• 한국식품영양과학회지
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• 제40권10호
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• pp.1447-1452
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• 2011

우리나라 버섯 생산량의 약 80%를 차지하는 느타리버섯(Pleurotus ostreatus)의 유통 중에 발생하는 갈변을 방지하기 위한 기초 조사로 갈변원인 효소인 polyphenol oxidase(PPO)를 분리 정제하여 그 특성을 조사하였다. 느타리버섯으로부터 3개의 PPO isoforms(PO-I, PO-II-1, PO-II-2)을 분리하였으며, gel-filteration을 이용한 분자량 확인 결과 PO-II-1은 void volume(70 kDa), PO-I과 PO-II-2는 include volume(6 kDa)에서 분리되는 것으로 보아 PO-II-1은 70 kDa 이상, PO-I과 PO-II-2는 6 kDa 이하인 것으로 확인되었다. 분리된 3개의 PPO isoform들을 partially denatured-PAGE 후, activity staining을 이용하여 분석한 결과, 비록 이들 PPO isoforms PO-II-1과 PO-II-2에 다수의 단백질들이 들어있기는 하지만 PPO 활성을 보이는 band가 하나로 나타남으로써 분리된 PPO isoform들이 실제 PPO 활성을 갖고 있는 단백질로 확인되었으며 다른 PPO isoform이 혼합되지 않은 단일 PPO isoform으로 분리되었음을 알 수 있다. Hydrophobic interaction chromatography에서 분리된 isoform PO를 이용하여 특성을 조사한 결과 최적 반응온도와 pH는 일반적인 다른 과채류와는 달리 $50{\sim}55^{\circ}C$, pH 5.5에서 높은 활성을 나타내었으며, 열 안정성 실험에 있어서는 $60^{\circ}C$에서 45분간 가열 처리 시 약 40%의 PPO activity가 남아있는 반면, $80^{\circ}C$에서 30분간 가열 처리 시 PPO가 완전히 불 활성화되었다. 그리고 chlorogenic acid와 pyrogallol에 대하여 높은 기질 특이성을 보였다.

• Radiation Oncology Journal
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• 제8권1호
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• pp.1-6
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• 1990

SCK 종양세포를 이식받은 mice에 Fluosol-DA $20\%$를 정맥주사한 후 이 종양세포를 추출하여 in vitro 실험으로 측정해 본 방사선 체포생존곡선의 Do와 Dq의 값은 Fluosol-DA $20\%$를 투여하지 않은 대조군과 대동소이하여 Fluosol-DA $20\%$가 방사선 감수성 자체에 미치는 영향은 미미하다고 해석되었다. 또한 Fluosol-DA $20\%$만을 투여한 종양의 산소분압($PO_2$)에는 별 변화가 없었으나 Fluosol-DA $20\%$를 Carbogen 흡입하에 투여시킨 종양에선 대조군의 산소분압 중앙값 4 mmHg가 62mmHg로 10배이상 증가되어 reoxygenation effect를 직접 증명할 수 있었고 hypoxic cell fraction도 약 8배정도 감소됨을 규명하였다. 한편 Carbogen만을 단독으로 흡입시킨 종양의 경우에도 산소분압의 증가를 관찰할 수 있었으나 Fluosol-DA $20\%$ 병용군보다는 산소분압상승 효과가 미약함을 관찰할 수 있었다. 따라서 Fluosol-DA $20\%$에 의한 방사선 반응의 향상은 방사선 감수성의 증가보다는 reoxygenation의 결과이며 reoxygenation 효과를 개선하기 위해서는 Fluosol-DA의 단독투여보다는 Car-bogen 흡입하에서 Fluosol-OA $20\%$의 투여가 이상적이라는 결론을 얻었다.

• 건축역사연구
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• 제22권4호
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• pp.7-18
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• 2013

Integrated-Salmi can be suggested as a character of Da-po type Kong-po in Korea's traditional architecture. However, only few studies are made on this subject yet. This study is to investigate the integration process of Salmi through cases of certain Kong-po which have the same number of inner and outer Chul-mok and are also known to precede in time. The results of the study are like below. First, Integrated-Salmi is more dominant in inner and outer 3 Chul-mok Kong-po than in inner and outer 2 Chul-mok Kong-po. Second, While inner and outer 2 Chul-mok prevail in Yeong-nam region, inner and outer 3 Chul-mok prevail in Ho-nam region. Third, integrated Salmi of Inner and outer 3 Chul-mok, first appeared in internal Jusangpo to be carried over to internal Juganpo then eventually to outer Salmi. Fourth, the reason why integrated form precede in internal salmi of inner and outer 3 Chul-mok Kong-po is due to 2 main factors. First of which is the impact of integrated Boaji that developed in Da-po type since the early 16th century. The second factor is downward expansion of Cho-gak's Boaji which was adopted to cope with disorders made in structure of Chum-Cha, due to inner and outer 3 Chul-mok type buddhist temples' wide reconstruction throughout Jeolla region following the Japanese invasion of Korea in 1592. This study is to disclose the regularity of development process of Salmi's Cho-gak(草刻) which is a characteristic of Korea's Da-po type Kong-po.

• 한국해양학회지:바다
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• 제19권2호
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• pp.125-130
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• 2014

해저 하구의 자하수 중 콜로이드 상 $^{210}Pb$ (반감기: 22.2년)과 $^{210}Po$ (반감기: 138일)의 분포 특성을 알아내기 위해서, 용존 상(<10 kDa)과 콜로이드 상($10kDa-0.45{\mu}m$)의 $^{210}Pb$과 $^{210}Po$ 시료를 채취하여 측정하였다. 함평만(2010년 7월)과 제주도(2011년 1월)의 연안 지하수 중 용존 상 $^{210}Pb$의 농도는 $0.21-2.52mBqL^{-1}$ 범위를 보였고, 콜로이드 상에서는 $0.10-1.71mBqL^{-1}$ 범위를 보였다. 용존 상 $^{210}Po$의 농도는 $0.12-2.07mBqL^{-1}$ 범위를 보였고, 콜로이드 상에서는 $0.03-0.97mBqL^{-1}$ 범위를 보였다. 총 용존 상에서 콜로이드 상 $^{210}Pb$과 $^{210}Po$의 비율은 각각 $40{\pm}5%$와 $28{\pm}5%$를 차지하였다. 이 결과는 해저를 통한 미량 원소의 해양 유출에 콜로이드가 중요한 역할을 한다는 것을 나타낸다.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.450-456
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• 1999

A high molecular-weight water-soluble fraction(PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-IIIa-1 and PO-IIIa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G-75 and Sepharose CL-6B gel permeation chromatographies. The PO-IIIa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% $ITCH_{50}$ (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-IIIa-2 were composed of TGalp1 ->, ->6Galp1->, ->2,6Galp1->, and ->Manp1->. The PO-IIIa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-IIIa-2, was further purified into two fractions, unbound proteoglycan (PO-IIIa-1A) and bound glucomannan (PO-IIIa-lB), by affinity chromatography using ConA-Sepharose CL-4B. The anti-complementary activity of each affinity purified fraction decreased as compared to that of the native PO-IIIa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anti-complementary activity.

• Biomolecules & Therapeutics
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• 제4권2호
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• pp.174-183
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• 1996

The general pharmacological properties of Artemisia extract powder (DA-9601) produced from Artemisia asiatica leaves were investigated in mice, rats, guinea pigs and rabbits. DA-9601 at the dose of 800 mg/kg po had no influences on general behaviour, barbital sleeping time and motor coordination of mice. The material at the oral dose of 800 mg/kg did exhibit neither analgesic action nor hypothermic effect. Anticonvulsant action, muscle relaxant action and the effect on intestinal propulsion were not identified at 800 mg/kg po. In the isolated ileum and trachea of guinea pig, the material did not show direct erect and inhibitory action of chemically or electrically stimulated contraction at the concentration of $2\times10^{-5}$g/ml. The sinus rates of atria and contractility of papillary muscle of guinea pig were not influenced by DA-9601 at a dose of $2\times10^{-5}$g/ml. No influences on blood pressure and respiration were observed at 40 mg/kg iv, in rabbits. However, transient decreases in blood pressure of rabbits were observed as given 120 mg/kg in iv route with slight respiratory depression, and slight diuretic effect could be found without any changes in $Na^+$ and $K^+$ excretion.

• 건축역사연구
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• 제21권5호
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• pp.69-82
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• 2012

This study is basically about four-guardian-statutes-building-gate in 17th Century. In the field of art-history, there are four-guardian-statutes made of clay in order that the statutes are so gigantic and grotesque enough to threaten all the devils. This purpose of this study is to make sure that the similar variation occurred at the four-guardian-statutes-building-gate in 17th century. The results of this study are as follows. First, only Da-Po style four-guardian-statutes-building-gates were built in famous four temples separately from 1612 until the Manchu war of 1636. And there are gigantic four-guardian-statutes made of clay in the building. Second, there are Chul-mok Ik-gong style buildings were built in 1660s at Bo-Rim-Sa and Neung-Ga-Sa. The buildings including four-guardian-statutes-building-gate of Song-gwang-sa built in 1636 probably are all similar to earlier Da-Po style four-guardian-statutes-building-gates in the viewpoint of structural type and size of building. Third, it began to build Ik-gong style four-guardian-statutes-building-gates in 1676 at Su-ta-sa.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2015년도 자성 및 자성재료 국제학술대회
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• pp.99-99
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• 2015

• Bulletin of the Korean Chemical Society
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• 제28권8호
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• pp.1317-1321
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• 2007

Arginine conjugated PAMAM dendrimer, PAMAM-R was modified with propylene oxide via hydroxylation of primary amines of arginine residues. About 49 amines were detected to be converted to amino alcohols by 1H NMR. The newly synthesized polymer, PAMAM-R-PO was able to completely retard pDNA from a charge ratio of 2. The average diameter of PAMAM-R-PO polyplex was found to be 242 nm at a charge ratio of 30. The Zeta-potential value of PAMAM-R-PO polyplex was able to reach 20-30 mV over a charge ratio of 10. PAMAM-R-PO indicated higher cell viability than unmodified PAMAM-R on HeLa and 293 cells because of its hydroxylated amines. Transfection experiments on 293 cells showed that the transfection efficiency of PAMAM-R-PO was found to be 1.5-1.9 times higher than that of PEI25kDa at a charge ratio of 30. The polymer eventually displayed about 2 times greater transfection efficiency than PAMAM-R at the same charge ratio in the absence of serum. Therefore, we concluded that the modification of primary amines of PAMAMR to amino alcohols gives positive effects such as reduced cytotoxicity and enhanced transfection efficiency on 293 cells for gene delivery potency of PAMAM-R.

• Restorative Dentistry and Endodontics
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• 제42권1호
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• pp.39-47
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• 2017

Objectives: Although the coating of surface sealants to dental composite resin may potentially reduce bacterial adhesion, there seems to be little information regarding this issue. This preliminary in vitro study investigated the adhesion of Streptococcus mutans (S. mutans) on the dental composite resins coated with three commercial surface sealants. Materials and Methods: Composite resin (Filtek Z250) discs (8 mm in diameter, 1 mm in thickness) were fabricated in a mold covered with a Mylar strip (control). In group PoGo, the surfaces were polished with PoGo. In groups PS, OG, and FP, the surfaces polished with PoGo were coated with the corresponding surface sealants (PermaSeal, PS; OptiGuard, OG; Fortify Plus, FP). The surfaces of the materials and S. mutans cells were characterized by various methods. S. mutans adhesion to the surfaces was quantitatively evaluated using flow cytometry (n = 9). Results: Group OG achieved the lowest water contact angle among all groups tested (p < 0.001). The cell surface of S. mutans tested showed hydrophobic characteristics. Group PoGo exhibited the greatest bacterial adhesion among all groups tested (p < 0.001). The sealant-coated groups showed statistically similar (groups PS and FP, p > 0.05) or significantly lower (group OG, p < 0.001) bacterial adhesion when compared with the control group. Conclusions: The application of the surface sealants significantly reduced S. mutans adhesion to the composite resin polished with the PoGo.