Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Survey of Contaminants of 3-MCPD and 1,3-DCP in Soy Sauce Using GC/MSD

GC/MSD를 이용한 간장 중 3-MCPD와 1,3-DCP의 오염도 조사

  • Kim Myung-Gill (Gyeonggi-do Institute of Health and Environment) ;
  • Kim Young-Sug (Gyeonggi-do Institute of Health and Environment) ;
  • Lee Myung-Jin (Gyeonggi-do Institute of Health and Environment) ;
  • Kim Jae-Kwan (Gyeonggi-do Institute of Health and Environment) ;
  • Kim Kyung-A (Gyeonggi-do Institute of Health and Environment) ;
  • Park Eun-Mi (Gyeonggi-do Institute of Health and Environment) ;
  • Ko Hoan-Uck (Gyeonggi-do Institute of Health and Environment) ;
  • Son Jin-Seok (Gyeonggi-do Institute of Health and Environment)
  • Published : 2006.09.01
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Abstract

The contamination levels of 3-MCPD(3-Monochloropropane-1,2-diol) and 1,3-DCP(1,3-Dichloro-2-Propanol) in soy sauce, sauces was monitored. 105 samples were collected from June 2005 to August 2005 in Gyeonggi-do. 3-MCPD and 1,3-DCP were analyzed by GCMSD. As results, the detection range of 3-MCPD concentration was between 0.02 mg/kg and 0.27 mg/kg in soy sauce. However any samples were not detected with 1,3-DCP. The contaminated rates of 3-MCPD in soy sauce was 17.1%. Soy sauce is the representative vegetable protein hydrolyzed food. The reliable analytical method f3r the toxic 3-MCPD and 1,3-DCP in Soy sauce has been studied. The derivatization studies of 3-MCPD and 1,3-DCP were performed mainly as Silylation with BSA(N,O-Bis(trimethylsilyl)-acetamide). The retention times of derivative, BSA-MCPD, BSA-DCP were 11.30 and 10.12 minutes.

2005년 6월부터 9월까지 경기도내에서 유통되고 있는 간장류 105개에 대하여 CC-MSD을 이용하여 3-MCPD와 1,3-DCP의 오염도를 살펴보았다. 간장에 대한 3-MCPD의 검출률을 살펴보면 17.1%로 나타났고 검출수준은 최저 0.02mg/kg에서 최고 0.27mg/kg이였다. 식품공전에 고시된 잠정허용기준(0.3mg/kg)을 초과하는 검체는 없었고 1,3-DCP는 모든 시료에서 검출되지 않았다. 본 연구에서는 유도체화 방법으로 정량성 있는 BSA를 이용한 Silylation방법을 이용하였는데 이는 유도체 반응 후에 후 처리할 필요가 없어 비교적 간편하게 이용되는 방법이다. BSA-MCPD와 BSA-DCP의 머무름 시간은 11.30min, 10.12min이며 BSA-MCPD와 BSA-DCP의 mass spectra상에서 각각 m/z 147, 219, 239, 103, 116, m/z 93, 165, 95, 151, 185, 149들이 특징적인 이온으로 나타났다. BSA-MCPD의 주된 이온인 m/z 147은 $(CH_3)_3Si-O+=Si(CH_3)_2$로 TMS유도체의 특징적인 이온이다. 또한 TMS유도체의 구조 규명에 손쉽게 사용될 수 있는 $(M-15)^+$ 이온이 BSA-MCPD와 BSA-DCP에서 각각 m/z 239, 185로 확인되었다.

Keywords

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