• Title/Summary/Keyword: Culture distance

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Effect of the magnetism(neodymium magnet) on growth factor receptors of osteoblasts (희토류 자석의 자성이 골모세포 성장인자 수용체의 증가에 미치는 영향에 관한 연구)

  • Lee, Sang-Min;Lee, Sung-Bok;Choi, Boo-Byung
    • Journal of Dental Rehabilitation and Applied Science
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    • v.19 no.2
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    • pp.87-96
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    • 2003
  • The purposes of this study were to find out the optimum intensity of magnetic field where magnetism could promote the activity of osteoblast, and to discover the possibility of clinical application in the areas of dental implants and bone grafts by confirming the effect of clinically increasing bone formation. In this experiment, we used the Neodymium magnet, which had magnetic power six times as strong as the current ones and enabled the resistances against the demagnetization up to 20 to 50 times to be minimized with the size of 1mm in sight. In order to culture cells, a specially designed device was used. It was made to adjust the distance and accordingly to control the intensity of the magnetic field, by placing the cell culture plate in the center with a magnet of 1mm long and thick installed on the both ends. Using MC3T3-E1 cell, a kind of osteoblast-like cell, we cultured, for 24 hours, not only the test group which had been cultured under the magnetic fields with different intensity of 5, 10, 50, 100, 500, and 1000 Gauss, but also the control group excluding the influences of the magnetic field. After observing the cell's form and the density of the culture medium through an inverted microscope, we made a series of proceedings needed for the immunofluoroscence staining, such as fixation, normal serum reaction, primary antibody reaction, and secondary antibody reaction. And with a fluorescence microscope, we observed those-above and compared the frequency of expression of IFG-1 receptor. To make a Western immunoblotting analysis, the cells cultured under the same condition as the above had the procedure of the lysis buffer and the acrylamide gel electrophoresis was carried out. Protein transferred into the nitrocellulose membrane and tested on the primary and the secondary antibody reactions was observed and compared. The results were as follows: When observed through an inverted microscope, the nuclear divisions of the cells under the magnetic field of 10 Gauss were the most active, and the density of the cells could be observed the most enormously. As the result of an immunofluoroscence staining of IGF-1 receptor, the expression of IFG-1 was the most frequently observed under the magnetic field of 10 Gauss. On the other hand, few differences of consideration were made between the test group cultured under the magnetic fields of 5, 500, and 1000 Gauss and the control group. In respect of the expression of IFG-1 receptor, the test group cultured under the magnetic fields of 50 and 100 Gauss were higher than the control group, and lower than that cultured under the magnetic field of 10 Gauss.(p<0.05) According to the Western immunoblotting analysis, the band of IFG-1 receptor which had 85KDa of molecular weight was the darkest. Judging from the above-mentioned results, the growth factor receptor of an osteoblast cell which was an important criterion for the bone formation was increased in maximum under the magnetic field of 10 Gauss. Moreover it was observed that the optimum intensity of magnetic field in which magnetism made the activity of the osteoblast cell increase was about 10 Gauss.

In vitro Test of Mycelial Growth Inhibition of 5 Fungi Pathogenic to Strawberries by Ultraviolet-C (UV-C) Irradiation (자외선(UV-C) 조사에 의한 딸기병원균의 균사생장억제)

  • Kim, Seon Ae;Ahn, Soon-Young;Oh, Wook;Yun, Hae Keun
    • Korean Journal of Food Science and Technology
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    • v.44 no.5
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    • pp.634-637
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    • 2012
  • In strawberry production, among others, the high incidence of diseases by pathogenic fungi resulting in the reduction of fruit yield and quality requires the development of eco-friendly management systems rather than chemical sprays to control them. The diameter of colonies grown in media at $25^{\circ}C$ for 5 days was measured to evaluate the in vitro inhibition of mycelial growth of 5 pathogenic fungi by irradiation with ultraviolet (UV-C, 264 nm). The mycelial growth of 5 pathogenic fungi was inhibited in potato dextrose agar (PDA) by the irradiation of UV-C for 1 hour a day, and was dramatically inhibited by the irradiation of UV-C for 9-12 h a day. The irradiation of UV-C for 9-12 h a day inhibited completely the growth of the late blight pathogen, Phytophthora cactorum. The irradiation distance of 40 to 50 cm was effective for the inhibition of mycelial growth of fungi. The mycelial growth of fungi without pre-incubation was inhibited strongly by UV-C irradiation compared to fungi pre-incubated for 2 days without light. The mycelia growth of Colletotrichum gloeosprioides and Fusarium oxysporum was inhibited strongly by UV-C irradiation in vegetable 8 juice agar compared to PDA.

Formation of Ethnic Community the Concentrated Settlement of Foreign Workers : A Case Study of Igok-Dong, Dalseo-Gu, Daegu (외국인 밀집지역에서의 에스닉 커뮤니티의 형성 -대구시 달서구를 사례로-)

  • Jo, Hyun-Mi
    • Journal of the Korean association of regional geographers
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    • v.12 no.5
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    • pp.540-556
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    • 2006
  • The purpose of this paper is to analyze a process of formation of an ethnic community in the global era, taking an example of foreign workers in Igok-Dong, Dalseo-gu, Taegu. Previous studies suggest that playing a role as a hub of culture, resources and ethnic networks an ethnic community becomes an imagined space where its members can feel "us". Through this imagined space, ethnic people communicate and exchange information with each other and establish transnational linkages between their origin and destination countries or the third countries. In my research in Igok-Dong it was observed that ethnic shops had become the centers of the community of foreign workers and helped them connect with their own ethnic people from wider areas than their residence. Partly because of such networks exclusively focused on their own ethnics, there was little connection developed between foreign workers and locals. A social distance between the two parties may turn into antagonism as the ethnic community grows in number. Since it is foreseen that demands for foreign workers will continue to rise in Igok-Dong it is necessary to seek ways to achieve a more inclusive and harmonious multi-ethnic society for both foreign workers and locals.

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The New Urbanization Process and Urban Policy of Daegu in the 1990s (1990년대 대구의 신도시화 과정과 도시정책)

  • Kim, Soon-Cheon;Choi, Byung-Doo
    • Journal of the Korean association of regional geographers
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    • v.9 no.4
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    • pp.461-480
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    • 2003
  • This paper is to consider the new urbanization process of Daegu in the 1990s, especially with its economy, politics, social culture, environment and space. In the economic aspect, the industrial structure of Daegu has shifted from the manufacturing- centered economy to the service economy. But it has not yet overcome the linear frame of industrial structure still relying on the textile industry, though the degree of industrial specialization has increased in the part of the fabricated metal products and the precision and optical instrument. In the political situation that the implementation of the local self-government has made the relation between localities more competitive, the local government of Daegu has led a boom for boosting the regional economic growth, organizing private-public-research relations to improve the weakening regional investment and production function. In the social and cultural aspect of the new urbanization, the investment into the soft-ware facilities has been increased, and the urban festivals have been changed so as to revitalize the regional economy. In the environmental aspect, as the self-government system has launched, conflicts around values and interests of local governments have revealed frequently due to locations of infra-structures and of abhorrence facilities. Finally, seen from the spatial dimension of the city, the extension of residential areas and unregulated use of urban space have a result of inefficience of land-use, and this kind of unplanned outer expansion of the city has brought about with further separation of house and working place, and increasing distance of movements and the an urban spatial structure which requires more energy consumption.

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Characteristics of the Newly Developed Rain Shelter for Grapevine and Growth of 'Campbell Early' (신개발 포도 비가림 시설의 특성과 '캠벌얼리'의 생육)

  • Lee, Yun Sang;Kim, Seung Deok;Lee, Seok Ho;Hong, Seong Taek;Lee, Jae Wung;Hong, Eui Yon;Kim, Dae Il
    • Journal of Bio-Environment Control
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    • v.24 no.3
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    • pp.252-257
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    • 2015
  • Fruit cracking and vine leaf spot of grapes tend to occur when the plants were directly exposed to rain under outdoor culture. Rain shelter facility can be an alternative method to prevent the cracking and disease of grape, but it also has some limitations in practical usages. We designed rain shelter facility which can completely shut out the rain and ventilate naturally, and it was upgraded to meet the standards of disaster prevention against snow and wind load. The newly developed rain shelter has two-story roof structure, and the $2^{nd}$ floor roof was equipped over $1^{st}$ floor roof at a distance of 40cm. For natural ventilation and water proof, the upper roof protruded about 50cm from the ridge of a $1^{st}$ floor roof. The various tests were carried to examine such as grape quality, brown spot and fruit cracking of Campbell Early under the conventional and the newly developed rain shelter facility which was built about $100{\ss}{\check{S}}$. In comparison of temperature between the conventional and the newly developed rain shelter facility when outside temperature was more than $34^{\circ}C$, the inside temperature was recorded as $40.7^{\circ}C$ and $37.4^{\circ}C$, respectively. There was no significant difference between the two facilities when outside was below $32^{\circ}C$ The quality such as soluble solids and marketable fruit was increased, and fruit cracking of grapes and vine leaf spot also drastically diminished in the newly developed rain shelter.

A Study on the Unfair Calling under the Independent Guarantee (독립보증상의 수익자에 의한 부당청구(unfair calling)에 관한 연구)

  • Oh, Won-Suk;Son, Myoung-Ok
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.42
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    • pp.133-160
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    • 2009
  • In International trade the buyer and seller are normally separated from on another not only by distance but also by differences in language and culture. It is rarely possible for the performance of obligations to be simultaneous and the performance of contracts therefore calls for trust in a situation in which the parties are unlikely to feel able to trust each other unless they have a longstanding and successful relationship. Thus the seller under an international contract of sale will not wish to surrender documents of title to goods to the buyer until he has at least an assurance of payment, and no buyer will wish to pay for goods until he has received them. A gap of distrust thus exists which is often bridged by the undertaking of an intermediary known and trusted by both parties who will undertake on his own liability to pay the seller the contract price in return for the documents of title and then pass the documents to the buyer in return for the reimbursement. This is a common explanation of the theory behind the documentary letter of credit in which the undertaking of a bank of international repute serves as a "guarantee" to each party that the other will perform his obligations. The independence principle, also referred to as the "autonomy principle", is at the core of letter of credit or bank guarantee law. This principle provides that the letter of credit or bank guarantee is independent of the underlying contractual commitment - that is, the transaction that the credit is intented to secure - between the applicant and the beneficiary ; the credit is also independent of the relationship between the bank and its customer, the applicant. The most important exception to the independence principle is the doctrine of fraud in the transaction. A strict interpretation of the rule that the guarantee is independent of the underlying transaction would lead to the conclusion that neither fraud nor manifest abuse of rights by the beneficiary would constitute an objection to payment. There is one major problem related to "Independent guarantees", namely abusive or unfair callings. The beneficiary may make an unfair calling under the guarantee. The countermeasure of beneficiary's unfair calling divided three cases. First, advance countermeasure namely by contract. In other words, when the formation of the contract, the parties must insert the Force Majeure Clause, Arbitration Clause to Contract, and clear statement to the condition for demand calling. Second, post countermeasure namely by court. Many countries, including the United States, authorize the courts to grant an order enjoining the issuer from paying or enjoining the beneficiary from receiving payment under the guaranty letter. Third, Export Insurance. For example, the Export Credit Guarantees Department is prepared, subject to certain conditions, to cover the risk of unfair calling. Of course, KEIC in Korea is cover the risk of the all things for guarantees. On international projects, contractor performance is usually guaranteed by either a standby letters of credit or Independent guarantee. These instruments will be care the parties.

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A Study on the Utilization of 4K/UHD Camera with 2/3" Sensor : Focused on Application of HD Lens (2/3" 센서를 활용한 4K/UHD 카메라 활용방안 연구 : HD 렌즈 활용을 중심으로)

  • Kim, Sang-il;Park, Sung-chul;Kwon, Soon-chul;Lee, Seung-hyun;Hamacher, Alaric
    • Journal of Digital Contents Society
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    • v.16 no.2
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    • pp.227-234
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    • 2015
  • Recently, there is more and more demand on broadcasting display environment that provides a sense of realism basically with high quality and high definition as in UHDTV, so various kinds of 4K cameras have been released. Super 35mm sensor 4K/UHD cameras being generally used can be usefully applied to the single camera-based system such as advertisements, dramas, nature documentaries, and culture programs. However, in the multicamera-based system such as entertaining programs, sports, and concerts, relay broadcasting may face limitations as there are restrictions of close-up or tight shots according to the distance. Besides, 4K lenses are not enough compared with the previous HD lenses' group, so there are limitations in camera photographing shots that have been realized in HD filming. Therefore, to complement this, the use of 2/3 inch sensor 4K/UHD cameras is being demanded, and it is also needed to use various lenses' groups to produce UHD image contents. Accordingly, this study used Grass Valley's LDX 4K/UHD camera to analyze its applicability based on the picture quality test at the use of HD lenses in 2/3 sensors and also the possibility of its efficient application.

An Empirical Study of the Effects of Cultural Differences on Trade Scale (문화적 차이가 무역규모에 미치는 영향에 대한 실증연구)

  • Lim, Hyun-ji;Lee, Hak-loh
    • International Commerce and Information Review
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    • v.16 no.5
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    • pp.343-359
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    • 2014
  • This study investigates how cultural differences between countries affect bilateral trade volumes, using Hofstede's cultural index that reflects nations' cultural characteristics. Empirical analyses of the impacts of Hofstede's five cultural characteristics on bilateral trade volumes are conducted either in each separate equation or simultaneously. Bilateral trade data of OECD countries plus China as of year 2010 is used for regression analysis on gravity model. Regression results from individual equation for each cultural index variable show tthe smaller the index gaps of power distances and uncertainty avoidance among countries, the larger bilateral trade volumes. On the contrary, the larger the index gaps of long-term orientation among countries, the larger bilateral trade volumes. If five Hofstede cultural indexes are regressed in a single equation, however, only variables of power distance and long-term orientation are significant. The analysis largely confirms that bilateral trade among countries with similar culture have much potrential to grow. It implies that policy actions for cultural proximity are very important for furthering bilateral trade.

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Growth and Tuber Yield of Sweet Potato Slips Grown under Different Light-Emitting Diodes (LED 광질에 따른 고구마의 묘소질 및 괴근 수량성)

  • Lee, Na Ra;Lee, Seung Yeob
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.356-363
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    • 2014
  • This work was conducted to investigate the field growth and yield of the sweetpotato (Ipomoea batatas) slips grown under different light emitting diodes (LEDs). Sweet potato cuttings of 3 cultivars ('Matnami', 'Shinhwangmi', and 'Yeonhwangmi') were cultivated under fluorescent lamp (FL) and several LEDs (PPF $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at 20cm distance) in deep flow culture system for 20 days. The plants were acclimatized under sunlight for 10 days, and then cuttings (30cm length) were planted with $75{\times}25cm$ planting density on June 10th, covered with black vinyl film during growth period. Length and diameter of vine, number of root were excellent in the red plus blue (7:3) LED than the other treatments. At 30 days after planting, the survival rate in red plus blue (7:3) LED was significantly higher than that in FL and red LED, and it was not different among cultivars. Vine length, vine diameter, and number of node were not significant among LED light qualities and cultivars. After 120 days in the field cultivation, vine length, vine diameter, number of node, number of branch, and fresh weight of shoot were not significant among LED light qualities, but those except the number of branch showed significant differences among cultivars. Yield characteristics among LED light colors were not significant, but weight of storage root per plant, mean weight of storage root, and yield showed significant differences among cultivars. The yield per 10a in 'Matnami', and 'Yeonhwangmi' was significantly higher than that in 'Shinhwangmi'.

Development of RAPD Marker Related to Brown Planthopper Resistance Gene Derived from Rice Cultivar, Cheongcheongbyeo (청청벼에서 유래한 벼멸구 저항성관련 RAPD Marker의 개발)

  • Seo Ji-Hun;Kim Kyung-Min;Kim Suk-Man;Sohn Jea-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.453-456
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    • 2005
  • This study was carried out to select DNA markers closely linked to brown planthopper (BPH) resistance gene originated from a rice cultivar 'Cheongcheong­byeo'. For the mapping of resistant gene to BPH, a doubled-haploid (DH) population was developed by anther culture of $F_1$ plants from a cross 'Cheongcheong­byeo/Nagdongbyeo'. In BPH bioassay and marker screen­ing for the DH population, the segregation of resistant and susceptible plants to BPH fitted to a 1:1 ratio. A total of 310 RAPDs of 520 markers showed polymorphism in parental survey using 'Cheongcheongbyeo' and 'Nag­dongbyeo'. In the analysis of relationship between BPH resistance and marker pattern for 40 DH lines, the OPE16 produced a specific dominant fragment, 700 bp, which was closely linked with BPH resistance gene of 'Cheong­cheongbyeo'. Based on the linkage analysis using 7 markers, BPH resistance of 'Cheongcheongbyeo' was mapped on chromosome 12, which was closely linked with $OPE16_{700}$ at a distance of 4.6 cM.