• Title/Summary/Keyword: Cytochrome c oxidase subunit 1

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First Reliable Record of the Keeltail Pomfret Taractes rubescens (Bramidae: Perciformes) from Korea (한국산 새다래과(Bramidae) 어류 1미기록종, Taractes rubescens (Jordan and Evermann, 1887))

  • Lee, Jae-Hwan;Lee, Woo-Jun;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.3
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    • pp.283-287
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    • 2019
  • Two specimens of Taractes rubescens (family Bramidea) were collected from Busan and Pohang, Korea, between 2015 and 2016. Taractes rubescens was very similar to Taractes asper, but the two species are clearly distinguishable by the number of anal fin rays (21-23 in T. rubescens vs. 25-27 in T. asper) and the presence of a lateral caudal keel (present in T. rubescens vs. absent in T. asper). The mitochondrial DNA cytochrome c oxidase subunit I sequences of the specimens described in this paper perfectly matched those of T. rubescens, but differed from those of T. asper (genetic distance: 6.9%). We use the Korean common name proposed by Kim and Ryu (2017) for T. rubescens, "Geom-eun-sae-da-rae".

First Record of the Brackish Water Amphipod Jesogammarus (Jesogammarus) hinumensis (Amphipoda: Anisogammaridae) from Korea with DNA Barcode Analysis among Jesogammarus Species

  • Lee, Chi-Woo;Tomikawa, Ko;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.35 no.3
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    • pp.151-155
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    • 2019
  • Jesogammarus (Jesogammarus) hinumensis Morino, 1993 was discovered firstly from a brackish water region in Jeju Island, Korea. To identification of the specimens we conducted both of morphological and molecular analyses. This species is characterized by having large eyes and a robust seta on the mandibular palp article 1. The morphology of this Korean specimens was well matched with the original description without variation. The mitochondrial cytochrome c oxidase subunit I(COI) sequences of the present specimens were also completely identical to the sequences of J. (J.) hinumensis collected from the type locality of the species. Thus, we concluded that the Jesogammarus species from Jeju Island is J.(J.) hinumensis, based on both morphological and molecular data.

Philodina wonkimi n. sp. and Five New Records of Bdelloids from Korea

  • Song, Min Ok;Lee, Chang-Ho
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.4
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    • pp.363-371
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    • 2020
  • A taxonomic study on bdelloid rotifers collected from mosses and/or leaf litter at four different locations in Korea resulted in five new Korean records and a new species, Philodina wonkimi n. sp. Philodina wonkimi n. sp. is easily distinguished from its congeners by the very long antenna which is much longer than the height of the pseudosegment carrying a dorsal antenna in creeping. Among the five new Korean records, two species- and two subspecies-level taxa are new to Asia as well. Adineta rhomboidea Bērzinš, 1950 has been reported from only three European countries including the type locality, and is recorded outside Europe for the first time. Present study is the fourth record for Philodina eurystephana Schulte, 1954. In addition, a partial sequence of mitochondrial cytochrome c oxidase subunit I gene (mtCOX1) for P. wonkimi n. sp. is provided here.

A New Report of Prionospio kirrae (Annelida: Spionidae) from Korea

  • Lee, Geon Hyeok;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.2
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    • pp.91-97
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    • 2022
  • Spionid polychaete Prionospio kirrae Wilson, 1990 is newly reported from the Yellow Sea in Korea. This species is characterized by four pairs of branchiae, which are apinnate on chaetigers 2-4 and pinnate on chaetiger 5, a caruncle extending to the posterior end of chaetiger 1, the presence of a distinctly high dorsal crest on chaetiger 11, and the presence of tridentate hooded hooks with rounded apical teeth. Sequences of partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA(16S rDNA), and the nuclear 18S ribosomal DNA(18S rDNA) of the species are determined from Korean specimens.

New Record of Chinook Salmon, Oncorhynchus tshawytscha (Walbaum, 1792) (Salmoniformes: Salmonidae) from Sokcho, Korea (한국산 연어과(Salmonidae) 어류 1미기록종, Oncorhynchus tshawytscha(Walbaum, 1792))

  • Lee, Yu-Jin;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.5
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    • pp.736-741
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    • 2022
  • A single specimen (91.5 cm standard length) of the salmonid species, Oncorhynchus tshawytscha (Walbaum, 1792) was collected on April 19, 2022 in Sokcho-si, Gangwon-do, Korea. O. tshawytscha has the following morphological characteristics: base of lower jaw teeth is black, black spots are scattered on the dorsal section of body, and the edge of caudal fin is black. Although the species is morphologically similar to Oncorhynchus kisutch, O. tshawytscha differs from it in having black spots on the caudal fin. Molecular analysis results based on mitochondrial cytochrome c oxidase subunit I (COI) sequences showed that our specimen perfectly matched with the COI sequences of O. tshawytscha registered at the NCBI. Therefore, we suggest the new Korean name 'Wang-yeon-eo' for O. tshawytscha.

Morphology, Molecular Phylogeny, and Fungicide Sensitivity of Phytophthora nagaii and P. tentaculata in Korea

  • Seung Hyun Lee;Bora Nam;Dong Jae Lee;Young-Joon Choi
    • Mycobiology
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    • v.51 no.5
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    • pp.333-342
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    • 2023
  • Phytophthora species, classified under Oomycota, cause significant damage to various crops and trees. The present study introduced Phytophthora species, P. nagaii and P. tentaculata, new to Korea, which pose notable risks to their respective host plants. Our research provided a comprehensive description of these species taking into account their cultural features, morphological characteristics, and molecular phylogenetic analysis using the internal transcribed spacer rDNA region and cytochrome c oxidase subunit mtDNA genes (cox1 and cox2) sequences. In addition, this study first evaluated the sensitivity of P. nagaii and P. tentaculata to five anti-oomycete fungicides, finding both species most responsive to picarbutrazox and P. tentaculata resistant to fluazinam. The data can guide targeted treatment strategies and offer insights into effective control methods. The findings expand our understanding of the diversity, distribution, and management of Phytophthora species in Korea.

DNA Barcoding of Koreanohadra kurodana (Gastropoda: Camaenidae)

  • Kang-San Kim;Jun-Sang Lee;Kuem Hee Jang
    • Animal Systematics, Evolution and Diversity
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    • v.40 no.4
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    • pp.354-358
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    • 2024
  • The land snail, Koreanohadra kurodana (Pilsbry, 1926), is endemic to Korea and was collected from Pocheon, Pyeongchang, and Taebaek in South Korea. This study describes the mitochondrial cytochrome c oxidase subunit I gene (COI) and 16S ribosomal RNA(16S) sequences of K. kurodana, followed by an analysis of the genetic distance between the genus Koreanohadra and its congeners. As a result, intra-species variation was 0.2-3.7% in COI and 0.3-4.0% in 16S. In the gene sequences of K. koreana recently reported by Japanese researchers, both COI and 16S sequences were observed to be located within the intra-species variation of K. kurodana (1.5-3.5% in COI; 0.6-4.0% in 16S). The inter-generic variation between genera Karaftohelix and Koreanohadra, closely related, was 11.8-26% and 6.2-18.7% in COI and 16S, respectively.

Monitoring of Commercial Cephalopod Products Sold on the South Korea Market using DNA Barcode Information (DNA 바코드를 이용한 국내 유통 두족류 제품의 원재료 모니터링 연구)

  • Yu, Yeon-Cheol;Hong, Yewon;Kim, Jung Ju;Kim, Hyung Soo;Kang, Tae Sun
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.502-507
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    • 2019
  • Cephalopods are one of the most important fishery resources in the world because of their desirable taste and nutritional value. In south Korea, one of the countries in which a large amount of seafood is consumed, cephalopods (e.g., octopus, squid, and cuttlefish) have an annual consumption rate of over 400,000 metric tons. In this study, octopus and squid products (n=28) sold on the market were monitored by analyzing sequences of DNA barcode markers (cytochrome c oxidase subunit I and 16S ribosomal RNA genes). For species identification, the NCBI BLAST database was screened with the sequences and analyzed as a query. In this BLAST search, twelve squid products showed 99-100% sequence identity to Dosidicus gigas (n=3) and Todarodes pacificus (n=9). In the case of the other 16 products that were declared using octopus as raw materials on the labels, six products were identified as Cistopus taiwanicus (n=1), Amphioctopus marginatus (n=1), Scaeurgus unicirrhus (n=1), and Dosidicus gigas (n=3). Monitoring results indicated that a significant percentage (37.5%) of mislabeling was present in octopus products sold on the South Korean market.

First Reliable Record of the Dusky Shark Carcharhinus obscurus (Lesueur, 1818) (Carcharhinidae, Pisces) from Korea (한국산 흉상어과(Carcharhinidae) 어류 1미기록종, Carcharhinus obscurus (Lesueur, 1818))

  • Lee, Woo Jun;Kim, Yoonji;Na, Jung-Hyun;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.6
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    • pp.696-701
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    • 2019
  • Five specimens (839 - 1280 mm TL, Total length) of the dusky shark Carcharhinus obscurus, belonging to the family Carcharhinidae, were collected from the waters off Jejudo Island, Sinan, Namhae, Busan and Gangneung, Korea, during 2010-2019. Carcharhinus obscurus was similar to Carcharhinus brachyurus but was distinguished by the interdorsal ridge (present in C. obscurus vs. absent in C. brachyurus) and the shape of upper jaw teeth (broad in C. obscurus vs. narrow in C. brachyurus). In addition, 479 base-pair sequences in the mitochondrial DNA cytochrome c oxidase subunit I (COI) of our two specimens corresponded to those of C. obscurus (genetic distance, 0.000-0.003), but clearly distinguished from those of the Korean C. brachyurus (genetic distance, 0.03). We adopted the Korean name "Heuk-sang-eo" for Carcharhinus obscurus, after Kim and Ryu (2017).

Construction of the Full-length cDNA Library and Selection of Diapause-Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs

  • Hwnag, Jae-Sam;Go, Hyun-Jeong;Goo, Tae-Won;Yun, Eun-Young;Choi, Kwang-Ho;Seong, Su-Il;Kim, Sung-Kuk;Lee, Sang-Mong;Kim, Iksoo;Kim, Nam-Soon;Kang, Seok-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.145-148
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    • 2004
  • As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.