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Allogeneic serum improves the expansion and maintenance of canine mesenchymal stem cells

  • Yong-ho Choe (College of Veterinary Medicine, Gyeongsang National University) ;
  • Sang-Yun Lee (College of Veterinary Medicine, Gyeongsang National University) ;
  • Young-Bum Son (Department of Obstetrics, College of Veterinary Medicine, Chonnam National University) ;
  • Won-Jae Lee (College of Veterinary Medicine, Kyungpook National University) ;
  • Hyeonjeong Lee (College of Veterinary Medicine, Gyeongsang National University) ;
  • Chan-Hee Jo (College of Veterinary Medicine, Gyeongsang National University) ;
  • Seong-Ju Oh (College of Veterinary Medicine, Gyeongsang National University) ;
  • Tae-Seok Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Chae-Yeon Hong (College of Veterinary Medicine, Gyeongsang National University) ;
  • Sung-Lim Lee (College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2024.09.02
  • Accepted : 2024.09.11
  • Published : 2024.09.30

Abstract

Background: The clinical application of canine mesenchymal stem cells (MSCs) necessitates efficient and safe culture methods to produce large quantities of cells. Traditionally, fetal bovine serum (FBS) has been used for MSC expansion, but it carries risks such as contamination and adverse immune responses. Methods: In this study, we investigate the efficacy and efficiency of canine allogeneic serum as an effective alternative to FBS for the in vitro culture of canine MSCs. We measured the population doubling time of canine MSCs in allogeneic serum conditions and utilized qRT-PCR, flowcytometric analysis, and cellular staining/color-metric assay for investigating its effects on cellular senescence during long-term culture and the expression of key pluripotency-related transcriptomes. Results: Our findings demonstrate that canine MSCs cultured with allogeneic serum exhibited enhanced proliferation rates, reduced cellular senescence, and lower apoptosis levels compared to those cultured with FBS. Additionally, the expression of key pluripotency-related transcription factors, including Oct4, Sox2, and Nanog, was increased in canine MSCs cultured with allogeneic serum. Conclusions: These results highlight the potential of canine allogeneic serum to provide a safer and more effective culture environment, supporting the large-scale expansion and maintenance of canine MSCs for clinical applications.

Keywords

Acknowledgement

This study was supported by a grant from the National Research Foundation of Korea (Grant number: NRF-2020R1G1A1007886) Republic of Korea.

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