• Title/Summary/Keyword: Culture efficiency

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Systematic Propagation of High Quality Garlic (Allium sativum L.) Through Shoot Apical Meristem Culture III. Micropropagation by Involucre Culture (생장점배양에 의한 우량 마늘의 체계적 증식 III. 총포배양에 의한 무병주 대량증식)

  • Lee, Eun-Mo;Lee, Young-Bok
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.5
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    • pp.277-280
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    • 1994
  • This study was conducted to obtain some basic information needed for the propagational system of high quality garlic trough the culture of healthy tissues. non shoot-tips of bulbil obtained in mid May were cultured on MS medium containing 8% sucrose supplemented with 0.1 mg/L NAA, in vitro bulbli were formed, but the shoots were formed at the early to middle in June. Multiple shoots were induced by the culture of receptacles on MS medium supplemented with 0.1 mg/L NAA and 10mg/L BA..Among the flower bud, bulbil and receptacle, receptacle showed most suitable in terms of shoot formation efficiency, More than 50 shoots per single involucre were produced under the optimum condition. Results indicate that in vitro culture of involucre has a high potential for the micropropagation of high quality seed bulbs.

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Interactions between Entodinium caudatum and an amino acid-fermenting bacterial consortium: fermentation characteristics and protozoal population in vitro

  • Tansol Park;Zhongtang Yu
    • Journal of Animal Science and Technology
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    • v.65 no.2
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    • pp.387-400
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    • 2023
  • Ruminal protozoa, especially entodiniomorphs, engulf other members of the rumen microbiome in large numbers; and they release oligopeptides and amino acids, which can be fermented to ammonia and volatile fatty acids (VFAs) by amino acid-fermenting bacteria (AAFB). Studies using defaunated (protozoa-free) sheep have demonstrated that ruminal protozoa considerably increase intraruminal nitrogen recycling but decrease nitrogen utilization efficiency in ruminants. However, direct interactions between ruminal protozoa and AAFB have not been demonstrated because of their inability to establish axenic cultures of any ruminal protozoan. Thus, this study was performed to evaluate the interaction between Entodinium caudatum, which is the most predominant rumen ciliate species, and an AAFB consortium in terms of feed degradation and ammonia production along with the microbial population shift of select bacterial species (Prevotella ruminicola, Clostridium aminophilum, and Peptostreptococcus anaerobius). From an Ent. caudatum culture that had been maintained by daily feeding and transfers every 3 or 4 days, the bacteria and methanogens loosely associated with Ent. caudatum cells were removed by filtration and washing. An AAFB consortium was established by repeated transfers and enrichment with casamino acids as the sole substrate. The cultures of Ent. caudatum alone (Ec) and AAFB alone (AAFB) and the co-culture of Ent. caudatum and AAFB (Ec + AAFB) were set up in three replicates and incubated at 39℃ for 72 h. The digestibility of dry matter (DM) and fiber (NDF), VFA profiles, ammonia concentrations, pH, and microscopic counts of Ent. caudatum were compared among the three cultures. The co-culture of AAFB and Ent. caudatum enhanced DM degradation, VFA production, and Ent. caudatum cell counts; conversely, it decreased acetate: propionate ratio although the total bacterial abundance was similar between Ec and the Ec + AAFB co-culture after 24 h incubation. The ammonia production and relative abundance of C. aminophilum and P. anaerobius did not differ between AAFB alone and the Ec + AAFB co-culture. Our results indicate that Ent. caudatum and AAFB could have a mutualistic interaction that benefited each other, but their interactions were complex and might not increase ammoniagenesis. Further research should examine how such interactions affect the population dynamics of AAFB.

Digital Tools for Optimizing the Educational Process of a Modern University under Quarantine Restrictions

  • Nadiia A. Bachynska;Oksana Z. Klymenko;Tetiana V. Novalska;Halyna V. Salata;Vladyslav V. Kasian;Maryna M. Tsilyna
    • International Journal of Computer Science & Network Security
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    • v.24 no.1
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    • pp.133-139
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    • 2024
  • The educational situation, which resulted from the announced self-isolation regime, intensified the forced decisions on the organization of the distance educational process. The study is topical because of the provision of distance learning based on the experience of Kyiv National University of Culture and Arts. The study was conducted in three stages. Systemic, socio-communicative, competence approaches, sociological methods (questionnaires and interviews) were chosen as methodological tools of the research. The results of a survey of teachers and entrants to higher education institutions on the topic "Using social networks and digital platforms for online classes under the conditions of quarantine restrictions" allowed to scientifically substantiate the need for deeper knowledge of such tools as Google Meet (79%), Zoom (13.78%) and Google Classroom (11.62%), which are preferred by entrants. Almost a third of entrants (34.26%) noted the lack of scientific and methodological support for learning the subjects. The study showed high efficiency of messengers in distance education. The study found that in the process of organizing communication in the student-teacher system, it is necessary to take into account the priority of Telegram on the basis of which it is necessary to implement a chatbot for convenient and effective exchange of information about the educational process. Further research should focus on the effectiveness of the use of Telegram. The effectiveness of using chatbots should also be considered. Chatbots can be used to automate routine components of the learning process.

Production of Haploid and Doubled Haploid Plants from Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.) (고추 소포자를 이용한 반수체 및 배가반수체 생산)

  • Eun Joon Park;Yul Kyun Ahn;Doek Ho Kwon;Eun Young Yang
    • Journal of Practical Agriculture & Fisheries Research
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    • v.25 no.4
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    • pp.90-102
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    • 2024
  • Haploid/double haploid plants developed from isolated microspores can significantly accelerate plant breeding. Haploid plants can naturally double their chromosomes to create a pure homozygous line of diploid plants. We present a method for producing embryos from isolated microspores of hot peppers (Capsicum annuumL.). We analyzed the polyploidization levels of the regenerated plants. The donor plants produced the optimal stage of microspores following short-term growth under low-intensity light, which resulted in high rates of embryogenesis and cotyledonary embryogenesis. To find an efficient culture method, liquid, doubled-layer, and 2-step cultures were tested. Liquid culture yielded the highest number of embryos, whereas the highest efficiency for cotyledonary embryogenesis was afforded by the doubled-layer culture. When normal cotyledonary embryos were transplanted onto a regeneration medium, they developed into complete plants. From these, 208 plants were tested via flow cytometric analysis, and 35.6% and 72.7% of the chromosomes from the Milyang-jare and LV2319 genotypes, respectively, were found to be spontaneous double haploids. These results are the same as those obtained on analyzing horticultural characteristics, including the size of leaves and the size and shape of fruits. The present study provides information on the practical application of isolated microspore culture of hot peppers, factors that affect embryogenesis, and methods for polyploidy testing.

EFFICIENCY OF ENERGY TRANSFER BY A POPULATION OF THE FARMED PACIFIC OYSTER, CRASSOSTREA GIGAS IN GEOJE-HANSAN BAY (거제${\cdot}$한산만 양식굴 Crassostrea gigas의 에너지 전환 효율)

  • KIM Yong Sool
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.179-183
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    • 1980
  • The efficiency of energy transfer by a population of the farmed pacific oyster, Crassostrea gigas was studied during culture period of 10 months July 1979-April 1980, in Geoje-Hansan Bay near Chungmu City. Energy use by the farmed oyster population was calculated from estimates of half-a-month unit age specific natural mortality rate and data on growth, gonad output, shell organic matter production and respiration. Total mortality during the culture period was estimated approximate $36\%$ from data on survivor individual number per cluster. Growth may be dual consisted of a curved line during the first half culture period (July-November) and a linear line in the later half period (December-April). The first half growth was approximated by the von Bertalanffy growth model; shell height, $SH=6.33\;(1-e^{0.2421(t+0.54)})$, where t is age in half-a-month unit. In the later half growth period shell height was related to t by SH=4.44+0.14t. Dry meat weight (DW) was related to shell height by log $DW=-2.2907+2.589{\cdot}log\;SH,\;(2, and/or log $DW=-5.8153+7.208{\cdot}log\;SH,\;(5. Size specific gonad output (G) as calculated by condition index of before and after the spawning season, was related to shell height by $G=0.0145+(3.95\times10^{-3}{\times}SH^{2.9861})$. Shell organic matter production (SO) was related to shell height by log $SO=-3.1884+2.527{\cdot}1og\;SH$. Size and temperature specific respiration rate (R) as determined in biotron system with controlled temperature, was related to dry meat weight and temperature (T) by log $R=(0.386T-0.5381)+(0.6409-0.0083T){\cdot}log\;DW$. The energy used in metabolism was calculated from size, temperature specific respiration and data on body composition. The calorie contents of oyster meat were estimated by bomb calorimetry based on nitrogen correction. The assimilation efficiency of the oyster estimated directly by a insoluble crude silicate method gave $55.5\%$. From the information presently available by other workers, the assimilation efficiency ranges between $40\%\;and\;70\%$. Twenty seven point four percent of the filtered food material expressed by energy value for oyster population was estimated to have been rejected as pseudofaeces : $17.2\%$ was passed as faeces; $35.04\%$ was respired and lost as heat; $0.38\%$ was bounded up in shell organics; $2.74\%$ was released as gonad output, $2.06\%$ was fell as meat reducing by mortality. The remaining $15.28\%$ was used as meat production. The net efficiency of energy transfer from assimilation to meat production (yield/assimilation) of a farm population of the oyster was estimated to be $28\%$ during culture period July 1979-April 1980. The gross efficiency of energy transfer from ingestion to meat production (yield/food filtered) is probably between $11\%\;and\;20\%$.

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An Empirical Study on Public Value Conflict in Cultural Administration: Comparison and Analysis Based on Administrators, Planners, and Artists (문화행정의 공공성 가치충돌에 관한 실증연구 - 행정인, 기획인, 예술인 집단 비교분석 -)

  • Jang, Seok Ryu
    • Korean Association of Arts Management
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    • no.56
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    • pp.39-87
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    • 2020
  • This study empirically analyzed the value conflicts of cultural administration based on the needs of axiological discussions and the differences in intersubjectivity among the cultural administration groups and the contradicting attributes of culture and administration. The study classified the stakeholders into administrative staff, planners, and artists to compare their value priorities of publicness in cultural administration. A classification analysis was also conducted based on the normative by each group and the value distribution on a 2×2 value matrix between autonomy and accountability and fairness and efficiency. Based on the results of the quantitative study, the awareness of the relationships among the groups and cause and effects of value conflicts was analyzed through in-depth interviews. Thus, the study aimed to identify the directions for value distribution wherein the values of administration and culture can coexist and determine the implications of expanding this mutual understanding. The results revealed that in the conflict between autonomy and accountability, all groups had a greater awareness of accountability. In terms of normative aspects, it was possible to see a normative value line with an emphasis on autonomy, rather than on accountability from the lower stages on the budget hierarchy (administrators at the top, followed by planners and artists). In the conflict between autonomy and accountability, the size of dissonance between appropriateness and reality was the largest among the groups in the lower stages of the budget hierarchy, and became larger along the order of administrators, planners, and artists. In the conflict between efficiency and fairness, all groups had a greater awareness of efficiency. In terms of fairness in normative aspects, emphasis was placed on was artists, administrators, and planners, in that order. The size of dissonance between efficiency and fairness by groups became larger along the order of budget hierarchy-administrators, planners, and artists. Based on the results, the study compared and analyzed the 2×2 value matrix between the normative and actualities by groups. The normative value distribution emphasized Type 1 (accountability x fairness) as seeking communitarianism values through culture and Type 2 (autonomy x fairness) as seeking balanced values of cultural freedom of individualsonabalance. However, in actualities, although the communitarianism values of Type 1 were considered important, there were no distributions to the liberal values of Type 2, rather to the economic values of culture from Type 4 (accountability x efficiency). In summary, the Korean cultural administration isunderapressureof value distribution to emphasize the communitarianism and economic rather than liberal values, through bureaucratic control in actualities compared with the normative. This study will have significant implications on value distribution decision-making by groups and political implementations within the purview of cultural administration.

CALS/EC Construction for Corporate Restructuring (IMF 극복을 위한 기업의 CALS/EC 구축방안)

  • Lee, Jang-Gyoon
    • Proceedings of the CALSEC Conference
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    • 1998.10a
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    • pp.27-43
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    • 1998
  • Recently, most corporations in Korea need to construct new management system for overcoming economic crisis. Firstly, this paper suggests “Value Creation system”. Secondly, this paper suggests CALS as strategic substitute to achieve market efficiency, capital efficiency, personal efficiency, and operation efficiency, which are four critical factors of “Value Creation system”. Korean corporations should build new business model founded on cooperation by completing CALS system and reconstruct competitive management system by using knowledge and know-how accumulated in that process. Because most corporations keep exclusive corporate culture and little experience of cooperative relationship among businesses, strategic approaches are essential for construction of CALS. These are vertical integration, organization of CALS construction teams, concurrent development of product/process, CEO's iron will, Precise vision and strategies, and education/training of participant.

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The Relationship between Social Exchange, Employee Empowerment, and Organizational Efficiency in the Foodservice Industry (외식기업의 사회적 교환 관계, 임파워먼트, 조직 유효성의 관계 연구)

  • Yoo, In-Pyong;Cho, Ui-Young;Chun, Hyo-Jin
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.6
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    • pp.763-771
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    • 2006
  • This study was conducted to suggest effective alternatives for human resource management in the foodservice industry by investigating the influence of social exchange on employee empowerment and organizational efficiency, The findings derived from the study are as follows: First, the major components of social exchange, organizational support and the leader-member exchange (LMX), significantly influenced the components of employee empowerment such as signification and self-determination. Secondly, we found that signification and self-determination had a significant impact on the turnover intentions and organizational commitment of the employees, which in turn organizational efficiency. Thirdly, both the employees' turnover intentions and organizational commitment were significantly influenced by the organizational support and the leader-member exchange. These results suggest that it is necessary to have active communication between organizational leaders and members to improve employee empowerment. In other words, employees should acknowledge the meaning and importance of their tasks and support at the organizational level should be provided to ensure employee empowerment.

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A study on the influence of supply chain management efficiency on the training supporting project in small and medium-sized enterprises (중소기업 인적자원개발 지원사업에 대한 공급망경영 유효성에 관한 연구)

  • Seo, Yohan;Kim, Kwang Yong;Sung, Jong Su
    • Journal of Korean Society for Quality Management
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    • v.42 no.3
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    • pp.407-424
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    • 2014
  • Purpose: The purpose of this study was to propose supply chain management efficiency by analyzing moderating effect on the training support project in small and midium-sized enterprise. Methods: The collected data through the survey were analyzed using multi?regression analysis. The measurement tools used for this study were divided into three dimensions such as personal characteristics, supportive environment and training effect. Results: The results of this study are as follows; first, it was found that the effects of personal characteristics and supportive environment were significant on training effect. Second, significant differences were found between supply chain management active group and inactive group. Third, moderating effect of supply chain manage efficiency was found between variables. Conclusion: SME supporting project in training program needs supply chain management efficiency for training effect. Supply chain management needs to be promoted in SMEs not only for performance but also for their workplace learning and learning culture.

A Study on Escherichia Coli Disinfection by the Electrochemical Method for Small Sewerage System (소규모 오수처리를 위한 전기화학적 방법에 의한 대장균 소독에 관한 연구)

  • Park, Young-Seek;Jeong, No-Sung;Kim, Dong-Seog
    • Journal of Environmental Science International
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    • v.16 no.4
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    • pp.441-447
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    • 2007
  • This study was carried out to investigate the effect of electrochemical (EC) disinfection of artificial wastewater contaminated by Escherichia coli culture. Circulated batch type electrochemical disinfection system using three plates electrodes was used. Also, the several factors (pH, ORP, DO, temperature, current, conductivity) were measured in order to investigate the fundamental design factor in the EC disinfection system. It was demonstrated that the EC process was highly effective for wastewater disinfection. At the constant voltage, the disinfection efficiency was increased according to time. The disinfection efficiency and current increased as the increase of voltage. The variation of conductivity was a little related to the variation of CFU (colony forming units). The differences in disinfection efficiency according to the ice pack and the variation of electrodes were not occurred. The EC disinfection efficiency and current increased according to the increase of circulating flow rate.