• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 1998년도 가을 학술발표논문집 Vol.25 No.2 (3)
• /
• pp.424-426
• /
• 1998

공간정보의 효율적 공유를 위해 인터넷 기반 GIS소프트웨어 개발 및 응용과 관련된 연구가 활발히 진행 중에 있다. 여러 인터넷 GIS의 기본적인 요구사항 및 현재까지 개발모델과 문제점을 살펴보고, 표준 인터넷 기술을 기반으로 최근 웹 기술 표준 동향을 포함한, OpenGIS상호 운용성이 지원되는 인터넷 GIS기본 구조를 제시하고자 한다. 표준화될 인터넷 GIS 속도 향상과 TCP/IP상의 보안문제가 해결되어야 하고, OpenGIS에서 구성하고 있는 공간 데이터 공유를 위한 표준 사양을 준수할 뿐 아니라 클라이언트/서버의 부하가 최적화된 구조여야한다. 특히 웹 중심의 각종 인터넷 기술들, 즉 HTTP NG. XML, SSL등의 표준 기술이 함께 적용되어야 한다. 새로운 인프라구조는 GIS D/B에 포함된 확장된 (Enhanced) HTTP/MAP 서버와 클라이언트로 구성된다. MAP클라이언트는 MIME-TYPE 에 따라 GIS데이터를 표시할 수 있는 윈도우 환경으로 변환되며 GIS 데이터셋은 XML을 기반으로 하는 MapML(Mapping Makup Language)를 통해 형식을 정한다. 클라이언트가 MapML 토큐먼트를 통해 정의된 구획의 레이어와 벡터 데이터를 요청하고, Map서버는GIS D/B에서 WKB 혹은 소위 VML 형태로 추출하여 클라이언트로 보내주게 된다. 주어진 구획은 MapML로 정의된 속성들을 통해 각종 부가 정보를 열람할 수 있다. MAP은 HTTP와 같은 형태로 동작하므로 전자인증, 암호화를 통한 GIS정보 보안, 클라이언트와 서버 부하의 효율적인 분배 XML을 통한 다양한 GIS속성표현이 가능하다. 본 구조는 Apache +Amiya + Crass D/B+ MapML 환경에서 구현되고 있다.팔일 전송 기법을 각각 제시하고 실험을 통해 이들의 특성을 비교분석하였다.미에서 uronic acid 함량이 두 배 이상으로 나타났다. 흑미의 uronic acid 함량이 가장 많이 용출된 분획은 sodium hydroxide 부분으로서 hemicellulose구조가 polyuronic acid의 형태인 것으로 사료된다. 추출획분의 구성단당은 여러 곡물연구의 보고와 유사하게 glucose, arabinose, xylose 함량이 대체로 높게 나타났다. 점미가 수가용성분에서 goucose대비 용출함량이 고르게 나타나는 경향을 보였고 흑미는 알칼리가용분에서 glucose가 상당량(0.68%) 포함되고 있음을 보여주었고 arabinose(0.68%), xylose(0.05%)도 다른 종류에 비해서 다량 함유한 것으로 나타났다. 흑미는 총식이섬유 함량이 높고 pectic substances, hemicellulose, uronic acid 함량이 높아서 콜레스테롤 저하 등의 효과가 기대되며 고섬유식품으로서 조리 특성 연구가 필요한 것으로 사료된다.리하였다. 얻어진 소견(所見)은 다음과 같았다. 1. 모년령(母年齡), 임신회수(姙娠回數), 임신기간(姙娠其間), 출산시체중등(出産時體重等)의 제요인(諸要因)은 주산기사망(周産基死亡)에 대(對)하여 통계적(統計的)으로 유의(有意)한 영향을 미치고 있어 $25{\sim}29$세(歲)의 연령군에서, 2번째 임신과 2번째의 출산에서 그리고 만삭의 임신 기간에, 출산시체중(出産時體重) $3.50{\sim}3.99kg$사이의 아이에서 그 주산기사망률(周産基死亡率)이 각각 가장 낮았다. 2. 사산(死産)과 초생아사망(初生兒死亡)을 구분(區分)하여 고려해 볼때 사산(死産)은 모성(母

• 농업과학연구
• /
• 제34권2호
• /
• pp.99-109
• /
• 2007

섬유소 분해균 Cellulomonas sp. CS 1-1에 대하여 종 수준으로 분류학적 위치를 규명하기 위하여 7개 type strain 균주와 함께 생리적 및 생화학적 특성을 조사하고 DNA 상등성 및 지방산의 조성 등을 분석하여 비교한 결과, CS 1-1의 콜로니 형태는 circular, entire, smooth, convex하며, 담황색을 띤 $0.3{\sim}0.5{\times}0.8{\sim}1.2{\mu}m$ 크기의 간균이었다. 생리학적 특징으로서 비운동성의 통성혐기성 중온균으로서 Gram양성, catalase양성, oxidase음성, 탄수화물 발효성 등의 표현형은 Cellulomonas 속의 타종과 동일하였으며, 특히 D-ribose, raffinose, rhamnose, xylitol, acetate, L-lactate, propionate, aspartate, proline 등 조사한 모든 탄소원의 이용성이 없었으며, 반면에 sacchrose, arabinose 및 amylose의 이용성은 양성으로 판정되었다. G+C 함량 74.76 mol %, 주요 지방산과 quinone 성분은 전형적인 Cellulomonas의 12-methyltetradecanoic acid (anteiso-$C_{15:1}$)과 MK-$9(H_4)$이었으며, DNA의 상동성 비율은 C. uda ATCC 491과 70%, C. fimi ATCC 15724와 54~59 %, C. gelida 및 C. bibula와도 46~48%의 상동성을 나타내었다. 이상의 결과는 CS1-1이 현재 Cellulomonas 속에 인정된 7개의 type species 중 C. uda ATCC 491 균주와 가장 높은 근연성을 나타냄으로서 C. uda에 속하는 novel species로 분류될 수 있다.

• 미생물학회지
• /
• 제16권2호
• /
• pp.47-61
• /
• 1978

Strptomyces albus T-12 which ahd been isolated and identified in the laboratory, was selected for the studies on the cultural conditions on the production of D-xylose iosmerase and the enzymological characteristics using the partially purified enzyme. The best results in the enzyme production came from D-xylose medium than wheat bran. The divalent metla ions as $Co^{2+},\;Fe^{2+},\;Zn^{2+}\;and\;Cu^{2+}$ retard or inhibit the cell-growth at the early stages of mycelia propagations, and T-12 strain is especially sensitive to $Co^{2+}$. After 60 hours of shaking cultivation at $30^{\circ}C$ and 200 rpm, a maximum enzyme activitz, 0.49 enzyme units, was obtained. Cell-free enzyme obtained from mycelia heat-treated in the prescence of 0.5mM $Co^{2+}$, showed a 2.4-fold increase in specific than the enzyme from untreated mycelia. The specific activity of the purified enzyme through Sephadex G-150 columm showed 180 fold to the crude enzyme. The effective activators of the enzyme appeared to be $Mg^{2+}\;and\;Co^{2+}$ ions, and it exhibited the maximal enzyme activity showed at pH 7.0 and at tempersture around $80^{\circ}C$ when $Mg^{2+}\;and\;Co^{2+}$ ions were added. The enzyme isomerized D-glucose, D-xylose, D-ribose, L-arabinose, D-mannose, and L-rhamnose in the present of $Mg^{2+}\;and\;Co^{2+}$ ions as an activatiors. $Mg^{2+}\;and\;Co^{2+}$ ions were non-competitively bound at different allosterix sites of enzyme molecule. $Mg^{2+}(5mM)\;or\;Co^{2+}(1.0mM)$ protected against the thermal denaturations of the enzyme activities. The michelis constant(Km) and $V_{max}$ values of the emzyme for D-glucose and D-xylose were 0.52M, $2.12{\mu}moles/ml{\cdot}min.\;and\;0.28M,\;0.65moles/ml{\cdot}min.$, respectively.

• 생약학회지
• /
• 제34권3호통권134호
• /
• pp.246-249
• /
• 2003

The major polysaccharide, named EAP, was purified from the aerial parts of Eclipta alba by Sepharose CL-2B ion exchange chromatography and recycling HPLC. The molecular weight of EAP was estimated to be 20911.9 D by MALDI-TOF MS. In addition, the sugar composition was determined to be arabinose (23.6%), mannose (24.8%), galactose (12.3%), and glucose (41.3%), respectively, by GC analysis. The EAP decreased the blood sugar level, which was induced by alloxan in rats, dose dependently.

• Journal of the Korean Wood Science and Technology
• /
• 제15권3호
• /
• pp.34-43
• /
• 1987

This study was performed to find out the types of linkage of carbohydrates in wood cell walls. To study the structure of linkage of carbohydrates in wood cell walls, we have attempted to find out the method holocellulose preparation and optimum condition of enzyme hydrolysis in holocellulose, and fractionate oligosaccharide with products that hydrolized partly by acetolysis and deacetylation in holocellulose. We have achieved four results. These results as follow; 1. At first. we reacted in wood meal $NaClO_2$ 1g per lignin lg for one hour and then the same of quantity $NaClO_2$ for four hours. Through these experiments, we have developed new holocellulose preparation method which had low loss of carbohydrates and high effect of the delignification. 2. The optimum condition of enzyme hydrolysis of holocellulose which had lignin was 0.005M sodium acetate buffer (pH 5.0). We have achieved 7.2% reducing sugar through the procedure that reactioned 0.01g holocellulose putting enzyme 0.03g for 72 hours. It may be supposed that 5.5% of lignin contained in holocellulose prevented enzyme contaction from holocellulose and so this lignin has resulted in the low efficiency of enzyme hydrolysis. 3. We did not fractionated from oligosaccharides which were preparated by the method of acetolysis and deacetylation in holocellulose. The reason is that holocellulose having a lot of lignin prevented prefectly partial hydrolysis from the method of acetolysis and deacetylation. 4. We attempted analysis of six standard substances through HPLC apparatus having sugar pak 1 column which we have changed flow rate and the column temperature variably. These six standard substances were D-glucose, D-mannose, D-xylose, D-galactose and L-rhamnose, L-arabinose, But sugar pak 1 column was not fitted analysis of four substances because D-galactose, D-mannose, D-xylose, L-rhamnose were agreement with elution time. And so, we could not analize four standard substances with sugar pak 1 column.

• 농업과학연구
• /
• 제37권3호
• /
• pp.387-392
• /
• 2010

This studies were carried out to assess fermentation properties of amylase activity and added rice yogurt of Enterococcus faecium KHM-11 isolated from Korean human milk. The amylase activity of Enterococcus faecium KHM-11 was above 23 units. Characterization of carbohydrate fermentation of Enterococcus faecium KHM-11 has D-ribose, D-lactose, L-arabinose and starch. Titratable acidity and viable count of lactic acid bacteria of 4% rice yogurt was higher compared to the 0%. Therefore we were discussed Enterococcus faecium KHM-11 is suitable microorganism for fermented milk added rice powder. Hydrolysates of sugars of fermented milk with 4% rice powder cultured Enterococcus faecium KHM-11 were analyzed by TLC and HPLC. Hydrolysates of lactose and galactose were revealed and hydrolysates of glucose was not revealed in results of TLC and HPLC.

• 생약학회지
• /
• 제42권2호
• /
• pp.117-126
• /
• 2011

Twenty-two compounds were isolated from the 70% ethanolic extract of Rehmanniae Radix Preparata (Scrophulariaceae) and their structures were identified as three triterpenoids [oleanolic acid (1), pomonic acid (2) and ursolic acid (5)], an iridoid, catalpol (13), four furan derivatives [5-hydroxymethyl-2-furaldehyde acetate (3), 5-hydroxymethyl-2-furfural (6), 5-hydroxymethyl-2-furancarboxylic acid (7), and 5-(${\alpha}$-D-galactopyranosyloxymethyl)-2-furancarboxaldehyde (15)], three phenethyl alcohol glycosides [darendoside B (14), phenethyl alcohol 2-O-${\beta}$-D-xylopyranosyl(1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (17), and salidroside (19)], four sugar derivatives [L-arabinose (11), raffinose (20), stachyose (21), and mannitol (22)], and seven others [2,5-dihydroxyacetophenone (4), succinic acid (8), daucosterol (9), ${\beta}$-sitosterol (10), adenosine (16), uridine (18) jio-cerebroside (12)]. The chemical structures of these compounds were identified on the basis of spectroscopic methods and comparison with literature values. This is the first report of the triterpenoids oleanolic acid (1), pomonic acid (2), and ursolic acid (5) from the genus Rehmannia, as well as the first report of compounds 5-hydroxymethyl-2-furaldehyde acetate (3), 2,5-dihydroxyacetophenone (4), daucosterol (9), darendoside B (14), 5-(${\alpha}$-D-galactopyranosyloxymethyl)-2-furancarboxaldehyde (15), adenosine (16), phenethyl alcohol 2-O-${\beta}$-D-xylopyranosyl(1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (17), and salidroside (19) from the Rehmanniae Radix Preparata.

• 한국산학기술학회논문지
• /
• 제13권11호
• /
• pp.5179-5186
• /
• 2012

본 논문은 당뇨병 치료제로 알려진 Miglitol은 ${\alpha}$-glucosidase 저해제로, 산업적으로 포도당과 에탄올아민으로부터 세 단계의 화학 및 생물전환 과정을 거쳐 합성되며. acetic acid bacteria에 속하는 Gluconobactor oxydans (G. oxydans)는 불완전 산화를 통해 1-deoxy-1-(2-hydroxyethylamino)-D-glucitol (P1)을 Miglitol의 전구체인 6-(2-hydroxyetyl) amino-6-deoxy-${\alpha}$-L-sorbofuranose (P2)로 생물 전환시키는 균주이다. 본 연구에서는 토양으로부터 스크리닝하여 선발된 고효율 생물전환 박테리아인 CK-2165의 균주를 동정하고 최적의 발효조건을 탐색하고자 하였다. 16S rDNA 서열과 계통수 분석결과 CK-2165는 G. oxydans에 속하는 미생물임을 결정하였으며, API 20E kits를 사용하여 선발된 균주의 탄소원 이용성을 실험한 결과 glucose, mannose, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin, arabinose와 같은 탄소원에 대한 이용성을 가진 균주임을 확인하였다. 또한 산업적 생산을 위하여 배양 조건을 최적화하였고 균체를 이용하여 생물전환 반응에 중요한 요소들을 조사하였다. 생물전환 반응을 위해 사용되는 균체는 균 성장 단계 중 후기 정지기 (late-stationary phase)에 수확한 균체가 가장 높은 활성을 나타내었고 생물전환 반응에는 $MgSO_4$가 필수적임을 확인하였다.

• 미생물학회지
• /
• 제44권1호
• /
• pp.56-62
• /
• 2008

송이 자실체 기저부 토양으로부터 분리한 268균주를 대상으로 식물병원성 곰팡이 7균주(Alternaria panax, Botrytis cinerea, Colletotrichum gloeosprioides, Fusarium oxysporum, Phytopthora capsici, Pythium ultimum, Rizoctonia solani)에 대해 항균활성을 검토한 결과 인삼점무늬병원균(Alternaria panax)과 인삼탄저병원균(Colletotrichum gloeosprioides)에 강한 항균활성을 나타내는 KM1-15 균주가 선발되었다. KM1-15 균주의 16S rDNA 염기서열(1,311 bp)을 결정하여 계통학적 위치를 검토한 결과, Bacillus $koguryoae^T$ (AY904033)와 99.6%의 상동성을 나타내었다. API 50CHE Kit를 사용하여 당 분해능에 대해 Bacillus $koguryoae^T$ (AY904033)와 비교 검토한 결과 KM1-15 균주는 L-arabinose, cellobiose, inulin, D-turanose 양성반응을 나타내는 생리학적 특성이 확인되었다. 주요 지방산으로는 15:0 anteiso (35.78%), 17:0 anteiso (17.97%)를 함유하였으며, 13:0 iso 3-OH, 14:0 iso3-OH, 15:0 iso 3-OH, 그리고 17:0 iso 3-OH와 같은 다양한 분지형 지방산을 함유하는 특성을 나타내었다. 퀴논종으로는 MK-7 (100%)을 가지고, G+C함량은 43.7%를 나타내었다. 또한 protein과 starch에 대한 가수분해능이 탁월하여 KM1-15 균주는 병해충 방제제 및 토양개량제로 매우 유용하게 활용될 것으로 사료된다.

• Journal of the Korean Wood Science and Technology
• /
• 제10권2호
• /
• pp.12-21
• /
• 1982

Red pine and Japanese larch (Larix leptolepis Gordon) grown in Korea have been the main species of coniferous resources in Korea. Especially, planting area of Japanese larch has been increased continueously in the recent years due to its superior plant type and rapid growth rate and its stocks reached approximately 4.32 million cubic meters at the present time. Although many research works have been done for the utilization of the larch wood in various ways, still many problems are existed in its chemical applications due to a large proportion of soluble extractives. In this study, chemical composition of larch extractives and chemical structure of its major component were analyzed. In order to identify the basic structure of major component, gas-liquid chromatography for separation of some completely methylated alditols as their acetates on a 3% - ECNSS-M on Gas Chrom Q. column was used. Proportion of extractives of Japanese larch wood was higher than that of other conifers and major component of the soluble extractives was arabinogalactan, a schematic structural formula which was presented in Figure 2. The molar ratio of arabinose and galactose was 1:4.5. The main chain of arabinogalactan was composed of 1,3 linked ${\beta}$-D-galactopyranose residues, each of which carried a side chain, attached to the C-6 positions. The exact nature of all of the side chains is not known, but the majority of these side chain was composed of 1, 6 linked ${\beta}$-D-galactopyranose residues, with 2~3 such units present per average chain. Some of the galactose units in the main chain had a residue of 3 - 0 - ${\beta}$-L-arabinopyranosyl-L-arabinofuranose. In addition, a few terminal residues of D-glucuronic acid also was confirmed, attached to C-6 position of the D-galactopyranose residue. It could concluded that the main structure of highly branched arabinogalactan from Japanese larch extractive was essentially the same as those of the other larch species.