• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.433-439
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• 2020

This study, which was designed to improve and increase the utilization of female elk, examined the possibility of collecting their embryos during the non-reproductive period using ultrasound-guided Ovum Pick-Up (OPU) technology. Once follicles in the ovaries of an elk were observed, the embryos were retrieved through the absorption of the follicles using an OPU ultrasound probe. Fifty-seven embryos were retrieved from 85 follicles, giving a retrieval rate of 67.1%. Morphological evaluation of the recovered embryos revealed 14.0% embryos to be in grade A, 19.2% in grade B, 15.7% in grade C, and 50.8% in grade D. The developmental efficiency of the retrieved embryos was also investigated using in vitro maturation, in vitro fertilization, and in vitro culture. After cultivating 28 embryos through in vitro fertilization, 19 embryos were found to be fertilized; the fertilization rate was 67.9%. Four embryos developed to the blastocyst stage, indicating a 14.3% development rate. This study confirmed that the production of fertilized embryos from a seasonal breeder, such as elk, is possible via the ultrasound-guided OPU method. If the efficiency of in vitro fertilization and in vitro culture can be improved through further research, it will help improve the efficiency of elk embryo production through the transplantation of their fertilized embryos.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.252-260
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• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

• 지역과문화
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• v.6 no.2
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• pp.77-104
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• 2019

This study examines the efficiency of using local cultural resources in local governments. The study does so by DEA(Data Envelope Analysis) using data from the year 2017 for 17 local governments in Korea. In addition, this study tries to estimate environmental efficiency of local cultural resources. For this, the 'Total Efficiency' including the output variables related to the local cultural resource environment was analyzed. After than It compared the 'Total Efficiency' with the 'Utilization Efficiency', to estimate the 'Environmental Efficiency' of local cultural resources. The followings are results which are significant statistically. Firstly, it was evaluated that five of the 17 local governments utilized the local cultural resources efficiently. Secondly, it was result that the inefficiency of the other local governments was relatively influenced by the economies of scale than PTE(Pure Technical Efficiency). Thirdly, It has been confirmed that environmental aspects such as cultural properties and cultural infrastructure have a considerable impact on the increase or decrease of efficiency in local governments. The difference in the efficiency of local governments are influenced by the population density. In order to improve the efficiency in the future, it is necessary to adjust the appropriate level of input according to the local population estimate, which is a major consumer of the local cultural resource utilization. In addition, the local festivals and village festivals held by local governments should be checked to improve in quality by eliminating inefficiencies. Also, it should be considered of environmental factors together, when analyzing the efficiency of the local cultural resource in local governments.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.331-339
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• 2010

Shrimp farming is the most important mariculture industry on the west coast of South Korea. However, it has suffered from mass mortality due to viral disease outbreaks and coastal pollution due to water discharge. This study developed an intensive shrimp culture method for outdoor ponds, without water exchange, which minimizes the chance of viral transmission from the environment, reduces coastal pollution by water discharge and enhances shrimp production. A culture trial was conducted in two high-density polyethylene (HDPE)-lined ponds with a $550\;m^2$ surface area. The ponds were stocked with postlarvae of Litopenaeus vannamei, the major farmed shrimp species in Korea, on July 10, 2007, and cultured for 90 days with no water exchange. The stocking density of the postlarvae (B.W. 0.0015 g) was $272\;ind./m^2$, which is eight times higher than in traditional pond culture in Korea. At harvest, the total production of ponds 1 and 2 was 1,362kg ($2.48\;kg/m^2$) and 1,282 kg ($2.33\;kg/m^2$), respectively. This is 20~22 times higher than the mean farmed shrimp production ($0.112\;kg/m^2$) in Korea and about eight times higher than in traditional ponds with a good harvest. Although there was no water exchange throughout the culture period, the mean concentrations of unionized ammonia and nitrite-nitrogen were as low as 0.038 and 6.0 mg/L, respectively. The feed conversion rate (FCR) was 1.38, which is 20~45% lower than that of traditional pond cultures. The high efficiency of the diet in this study is thought to be due to a well-managed feeding strategy and well-developed bioflocs used as diet additions for the shrimp. The final body weight of the shrimp at harvest was low (12.2~12.5 g), compared with that of traditional pond culture. This may have resulted from the combination of a short culture period, high density of shrimp, and low temperature. This study suggests that a super-intensive shrimp pond culture method using biofloc technology with no water exchange can minimize viral transmission via water exchange, reduce coastal pollution, and enhance shrimp production.

• The Journal of the Korea Contents Association
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• v.16 no.12
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• pp.667-676
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• 2016

The purpose of study was to determine the differences in organizational culture between social enterprises and commercial enterprises and assess the impact on organizational performance in Korea. Main results are as follows. First, Social enterprises employ a lot of low-imcome women and the elderly worker. Second, rational culture is high in profit businesses, but cultural group is high in social enterprises. In commercial enterprise, productivity and efficiency, planning and goal setting, assessing goals and looking for the evaluation of the performance are important. and in social enterprise, affinity and participation, employees individual development and group morale and cohesion, emphasis on mutual cooperation and trust are important. In addition, both social performance and economic performance, social enterprises are higher than in commercial enterprises. Social enterprises are operating transparently based on workers participation and understanding is expected that economic performance is also highly recognized. Third, social performance is higher development and group culture are higher in commercial enterprises, and development, group and hierarchy culture are higher in social enterprises. Economic performance is higher reasonal culture is lower in commercial enterprises, and group culture is higher in social enterprises. Therefore, the social enterprise workers are recognizing social and economic performance are higher than commercial enterprise workers. In short, social enterprises is making discriminatory organizational culture, and this is contributing to achieving organizational performance.

• International journal of advanced smart convergence
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• v.3 no.2
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• pp.1-5
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• 2014

Sensor nodes depend on batteries for energy source in Wireless Sensor Networks (WSNs). Low Energy Adaptive Clustering Hierarchy (LEACH) is a representative cluster-based routing protocol designed to ensure energy use efficiency whereas the virtual cluster exchange routing (VCR) information only with its closest node to build a network. In this paper, a protocol scheme was proposed wherein member nodes are designed to compare the currently sensed data with the previously sensed one and to switch to sleep mode when a match is achieved. The design is to help improve the transmission energy efficiency too.

• Journal of Korea Multimedia Society
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• v.19 no.5
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• pp.910-917
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• 2016

Sensors have limited resources in sensor networks, so efficient use of energy is important. Representative clustering methods, LEACH, LEACHC, TEEN generally use direct transmission methods from cluster headers to the sink node to pass collected data. However, the communication distance of the sensor nodes at low cost and at low power is not long, it requires a data transfer through the multi-hop to transmit data to the sink node. In the existing cluster-based sensor network studies, cluster process and route selection process are performed separately in order to configure the routing path to the sink node. In this paper, in order to use the energy of the sensor nodes that have limited resources efficiently, a cluster-based multi-hop routing protocol which merges the clustering process and routing process is proposed. And the proposed method complements the problem of uneven cluster creation that may occur in probabilistic cluster methods and increases the energy efficiency of whole sensor nodes.

• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.141-144
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• 2001

To enhance the shoot induction efficiency from nodal stem of yooza, the culture conditions such as basal medium, carbohydrate source, solidifying agent and the optimum concentration of plant growth regulators for shoot induction were investigated. The nodal explants were cultured better on MS medium than on MT, SH, B5 or W media in considering of shoot induction rate and mean shoot length. Solidifying agent in medium was better with 0.8% agar than with 0.3% agar, 1.2% agarose or 0.2% gelrite. Carbohydrate source in shoot induction medium was efficient with 30 g/L sucrose. The optimum concentrations of plant growth regulators were determined that 0.1 mg/L NAA as auxin was effective on the shoot induction, and 1.0 mg/L BAP as cytokinin induced multiple shoots efficiently. Shoot induction was the most effective on MS medium supplemented with 4 mg/L $GA_3$ in yooza.

• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.91-94
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• 2000

The suspension cells of Taxus cuspidata were transformed with Agrobacterium tumefaciens harboring binary vector pCAMBIE1302 encoding mgfp. Transient transfection efficiency was compared by using the fluoremetric measurement. The transient transfection efficiency was improved by transformation with DMSO and/or sonication treatment. Optimum conditions for DMSO and sonication treatment were 3% and 30sec, respectively. selection and maintenance of transformed cells were continued for 3 months. An insertion of the mgfp gene in transformed cells was detected by PCR and an expression of GFP confirmed by the western blot analysis.

• Asian Journal of Turfgrass Science
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• v.12 no.4
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• pp.195-202
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• 1998

The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.