Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Factors Affecting Efficiency of Shoot Induction in Citrus junos Sieb.

  • Kim, Mi-Hye (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lee, Hyoshin (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Chung, Min-Sup (Department of Agricultural Biology, Kyungpook National University) ;
  • Jinki Jo (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Published : 2001.09.01
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Abstract

To enhance the shoot induction efficiency from nodal stem of yooza, the culture conditions such as basal medium, carbohydrate source, solidifying agent and the optimum concentration of plant growth regulators for shoot induction were investigated. The nodal explants were cultured better on MS medium than on MT, SH, B5 or W media in considering of shoot induction rate and mean shoot length. Solidifying agent in medium was better with 0.8% agar than with 0.3% agar, 1.2% agarose or 0.2% gelrite. Carbohydrate source in shoot induction medium was efficient with 30 g/L sucrose. The optimum concentrations of plant growth regulators were determined that 0.1 mg/L NAA as auxin was effective on the shoot induction, and 1.0 mg/L BAP as cytokinin induced multiple shoots efficiently. Shoot induction was the most effective on MS medium supplemented with 4 mg/L $GA_3$ in yooza.

Keywords

References

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