• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.2
• /
• pp.130-135
• /
• 2000

The characteristics of two different modes of perfusion culture, intermittent and continuous bleedings, were investigated by culturing the hybridoma cells producing von Willebrand Factor (vWF) monoclonal antibody (McAb) in a 15 L bioreactor without clogging the filter. Both culture methods exhibited similar profiles of cell density and metabolite concentrations during the culture period at the cell concentration of around 1${\times}$107 cells/mL. When the perfusion rate was increased, the intermittrnt bleeding culture showed problems of ammonia accumulation and decrease of cell viability. The continuous bleeding culture in terms of nutrient consumption and metabolite production kinetics. But the analysis of specific oxygen consumption rate showed that the specific oxygen consumption rate of intermittent bleeding culture was similar to that of exponential growth phase. The continuous bleeding culture showed higher specific oxygen consumption rate of intermittent bleeding culture. finally we proved the possibility of long-term operation of continuous bleeding culture and produced approximately 40 g of vWF McAb in a 15L bioreactor after one-month operation.

• Journal of Environmental Health Sciences
• /
• v.44 no.3
• /
• pp.244-249
• /
• 2018

Objectives: The purpose of this study was the investigation of bromide and bromate in drinking water of water supply plants, mineral springs and small water supply system located in northern area of Gyeonggi province. Methods: Analytical method was based on EPA 326.0 to use Postcolumn reaction (PCR). The instrument was 887 professional UV/VIS detector IC manufactured in Metrohm. Results: Bromate was detected at $0.5{\sim}2.4{\mu}g/L$ in tap water from 5 water supply plants. These plants were used as disinfection method for sodium hypochlorite and on-site chlorine that causes generate bromate as a by products even if not used ozone. Conclusions: The bromate was detected up to $2.5{\mu}g/Lin$ drinking water in northern Gyeonggi area that showed within $10{\mu}g/L$ for standard of tap water. However, the continuous monitoring of bromate is necessary in drinking water.

• Journal of Korean Society on Water Environment
• /
• v.31 no.2
• /
• pp.87-93
• /
• 2015

In this study, the inhibitory effect of toxic metals was investigated on the activated sludge of the municipal sewage treatment plant. The allowable concentration of toxic metals was also estimated for the stable operation of the biological treatment process. The single and mixture toxicity of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ were evaluated for the activated sludge microorganisms. As a result, nitrifying microorganisms were more susceptible than heterotrophic microorganisms. $IC_{10}$ (Inhibition Concentration of 10%) of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ for the nitrifying microorganisms was 3 mg/L, 7 mg/L and 25 mg/L, respectively. The mixture toxicity showed three times more sensitive than the single toxicity. The concentrations of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ to minimize the inhibitory effect on organic matter removal and nitrification in batch experiments were found to be 1.3 mg/L, 2.5 mg/L and 6.3 mg/L.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.3
• /
• pp.375-384
• /
• 2014

Kenaf (Hibiscus cannabinus L.) was recognized as a potential source of forage. But the domestic cultivation techniques are not set standards yet. So we tested the basic culture techniques during 2012~2013 for getting a high yield and good grade forage production system. The best seeding method for mechanized planting (corn planter used) was hill seeding with $20{\times}20cm$ seeding distance. When we treated hill seeding with $20{\times}20cm$, the yield what we could get was 13,641 kg/10a and it was 32% more than that of conventional practice hill seeding with $20{\times}30cm$ seeding distance. The proper seeding date for getting high yield was May 1. In May 1, the yield per 10a was reached 13,423 kg, and it was 30% more than that of seeding at May 30. More over the crude protein content which was important factor for determinating forage nutritive value was 12.7% and it was higher 1.8% (relatively 16.5% high) than that of May 30 seeding. The most effective herbicide for kenaf was Fluazipof-p-butyl. It's herbicidal rate was 97% and phyto-toxicity was less than 5%. Regional adaptability for Jeollabuk-Do including Imsil Gun, Kochang Gun and Sunchang Gun were identified that the stable cultivation were possible in these area with average yield 12,400 kg/10a and it was about 1.7 times as compared to corn harvest.

• Parasites, Hosts and Diseases
• /
• v.39 no.3
• /
• pp.223-226
• /
• 2001

In South Korea, the north border area has been under vivax malaria epidemic since 1993. However, Jeollabuk-do, which is about 300 kms from the border, has not experienced the same epidemic. 1 investigated a total of 58 notified cases of malaria in Jeollabuk-do in the year 2000. All of the cases had an exposure history in the epidemic area. Among them were 49 ex-soldiers, 3 soldiers who served near the border area and 6 civilians who traveled there. The causal agent of all cases was Plasmodium vivax. Except the civilians, the soldiers and ex-soldiers were aged in their twenty's. In the present study, the incubation period was from 6 to 520 days with a median of 157 days, and the latent onset type (92%) was more prevalent than the early onset type. illness onset of most cases (86%) peaked during the summer season (June to September) despite of variable incubation periods. The time lag for diagnosis ranged from 2 to 42 days with a median of 11 days. Jeollabuk-do has not been an area of epidemic untill now, but incidences have been increasing annually since 1996. In Jeollabuk-do, early diagnosis and treatment can be a feasible disease control measure to prevent spreading from the epidemic area.

• Ecology and Resilient Infrastructure
• /
• v.3 no.4
• /
• pp.263-271
• /
• 2016

This study investigated the hydraulic impact scope and dissolved oxygen (DO) concentration distribution by the micro-bubble aeration in the Juksan Lake located in Asan city in Chungcheongnam-do province. A tracing experiment for hydraulic impact scope was used which constituted a 20% rhodamine solution. A 160 m-guideline was installed in the horizontal direction of the micro-bubble jet flow and the rhodamine concentration, water temperature, and DO concentration were measured at depths of 1 m, 2 m, and 3 m at intervals of 10 m. In the Juksan Lake, the effective range of jet flow discharged by the micro-bubble generator was about 40 m, and after then the jet plume moved up to 80 m to 120 m through the advection and diffusion processes of ambient water. DO concentration in the lake was maintained at 7.4-12.6 mg/L during tracking experiment. The DO of the lake sediments improved from 0.2 mg/L to 8.0 mg/L after applying micro-bubble aeration. In conclusion, the micro-bubble aeration can be an effective technology for the management and improvement of water quality in an agricultural reservoir.

• Journal of Positioning, Navigation, and Timing
• /
• v.10 no.3
• /
• pp.169-177
• /
• 2021

In this paper, we propose a novel global navigation satellite system (GNSS) spoofing detection technique through an array antenna-based direction of arrival (DoA) estimation of satellite and spoofer. Specifically, we consider a sophisticated GNSS spoofing attack scenario where the spoofer can accurately mimic the multiple pseudo-random number (PRN) signals since the spoofer has its own GNSS receiver and knows the location of the target receiver in advance. The target GNSS receiver precisely estimates the DoA of all PRN signals using compressed sensing-based orthogonal matching pursuit (OMP) even with a small number of samples, and it performs spoofing detection from the DoA estimation results of all PRN signals. In addition, considering the initial situation of a sophisticated spoofing attack scenario, we designed the algorithm to have high spoofing detection performance regardless of the relative spoofing signal power. Therefore, we do not consider the assumption in which the power of the spoofing signal is about 3 dB greater than that of the authentic signal. Then, we introduce design parameters to get high true detection probability and low false alarm probability in tandem by considering the condition for the presence of signal sources and the proximity of the DoA between authentic signals. Through computer simulations, we compare the DoA estimation performance between the conventional signal direction estimation method and the OMP algorithm in few samples. Finally, we show in the sophisticated spoofing attack scenario that the proposed spoofing detection technique using OMP-based estimated DoA of all PRN signals outperforms the conventional spoofing detection scheme in terms of true detection and false alarm probability.

• Journal of Food Hygiene and Safety
• /
• v.21 no.3
• /
• pp.153-158
• /
• 2006

The contamination levels of 3-MCPD(3-Monochloropropane-1,2-diol) and 1,3-DCP(1,3-Dichloro-2-Propanol) in soy sauce, sauces was monitored. 105 samples were collected from June 2005 to August 2005 in Gyeonggi-do. 3-MCPD and 1,3-DCP were analyzed by GCMSD. As results, the detection range of 3-MCPD concentration was between 0.02 mg/kg and 0.27 mg/kg in soy sauce. However any samples were not detected with 1,3-DCP. The contaminated rates of 3-MCPD in soy sauce was 17.1%. Soy sauce is the representative vegetable protein hydrolyzed food. The reliable analytical method f3r the toxic 3-MCPD and 1,3-DCP in Soy sauce has been studied. The derivatization studies of 3-MCPD and 1,3-DCP were performed mainly as Silylation with BSA(N,O-Bis(trimethylsilyl)-acetamide). The retention times of derivative, BSA-MCPD, BSA-DCP were 11.30 and 10.12 minutes.

• Journal of Advanced Navigation Technology
• /
• v.26 no.3
• /
• pp.166-171
• /
• 2022

In this paper, we propose an algorithm to improve DoA(direction of arrival) estimation performance of the subspace-based method by generating high robustness correlation matrix of the signals incident on the uniformly linear array antenna. The existing subspace-based DoA estimation method estimates the DoA by obtaining a correlation matrix and dividing it into a signal subspace and a noise subspace. However, the component of the correlation matrix obtained from the low SNR and small number of snapshots inaccurately estimates the signal subspace due to the noise component of the antenna, thereby degrading the DoA estimation performance. Therefore a robust correlation matrix is generated by arranging virtual signal vectors obtained from the existing correlation matrix in a sliding manner. As a result of simulation using MUSIC and ESPRIT, which are representative subspace-based methods,, the computational complexity increased by less than 2.5% compared to the existing correlation matrix, but both MUSIC and ESPRIT based on RMSE 1° showed superior DoA estimation performance with an SNR of 3dB or more.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.19 no.3
• /
• pp.11-27
• /
• 2016

This study is a survey of the flora distributed in Seocheon tidal flat wetland protected area of nearby island (Daejuk-do, Tokki-deung, Muk-do) and Yubo-do Seocheon-gun Chuncheongnam-do, Korea. The numbers of vascular plants were summarized as 231 taxa including 65 families, 167 genera, 204 species, 2 subspecies, 23 varieties and 2 forms. The halophytes were 33 taxa including 15 families, 29 genera, 29 species, 3 varieties and 1 form (Chenopodium album var. stenophyllum, Cnidium japonicum, Juncus haenkei and so on). The rare plants were 4 taxa including Pseudoraphis ukishiba (VU), Belamcanda chinensis (VU), Phacelurus latifolius (LC) and Polygonum bellardii (DD). The Korean endemic plants were 2 taxa including Salix pseudolasiogyne and Forsythia koreana. In the specific plants by floristic region were 24 taxa, a degree I were 19 taxa (Rhodotypos scandens, Zanthoxylum planispinum, Cynodon dactylon and so on), 2 taxa of a degree III (Elymus mollis and Asparagus oligoclonos), 3 taxa of a degree IV (Polygonum bellardii, Wisteria floribunda and Pseudoraphis ukishiba) and degree II, V were not found. The naturalized plants were 40 taxa including Phytolacca americana, Sicyos angulatus, Lactuca scariola and so on. Naturalization rate (NR) was 17.3% of all 231 taxa of vascular plants and urbanization index (UI) was 12.5% of all 321 taxa of naturalized plants. This study was performed to used as a basic data to identify the protected from plant ecosystems of type classify into analyze the vegetation characteristic of based on flora distributed in Yubu-do and nearby islands.