• KSBB Journal
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• v.11 no.1
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• pp.8-14
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• 1996

Bacillus subtillis strains with transition state regulator mutations and a spore mutation were developed for the overexpression of apsE and for the enhancement of expression level. Among the many regulator genes, degU and hpr were chosen as a representative positive and negative regulator for the aprE, respectively. Spo II G was used for the construction of asporogeneous strains. All the mutants were constructed from two protease-deleted strain DB104 and the apsE gene was transformed with an integration vector pMK101. DB104(deg$U^h$(32) $his^+$)::pMK101(Cm) and DB104($\Delta$her(Em))::pMKl01(Cm) show 7-fold and about 2-fold increase in aprE expression level, respectively. But the effect of transition state regulator mutation on the aprE expression was diminished when the integrated aprE gene was amplified by the high concentration of chloramphenicol, i. e. 30 $\mu\textrm{g}$/ml. DB104($\Delta$spoIIG(Pm) degUh(32) his+)::pMK101(Cm) and DB104($\Delta$spoIIG(Pm) $\Delta$hpr(Em))::pMK101 double mutant show 10-fold and 3-fold increase in aprE expression level, respectively. The results suggest that sporulation mutation and transition state regulator mutation have independent and additive effect on the aprE expression, and the same gene dosage effect on the transition state regulator mutation was also identified.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2012.11a
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• pp.291-292
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• 2012

Progress in the construction project, the estimated cost of the measure is very important. Use preliminary estimate cost data for the prediction of construction cost. But, preliminary estimate cost data a clear calculation, there is no way. it is rely on the historical data and the past construction data. As a result, a significant difference in the actual construction cost and the predicted cost of the problem occurs. In this study, taking advantage of BIM Cost Prediction for efficient and rapid preliminary estimate BIM for building database through the study preliminary estimate cost data.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2023.05a
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• pp.163-164
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• 2023

The technical proposal bidding system emphasizes the evaluation of the construction company's technical capabilities, with the aim of improving the quality and cost-effectiveness of the construction. However, in the VE analysis and proposal process for technical proposal bidding, the ideas are often reused from similar past construction projects, and the application of new construction technologies/methods is often lacking. Therefore, this study proposes a methodology for managing VE proposal information by integrating it into a BIM model using parametric modeling. This research developed database based on the existing VE proposals and new construction technology/method information from the Korea Institute of Civil Engineering and Building Technology. And by using parametric modeling, the proposed method integrate the VE database with BIM model. The methodology proposed in this study is expected to improve the reliability and effectiveness of VE-related idea proposals in technical proposal bidding.

• Journal of Korea Multimedia Society
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• v.14 no.11
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• pp.1450-1457
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• 2011

Currently most of leak detection monitoring systems use digital maps with paying royalties, and this increases the cost of system construction and financial burdens on local self-governing bodies that manage such systems. Moreover, they have inefficiencies in repair and maintenance, functional expansion, and compatibility with other systems. Thus, this study developed a waterworks pipe network monitoring system that pursues low cost and high efficiency using general-purpose maps on the Internet such as google maps. As this system uses highly compatible free maps, it costs less in construction and its hardware requirements are lower than existing systems, and consequently, overall monitoring performance is enhanced and the cost of construction goes down sharply. This study also proposed a method for pipeline DB construction, which can be started together with the construction of the monitoring system, in order to improve the field applicability of the system.

• Proceedings of the KSR Conference
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• 2004.06a
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• pp.1132-1135
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• 2004

Recently. our construction industry is using 4D-CAD system but the public Database (DB) for the 4D-CAD system is insufficiently built. It is difficult to connect 4D-CAD system information such as schedule, drawings. Therefore this study suggest a methodology to link the 4D-CAD system information with work breakdown structure(WBS) and the methodology suggested in this study was applied to a 4D-CAD system

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.04a
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• pp.625-626
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• 2009

• Smart Media Journal
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• v.12 no.11
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• pp.154-164
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• 2023

The Construction Technology Information System collects, processes, and provides the public with free of charge 16 types of construction technology-related texts or index information, such as construction standards, construction practices, and construction reports, required at construction sites to enhance domestic construction technology. This study judged that user satisfaction could be increased if the budget was allocated first to the most effective construction technology information DB establishment. For each type of construction technology information, the level of satisfaction felt by the population for the quantity provided and the value of use according to the acquisition and utilization of information were investigated. Reliability between independent variables and dependent variables through a multiple regression analysis process to identify how the demographic characteristics of the population affect user satisfaction and the factors that affect the use value of information for each type of construction technology information. and the correlation was analyzed. The correlation between the demographic characteristics of respondents and users' satisfaction with the provided quantity was generally low, but construction experience, age, etc. were found to have an effect on satisfaction with the provided quantity. In particular, among the demographic measurement items of respondents, the more construction experience they had, the higher their satisfaction level with the quantity provided of low-level technical standards, and it was analyzed that older age had an impact on satisfaction with the quantity provided for small and medium-sized business support information.

• Journal of Korean Society of Steel Construction
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• v.26 no.5
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• pp.385-396
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• 2014

Traditionally, domestic steel design and construction practice has provided extra shear studs to moment frame beams even when they are designed as non-composite beams. In the 1994 Northridge earthquake, connection damage initiated from the beam bottom flange side was prevalent. The upward moving of the neutral axis due to the composite action between steel beam and floor deck was speculated to be one of the critical causes. In this study, full-scale seismic testing was conducted to investigate the side effects of the composite action in steel seismic moment frames. The specimen PN700-C, designed following the domestic connection and floor deck details, exhibited significant upward shift of the neutral axis under sagging (or positive) moment, thus producing high strain demand on the bottom flange, and showed a poor seismic performance because of brittle fracture of the beam bottom flange at 3% story drift. The specimen DB700-C, designed by using RBS connection and with the details of minimized floor composite action, exhibited superior seismic performance, without experiencing any fracture or concrete crushing, almost identical to the bare steel counterpart (specimen DB700-NC). The results of this study clearly indicate that the beams and connections in seismic steel moment frames should be constructed to minimize the composite action of a floor deck if possible.

• Journal of the Korean Society for information Management
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• v.22 no.3 s.57
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• pp.183-199
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• 2005

This paper explains that socio-cultural contexts affect the transition in scholarly communication. Observing the process of introducing the open access model to the field of medicine in Korea, this paper found that the first, associations of medical societies considered a quality control system very important in the construction of an open access based fulltext DB, the second, there was an medical journal DB with a quality control procedure, and the third, many medical societies provided their journals fulltext to the public on their homepages. Consequently, this paper found that these factors had an effect on the process of transition in scholarly communication in the field of medicine in Korea.

• The Journal of Bigdata
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• v.4 no.2
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• pp.195-205
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• 2019

Linked Open Data (LOD) is rated as the best of any kind of data disclosure, and allows you to search related data by linking them in a standard format across the Internet. There is an increasing number of cases in which relevant data are constructed in the LOD form in the global environment, but in the domestic healthcare sector, the disclosure of data in the form of LOD is still at the beginning stage. In this paper, we introduce a case of LOD platform construction that provides services by linking domestic and international related data by LOD method, based on the data of Korean medical research paper data and health care big data linkage platform. Linking all data from each DB into an LOD requires a lot of time and effort, and is basically an infrastructure task that government or public institutions should be in charge of rather than the private sector. In this study, ten domestic and foreign LOD sites were linked with only a portion of each DB, enabling users to link data from various domestic and foreign organizations in a convenient manner.