• Food Science and Preservation
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• v.9 no.4
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• pp.429-433
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• 2002

The D-xylulokinase from Candida sp. L-16 was purified through a sequence of ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-100 and Sephadex G-200 gel filtration. The specific activity of the purified Dxylulokinase was increased to 23.2 fold and the yield was 11.2%. The enzyme was showed to be a single protein band by SDS-PAGE. The molecular weight of the enzyme was 150,000 dalton, this enzyme was identified to be a dimer with two subunits. The optimum conditions of the enzyme were pH 8.0 and 40$\^{C}$, respectively. The enzyme was relatively stable between pH 7.0 to pH 9.0, but it was unstable over 30$\^{C}$. The enzyme showed substrate specificity on D-xylulose, D-arabinose and D-ribose, Km value and Vmax for D-xylulose were 0.042 mM and 117 units/ml, respectively. The activation energy of the enzyme was 4.75 Kcal/mol. The one was inhibited by metabolic intermediates such as 6-phosphogluconic acid, 2-keto-gluconic acid. The enzyme was activated by EDTA and thiol compounds such as cysteine-HCI, DTT and glutathione.

• Animal cells and systems
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• v.1 no.4
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• pp.637-640
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• 1997

Raf-1, a cytoplasmic serine/threonine protein kinase, serves as a central intermediate in many signaling pathways in cell proliferation, differentiation, and development. In this study, we investigated that B-raf, Drosophila homolog of the human c-raf-1, is involved in ultraviolet (UV) responsive events by using hypomorphic mutant $D-raf^{c110}$ and Draf-lacZ transgenic fly. At first, effect of UV damage on the survival of wild-type and $D-raf^{C110}$ strains was examined. In terms of $1/LD_{50}$ value, the relative ratio of UV sensitivities of wild-type versus $D-raf^{C110}$ strain was 1 : 2.2. By using quantitative $\beta$-galactosidase activity analysis, transcriptional activity of the D-raf gene promoter was also examined in UV-irradiated Draf-lacZ transgenic larvae. UV irradiation increased the expression of lacZ reporter gene in Draf-lacZ transgenic fly. However, in $D-raf^{C110}$ strain the transcriptional activity of D-raf gene promoter by UV irradiation was extensively reduced. Results obtained in this study suggest that D-raf plays a role in UV response, leading to better survival of Drosophila to UV damage.

• Journal of Broadcast Engineering
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• v.23 no.1
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• pp.19-25
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• 2018

The growth of virtual reality(VR), which is regarded as a part of the 4th industrial revolution, is being delayed differently from the prediction. In this study, we briefly analyze these reasons from the point of view of CPND ecosystem. Virtual reality is a technology in which a user wears a device and accesses a platform through a network to use various contents. Therefore, balanced development of C-P-N-D ecosystem fields is important for successful popularization of virtual reality. In Korea, content, platforms, and devices, except for the network, are evaluated as having low competitiveness. While efforts to secure competitiveness in individual fields are important, it is also necessary to nurture network-based platforms that can utilize killer content. It should be kept in mind that simple breeding of killer content alone can not build an entire virtual reality C-P-N-D ecosystem, and the broadcast / media sector also needs to take this approach.

• Applied Biological Chemistry
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• v.27 no.2
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• pp.129-134
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• 1984

Change of protein component in soybean sprout grown at four temperatures was investigated by polyacrylamide gel electrophoresis. Main bands were identified using purified seed globulins. Electrophoretogram showed 5 main bands (a. b, c, d, and p) and 10 minor bands in seed and maximum number (19) of bands (8 main band including 0 and 11 minor) at 4th day after germination in cotyledon. All bands appeared in axis protein but resolution was poor. In cotyledon, a component (most rapidly) and b+c+d component decreased while o+p component and other minor components were increased at 6th day and decreased thereafter. In axis all components increased rapidly, especially in minor components and b+c+d component. High growing temperature accelerated decrease in cotyledon and increase in axis of protein, especially for 11S. The a component was identified as 7S, b+c+d as 11S and o+p as 2S globulin.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2000.11a
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• pp.61-64
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• 2000

We fabricated and characterized Low Noise Amplifier (LNA) using MCM-C (Multi-Chip-Module-Cofired) technology for 2.14 GHz IMT-2000 mobile terminal application. First, We designed LNA circuits and simulated it's high frequency characteristics using circuits simulator. For the simulation, we adopted high frequency libraries of all the devices used in LNA samples. By the simulation, Gain was 17 dB and Noise Figure was 1.4 dB. We used multilayer process of LTCC (Low Temperature Co-fired Ceramics) substrate and conductor, resistor pattern for the MCM-C LNA fabrication. We made 2 buried inductors, 2 buried capacitors and 3 buried resistors. The number of the total layers was 6. On the top layer, we patterned microstrip line and pads for the SMT device. We measured the high frequency characteristics, and the results were 14.7 dB Gain and 1.5 dB Noise Figure.

• Korean Journal of Food Science and Technology
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• v.17 no.3
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• pp.192-196
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• 1985

Eight strains of Lactobacilli(a, b, b', c, d, e, f and g) were isolated from seven Korean liquid-yogurts(A, B, C, D, E, F and G), and identification and tolerance-test to digestive fluids were carried out. Isolate a from yogurt A and isolate a from yogurt E were identified as L. casei, isolate b from yogurt B as L. acidophilus, isolate d from yogurt D as L. bulgaricus, isolate f from yogurt F as L. helveticus, and isolate b' from yogurt B, isolate c from yogurt C and isolate g from yogurt G as L. jugurti, respectively. Isolate f(L. helveticus) and c(L. jugurti) showed high tolerance to artificial gastric juice but didn't to bile acid. Isolate b(L. acidophilus), a(L. casei), and e(L. casei) showed high tolerance to both artificial gastric juice and bile acid, but isolate d(L. bulgaricus), b'(L. jurgurti) and g(L. jugurti) did not.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.2
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• pp.304-313
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• 2004

Xylitol, a five-carbon natural sugar alcohol, is widely used non-cariogenic sugar substitute. In present study, the effects of xylitol on the expression of mRNA for glucosyltransferase which synthesizes glucan from sucrose were detected by Fluorescent in situ hybridization (FISH) and flow cytometry. FITC fluorescences for mRNA of gtfB, gtfC and gtfD were decreased further with increasing concentration of xylitol from 1% to 10% when detected by FISH. Flow cytometric analysis also showed that the expression of gtfB, gtfC and gtfD was increased by the addition of sucrose and decreased by the addition of xylitol to BHI broth containing 1% sucrose. In conclusion, the expression of gtfB, gtfC and gtfD mRNA was decreased by the addition of xylitol.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.12
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• pp.2609-2614
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• 2010

Temperature compensation circuit is implemented by using the temperature sensor, and Intermodulation (IM) Specification of Power Amplifier is satisfied in the temperature range of $-30^{\circ}C{\sim}60^{\circ}C$ with this temperature compensation circuit. The output voltage of temperature compensation circuit which vary 170mV with the temperature is applied to the gate of TR in 3W output power Amplifier. As the result, right 3rd IM component is -18.5~-26dBm, left 3rd IM component is -18.5~-35dBm, and the left and right 5th IM component is -24~-26dBm in the temperature range of $-30^{\circ}C{\sim}60^{\circ}C$. It is confirmed that IM specification of power amplifier which is under -17dBm in the whole temperature range is satisfied.

• Journal of the Korean Electrochemical Society
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• v.24 no.4
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• pp.113-119
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• 2021

In this study, impurity free V^3.5+ electrolytes were prepared using formic acid as a reducing agent and PtD/C as a catalyst and it was applied to VRFB. The well-oriented 3D dendrite structure of the PtD/C catalyst showed high catalytic activity in formic acid oxidation reaction and vanadium reduction reaction. As a result, the conversion ratio of electrolyte using the PtD/C was 2.73 mol g^-1 h^-1, which was higher than that of 1.67 mol g^-1 h^-1 of Pt/C prepared by the polyol method. In addition, in the VRFB charging and discharging experiment, the V^3.5+ electrolyte produced by the catalytic reaction showed the same performance as the standard V^3.5+ electrolyte prepared by the electrolytic method, thus proving that it can be used as an electrolyte for VRFB.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.3
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• pp.87-95
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• 2002

원주의 항력 감소와 원주 주위 유동의 제어에 대하여 실험적으로 연구하였다. 원주 주위 유동을 제어하기 위하여 작은 제어봉을 원주 전방에 설치하였다. 실험시 원주 직경(D=30mm)에 기초한 레이놀즈수는 $Re=2 {/times} 10^4$이었다. 제어봉의 직경(d)은 4mm, 5mm, 6mm, 7mm로 변화시켰으며, 원주와 제어봉 사이의 거리(L)를 조절하면서 원주의 표면압력분포와 후류속도를 측정하였다. 제어봉으로부터의 와 유출이 사라지는 임계거리 (critical distance) $ L_C$는 $ L_C$ /D=1.5+0.83d이었는데, $ L_C$를 전후로 유동특성과 항력값이 크게 바뀌었다. 특히 원주와 제어봉 사이의 거리가 임계거리보다 짧은 경우에는 항력이 감소하다가 $ L_C$보다 크게 되면 증가하는 경향을 보였다. 제어봉의 직경이 d/D=0.233인 경우, L/D=2.013의 조건에서 원주는 약 29%의 항력 감소를 보였고, 전체 항력은 L/D=1.833조건에서 약 25% 항력이 감소하였다.