Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Effect of Saekso 2 Corn Kernels and Cobs Extracts on Antioxidant Activity in Rats Fed High Fat-cholesterol Diet

옥수수 색소 2호 품종의 알곡과 속대 추출물을 첨가한 고지방-고콜레스테롤 식이가 흰쥐의 항산화 활성에 미치는 영향

  • Lee, Ki Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Jai Eun (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Hong, Soo Young (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Tae hee (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Noh, Hee Sun (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Si Chang (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Park, Jong yeol (Hongcheon Maize Experiment, Gangwon-do Agicultural Research and Experiment Services) ;
  • Ahn, Mun Seob (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Hee Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agicultural Research and Experiment Services)
  • 이기연 (강원도농업기술원 농식품연구소) ;
  • 김재은 (강원도농업기술원 농식품연구소) ;
  • 홍수영 (강원도농업기술원 농식품연구소) ;
  • 김태희 (강원도농업기술원 농식품연구소) ;
  • 노희선 (강원도농업기술원 농식품연구소) ;
  • 김시창 (강원도농업기술원 농식품연구소) ;
  • 박종열 (강원도농업기술원 옥수수연구소) ;
  • 안문섭 (강원도농업기술원 농식품연구소) ;
  • 김희연 (강원도농업기술원 농식품연구소)
  • Received : 2016.10.04
  • Accepted : 2016.10.10
  • Published : 2016.12.30
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Abstract

The objective of this study was to determine the effect of sakso 2 corn kernels and cobs extracts on antioxidant activity in rats fed a high fat-cholesterol diet (HFC) for 2 weeks. 48 male Sprague-Dawley (4-weeks-old) were randomly divided into 6 groups: normal diet (N), HFC (C), HFC and 0.05% kernel extracts of Saekso 2 (T1), HFC and 0.25% kernel extracts of Saekso 2 (T2), HFC and 0.05% cob extracts of Saekso 2 (T3), HFC and 0.25% cob extracts of Saekso 2 (T4). The weight gain in all treatment groups were significantly lower and the food efficiency ratio (FER) in all treatment groups except T3 were lower than C group. Liver index (liver weight/100 g body weight) in N group and T2 were significantly lower than C group. The level of total cholesterol in plasma of N group and T2 were significantly lower than C group and HDL-cholesterol in plasma of N group and T2 were significantly lower than C group. Malondialdehyde (MDA) concentration of thiobarbituric acid reactive substances in N group, T3 and T4 were significantly lower than C group. Activity of catalase (CAT) in all treatment groups were lower than C group. These result suggest that saekso 2 corn kernels and cobs extracts may reduce oxidative damage through the activation of antioxidative defense systems in rats fed high fat-cholesterol diets.

고지방-고콜레스테롤 식이와 색소 2호 알곡 및 속대 추출물의 첨가량을 달리한 식이를 2주간 급여한 결과, 색소 2호 알곡 및 속대 추출물 첨가로 인한 식이섭취량 감소효과는 없었으며 체중 증가율은 대조군(C)대비 모든 처리군에서 유의적으로 감소하였고, 식이효율에서는 처리군(T3)을 제외하고 모든 처리군에서 유의적으로 감소하였다. 장기중량은 실험군 간의 체중 당 신장 무게는 유의적인 차이는 없었으며 체중 당 간 무게는 대조군(C)이 정상군(N)에 비하여 유의적으로 증가하였고, 처리군(T2)가 대조군(C)대비 유의적으로 낮았다. 혈청 중 중성지방 함량은 전체 실험군에서 유의적 차이는 없었으며, 총콜레스테롤 함량은 처리군(T2)이 대조군(C)대비 유의적으로 낮았고, HDL-콜레스테롤 함량은 처리군(T2)이 대조군(C)대비 유의적으로 높았다. 고지방-고콜레스테롤 식이로 인하여 대조군(C)의 간 조직 내 MDA 함량이 정상군(N)에 비하여 유의적으로 증가하였으며 처리군 T3와 T4가 대조군(C) 대비 간 조직 내 MDA 함량이 유의적으로 감소하는 경향을 보였다. 고지방-고콜레스테롤 식이로 인하여 대조군(C)의 catalase 활성은 정상군(N)에 비하여 유의적으로 감소하였으며 모든 색소 2호 알곡 및 속대 추출물 처리군(T1~T4)의 catalase 활성은 정상군(N)과 비슷한 수준으로 유의하게 증가하였다.

Keywords

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