• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.35-35
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• 2003

We, for the first time, reported molecular sequences of large subunit ribosomal DNA Dl-D3 region of A. hiranoi, A. leei and A. satoanum hitherto unreported. In addition, this study presented the full-length sequences of A. affine, A. fraterculus, A. catenella and A. tamarense occurring in Korean coastal waters. In total, 17 Alexandrium morphospecies were subjected to the phylogenetic analysis using the Maximum-likelihood (ML) method. The alignment result of sequences of A. hiranoi and A. pseudogonyaulax showed that there were only two substitutions without length heterogeneity implying their genetic affiliation. In ML tree, A. leei formed a deeply diverging branch probably because of the accelerated evolutionary rate, and its phylogenetic position was so ambiguous to resolve the phylogenetic relationship to the residual taxa. An A. satoanum culture showing morphological variation in the sulcal plate formed an independent divergent branch with consistent sister relationship to A. hiranoi/A. pseudogonyaulax clade supported by the high posterior probability (PP) value. Blast search in GenBank showed the sequence data of A. affine, A. fraterculus, A. catenella and A. tamarense corresponded to their morphological species designation. In ML tree, Alexandrium species were commonly split into four main clades. The inter-clade relationships were not clear and usually supported by the week PP values. In general, the sulcal plate of Alexandrium species seemed to reflect the true phylogeny at the main clade level, and the connection between the 1 and the apical pore complex seemed to reflect the phylogeny at the subclade level.

• Childhood Kidney Diseases
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• v.21 no.1
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• pp.21-25
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• 2017

Severe hypercalcemia is rarely encountered in children, even though serum calcium concentrations above 15-16 mg/dL could be life-threatening. We present a patient having severe hypercalcemia and azotemia. A 14-year-old boy with no significant past medical history was referred to our hospital with hypercalcemia and azotemia. Laboratory and imaging studies excluded hyperparathyroidism and solid tumor. Other laboratory findings including a peripheral blood profile were unremarkable. His hypercalcemia was not improved with massive hydration, diuretics, or even hemodialysis, but noticeably reversed with administration of calcitonin. A bone marrow biopsy performed to rule out the possibility of hematological malignancy revealed acute lymphoblastic leukemia. His hypercalcemia and azotemia resolved shortly after initiation of induction chemotherapy. Results in this patient indicate that a hematological malignancy could present with severe hypercalcemia even though blast cells have not appeared in the peripheral blood. Therefore, extensive evaluation to determine the cause of hypercalcemia is necessary. Additionally, appropriate treatment, viz., hydration or administration of calcitonin is important to prevent complications of severe hypercalcemia, including renal failure and nephrocalcinosis.

• Journal of the Korean Institute of Gas
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• v.20 no.5
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• pp.89-95
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• 2016

Keeping the gas pipelines in the common utility tunnel is useful because it has a lower risk of corrosion than conventional burial, and can prevent from excavating construction. But, explosions in common utility tunnels can cause greater damage from the blast overpressure compared to outdoor explosions, due to nature of the confined environment. Despite this fact, however, research on common utility tunnels has been limited to fire hazard and little has been studied on the dangers of explosions. This study developed scenarios of methane gas explosion caused by gas leak from gas piping within the common utility tunnel followed by unknown ignition; the study then calculated the extent of the impact of the explosion on the facilities above, and suggested the needs for designing additional safety measures. Two scenarios were selected per operating condition of safety devices and the consequence analysis was carried out with FLACS, one of the CFD tools for explosion simulation. The overpressures for all scenarios are substantial enough to completely destroy most of the buildings. In addition, we have provided additional measures to secure safety especially reducing incident frequency.

• Biomedical Science Letters
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• v.10 no.1
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• pp.23-29
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• 2004

Nebulin is a (Mr 600∼900 kDa) large actin-binding protein specific to skeletal muscle and thought to act as a molecular template that regulates the length of thin filaments. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Recently, full-length nebulin mRNA transcripts have been detected in heart muscle, but at lower levels than in skeletal muscle. Nebulin expression also was detected in the kidney, eye, and otic canal, suggesting that nebulin isoforms may also be expressed in these organs. We have searched for nebulin isoforms in brain of human using PCR and Northern blot. Here, we provide evidence that nebulin mRNA transcripts are expressed in brain. Seven nebulin isoforms (B, C, D, E, F, G and H form) are obtained in human skeletal muscle and four isoforms (B, C, G and H form) in human brain cDNA library. We cloned the 1.3 kb of nebulin fragment from human adult brain library by PCR. The identity of the PCR product was confirmed by sequence analysis. The partial brain nebulin sequence was 99% identical to the skeletal muscle cDNA as determined by Blast alignment. It contains two simple-repeats HR1, HR2 and linker-repeats exon l35∼143 except exon 140. It was different from skeletal muscle B form, which contain HR1 and HR8. These data suggest that nebulin isoform diversity occurs even more extensively than previously known, likely contributing to the distinct thin filament architecture of different striated muscles.

• Computers and Concrete
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• v.12 no.6
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• pp.785-802
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• 2013

Ready-mixed soil material, known as a kind of controlled low-strength material, is a new way of soil cement combination. It can be used as backfill materials. In this paper, artificial neural network and nonlinear regression approach were applied to predict the compressive strength of ready-mixed soil material containing Portland cement, slag, sand, and soil in mixture. The data used for analyzing were obtained from our testing program. In the experiment, we carried out a mix design with three proportions of sand to soil (e.g., 6:4, 5:5, and 4:6). In addition, blast furnace slag partially replaced cement to improve workability, whereas the water-to-binder ratio was fixed. Testing was conducted on samples to estimate its engineering properties as per ASTM such as flowability, strength, and pulse velocity. Based on testing data, the empirical pulse velocity-strength correlation was established by regression method. Next, three topologies of neural network were developed to predict the strength, namely ANN-I, ANN-II, and ANN-III. The first two models are back-propagation feed-forward networks, and the other one is radial basis neural network. The results show that the compressive strength of ready-mixed soil material can be well-predicted from neural networks. Among all currently proposed neural network models, the ANN-I gives the best prediction because it is closest to the actual strength. Moreover, considering combination of pulse velocity and other factors, viz. curing time, and material contents in mixture, the proposed neural networks offer better evaluation than interpolated from pulse velocity only.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.12
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• pp.1703-1709
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• 2008

As genetic markers, single nucleotide polymorphisms (SNP) are very appropriate for the development of genetic tests for economic traits in livestock. Several microsatellite markers have been identified as useful markers for the genetic improvement of Hanwoo. Among those markers, ILSTS035 was recently mapped at a similar position with four SNPs (AH1_11, AH1_9, 31465_446, and 12273_165) in a linkage map of EST-based SNP in BAT6. Among the four SNPs, two SNPs (31465_446 and 12273_165) were analyzed using BLAST at the NCBI web site. The sequences including the 12273_165 SNP were identified at the intron region within the LOC534614 gene on the gene sequence map (Bos taurus NCBI Map view, build 3.1). The LOC534614 gene represents a protein similar to myosin heavy chain, fat skeletal muscle, embryonic isoform 1 in the dog, and myosin_1 (Myosin heavy chain D) in Macaca mulatta. In cattle, the myosin heavy chain was associated with muscle development. The phenotypic data for growth and carcass traits in the 415 animals were analyzed by the mixed ANCOVA (analysis of covariance) linear model using PROC GLM module in SAS v9.1. By the genotyping of Hanwoo individuals (n = 415) to evaluate the association of SNP with growth and carcass traits, it was shown that the 12273_165 SNP region within LOC534614 may be a candidate marker for growth. The results of the statistical analyses suggested that the genotype of the 12273_165 SNP significantly affected birth weight, weight of the cattle at 24 months of age, average daily gain and carcass cold weight (p<0.05). Consequently, the 12273_165 SNP polymorphisms at the LOC534614 gene may be associated with growth in Hanwoo, and functional validation of polymorphisms in LOC534614 should be performed in the future.

• Korean Journal of Clinical Pharmacy
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• v.19 no.2
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• pp.153-158
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• 2009

Background: Patients with chronic myeloid leukemia(CML) have different health status according to their disease conditions such as chronic phase(CP), accelerated phase(AP), blast crisis(BC), stage with MCyR(Major Cytogenetic Response); therefore, every patient has different quality of life related to their disease condition. Objectives: To measure the quality of life, this study compared and analyzed the utility weight in patients with CML. This study also evaluated the utility weight in order to view comparisons between the quality of life in a patient with CML to a patient with diabetes, which is a representative chronic disease. Methods: The disease scenario described 5 symptoms of the CP, AP, BC of the CML, the CML which gets the MCyR and the diabetes. Utility weight was developed using the EQ-5D method. All statistical data were analyzed by STATA 10.0 Results: 57 nurses(95%) out of 60 answered the questionnaire. In CP, the utility weight was 0.7946. In AP, it was 0.5301. and in BC, it was -0.2793. Survey data indicate that the worse the condition of a disease, the lower the utility weight. In case of the CML which gets the MCyR, the result was 0.7731(95% CI : 0.7384 - 0.8079). The general diabetes which has no complicating disease, the utility weight was 0.7481(95% CI : 0.6983 - 0.7978). Based on the result, it is evident that those with MCyR are not significantly different from people with general diabetes (p=0.4096) in views of the quality of life.

• Journal of the Korean Ceramic Society
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• v.44 no.1 s.296
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• pp.6-11
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• 2007

The rheological properties of slag cement pastes by effect of particle size distribution of binder were investigated using a Rheostress 1 rheometer (Haake) with a cylindrical spindle and the relationship between fluidity particle size distribution using the Rosin-Rammler equation. Samples are combined the two types of slag powder and OPC, fine slag particles sized Elaine specific surface area $8,000cm^2/g$, coarse slag particles sized Elaine specific surface area $2,000cm^2/g$, intermediate OPC particles $3,450cm^2/g$, used to search for the combination that would yield the best quality product. The all flow curves which were measured by rheometer showed hysterisis and could be classified into 4 types. When the combination was based on a ratio of 15-20 vol% fine particles, 40-50 vol% intermediate particles, 30-40 vol% coarse particles of the total volume, a high fluidity and low yield-strength was achieved. The Rosin-Rammler function can explain aboved correlation flow curve types. On type 1, the n-value had a correlation with plastic viscosity however the blend of type 2 and 3 showed consistent n-value regardless of plastic viscosity. In addition, the blend in type 4 tended to a rise in fluidity according to the increase of the n-value.

• Journal of Navigation and Port Research
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• v.29 no.8 s.104
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• pp.735-740
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• 2005

In this paper, we designed a wedge type rail-clamp which can protect container crane from sudden wind blast with constant clamping force regardless of the operating period. When we design wedge type rail clamp, it is important to determine an angle of wedge which prevent rotating of jaw and for smooth operation when wind blows. Therefore, this paper suggest a process to decide an angle of wedge within proper range obtained by experimental analysis as well as FEA of the wedge type rail clamp. A model with $6^{\circ}$ wedge angle is the most proper model to use in rail clamp bemuse it generated satisfactory clamping force and rotating angle underdesign specification.

• Journal of the Korean Recycled Construction Resources Institute
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• v.5 no.4
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• pp.466-473
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• 2017

RC (Reinforced Concrete) member has varying service life due to varying diffusion characteristics with loading conditions even if it is exposed to constant exterior conditions. In the paper, quantitative parameters are obtained through adopting the previous results for effects of compressive, tensile, and cold joint on chloride diffusion in OPC (Ordinary Portland Cement) and GGBFS (Ground Granulated Blast Furnace Slag) concrete. Service life is evaluated in RC simple beam with 10.0m of span through increasing loading from self weight (2.5kN/m) to the loading to cracking moment (5.5kN/m). In OPC concrete without cold joint, service life changes to 89.4% for tensile region and 101% for compressive region with loadings while GGBFS concrete has 80.0% and 106%, respectively. For cold joint area, GGBFS concrete shows much reduced service life to 82~80% in compressive region and 69~61% in tensile region, which is caused by the lower diffusion in normal condition but relatively higher increasing cold joint effect than OPC concrete.