• Journal of Digital Convergence
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• v.15 no.8
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• pp.33-43
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• 2017

Patent strategy research has focused on the efficiency of licensing for investigation, analysis and utilization of application management, product and development task in finding patent idea, to establish patent strategy linked with management strategy and technology strategy. However, it is difficult to find the research on patent strategy that reflects an adaptive culture to enhance the internalization and performance of the established strategy. As a result, companies have been striving to imitate patent strategies of advanced companies that do not consider their industry and organizational culture. This study proposes a method of defining a firm 's patent strategy according to the competing value model that analyzes organizational culture as four frameworks. This study verifies the classification method through case studies, suggests a suitable patent strategy for the patent strategy type. This study has contributions in that discussing from a new point of view based on organizational culture theory and providing a IP strategic model with high practicality.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.369-373
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• 2003

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.

• Magazine of the Korean Society of Agricultural Engineers
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• v.37 no.1
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• pp.55-64
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• 1995

This paper presents analytic results of energy sequestered for the forcing cultural Cu- cumber and the others production system with the input-output tables method in the suthern parts of Korea. In this study an attempt is made to evaluate input of direct and indirect energy, output of yield energy and net energy in order to achieve increased energy productivity under P E greenhouse. Cultural practices were grouped soil and soilless with perlite for vegetable production. The results from this study are summarized as follows : 1. Total energy inputs in cucumber production were calculated to be 510 GJ/l0a(di- rect energy : 480 GJ/lOa, indirect energy : 30 GJ/lOa) from soil culture and 440 GJ/ 10a(direct energy : 420 GJ/lOa, indirect energy : 20 GJ/lOa) from soilless culture in perlite hydroponics. 2. Energy outputs from cucumber and biomass were 7 GJ/lOa and 120 GJ/lOa at a uniform rate respectively. 3. Heating fuel as diesel is a major energy inputs approaching 90% of the total energy requirements for cucumber production. 4. Net energy in cucumber production was calculated to be 503 GJ/lOa from soil cul- ture and 431 GJ/lOa from soilless culture. Net energy productivity was maintained costantly as 0.98. 5. Energy productivity in cucumber was calculated to be 0.029 kg/MJ from soil culture and 0.043kg/MJ from soilless culture, while energy efficiency was 0.012 and 0.015 respectively. It is expected that a soilless cultural production system seems to be reduc- tive in seguestered energy input by 13%.

• International Journal of Computer Science & Network Security
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• v.22 no.10
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• pp.207-213
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• 2022

The article reveals the problems of aesthetic education of young people as a necessary condition for the cultural development of the individual in the modern conditions of the information society. Aesthetic education should contribute to the formation of a creatively active personality. The basis of aesthetic education of young people in the modern conditions of the information society is the core of artistic culture - art as a unique form of public consciousness that contributes to the spiritual development and improvement of the inner world of a person. The main tasks of aesthetic education are highlighted. It is focused on the formation of aesthetic consciousness and aesthetic behavior of the individual. The formation of true aesthetic and spiritual values of students is impossible without a deep awareness of the national foundations of culture, which combines science (including technology), education, art, morality, way of life and worldview, and most importantly its information component - information culture. The effectiveness of aesthetic education of students largely depends on the skillful use of various methods and means by teachers. Aesthetic education of students involves a qualitative change in the level of their aesthetic culture in the modern conditions of the information society. In the era of information and computer technologies, the main Institute for aesthetic education of young people, as a necessary condition for the cultural development of the individual, is mass media. Television stands out especially because it has several information series (audio and video sequence), multiplied by the efficiency of providing information that increases several times compared to paper media, which allows you to report directly during the event.

• Journal of Korea Trade
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• v.26 no.1
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• pp.57-80
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• 2022

Purpose - This study aims to examine the relationships between the acceptance of the 4th industrial revolution logistics technology, logistics safety behavior, and logistics safety performance, as well as the moderated mediating effects of logistics safety behavior through safety culture in Korea. Design/methodology - Research models and hypotheses were established based on prior research related to the 4th industrial revolution logistics technology, logistics safety, and logistics performance. The survey was conducted on the employees of logistics companies, and reliability analysis, confirmatory factor analysis, discriminant validity analysis, structural equation model analysis, and mediating effect analysis were performed. In addition, the moderated mediating effect analysis applying SPSS Process Model No. 7 was conducted. Findings - Usefulness and sociality of the acceptance of the 4th industrial revolution logistics technology had a significant effect on logistics safety behavior. Ease of use, sociality, and efficiency had meaningful effect on logistics safety performance. And in the relationships between the acceptance of logistics technology and logistics safety performance, logistics safety behavior had a significant mediating effect. But the moderated mediating effect of safety behavior through safety culture was not significant. Logistics companies can improve logistics safety performance through the utilization of new logistics technologies such as intelligent logistics robots, autonomous driving technology, and artificial intelligence, etc. Originality/value - This is the first study to analyze the relationships between the acceptance of logistics technology in the 4th industrial revolution and logistics safety. In addition, previous studies analyzed mediating effects or moderating effects, but this is the first study to identify the moderated mediating effects of safety behavior through safety culture. In other words, it has originality in terms of research methodology.

• ALGAE
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• v.37 no.2
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.2
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• pp.70-76
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• 2023

Background: Despite considerable technological advancements, polyspermy remains a significant challenge in in vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. Here, we aimed to determine whether optimal fertilization conditions reduce the polyspermy incidence in pigs. Methods: In vitro-matured oocytes were co-incubated with sperm according to a modified two-step culture system. Results: In the first experiment, oocytes were briefly co-incubated with sperm, washed in IVF medium, and then moved to fresh IVF medium for 5 or 6 h. Although the 6 h sperm-free cultured group had a higher penetration rate than the 5 h cultured group, the polyspermy rate significantly increased in the 6 h sperm-free cultured group. The gamete co-incubation period was either 20 or 40 min. The 40 min cultured group had a higher rate of blastocyst formation and number of total cells in blastocysts than the 20 min cultured group. In experiment 2, oocytes were inseminated with sperm separated by Pecroll treatment. Percoll treatment increased the rate of oocyte penetration and blastocyst formation compared to the control. In experiment 3, fertilized oocytes were cultured in 25 µL microdroplets (10 gametes/drop) or 500 µL (100 gametes/well) of culture medium in 4-well plates. The large volume of medium significantly reduced the number of dead oocytes and increased the rate of blastocyst formation compared to the small volume. Conclusions: Collectively, these results demonstrate that various fertilization conditions, including modified co-culture period, active sperm separation, and culture medium volume, enhance fertilization efficiency and subsequent embryonic development by decreasing polyspermy occurrence.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.3
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• pp.153-163
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• 2024

Background: The clinical application of canine mesenchymal stem cells (MSCs) necessitates efficient and safe culture methods to produce large quantities of cells. Traditionally, fetal bovine serum (FBS) has been used for MSC expansion, but it carries risks such as contamination and adverse immune responses. Methods: In this study, we investigate the efficacy and efficiency of canine allogeneic serum as an effective alternative to FBS for the in vitro culture of canine MSCs. We measured the population doubling time of canine MSCs in allogeneic serum conditions and utilized qRT-PCR, flowcytometric analysis, and cellular staining/color-metric assay for investigating its effects on cellular senescence during long-term culture and the expression of key pluripotency-related transcriptomes. Results: Our findings demonstrate that canine MSCs cultured with allogeneic serum exhibited enhanced proliferation rates, reduced cellular senescence, and lower apoptosis levels compared to those cultured with FBS. Additionally, the expression of key pluripotency-related transcription factors, including Oct4, Sox2, and Nanog, was increased in canine MSCs cultured with allogeneic serum. Conclusions: These results highlight the potential of canine allogeneic serum to provide a safer and more effective culture environment, supporting the large-scale expansion and maintenance of canine MSCs for clinical applications.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.146-149
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• 2000

Biologically active porcine Interleukin-2(poIL-2) was produced from in vitro and in viva baculovirus expression system, namely the Autographa californica nuclear polyhedrosis virus (ACNPV)-cell culture system and the Hybrid nucler polthedrosis virus (HyNPV)-sillkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.

• International Journal of Advanced Culture Technology
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• v.2 no.2
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• pp.1-6
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• 2014

In this paper, we propose an intelligent streetlight system that has a complex sensor module of temperature, humidity, luminance and motion detection and controlled by the fuzzy logic based central monitoring system in order to get flexible and precise manipulation of the streetlight system in USN environment. The proposed streetlight system provides low power consumption and high efficiency by using sensed data from the complex sensor module, which were collected, processed, and analyzed by the fuzzy logic based central monitoring system. The performance of the proposed streetlight system is to be evaluated by a simulation study in terms of power savings and safety at the fields constructed as a test-bed under several suggested scenarios. Finally, we know that the proposed intelligent streetlight system can maximize the energy savings efficiently with the fuzzy logic based central monitoring system and selective remote dimming control by connecting it to the wireless ubiquitous sensor network (USN) using a Zigbee module.