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Distribution of Tetracycline Resistance Genes in Pathogenic Bacteria Isolated from Cultured Olive Flounder (Paralichthys olivaceus) in Jeju in 2016 (2016년도 제주지역 양식 넙치(Paralichthys olivaceus)에서 분리된 어병세균의 tetracycline 내성유전자 분포)

  • LEE, Da-Won;JUN, Lyu-Jin;JEONG, Joon-Bum
    • Journal of Fisheries and Marine Sciences Education
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    • v.29 no.3
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    • pp.834-846
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    • 2017
  • Aquaculture practices to ensure greater production, such as high density breeding and excessive feeding, are become stressors that raise the prevalence of diseases. Accordingly, increasingly large volumes of antibiotics are used more frequently each year. Long term use antibiotics can generate resistant bacteria, which interrupt treatments and cause a potential transfer to human bodies. Thus, antibiotic resistance is of importance in public health. Tetracycline (Tc) is one of the typical medicines used in the aquaculture drugs, which has a wide range of application including gram-positive and gram-negative bacteria. In the examination of 153 strains isolated from olive flounder (Paralichthys olivaceus) farms located in Jeju in 2016, it turned out that a total of 84 strains were resistant to Tc or oxytetracycline (OTC). The extent to which the strains are resistant to Tc and OTC was confirmed through MIC test, mostly within the range of 25 to $100{\mu}g/m{\ell}$. Twelve different types of tet genes were detected using single and multiplex PCR in the 84 Tc-resistant strains. The PCR was used to find tet(K), tet(M), tet(O), and tet(S), which are known to exist primarily in gram positive strains. According to the results, - tet(S) is the most dominant gene in 49 strains of Streptococcus parauberis, accounting for 63.2%. And there were two strains that have two different types of resistant genes. The multiplex PCR was used to detect tet(A), tet(B), tet(C), tet(D), tet(E), and tet(G), which are commonly found in gram-negative strains. Each of tet(B), tet(D), and tet(B)&(M) was found in a strain presumed to be Vibrio sp., and only tet(D) was found in 10 Edwardsiella tarda strains.

A Study on the Control of the Exhaust CO from Gas Boiler (가스보일러 일산화탄소 제어에 관한 연구)

  • Jo Young-Do;Choi Kyoung-Suhk;Kim Ji-Yoon;Kim Chang-Yeon
    • Journal of the Korean Institute of Gas
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    • v.5 no.1
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    • pp.7-14
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    • 2001
  • In this work, the chemical composition of the exhaust gas from domestic gas boiler has been analysed in the point of thermodynamics and CO sensor has been characterized. We proposed that the combustion condition can be estimated by the exhaust gas composition, i.e., the excess air ratio and combustion temperature can be calculated simply by the measurement of the $O_{2}$ fraction and $H_{2}/CO$ in the exhaust gas. By analyse the on site situation domestic boiler, the excess air ratio is about $55\~110\%$. Therefore, the CO may be produced in domestic gas boiler by luminous(yellow) flames rapidly lose heat by radiation, turbulent flames may be partially quenched by the action of steep velocity gradients, and flames burning very close to a cold wall may be partial1y quenched by heat conductivity to the wall. The output voltage of CO sensor is lineally depend on the CO and $H_{2}$concentration. And the exhaust CO from boiler can be reduced by closed loop control with CO sensor

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A Case Study for Efficient Blended Learning Management (효율적인 혼합형 학습 운영을 위한 사례연구)

  • Kwon, Oh-Young
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.2 no.1
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    • pp.52-57
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    • 2010
  • Using the Operating Systems course that is offered by online, a blended learning mixed up with face-to-face lecture and e-learning for O.S. course has been carried out. In order to find a efficient management way of the blended learning, we build up two groups: one group named 01 takes a class which consists of two hours face-to-face lecture and one hour online study per week and the other group named 02 takes a class which consists of two hours online study and one hour face-to-face lecture. According to the result of a mid-term examination, the Cohen's d between two groups is 0.165. It means the small effect size. The 01 group has higer average and smaller variance than 02 group. However, 02 group has more students who earn high score than 01 group. In conclusion, if students can well carry out the self-regulated learning, then the blended learning mixed up with 02 group style is suitable. Otherwise, face-to-face lecture or the blended learning like 01 group style is suitable.

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Simultaneous Measurement of Ultrasonic Velocity and Thickness of Isotropic and Homogeneous Solids Using Two Transducers (두개의 탐촉자를 사용한 등방성 균일 고체의 초음파 속도 및 두께 동시 측정법)

  • Lee, Jeong-Ki;Kwon, Jin-O;Kim, Young-H.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.19 no.5
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    • pp.363-368
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    • 1999
  • Ultrasonic pulse-echo methods measuring the transit time through specimens have been widely used in determination of ultrasonic velocity and thickness of specimens. Usually, to determine the velocity of the ultrasonic. the transit time of the ultrasonic pulse through specimen is measured by using the ultrasonic measuring equipment such as the oscilloscope including ultrasonic pulser/receiver and the thickness of the specimen is measured by using the length measuring instrument such as micrometer or vernier calipers etc., i. e. each parameter is measured by using each measuring method. In the case of the measuring the thickness of a specimen by using the ultrasonics. the ultrasonic equipments, which measure the thickness, such as the ultrasonic thickness gauge must be calibrated by using the reference block of which the ultrasonic velocity is known beforehand. In the present work, we proposed a new method for simultaneous measurement of ultrasonic velocity and thickness without reference blocks. Experimental results for several specimens show that proposed method have good agreements with those by traditional ultrasonic method.

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Combined Effect of Irradiation and Ageing Condition on Physicochemical and Microbial Quality of Hanwoo Eye of Round

  • Yim, Dong-Gyun;Jo, Cheorun;Kim, Hyun-Joo;Cha, Ju-Su;Kim, Hyun Cheol;Nam, Ki-Chang
    • Food Science of Animal Resources
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    • v.35 no.3
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    • pp.406-412
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    • 2015
  • The combined effects of electron-beam irradiation and ageing of beef were examined. The irradiated samples at dose of 0 or 2 kGy were kept and analyzed for the microbial growth, shear values, meat color, and nucleotide-related flavor compounds at different ageing temperatures (2, 10, or 25℃) for 8 d. The irradiation effect on inactivation of foodborne pathogens was also investigated. The population of Listeria monocytogenes and E. coli O157:H7 inoculated in beef samples decreased in proportion to the irradiation dose, showing D10 values of 0.66 and 0.65 kGy respectively. The irradiated beef eye of round had lower number of total aerobic bacteria (TAB) than nonirradiated one during the storage, but the TAB increased with higher ageing temperature (p<0.05). Especially, TAB increased sharply in non-irradiated samples aged at 25℃ after 4 d (p<0.05). With increasing ageing temperature and ageing time, shear force values decreased (p<0.05). The color a* values of the irradiated beef were lower than those of the non-irradiated throughout the ageing period (p<0.05). As ageing time and temperature increased, the amounts of inosine monophosphate decreased and the hypoxanthine increased (p<0.05). Relatively high ageing temperature could be used at irradiated beef eye of round to shorten the ageing time.

The Effect of Fiber Stacking Angle on the Relationship Between Fatigue Crack and Delamination Behavior in a Hybrid Composite Materials (하이브리드 복합재료의 섬유배향각이 피로균열 및 층간분리 거동의 관계에 미치는 영향)

  • Song, Sam-Hong;Kim, Cheol-Woong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.3
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    • pp.281-288
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    • 2004
  • The hybrid composite material (Al/GFRP laminates) are applied to the fuselage and wing in a aircraft. Therefore, Al/GFRP laminates suffer from the cyclic bending moments. This study was to evaluate the effect of fiber stacking angle on the fatigue crack propagation and delamination behavior using the relationship between crack growth rate (da/dN) and stress intensity factor range (ΔK) in Al/GFRP laminates under cyclic bending moment. The variable delamination growth behavior in case of three different type of fiber orientations, i.e., [Al/O$_2$/Al], [Al/+45$_2$/Al] and [Al/90$_2$/Al] at the interface of Al layer and glass fiber layer was measured by ultrasonic C-scan images. As results of this study, It represent that the delamination shape should turns out to have more effective characteristics on the fiber stacking angle. The extension of the delamination zone in case of [Al/+45$_2$/Al] and [Al/90$_2$/Al] were not formed along the fatigue crack profile. The shape of delamination zone depend on fiber stacking angle and the variable type with the delamination contour decreased non-linearly toward the crack tip at the Al layer.

Automatically Converting HTML Documents with Similar Pattern into XML Documents (유사 패턴을 갖는 HTML 문서의 XML 자동 변환)

  • O, Geum-Yong;Hwang, In-Jun
    • The KIPS Transactions:PartD
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    • v.9D no.3
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    • pp.355-364
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    • 2002
  • Recently, WWW(World Wide Web) has become a source of a large amount of information, and is now recognized not only as an information-sharing tool, but also as an information repository. Currently, the majority of documents on the web were created using HTML(Hypertext Markup Language). Although HTML is simple and easy to learn, its inherent lack of describing document structure makes it difficult to retrieve information effectively. One possible solution would be to convert such HTML documents into XML (extensible Markup Language) documents. This is a standard markup language for exchanging data on the web. It can describe a document structure freely by defining its own DTD (Document Type Definition). This makes it possible to integrate, store, and retrieve data on the web efficiently In this paper, we will propose a converter that automatically converts HTML documents with similar pattern into XML documents by analyzing the document structure and recognizing its path information.

Safety Evaluation of Bifidobacterium breve IDCC4401 Isolated from Infant Feces for Use as a Commercial Probiotic

  • Choi, In Young;Kim, Jinhee;Kim, Su-Hyeon;Ban, O-Hyun;Yang, Jungwoo;Park, Mi-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.949-955
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    • 2021
  • Previously, our research group isolated Bifidobacterium breve IDCC4401 from infant feces as a potential probiotic. For this study, we evaluated the safety of B. breve IDCC4401 using genomic and phenotypic analyses. Whole genome sequencing was performed to identify genomic characteristics and investigate the potential presence of genes encoding virulence, antibiotic resistance, and mobile genetic elements. Phenotypic analyses including antibiotic susceptibility, enzyme activity, production of biogenic amines (BAs), and proportion of D-/L-lactate were evaluated using E-test, API ZYM test, high-performance liquid chromatography (HPLC), and D-/L-lactic acid assay respectively. The genome of B. breve IDCC4401 consists of 2,426,499 bp with a GC content of 58.70% and 2,016 coding regions. Confirmation of the genome as B. breve was provided by its 98.93% similarity with B. breve DSM20213. Furthermore, B. breve IDCC4401 genes encoding virulence and antibiotic resistance were not identified. Although B. breve IDCC4401 showed antibiotic resistance against vancomycin, we confirmed that this was an intrinsic feature since the antibiotic resistance gene was not present. B. breve IDCC4401 showed leucine arylamidase, cystine arylamidase, α-galactosidase, β-galactosidase, and α-glucosidase activities, whereas it did not show production of harmful enzymes such as β-glucosidase and β-glucuronidase. In addition, B. breve IDCC4401 did not produce any tyramine, histamine, putrescine, cadaverine, or 2-phenethylamine, which are frequently detected BAs during fermentation. B. breve IDCC4401 produced 95.08% of L-lactate and 4.92% of D-lactate. Therefore, our findings demonstrate the safety of B. breve IDCC 4401 as a potential probiotic for use in the food industry.

Development of Precision Agricultural Machine Education Program (정밀 농업기계 교육프로그램의 발전)

  • Hong, S.J.;Kim, D.E.;Kang, D.H.;Kim, J.J.;Kang, J.G.;Chung, S.O.;Mo, C.Y.;Ryu, D.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.2
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    • pp.115-122
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    • 2020
  • In Korea, the agricultural machinery market has been generally on the rise, and particularly the demand for the diverse agricultural machine is increasing due to the radical changes in agriculture, such as a high supply of the advanced and automated agricultural machine and an increase in aged or female farmer population. Therefore, this study analyzes the technical trends in the precision agricultural machine domestically and globally to guide the direction of development of the ICT-based machine. The investigation of the precision agricultural machine in this study focuses on the production technology through analyzing the trends in sensor-related technology, the decision-making research, variable treatment technology, and academic publication. The result shows that information processing technology including the sensor and the decision-making requires various measurement factors and the established technologies are continually being developed.

Spatially-resolved Photoluminescence Studies on Intermixing Effect of InGaAs Quantum Dot Structures Formed by AlAs Wet Oxidation and Thermal Annealing (AlAs 습식산화와 열처리로 인한 InGaAs 양자점 레이저 구조의 Intermixing효과에 관한 공간 분해 광학적 특성)

  • Hwang J.S.;Kwon B.J.;Kwack H.S.;Choi J.W.;Choi Y.H.;Cho N.K.;Cheon H.S.;Cho W.C.;Song J.D.;Choi W.J.;Lee J.I.
    • Journal of the Korean Vacuum Society
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    • v.15 no.2
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    • pp.201-208
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    • 2006
  • Optical characteristics of InGaAs quantum dot (QD) laser structures with an Al native oxide (AlOx) layer as a current-blocking layer were studied by means of photoluminescence (PL), PL excitation, and spatially-resolved micro-PL techniques. The InGaAs QD samples were first grown by molecular-beam epitaxy (MBE), and then prepared by wet oxidation and thermal annealing techniques. For the InGaAs QD structures treated by the wet oxidation and thermal annealing processes, a broad PL emission due to the intermixing effect of the AlOx layer was observed at PL emission energy higher than that of the non-intermixed region. We observed a dominant InGaAs QD emission at about 1.1 eV in the non-oxide AlAs region, while InGaAs QD-related emissions at about 1.16 eV and $1.18{\sim}1.20eV$ were observed for the AlOx and the SiNx regions, respectively. We conclude that the intermixing effect of the InGaAs QD region under an AlOx layer is stronger than that of the InGaAs QD region under a non-oxided AlAs layer.