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.28 no.2
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• pp.435-459
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• 1998

In order to achieve a successful endodontic treatment, root canals must be obturated three-dimensionally without causing any damage to apical tissues. Accurate length determination of the root canal is critical in this case. For this reason, I've used the conventional periapical radiography, Digora/sup (R)/(digital imaging system) and Root ZX/sup (R)/(the frequency dependent type apex locator) to measure the length of the canal and compare it with the true length obtained by cutting the tooth in half and measuring the length between the occlusal surface and the apical foramen. From the information obtained by these measurements, I was able to evaluate the accuracy and clinical usefulness of each systems. whether the thickness of files used in endodontic therapy has any effect on the measuring systems was also evaluated in an effort to simplify the treatment planning phase of endodontic treatment. 29 canals of 29 sound premolars were measured with #15, #20, #25 files by 3 different dentists each using the periapical radiography. Digora/sup (R)/ and Root ZX/sup (R)/. The measurements were then compared with the true length. The results were as follows: 1. In comparing mean discrepancies between measurements obtained by using periapical radiography(mean error: -0.449±0.444 mm), Digora/sup (R)/(mean error: -0.417±0.415 mm) and Root ZX/sup (R)/(mean error: 0.123±0.458 mm) with true length. periapical radiography and Digora/sup (R)/ system had statistically significant differences(p<0.05) in most cases while Root ZX/sup (R)/ showed none(p>0.05). 2. By subtracting values obtained by using periapical radiography, Digora/sup (R)/ and Root ZX/sup (R)/ from the true length and making a distribution table of their absolute values. the following analysis was possible. In the case of periapical film. 140 out of 261<53.6％) were clinically acceptable satisfying the margin of error of less than 0.5 mm. 151 out of 261 (53,6％) were acceptable in the Digora/sup (R)/ system while Root ZX/sup (R)/ had 197 out of 261(75.5％) within the limits of 0.5mm margin of error. 3. In determining whether the thickness of files has any effect on measuring methoths, no statistically significant differences were found(p>0.05). 4. In comparing data obtained from these methods in order to evaluate the difference among measuring methods, there was no statistically significant difference between periapical radiography and Digora/sup (R)/ system(p>0.05), but there was statistically significant difference between Root ZX/sup (R)/ and periapical radiography(p<0.05). Also there was statistically significant difference between Root ZX/sup (R)/ and Digora/sup (R)/ system(p<0.05). In conclusion, Root ZX/sup (R)/ was more accurate when compared with the Digora/sup (R)/ system and periapical radiography and seems to be more effective clinically in determining root canal length. But Root ZX/sup (R)/ has its limits in determining root morphology and number of roots and its accuracy becomes questionable when apical foramen is open due to unknown reasons. Therefore the combined use of Root ZX/sup (R)/ and the periapical radiography are mandatory. Digora/sup (R)/ system seems to be more effective when periapical radiographs are needed in a short period of time because of its short processing time and less exposure.

• Tuberculosis and Respiratory Diseases
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• v.42 no.2
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• pp.184-205
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• 1995

Background: Pulmonary toxicity by bleomycin has multiple mechanisms including direct tissue toxicity due to oxygen-derived free radicals and indirect toxicity through amplification of pulmonary inflammation. To evaluate the effect of chelators or free radical scavenger to lung damage induced by bleomycin, penicillamine as a copper chelator, deferoxamine as an iron chelator and vitamin E as a free radical scavenger were administered. Methods: Two hundred Wistar rats were divided into five groups: Control, bleomycin treated, bleomycin-penicillamine treated, bleomycin-deferoxamine treated, and bleomycin-vitamin E treated groups. Rats sacrificed on day 1, day 3, day 4, day 7, day 14, and day 28 after treatment. Bronchoalveolar lavage, light microscopic and immunohistologic studies for type I, III, IV collagens, fibronectin, laminin and NBD phallicidin were evaluated. Results: There was a significant increase in the total cell counts of bronchoalveolar lavage on day 1 from all treated animals and vitamin treated group showed an abrupt decrease in total cell counts with decrease of neutrophils on day 3. Bleomycin-vitamin E treated group had the least histologic changes such as pulmonary fibrosis. The alveolar basement membranes were positive for type IV collegen and laminin. Basement membranes of bleomycin, bleomycin-penicillamine, or bleomycin-deferoxamine treated groups were disrupted and fragmented on day 4 or 7. The bleomycin-vitamin E treated group had intact basement membranes until day 28. Conclusion: Bleomycin-induced pulmonary fibrosis was related to the severity of acute injury to oxygen radicals or activation of neutrophils and disruption of basement membrane. Vitamin E seemed to be the most effective antioxidant in the inhibition of bleomycin-induced pulmonary injury and fibrosis.

• Spatial Information Research
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• v.18 no.4
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• pp.43-53
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• 2010

A dam is effective in stable supply of water required in daily life and reduction of damage from floods. hut a lot of land or houses arc submerged due to the construction of a darn heavily affecting environment in surrounding areas. In order to improve and support daily life environment. surrounding a dam, many projects have been conducted. and the study has focused on analyzing bow to calculate error characteristics of scale effect for submerged area by using GIS spatial overlay. First, as a result of areal error in submerged area by scale based on a 1/3,000 digital topographic map, it was found that the 1/5,000 digital topographic map is 9.5 times, 9.0 times and 10.5 times more accurate than the 1/25,000 digital topographic map, respectively, in the total of areal error, standard error and areal error for submerged area. Second, as a result of analysis on areal error in areas surrounding a dam, it has been found that Jinan-eup in Jinan-gun registered the largest difference in area within 2km and 2~5km catchment area by recording 13.8 times and 20.6 times, respectively, in the 1/5,000 digital topographic map compared to the 1/25,000 digital topographic map. In addition, in areas out of catchment area within 2km, the area of occupation was very small, so there were no characteristics in error. The out of catchment area, Nami-myeon in Geumsan-gun recorded the largest errors of 31.8 times. Finally, it was found that the ratio of the total areal error in area surrounding a dam, standard error and the total areal error in the entire area using 1,5000 digital topographic map is 7.4 times, 11.8 times and 7.4 times more accurate than the 1/25,000 digital topographic map.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.11-20
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• 2014

In this work, the antioxidative effects and active component analysis of Gnaphalium affine D. DON. (G. affine) extracts were investigated. All experiments were performed with 70% ethanol extract, ethyl acetate fraction and aglycone fraction of the G. affine extract. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$) of ethyl acetate fraction ($6.15{\mu}g/mL$) of the G. affine was higher than that of (+)-${\alpha}$-tocopherol ($8.89{\mu}g/mL$), which is known as a reference control. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 70% ethanol extract ($1.60{\mu}g/mL$), ethyl acetate fraction ($0.075{\mu}g/mL$) and aglycone fraction ($2.28{\mu}g/mL$) of extract of G. affine on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system using the luminol-dependent chemiluminescence assay were much higher than that of L-ascorbic acid ($6.88{\mu}g/mL$). The cellular protective effects of 70% ethanol extract (${\tau}_{50}$ = 52.0 min) and aglycone fraction of the extract (${\tau}_{50}$ = 60.6 min) on the $^1O_2$-induced cellular damage of human erythrocytes were exhibited the higher protective effect than (+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 38.0 min), known as a lipophilic antioxidant. TLC and HPLC were used to analyse active components in the aglycone fraction of the extract. Results showed that luteolin and apigenin were main components. These results suggest that the G. affine extract can be applied to an effective antioxidant in scavenging ROS including radicals.