International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Construction of the Full-length cDNA Library and Selection of Diapause-Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs

  • Hwnag, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Go, Hyun-Jeong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Goo, Tae-Won (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Seong, Su-Il (Department of Life Science, College of Natural Science, University of Suwon) ;
  • Kim, Sung-Kuk (Department of Life Science, College of Natural Science, Daejin University) ;
  • Lee, Sang-Mong (Department of Sericulture and Entomology, Miryang National University) ;
  • Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Kim, Nam-Soon (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Seok-Woo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
  • Published : 2004.09.01
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Abstract

As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.

Keywords

References

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