• Microbiology and Biotechnology Letters
• /
• v.32 no.2
• /
• pp.190-194
• /
• 2004

The gene product of dctD (DctD) activates transcription from the dctA promoter regulatory region by the $\sigma^{54}$ -holoenzyme form ofRNA polymerase ($E\sigma^{54}$ ) in Rhizobium meliloti and R. leguminosarum. The Escherichia coli integration host factor (IHF) stimulated DctD-mediated activation from the dctA promoter regulatory region of R. leguminosarum but not R. meliloti. In the absence of UAS, IHF inhibited DctD-mediated activation from both of these promoter regulatory regions. IHF also inhibited activation from R. leguminosarum dctA by nitrogen regulatory protein C (NtrC), another activator of $E\sigma^{54}$ but not by one which lacks a specific binding site in this promoter regulatory region. IHF, however, stimulated NtrC-mediated activation from the R. meliloti dctA promoter. Upon removal of the UAS, IHF inhibited NtrC-mediated transcription activation from the R. meliloti dctA promoter regulatory region. These data suggest that IHF likely faciliates productive contacts between the activators NtrC or DctD and $E\sigma^{54}$ to stimulate activation from dctA promoter.

• Korean Journal of Microbiology
• /
• v.47 no.2
• /
• pp.174-178
• /
• 2011

In Escherichia coli, DcuS/R two-component system controls fumarate import and utilization related gene expression. To understand the dynamic response of the bacterium DcuS/R two-component system with respect to fumarate concentrations, DcuS/R induced dctA promoter was integrated with GFP reporter protein. Expression monitoring study using recombinant strain showed that dctA promoter was upregulated with 1 mM of fumarate in M9 minimal medium.

• Journal of the Institute of Convergence Signal Processing
• /
• v.4 no.3
• /
• pp.34-40
• /
• 2003

The paper presents a method to detect dissolve shots of video scene change detections in an MPEG compressed domain. The proposed algorithm uses color-R DCT coefficients of Ⅰ, P-frames for a fast operation and accurate detection and a minimum decoding process in MPEG sequences. The paper presents a method to detect dissolve shot for three-dimensional visualization and analysis of Image in order to recognize easily in computer as a human detects accurately shots of scene change. First, Color-R DCT coefficients for 8*8 units are obtained and the features are summed in a row. Second, Four-step analysis are Performed for differences of the sum in the frame sequences. The experimental results showed that the algorithm has better detection performance, such as precision and recall rate, than the existing method using an average for all DC image by performing four step analysis. The algorithm has the advantage of speed, simplicity and accuracy. In addition. it requires less amount of storage.

• Proceedings of the KIEE Conference
• /
• 2008.07a
• /
• pp.2001-2004
• /
• 2008

모든 심전도 압축에서는 압축율과 신호왜곡간의 관계를 다루며 이는 매우 중요하다. 특별히 임상적인 의미를 가진다고 평가되는 5%의 Percent Root mean square Difference(PRD)값을 만족 시키면서 높은 압축율을 얻기 위한 연구는 필수적이다. 본 논문에서는 DCT를 사용하여 심전도 압축을 수행하였을 때, 심전도의 주요한 파라미터인 파형과 RRI(R-R Interval)가 압축율에 따라 어떻게 변화하는지를 평가하고 심전도의 두 가지 주요 파라미터를 진단정보의 왜곡 없이 압축할 수 있는 DCT계수 및 압축율을 도출해 내었다. 실험에는 MIT-BIH ECG Compression Test Database를 사용하였으며 DCT압축을 수행하였을 때 5 % 이하의 PRD를 확보하기 위해서는 81개 샘플에 대하여 평균 4.496 : 1, 최하 3.422 : 1 의 CR을 가지는 것을 확인할 수 있었으며, QRS를 올바르게 검출하는 범위에서의 78개의 샘플에 대하여 평균 CR은 17.3 : 1 최저 CR은 4.6512 : 1 로 나타났다. QRS 검출 한계에서의 RRI 시간왜곡은 평균 3.7149 $\pm$ 4.3147 ms로 나타났으며, 최대 시간왜곡은 13.0256 $\pm$ 14.2035 ms 로 조사되었다.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.25 no.3
• /
• pp.367-373
• /
• 2017

In this paper, a new shift map was proposed to improve the fuel economy of a transmission mounted electric device(TMED) type dual clutch transmission(DCT) plug-in hybrid electric vehicle(PHEV) by considering transmission and motor losses. To construct the shift map, powertrain efficiencies of the engine-DCT-motor were obtained at each gear step. A shift map that provides the highest powertrain efficiency was constructed for the given wheel torque and vehicle speed. Simulation results showed that the fuel economy of the target PHEV can be improved by the new shift map compared with the existing engine optimal operating line(OOL) shift control.

• Journal of Microbiology
• /
• v.38 no.3
• /
• pp.176-179
• /
• 2000

A challenge phage system was used to study the DNA-protein interaction between C4-dicarboxylic acid transport protein D(DCTD) or $\sigma$54, and a $\sigma$54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or $\sigma$54 directed this challenge phage towards lysogency, indicating that DCTD or E$\sigma$54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD(or $\sigma$54) and the dctA promoter region.

• Proceedings of the IEEK Conference
• /
• 2000.09a
• /
• pp.693-696
• /
• 2000

RGB 컬러 좌표계상에서는 각 컬러 성분간의 상관도가 매우 높고, 색을 표현할 때 R, G, B 각 성분에 똑같은 대역폭을 주기 때문에 영상의 압축 관점에서 효율적이지 못하다. 대표적인 영상압축 알고리즘인 MPEG-1,MPEG-2, MPEG-4, H.263, JPEG, JPEG-2000등에서는 압축 효율을 높이기 위해서 YCbCr 컬러 좌표계를 사용하고 있다. 본 논문에서는 좀 더 효율적인 영상 압축을 위해서 새로운 컬러 좌표계를 제안하고자 한다. 제안하는 컬러 좌표계는 DCT 커널에 기반을 두고 있다. DCT 컬러 좌표계는 각각의 커널끼리 직교하며, 빠른 연산이 가능하고, 구조가 간단하다. 실험결과, DCT 컬러 좌표계의 신호들의 분산이 YCbCr 컬러 좌표계의 신호들의 분산보다 작은 경향을 보이며, 동일한 비트율에서 작은 MSE (Mean Square Error)를 가지는 것을 실험적으로 알 수 있다.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2006.10a
• /
• pp.651-652
• /
• 2006

• Journal of Microbiology
• /
• v.40 no.3
• /
• pp.205-210
• /
• 2002

To study interactions between $C_{4}$-dicarboxylic acid transport protein D and E$\sigma$$^{54}$ in the dctA promoter regulatory region, we used the challenge phage system. An ant＇-｀lac fusion was recombined onto the challenge phage, and this ant＇-｀lac fusion along with Pant and the R. meliloti dctA promoter regulatory region were cloned onto a plasmid. The plasmid bearing the ant＇-｀lac fusion was used as a reporter plasmid in a coupled transcription-translation system. Addition of purified $\sigma$$^{54}$ to the coupled system specifically repressed transcription of the plasmid-borne ant＇-｀lac fusion. When DCTD was added along with $\sigma$$^{54}$ to the coupled system, transcription of the ant＇-｀lac fusion was even further repressed, suggesting that DCTD may stabilize closed complexes between E$\sigma$$^{54}$ and the dctA promoter.

• Proceedings of the Korean Society of Medical Physics Conference
• /
• 2005.04a
• /
• pp.71-74
• /
• 2005

To introduce the four-dimensional computed tomography (4DCT, Light Speed RT, General Electric, USA) scanner newly installed in our department and evaluate its feasibility for gated radiotherapy. Respiratory signal measured by real-time position management (RPM$^{\circledR}$, Varian Medical, USA) was recorded in synchronization with the 4DCT scanner. 4DCT data were acquired in axial cine mode and sorted retrospective image based on respiratory phase. PTVs delineated from helical CT and 4DCT images were compared. The PTV delineated from conventional helical CT images was 2 cc larger than that from 4DCT images. Dose in PTV of the plan from retrospective CT was 99.3% (minimum=72.0%, maximum=106.5%) and that of helical CT plan was 95.2% (minimum=24.1%, maximum=106.4%) of prescribed dose. Comparing with DVHs of both plan, the coverage for 4CDT plan was 3.7% improved. It is expected that 4DCT could improve tumor control and reduce radiation toxicity for liver cancer.