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DNA Barcoding of Koreanohadra kurodana (Gastropoda: Camaenidae)

  • Kang-San Kim (Insect & Invertebrate Research Team, Research Center for Endangered Species, National Institute of Ecology) ;
  • Jun-Sang Lee (Korea Mollusca Research Institute) ;
  • Kuem Hee Jang (Insect & Invertebrate Research Team, Research Center for Endangered Species, National Institute of Ecology)
  • Received : 2024.08.19
  • Accepted : 2024.10.23
  • Published : 2024.10.31

Abstract

The land snail, Koreanohadra kurodana (Pilsbry, 1926), is endemic to Korea and was collected from Pocheon, Pyeongchang, and Taebaek in South Korea. This study describes the mitochondrial cytochrome c oxidase subunit I gene (COI) and 16S ribosomal RNA(16S) sequences of K. kurodana, followed by an analysis of the genetic distance between the genus Koreanohadra and its congeners. As a result, intra-species variation was 0.2-3.7% in COI and 0.3-4.0% in 16S. In the gene sequences of K. koreana recently reported by Japanese researchers, both COI and 16S sequences were observed to be located within the intra-species variation of K. kurodana (1.5-3.5% in COI; 0.6-4.0% in 16S). The inter-generic variation between genera Karaftohelix and Koreanohadra, closely related, was 11.8-26% and 6.2-18.7% in COI and 16S, respectively.

Keywords

Acknowledgement

This research was supported by the study on interactions between habitat characteristics and life history traits of endangered insects and invertebrates (NIE-B-2024-48) from the National Institute of Ecology (NIE), Republic of Korea.

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